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Structural Analysis of Milled Wood Lignins Isolated From Aspen Wood (Populus tremuloides L.) Biotreated by Ceriporiopsis subvermispora  

Choi, Joon-Weon (Division Wood Chemistry & Microbiology, Korea Forest Research Institute)
Moon, Sung-Hee (College of Life & Environmental Science, Korea University)
Ahn, Sye-Hee (Division Life & Environmental Resources, Daegu University)
Choi, Don-Ha (Division Wood Chemistry & Microbiology, Korea Forest Research Institute)
Paik, Ki-Hyun (College of Life & Environmental Science, Korea University)
Publication Information
Journal of the Korean Wood Science and Technology / v.33, no.6, 2005 , pp. 79-86 More about this Journal
Abstract
Aspen wood (Populus tremuloides, L.) was biotreated with Ceriporiopsis subvermispora for 1, 2, 4, and 6 weeks to observe the physical/chemical modification of wood components. Milled wood lignins (MWLs) isolated from each decayed wood were analyzed by gel permeation chromatography (GPC) and nitrobenzene oxidation (NBO). As fungal treatment was progressed, lignin contents continuously decreased up to 20% after 6-week treatment. The lignin polymer could be fragmented to low-molecular phenolics, which make an enhancement of alkali solubility. Holocellulose contents were not affected severely during the period of fungal treatment, only reduction of 5~6% compared to the control. Xylose contents were decreased gradually from 23.4% to 18% after 6 weeks, whereas alpha-cellulose remained almost unchanged. Gel permeation chromatography (GPC) indicates that molecular weight of lignin undergoes a slight decrement for 4 weeks of fungal treatment. Nitrobenzene oxidation revealed that total yield of NBO products of lignins were lowered ca 20% after fungal treatment. Sum of syringaldehyde and syringic acid are remarkably decreased. However, increment of sum of vanillin and vanillic acid was surprisingly observed. These results work as indirect evidence that a specific lignolytic reaction, maybe selective demethoxylaytion of S-lignin, can occur during fungal treatment of aspen wood by C. subvermispora.
Keywords
Ceriporiopsis subvermispora; Populus tremuloides; L., biodegradation; nitrobenzene oxidation; MWL; GPC; alkali solubility;
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