• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.12
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• pp.6159-6174
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• 2019

Recently, Kim et al. introduced the Hamming + k with m overlapped pixels data hiding (Hk_mDH) based on matrix encoding. The embedding rate (ER) of this method is 0.54, which is better than Hamming code HC (n, n - k) and HC (n, n - k) +1 DH (H1DH), but not enough. Hamming code data hiding (HDH) is using a covering function COV(1, n = 2^k -1, k) and H1DH has a better embedding efficiency, when compared with HDH. The demerit of this method is that they do not exploit their space of pixels enough to increase ER. In this paper, we increase ER using sequential Hk_mDH (SHk_mDH ) through fully exploiting every pixel in a cover image. In SHk_mDH, a collision maybe happens when the position of two pixels within overlapped two blocks is the same. To solve the collision problem, in this paper, we have devised that the number of modification does not exceed 2 bits even if a collision occurs by using OPAP and LSB. Theoretical estimations of the average mean square error (AMSE) for these schemes demonstrate the advantage of our SHk_mDH scheme. Experimental results show that the proposed method is superior to previous schemes.

• Journal of Applied Biological Chemistry
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• v.43 no.1
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• pp.5-11
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• 2000

Three cDNA clones encoding rice calmodulin (CaM) isoforms (OsCaM-1, OsCaM-2, and OsCaM-3) were isolated from a rice cDNA library constructed from suspension-cultured rice cells treated with fungal elicitor. The coding regions of OsCaM-1 and O.sCaM-2 were 89% homologous at DNA Ievel, whereas the 5' and 3' untranslated regions were highly divergent. The polypeptides encoded by OsCaM-1 and OsCaM-2 was identical except two conservative substitution at position 8 and 75. The coding region of OsCaM-3 was consist of a typical conserved CaM domain and an additional C-terminal extension. The amino acid sequence of conserved CaM domain of OsCaM-3 shared only 86% identity with that OsCaM-1. The OsCaM-3 cDNA is belongs to a novel group of calmodulin gene due to its C-terminal extension of 38 amino acids, a large number of which are positively charged. The extension also contains a C-terminal CaaX-box prenylation site (CVlL). Genomic Southern analysis revealed at least six copies of CaM or CaM-related genes, suggesting that calmodulin may be represented by a small multigene family in the rice geneme. Expression of OsCaM gene was examined through Northern blot analysis. Transcript level of OsCaM-3 was increased by treatment with a fungal elicitor, whereas the OsCaM-1 and OsCaM-2 genes did not respond to the fungal elicitor. The expression of OsCaM-3 gene was remarkable inhibited in the rice cells treated with cyclosporine A, calcinurin inhibitor.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.1 no.3
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• pp.97-103
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• 2008

In this paper, $256{\times}256$ input image is classified into a validity block and an edge block of $8{\times}8$ block for image compression. DCT(Discrete Cosine Transform) is executed only for the DC coefficient that is validity coefficients for a validity block. Predict the position where a quantization coefficient becomes 0 for an edge block, I propose new algorithm to execute DCT in the reduced region. Not only this algorithm that I proposed reduces computational complexity of FDCT(Forward DCT) and IDCT(Inverse DCT) and decreases encoding time and decoding time. I let compressibility increase by accomplishing other stability verticality zigzag scan by the block size that was classified for each block at the time of huffman encoding each. In addition, the algorithm that I suggested reduces Run-Length by accomplishing the level verticality zigzag scan that is good for a classified block characteristic and, I offer the compressibility that improved thereby.

• Journal of the Korean Wood Science and Technology
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• v.34 no.3
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• pp.56-63
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• 2006

In order to evaluate the mechanism of cellulose hydrolysis, the complementary DNA encoding Glycoside Hydrolase Family (GHF)74 was cloned from Phanerochaete chrysosporium. Depending on the presence of Cellulose Binding Module (CBM), it can be classified as GHF74A or GHF74B. The GHF74A gene from P. chrysosporium (PcGHF74A) consists of 2163 bp encoding a protein of 721 amino acid residues. The PcGHF74A showed homology of 70~77% compared with the GHF74 from other filamentous fungi. The PcGHF74B, which contains CBM and is a member of family 1, was transcribed to various transcripts depending on the nature of carbon sources and their concentration. To study the possible presence of splice variants in GHF74B transcripts in P. chrysospoium, we carried out RT-PCR analysis using primers that designed based on the annotation data and sequenced data. Our result indicated that PcGHF74B was transcribed to several splicing variants in various culture conditions. Especially in the culture of 2% cellulose, three transcript products were observed. First transcript was presumed to be a full length ORF that contained 11th intron with stop codon at position 2562 bp. The second one consisted of 12 exons and 11 introns with stop codon at position 1187 bp with 7th exon. The shortest transcript consisted of 10 exons and 9 introns with stop codon at 910 bp in the 7th exon. These premature stop codon might prevent the synthesis of fully active GHF74 or contribute for the production of protein with distinct function depending on the ambient carbon sources.

• Progress in Medical Physics
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• v.21 no.1
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• pp.93-98
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• 2010

The aim of this work was to establish the methodology for event positioning by measuring depth of interaction (DOI) information and to evaluate the system sensitivity and spatial resolution of the new detector for I-125 and Tc-99m imaging. For this purpose, a Monte Carlo simulation tool, DETECT2000 and GATE were used to model the energy deposition and light distribution in the detector and to validate this approach. Our proposed detector module consists of a monolithic CsI(Tl) crystal with dimensions of $50.0{\times}50.0{\times}3.0\;mm^3$. The results of simulation demonstrated that the resolution is less than 1.5 mm for both I-125 and Tc-99m. The main advantage of the proposed detector module is that by using 3 mm thick CsI(Tl) with maximum-likelihood position-estimation (MLPE) method, high resolution I-125 imaging and high sensitivity Tc-99m imaging are possible. In this paper, we proved that our new detector to be a reliable design as a detector for a multi-energy SPECT.

