• Journal of the Korean Wood Science and Technology
• /
• v.19 no.1
• /
• pp.64-70
• /
• 1991

In order ot obtain the information on wood quality of the Populus nigra $\times$ maximowiczii the distributions of crystalliferous substancess, ty loses and tension woods were examined through light microscopy or scanning electron microscopy. 1. The crystalliferous subtance, presumably calcium oxalate. partially occurred in vessel elements of heartwood, and sporadically in ray parenchyma, but not in the wood fibers The types of crystalliferous subtances were mainly crystal sand, $2-3{\mu}m$ in diameter. 2. The tyloses arranged in a ladder-like series were sporadically formed in the vessel elements of heartwood. 3. Although the woods were cut from the straightly grown stem without any evident disorientation with respect to gravitational axis, single or group of gelatinous fibers were irregularly distributed among normal wood fibers.

• Journal of Plant Biotechnology
• /
• v.3 no.3
• /
• pp.131-134
• /
• 2001

In $\lambda$-Zap II vector system, a cDNA library was constructed for the developing secondary xylem mRNA from hybrid poplar, Populus nigra x maximowiczii. A cDNA clone of 1.5 kb in size, pOMTB1.4 encoding a lignin-bispecific O-methyltransferase was screened by plaque hybridization using a probe of 540 bp cDNA amplified by polymerase chain reaction from the cDNA library and identified by nucleotide sequencing. Its nucleotide sequence contains one open reading frame of 366 amino acids. The deduced amino acid sequence in comparison with that of Populus tremuloides showed the differences of 9 amino acids and revealed 85-99% homology among alfalfa, poplar and aspen.

• Journal of Korean Society of Forest Science
• /
• v.58 no.1
• /
• pp.54-59
• /
• 1982

Powdery mildew has been observed on the several clones of Populus euramericana, P. deltoides, P. deltoides ${\times}$ P. maximowiczii in the nurseries of Chuncheongan-do and Kangwoen-do forest Research Institute in mid-October 1982. Powdery mildew fungus of Populus spp. was identified as phyllactinia guttata(Waller. ex Fr.) $L{\acute{e}}v$ which was reported as the powdery mildew fungus of Populus tomentosa Carr. Powdery mildew of Populus spp. caused by this fungus has not previously been reported in Korea. When we surveyed the occurrence of powdery mildew disease the leaves of P. deltoides R-89 were severely attacked by this fungus.

• Journal of Plant Biotechnology
• /
• v.3 no.3
• /
• pp.135-140
• /
• 2001

An Agrobacterium tumefaciens LBA4404 (harboring antisense OMT gene)-mediated transformation method has been developed for poplar (P.nigra x maximowiczii) using prolonging co-cultivation time. Explants on LT (longterm) were induced transgenic calli one month earlier than those from ST (short-term) co-cultivation and remained healthier on LT than ST. With this approach, LT method reduced time to produce transgenic calli. Shoots were successfully regenerated from transgenic calli on SIM (Shoot Induction Medium) and rooted well on the basal medium spontaneously. The presence of antisense OMT gene was verified both by PCR and Southern analysis. Each transgenic poplar was phenotypically indishtinguishable when compared with controls for their growth pattern, leaf morphologies and xylem coloration.

• Journal of the Korea Furniture Society
• /
• v.22 no.3
• /
• pp.199-207
• /
• 2011

Examined the color of ten species of Korean broadleaf trees were experimented based on $L^*a^*b^*$ values and color difference (${\Delta}E^*ab$) between sapwood and heartwood, in order to analyze and determine the color, a critical visual and decorative factor of the broadleaf trees that can be used for making woodcraft and furniture. According to the result, the entire sapwood and heartwood, except the heartwood of Populus tomentiglandulosa, showed positive value in whiteness, redness, and yellowness and therefore contained yellow and red. None of the species showed color difference between sapwood and heartwood that was trace (0~0.5), slight (0.6~1.5), or very much; whereas Populus maximowiczii showed noticeable color difference (1.5~3.0); Salix koreensis, Betula platyphylla var japonica, Carpinus laxiflora, and Castanea crenata, appreciable and Populus tomentiglandulosa, Juglans mandshurica, Betula schmidtii, Alnus japonica, and Quercus variabilis, much. The color characteristics of heartwood and sapwood of the ten Korean broadleaf tree species studied in this research can be used as basic data for woodcraft or furniture production, especially when replacing foreign woods with Korean ones, and also contribute to convergence researches for science, engineering, and design fields.

