In genetic and molecular breeding studies of plants, researchers need to design various kinds of primers based on their research purposes. So far many kinds of web- or script-based non-commercial programs for primer design are available. Because most of them do not include user interface for multipurpose usage including gene structure prediction and direct target selection on sequences, it has been a laborious work to design primers targeting on the exon or intron regions of interesting genes. Here we report a primer designing graphic user interface program, Pickprimer, that includes gene structure prediction and primer design modules by combining source codes of the Spidey and Primer3 programs. This program provides simple graphic user interface to input sequences and design primers. Genomic sequence and mRNA or coding sequence of genes can be copy and pasted or input as fasta or text files. Based on alignment of the input sequences using the Spidey module, a putative gene structure is graphically visualized along with exon-intron sequences of color codes. Primer design can be easily performed by dragging mouse on the displayed sequences or input primer targeting position with desirable values of primers. The output of designed primers with detailed information is provided by the Primer3 module. PCR evaluation of 24 selected primer sets successfully amplified single amplicons from six Brassica rapa cultivars. The Pickprimer will be a convenient tool for genetic and molecular breeding studies of plants.
Understanding of fracture networks and rock mass properties during tunnel construction is extremely important for the prediction of dangers during excavation, and for deciding on appropriate excavation techniques and support. However, rapid construction process do not allow sufficient time for surveys and interpretations for spatial distributions of fractures and rock mass properties. This study introduces a new statistical approach for predicting joint distributions at foreside of current excavation face during the excavation process. The proposed methodology is based on a cumulative space diagram for joint sets. The diagram displays the cumulative spacing between adjacent joints on the vertical axis and the sequential position of each joint plotted at equally spaced intervals on the horizontal axis. According to the diagram, the degree of linearity of points representing the regularity of joint spacing; a linear trend of the points indicates that the joints are evenly spaced, with the slope of the line being directly related to the spacing. The linear points which are stepped indicates that the fracture set show clustered distribution. A clustered pattern within the linear group of points indicates a clustered joint distribution. Fractures surveyed from an excavated space can be plotted on this diagram, and the diagram can then be extended further according to the plotted diagram pattern. The extension of the diagram allows predictions about joint spacing in areas that have not yet been excavated. To test the model, we collected and analyzed data during excavation of a 10-m-long tunnel. Fractures in a 3-m zone behind the excavation face were predicted during the excavation, and the predictions were compared with observations. The methodology yielded reasonably good predictions of joint locations.
Studies on target motion in 4-dimensional radiotherapy are being world-widely conducted to enhance treatment record and protection of normal organs. Prediction of tumor motion might be very useful and/or essential for especially free-breathing system during radiation delivery such as respiratory gating system and tumor tracking system. Neural network is powerful to express a time series with nonlinearity because its prediction algorithm is not governed by statistic formula but finds a rule of data expression. This study intended to assess applicability of neural network method to predict tumor motion in 4-dimensional radiotherapy. Scaled Conjugate Gradient algorithm was employed as a learning algorithm. Considering reparation data for 10 patients, prediction by the neural network algorithms was compared with the measurement by the real-time position management (RPM) system. The results showed that the neural network algorithm has the excellent accuracy of maximum absolute error smaller than 3 mm, except for the cases in which the maximum amplitude of respiration is over the range of respiration used in the learning process of neural network. It indicates the insufficient learning of the neural network for extrapolation. The problem could be solved by acquiring a full range of respiration before learning procedure. Further works are programmed to verify a feasibility of practical application for 4-dimensional treatment system, including prediction performance according to various system latency and irregular patterns of respiration.