• Journal of the Korean Society of Radiology
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• v.13 no.3
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• pp.319-324
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• 2019

A depth-encoding detector module with silicon photomultipliers(SiPMs) using two layers of scintillation crystal array was designed, and the position measurement capability was verified using DETECT2000. The depth of interaction of the crystal pixels with the gamma rays was tracked through the image acquired with the combination of surface treatment of the crystal pixels and reflectors. The bottom layer was treated as a reflector except for the optically coupled surfaces, and the crystals of top layer were optically coupled each other except for the outer surfaces so that the light sharing was made easier than the bottom layer. Flood images were obtained through the combination of specular reflectors and random reflectors, grounded and polished surfaces of crystal pixels, and the positions at which layer images were generated were measured and analyzed. The images were reconstructed using the Anger algorithm, whose the SiPM signals were reduced as the 16-channels to 4-channels. In the combination of the grounded surface and all reflectors, the depth positions were discriminated into two layers, whereas it was impossible to separate the two layers in the all polished surface combinations. Therefore, using the combination of grounded surface crystal pixels and reflectors could improve the spatial resolution at the outside of the field of view by measuring the depth position in preclinical positron emission tomography.

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.381-388
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• 2001

The present study was designed to obtain porcine growth hormone binding protein (pGHBP) improved biological activation as derived mutation in binding site with growth horlnone (GH). A 756 bp of fragment encoding the extracellular domain of pGHBP gene was cloned from the total RNA of porcine fat tissue by reverse transcriptase polymerase chain reaction (RT-PCR) and created mutation in positions 26 and 122 using site-directed mutagenesis method. Position 26 is one and it is near to get on five potential N-linked glycosylation sites located in the extracellular domain of porcine growth hormone receptor known to have a direct influence on combination with GH. Position 122 is known as one of conformational epitope in bovine. It was over-expressed in E. coli using pET-32(c) expression vector and precisely purified by S-protein agarose and enterokinase. In our results, we was obtained pmGHBP of 30 kDa. It suggests to study the effects of the pmGHBP on cell proliferation in vitro and growth rate in vivo after administration.

• Journal of the Institute of Convergence Signal Processing
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• v.20 no.2
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• pp.84-90
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• 2019

Tremendous computation of full search or lossless motion estimation algorithms for video coding has led development of many fast motion estimation algorithms. We still need proper control of computation and prediction quality. In the paper, we suggest an algorithm that reduces computation effectively and controls computational amount and prediction quality, while keeping prediction quality as almost the same as that of the full search. The proposed algorithm uses multiple thresholds for partial block sum and times of counting unchanged minimum position for each step. It also calculates the partial block matching error, removes impossible candidates early, implements fast motion estimation by comparing times of keeping the position of minimum error for each step, and controls prediction quality and computation easily by adjusting the thresholds. The proposed algorithm can be combined with conventional fast motion estimation algorithms as well as by itself, further reduce computation while keeping the prediction quality as almost same as the algorithms, and prove it in the experimental results.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.7 s.361
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• pp.45-53
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• 2007

In this paper, we propose an efficient hardware architecture for H.264/AVC CAVLC (Context-based Adaptive Variable Length Coding) encoding. Previous CAVLC architectures search all of the coefficients to find statistic characteristics in a block. However, it is unnecessary information that zero coefficients following the last position of a non-zero coefficient when CAVLC encodes residual coefficients. In order to reduce this unnecessary operation, we propose two techniques, which detect the first and last position of non-zero coefficients and arrange non-zero coefficients sequentially. By adopting these two techniques, the required processing time was reduced about 23% compared with previous architecture. It was designed in a hardware description language and total logic gate count is 16.3k using 0.18um standard cell library Simulation results show that our design is capable of real-time processing for $1920{\times}1088\;30fps$ videos at 81MHz.

• Microbiology and Biotechnology Letters
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• v.25 no.1
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• pp.23-29
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• 1997

The nucleotide sequence of the estI gene encoding acetylxylan esterase I of Bacillus stearothermophilus was determined and analyzed. The estI gene was found to consist of a 810 base pair open reading frame coding for a polypeptide of 270 amino acids with a deduced molecular weight of 30 kDa. This was in well agreement with the molecular weight (29 kDa) estimated by SDS-PAGE of the purified esterase. The coding sequence was preceded by a putative ribo some binding site 10 bp upsteam of the ATG codon. Further 53 bp upstream, the transcription initiation signals were identified. The putative $_{-}$10 sequence (TCCAAT) and $_{-}$35 seqence (TTGAAT) corresponded closely to the respective consensus sequences for the Bacillus subtiis major RNA polymerase. The G+C content of the coding region of the estI was 51% whereas that of the third position of codone was 60.2%. The N-terminal amino acid sequence of the EstI deduced from the nucleotide sequence perfectly matched the corresponding region of the purified esterase described previously. Comparison with the amino acid sequence of other esterases and lipases reported so far allowed us to identify a sequence, GLSMG at positions 123 to 127 of the EstI which was reported to be the highly conserved active site sequence for those enzymes. The nucleotide sequence of the estI revealed 55.7% homology to that of the xylC coding for the acetylxylan esterase of Caldocellum saccharolyticum.