• Journal of Korean Society of Forest Science
• /
• v.98 no.5
• /
• pp.513-523
• /
• 2009

Many species, cultivars, and hybrids in Populus have been introduced and developed by the scientists of Department of Forest Genetic Resources, Korea Forest Research Institute in Korea. P. alba ${\times}$ glandulosa, P. euramericana, P. nigra ${\times}$ maximowiczii, P. koreana ${\times}$ P. nigra var. italica, and P. davidiana have been studied from many aspects, i.e., genetics, breeding, physiology, propagation, silviculture, biomass production, biotechnology and phytoremediation. These precedent results will provide a sound basis for a newly-arising research on short-rotation coppice as one of renewable resources and phytoremediation plants. It was found that there were many promising varieties and clones for these purposes. However, minute analysis on specific gravity and caloric values for those plants should be followed under several silvicultural and rotational systems.

• Korean Journal of Environment and Ecology
• /
• v.24 no.5
• /
• pp.582-590
• /
• 2010

Baekdudaegan field survey has been proposed and presented as a various plan such as field survey analysis, management and restoration plan, etc. Whereas, the concerns of the Jeongmaek has increased continuously, nevertheless there is no research of present condition for Jeongmaek. This paper is to contribute to suggest for management and restoration as a basic study of vegetation structure in Yongso valley. We set up 30 plots($100m^2$) to verify the vegetation structure. As a result of classification analysis used by DCA, the type of 5 communities i.e. Pinus densiflora community, Betula schmidtii community, Populus maximowiczii community, Quercus mongolica community, Q. variabilis community were the most representative of Yongso valley vegetation communities. P. densiflora community was mainly distributed in the ridge of mountain, ridge of steep slope and stony area. In that reason, they avoid to compete with B. schmidtii community and Q. spp. community. In addition, the appearance of Rhododendron micranthum was mainly characteristics in the P. densiflora community. Species diversity indices(H') of groups were ranged from 0.7914~0.9942, the tree ages were 30~115 years. According to these results, the forests of Yongso valley not only form muti-layer vegetation structures but also have a great ecological value for protection and preservation.

• Journal of Korean Society of Forest Science
• /
• v.87 no.3
• /
• pp.334-340
• /
• 1998

This research was conducted to identify plant regulatory regions by gene tagging method. A promoterless GUS coding sequence was introduced to Populus nigra ${\times}$ P. maximowiczii via Agrobacterium strains(LBA4404/EHA101), and putative transgenic poplars were selected by culturing on medium containing G418($60mg/{\ell}$) and by GUS assay. Among them one positive plant was to amplify the native sequences flanking to the introduced GUS gene in plant genome by inverse PCR method and from this 730 by DNA product was obtained. After subcloning and sequencing, it has 88% homology to the Eucalyptus gunnii CAD(cinnamyl alcohol dehydrogenase) gene. The GUS gene fused with the putative promoter reinserted into poplar leaves by particle bombardment method to test the funtional promoter activity. Upon staining with X-gluc, many blue spots appeared on the leaf segments bombarded by the chimeric gene 2-3 days, thus the isolated DNA fragment contain some possible coding region as well as a putative regulatory sequences of poplar CAD gene.

• Journal of Korean Society of Forest Science
• /
• v.79 no.3
• /
• pp.278-284
• /
• 1990

We have demonstrated expression of bacterial genes transferred into cells of Populus nigra ${\times}$ P. maximowiczii by A. tumefaciens strain 6044 (pGA 472). We determined the optimum concentration of kanamycin sulfate for effective selection of punctured leaf transformed using Agrobacterium binary vector pGA 472 containing a neomycine phosphotransferase gene (NPT-II) which confers kanamycin resistance. The combination of cefotaxime (200mg/l) and carbenicillin (300mg/l) showed good performance of discarding Agrobacterium from inoculated punctured leaf. A relatively low concentration (10mg/l) of kanamycin sulfate inhibited callus and shoots induction from punctured leaf. Number of shoots regenerated from co-cultured punctured leaf was 3.0 on MS basal medium supplemented with 10 mg/l kanamycin sulfate, while that of not co-cultured punctured leaf was none. The regeneration rate was 10% from the punctured leaf co-cultured on MS medium with 10 mg/l kanamycin. Regenerated shoots are developing from micropropagation for Southern blot analysis and inheritance of the kanamycin resistance trait (NPT-II).

• Journal of the Korea Furniture Society
• /
• v.20 no.1
• /
• pp.15-20
• /
• 2009

Marvelous calligraphy-woodcarving artwork was made by using the mysterious beauty of anatomical structure of wood. The wooden artwork uses not only beautiful external appearance of wood, but it also uses the mysterious beauty of anatomical micro structure of wood which can only be observed through optical microscope or scanning electron microscope. The characteristics of anatomical structure of wood were presented through various carving techniques. The used wood species for the artwork include Quercus acutissima Carruth., Cinnamomum japonicum Sieb., Betula davurica PALL, Magnolia kobus A.P.DC, Populus maximowiczii HENRY, Betula schmidtii REGEL and Plerocaya stenoptera DC. These various types of woodcarving techniques are anticipated to be applied to the techart marketing for architecture as a field of environment art.