In the case of satellite navigation positioning, the shielding of satellite signals is determined by the environment of the region at which a user is located, and the navigation performance is determined accordingly. The accuracy of user position determination varies depending on the dilution of precision (DOP) which is a measuring index for the geometric characteristics of visible satellites; and if the minimum visible satellites are not secured, position determination is impossible. Currently, the GLObal NAvigation Satellite system (GLONASS) of Russia is used to supplement the navigation performance of the Global Positioning System (GPS) in regions where GPS cannot be used. In addition, the European Satellite Navigation System (Galileo) of the European Union, the Chinese Satellite Navigation System (BeiDou) of China, the Quasi-Zenith Satellite System (QZSS) of Japan, and the Indian Regional Navigation Satellite System (IRNSS) of India are aimed to achieve the full operational capability (FOC) operation of the navigation system. Thus, the number of satellites available for navigation would rapidly increase, particularly in the Asian region; and when integrated navigation is performed, the improvement of navigation performance is expected to be much larger than that in other regions. To secure a stable and prompt position solution, GPS-GLONASS integrated navigation is generally performed at present. However, as available satellite navigation systems have been diversified, finding the minimum satellite constellation combination to obtain the best navigation performance has recently become an issue. For this purpose, it is necessary to examine and predict the navigation performance that could be obtained by the addition of the third satellite navigation system in addition to GPS-GLONASS. In this study, the current status of the integrated navigation performance for various satellite constellation combinations was analyzed based on 2014, and the navigation performance in 2020 was predicted based on the FOC plan of the satellite navigation system for each country. For this prediction, the orbital elements and nominal almanac data of satellite navigation systems that can be observed in the Korean Peninsula were organized, and the minimum elevation angle expecting signal shielding was established based on Matlab and the performance was predicted in terms of DOP. In the case of integrated navigation, a time offset determination algorithm needs to be considered in order to estimate the clock error between navigation systems, and it was analyzed using two kinds of methods: a satellite navigation message based estimation method and a receiver based method where a user directly performs estimation. This simulation is expected to be used as an index for the establishment of the minimum satellite constellation for obtaining the best navigation performance.
Welding is an essential process in the automotive industry. Most welding processes that are used for auto body are spot welding and $CO_2$ welding are used in a small part. In production field, $CO_2$ welding process is decreased and spot welding process is increased due to welding quality is poor and defects are occurred in $CO_2$ welding process frequently. But $CO_2$ welding process should be used at robot interference parts and closed parts where spot welding couldn't. Because of the 0.65mm ~ 2.0mm thickness steel sheet were used in the automotive industry, poor quality of welding area such as burn through and under fill were happened frequently in $CO_2$ process. In this paper, we will study about the penetration depth which gives a huge impact on burn through changing a degree of base metal, welding position and torch angle. Voltage, current and welding speed were fixed but degree of base metal, welding position and torch angle were changed. And Cold- Rolled(CR) steel sheet was used. Penetration depth was analysed by multiple regression analysis to derive approximate calculations. And reliability of approximate calculations were confirmed through additional experiments. As the results of this research, we confirmed the effect of torch and plate angle to bead shape. And we present a possibility that can simulate more accurate to weld geometry, as deduced the verification equations that has tolerance of less than 21.69%.
Journal of the Korean Association of Oral and Maxillofacial Surgeons
/
v.31
no.5
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pp.399-408
/
2005
In the progression of the Temporomandibular Joint Disorder(TMD), not only deformation and perforation of disc occur. But also fibrotic adhesion and inflammatory changes to the retrodiscal tissue can be seen in addition to the condylar degenerative change (e.g. osteoarthritis). However, the correct diagnosis,?planning for appropriate treatment, and prediction of prognosis are limited, because there are no means to stage the progression of the disorder. In this study relative signal intensity of retrodiscal tissue in MRI and the synovial fluid concentration of matrix metalloproteinase-2 (MMP-2), MMP-9, and Interleukin-6(IL-6) in the 23 temporomandibular joints(TMJ), from 17 patients with TMD were evaluated as a possible diagnostic marker. The relative signal intensity of retrodiscal tissue was referenced to brain gray matter with same region of interest(ROI) size. The concentrations of MMP-2, MMP-9, and IL-6 were evaluated by Enzyme Linked Immunosorbent Assay (ELISA). The collected data were compared with condylar degenerative change, joint effusion and disc position observed in MRI. The relative signal intensity of the retrodiscal tissue was increased significantly when degenerative changes were present. In addition, there was significantly high signal intensity in the presence of a disc displaced without reduction. The concentration of IL-6 was significantly increased when condylar degenerative change was no observed. And there were no changes in the levels of IL-6 according to disc position and joint effusion measurement. Moreover, there were no significant relevance between the concentration of total MMP-2 and active MMP-9 in synovial fluid, relative to degenerative changes in the mandibular condyle, to joint effusion, and to disc position observed on MRI images. In conclusion, the relative signal intensity of the retrodiscal tissue can be regarded as a mean of diagnosing the procession of TMD in a non-invasive manner. But more additional studies are required for the levels of MMP-2. MMP-9, and IL-6 to determine their potentials as a diagnostic marker for TMD.
