• Journal of Life Science
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• v.6 no.4
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• pp.299-303
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• 1996

The effects of ozone (O$_{3}$) exposures on chlorophyll content of trembling aspen(Populus tremuliodes) rooted cuttings were studied. Ozone was treated with 100 ppb fumigation for 6 hours per day, for three consecutive days per week in open-top chamber. The ozone exposures caused severe foliage damage expressed in necrotic patches and extensive chlorosis. Degradation of chlorophyll was observed mostly in mature leaves. The chlorophyll response to ozone exposure maintained a similar pattern during all experimental seasons and plant materials, Significant genetic variation in O$_{3}$ responses was revealed by differences in sensitivies among clones.

• Genes and Genomics
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• v.40 no.11
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• pp.1127-1136
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• 2018

The Greater Sudbury Region has been known as one of the most ecologically disturbed areas in Canada for the past century. Plant adaptation to environmental stressors often results in modifications in gene expression at the transcriptional level. The main objective of the present study was to compare the expression of genes associated with nickel resistance in Acer rubrum and Populus tremuloides growing in areas contaminated and uncontaminated with metals. Primers targeting Nramps4, Nas 3, At2G, MRP4 and alpha-tubulin genes were used to amplify cDNA of both species. The expression of the At2G gene, was $2{\times}$ and $9{\times}$ higher in P. tremuloides than in A. rubrum for St. Charles (uncontaminated site) and Kelly Lake (metal contaminated site), respectively. There was a much smaller difference between the two species for the Nramps 4 gene as its expression was $2.5{\times}$ and $3{\times}$ higher in P. tremuloides compared to A. rubrum from St. Charles and Kelly Lake, respectively. The same trend was observed for the MRP4 gene whose expression was $2{\times}$ and $14{\times}$ higher in P. tremuloides than in A. rubrum from St. Charles and Kelly Lake, respectively. For the Nas 3 gene, the expression was similar in both sites. This gene was upregulated $11{\times}$ and $10{\times}$ in P. tremuloides compared to A. rubrum in samples from St. Charles and Kelly Lake, respectively. In general, no significant difference was observed between the metal contaminated and uncontaminated sites for gene expression. In depth analysis revealed that AT2G and MRP4 genes were significantly down regulated in A. rubrum from the metal contaminated sites compared to those from uncontaminated areas, but environmental factors driving this differential gene expression couldn't be established.

• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.20-25
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• 1984

The inheritance of isoenzymes in young coat tissues of trembling aspen (Populus tremuloides Michx.) was studied by electrophoretically analysing five parental clones and their full-sib progenies obtained by crossing one female clone to four male clones. The distal 2cm section of vigorous young roots of 70 seedlings per family were subjected to horizontal starch gel electrophoresis. The resulting gels were tested for activity of 7 enzyme systems. Evidences for the inheritance of isoenzymes observed indicated that the isoenzyme variants of every isoenzyme zone were under control of codominant alleles at a single locus. Chi-square test of joint segregation data of the two loci, 6-PGD-2 and PGI-2, indicated that the pair of loci was not linked.

• Journal of Korean Society of Forest Science
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• v.83 no.3
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• pp.372-379
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• 1994

Timber harvesting impacts on soil microbial populations and respiration rates were examined in naturally regenerating trembling aspen(Populus tremuloides Michx.) stands on two contrasting soils, an Omega loamy sand (sandy mixed, frigid Typic Udipsamment) and an Ontonagon clay loam (very fine, mixed Glossic Eutroboralf). Five timber harvesting disturbances were simulated during winter of 1990 and spring of 1991, including commercial whole-tree harvesting(CWH), winter logging trail+CWH, logging slash removal+CWH(LSR), forest floor removal+LSR(FFR), and spring compaction+FFR. Regardless of soil types, total soil respiration rates of each stand decreased slightly or remained the same after harvesting while microbial population increased progressively during the first two years following harvesting. Microbial populations increased more rapidly and constantly at the sandy site than at the clayey site, which may indicate that the soil physical and chemical conditions changed more drastically for microbial activity following timber harvesting at the sandy site than at the clayey site. However, two kinds of treatment applications-three levels of organic matter removal and two levels of compaction-did not result in significant differences in microbial population or total soil respiration rate at each site during the first two post-harvest years. Total soil respiration of the aspen stands, sum of root respiration and microbial respiration, was a poor index for the microbial activity in this study because aspen kept an active root system for the successive root-sprouts even after harvesting, which resulted in a large portion of root respiration in total respiration.

• Journal of the Korean Wood Science and Technology
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• v.33 no.6 s.134
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• pp.79-86
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• 2005

Aspen wood (Populus tremuloides, L.) was biotreated with Ceriporiopsis subvermispora for 1, 2, 4, and 6 weeks to observe the physical/chemical modification of wood components. Milled wood lignins (MWLs) isolated from each decayed wood were analyzed by gel permeation chromatography (GPC) and nitrobenzene oxidation (NBO). As fungal treatment was progressed, lignin contents continuously decreased up to 20% after 6-week treatment. The lignin polymer could be fragmented to low-molecular phenolics, which make an enhancement of alkali solubility. Holocellulose contents were not affected severely during the period of fungal treatment, only reduction of 5~6% compared to the control. Xylose contents were decreased gradually from 23.4% to 18% after 6 weeks, whereas alpha-cellulose remained almost unchanged. Gel permeation chromatography (GPC) indicates that molecular weight of lignin undergoes a slight decrement for 4 weeks of fungal treatment. Nitrobenzene oxidation revealed that total yield of NBO products of lignins were lowered ca 20% after fungal treatment. Sum of syringaldehyde and syringic acid are remarkably decreased. However, increment of sum of vanillin and vanillic acid was surprisingly observed. These results work as indirect evidence that a specific lignolytic reaction, maybe selective demethoxylaytion of S-lignin, can occur during fungal treatment of aspen wood by C. subvermispora.

• Journal of Plant Biotechnology
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• v.3 no.3
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• pp.131-134
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• 2001

In $\lambda$-Zap II vector system, a cDNA library was constructed for the developing secondary xylem mRNA from hybrid poplar, Populus nigra x maximowiczii. A cDNA clone of 1.5 kb in size, pOMTB1.4 encoding a lignin-bispecific O-methyltransferase was screened by plaque hybridization using a probe of 540 bp cDNA amplified by polymerase chain reaction from the cDNA library and identified by nucleotide sequencing. Its nucleotide sequence contains one open reading frame of 366 amino acids. The deduced amino acid sequence in comparison with that of Populus tremuloides showed the differences of 9 amino acids and revealed 85-99% homology among alfalfa, poplar and aspen.

• The Korean Journal of Ecology
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• v.10 no.3
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• pp.119-127
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• 1987

On the east slope of the front ranges of the Colorado Rockies, USA, three species of Populus are distributed at different elevations from the Great Plains to the timberline. At elevations 1, 720m (P. sargentii), 2, 335m (P.angustifolia) and 3, 190m (P.tremuloides) chlorophyll cotents of leaves of thses dioecious trees were determined. Total chlorophyll content was in the range of 0.86-1.56mg/g leaf, increasing with elevation as a second-degree polynomial, and the peak content was estimated to be reached at about 2, 800m. Total chlorophyll content was greater in female than in male trees in the two higher elevation species, but not in P. sargentii, the low elevation species. Chlorophyll b content was greater than chlorophyll a content in the same two higher elevation species. The increase of chlorophyll b than to increase of chlorophyll a content. These phenomena appear to be the result of adaptation of these species to different environmental conditional at different elevation. Sequential distribution of plant populations on environmental gradients in such a way that variation in given plant characters paralell the environmental gradient must occur not only in ecotypes of a species, but also in different but closely related species.

• Applied Biological Chemistry
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• v.38 no.2
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• pp.123-128
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• 1995

Several factors affecting on transformation efficiency were studied to establish a Agrobacterium rhizogenes mediated system for the transformation of Populus species, and We could obtaine tansgenic plantlets expressing the introduced gene. Leaf sections were more sensitive than stem sections to kanamycin and thought to be better material for transformant screening. The bacterial density did not affect on the efficiency of transformation over the range of $4{\times}10^5{\sim}7{\times}10^9\;cfu$. The optimum period for co-cultivation was one day or shorter. Both of the optimum concentrations of cefotaxime and ampicillin in the medium were $250\;{\mu}g/ml$ for elimination of bacteria from the inoculated leaf sections. The addition of acetosyringone in the bacterial culture medium increased transformation rate, and the highest rate was obtained at $50\;{\mu}M$ of acetosyringone. The transformed galls could be selectively induced and gown on the growth regulator-free medium or on the medium containing $100\;{\mu}g/ml$ or higher contrition of kanamycin. The roots were induced from the galls incited by A. rhizogenes within 3 weeks on the growth regulator-free medium as well as on the medium containing growth regulators. The plantlets were regenerated from the galls cultured for 6 weeks on the medium containing 0.05 mg/ml of NAA and 0.5 mg/ml of BA. The expressions of the introduced opine gene in the transformed galls and plantlets were confirmed by the analysis of agropine and mannopine.