Grounding line is used as evidence of the mass balance showing the vulnerability of Antarctic glaciers and ice shelves. In this research, we utilized a high resolution digital elevation model of glacier surface derived by recently launched satellites to estimate the position of grounding line of Campbell Glacier in East Antarctica. TanDEM-X and TerraSAR-X data in single-pass interferometry mode were acquired on June 21, 2013 and September 10, 2016 and CryoSat-2 radar altimeter data were acquired within 15 days from the acquisition date of TanDEM-X. The datasets were combined to generate a high resolution digital elevation model which was used to estimate the grounding line position. During the 3 years of observation, there weren't any significant changes in grounding line position. Since the average density of ice used in estimating grounding line is not accurately known, the variations of the grounding line was analyzed with respect to the density of ice. There was a spatial difference from the grounding line estimated by DDInSAR whereas the estimated grounding line using the characteristics of the surface of the optical satellite images agreed well when the ice column density was about $880kg/m^3$. Although the reliability of the results depends on the vertical accuracy of the bathymetry in this study, the hydrostatic ice thickness has greater influence on the grounding line estimation.
Journal of the Korean Society for Marine Environment & Energy
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v.8
no.1
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pp.17-22
/
2005
The movement of drifting ships on the sea is closely related to marine environmental forces such as waves, currents, winds, etc. To develop a prediction model for trajectories oi drifting ships, an experiment on the movement of drifting ships was carried out in the Southeastern Sea of Korea. Five types of ships including a lire raft and tour ships with G/T 10tons, G/T 2o tons, G/T 50 tons, and G/T 80 tons, were considered in the experiment. The G/T 50 ton class ship was used as a base ship for obtaining the currents, winds and heading angles of ship following the trajectory. The trajectory of each ship was measured by DGPS(Differential Global Positioning System) and collected using APRS(Automatic Position Reporting System) installed on the base ship. The error range in position fix of DGPS are approximately ±1 m. The drift speed of ship in the experiment was between 3% to 5% of wind speed and drift direction of ship was deflected by ±90° from wind direction. Also, the heading of drifting ship was normal to wind direction.
The Journal of the Institute of Internet, Broadcasting and Communication
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v.18
no.2
/
pp.227-235
/
2018
Along with the recent increase in traffic volume of vehicles, accidents involving rollover of vehicles have been rapidly increased, resulting in an increase casualties. And to prevent this, various technologies such as vehicle crash test equipment and analysis program development have been advanced. In this study, the applied vehicle model is FORD EXPLORER model, and PC-Crash program for vehicle collision analysis is used to predict the rollover accident behavior of SUV and the collision velocity. Compared with the actual rollover behavior of SUV through the FMVSS No 208 regulations, the analysis results showed similar results, the characteristics of the collision velocity and roll angle showed a tendency that the error rate slightly increased after 1000 msec. Then, as a result of considering using the database of NHTSA, it is shown that the rollover accident occur most frequently in the range of the collision velocity of 15~77 km/h and the collision angle of $22{\sim}74^{\circ}$. And it is possible to estimate the vehicle speed and collision time when the vehicle roof is broken by reconstructing the vehicle starting position, the roof failure position and the stop position by applying the actual accident case.
Journal of the Korea Academia-Industrial cooperation Society
/
v.17
no.2
/
pp.299-306
/
2016
In this paper, a method of shape prediction of an endoscope handling robot that can imitate a surgeon's behavior using a sensor network is suggested. Unit sensors, which are composed of a 3-axis magnetometer and 3-axis accelerometer pair comprise the network through CAN bus communication. Each unit of the sensor is used to detect the angle of the points in the longitudinal direction of the robot, which is made from a flexible tube. The signals received from the sensor network were filtered using a low pass Butterworth filter. Here, a Butterworth filter was designed for noise removal. Finally, the Euler angles were extracted from the signals, in which the noise was filtered by the low path Butterworth filter. Using this Euler angle, the position of each sensor on the sensor network is estimated. The robot body was assumed to consist of links and joints. The position of each sensor can be assumed to be attached to the center of each link. The position of each link was determined using the Euler angle and kinematics equation. The interpolation was carried out between the positions of the sensors to be able to connect each point smoothly and obtain the final posture of the endoscope in operation. The experimental results showed that the shape of the colonoscope can be visualized using the Euler angles evaluated from the sensor network suggested and the shape of serial link estimated from the kinematics chain model.
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