• The Plant Pathology Journal
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• v.15 no.4
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• pp.210-216
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• 1999

TREGRO, a computer simulation model of individual tree growth, was applied to estimate ozone ($\textrm{O}_3$) effects on aspen(Populus tremuloides) growth under ambient and 1.7 times ambient $\textrm{O}_3$ of Seoul in 1996. The three highest $\textrm{O}_3$ (Kuui-dong, Ssangmun-dong, Sungsoo-dong) and the two lowest $\textrm{O}_3$ sites (Mapo-dong, Namgajwa-dong) were evaluated. The current ambient $\textrm{O}_3$ did not affect aspen growth compared to simulation without $\textrm{O}_3$. The only effect was 6.6 percent of total assimilated carbonloss at Ssangmun-dong where the level of $\textrm{O}_3$ was greatest among the 21 sites examined. Decrease as much as 50 percent of total carbon gain was calculated at 1.7 times ambient $\textrm{O}_3$ of the three highest sites. The carbon loss by $\textrm{O}_3$ came from biomass of tissues and total nonstructural cabron (TNC) such as starch and sugar. The most sensitive fraction was TNC and the next was root biomass. Foliage mass was not affected by $\textrm{O}_3$. Structural biomass loss was at best 1 to 3 percent at 1.7 times ambient $\textrm{O}_3$ at the two lowest sites. The daily carbon simulation was affected by $\textrm{O}_3$ mainly during Growth Period 4 (Jul. 21-Oct. 26). Correlations between site, dose, and the simulated responses of aspen (tissue biomass, TNC, respiration, and senescence) ranged from -0.703 to -0.973 depending on the plant responses. The ozone effects on poplar in Seoul are not severe currently, but are probably measurable at Ssangmun-dong. However, severe $\textrm{O}_3$ effects on biomass would occur if $\textrm{O}_3$ levels increase to 1.7 times ambient $\textrm{O}_3$ in Seoul. In addition, v could weaken the trees thus increasing susceptibility to pathogens or insects.

• Journal of Korean Society of Forest Science
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• v.85 no.4
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• pp.656-666
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• 1996

Aspen demand has increased recently in the Great Lakes region in the United States. Since aspen has moved into the region in late 1800's, its growing stock has increased so as to change forestry industry of the Lake States. Intensive timber harvesting and biomass removal may cause nutrient depletion, especially on nutrient-poor sites. Forest nutrients and nutrient cycling were investigated in aspen stands of 7-10, 27-33, and 41-42 year-old growing on sandy soils in Minnesota. Nutrients added to the aspen stands by atmospheric deposition and soil weathering were efficiently absorbed and stored in the tree biomass. Aboveground biomass increased from $24.4t{\cdot}ha^{-1}$ at young stands to $139.2t{\cdot}ha^{-1}$ at mature stands. Nutrients accumulated in the tree biomass showed same magnitude of difference. Nutrients added to the site through atmospheric deposition were in the order of Ca, N, K, Mg, and P. Annual litterfall was greater in older stands. However, the amount of nutrients returned by litterfall was not significantly different among stand ages due to the greater nutrient contents in the litterfall of young stands. Litter decomposition and nutrient release rates were greater at young stands than at older stands. Likewise, nutrient availability was higher in young aspen stands and became lower as the stands grew older. Nutrient leaching loss was minimal at all stand ages. Soil N mineralization was greater at young stands than at older stands. Nutrient cycling process was facilitated in young aspen stands with an increased level of available nutrients, Based on the estimations of nutrient balance and nutrient removal by harvesting, Ca was the most critical element which was likely to be depleted if aspen stands are intensively harvested with short rotations.