Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Genetic Transformation of Populus nigro x maximowiczii Using Agrobacterium tumefaciens Harboring Antisense OMT Gene

  • Ahn, In-Suk (School of Forestry and Wood Products, Michigan Technological University, USA) ;
  • Park, Young-Goo (Department of Forest, Kyungpook National University) ;
  • Shin, Dong-Ill (Department of Plant Genetic Engineering, Taegu Catholic University) ;
  • Woo, Sahng-Young (Department of Plant Genetic Engineering, Taegu Catholic University) ;
  • Park, Hee-Sung (Department of Plant Genetic Engineering, Taegu Catholic University) ;
  • Park, Jang-Won (Division of natural Resources, Taegu University) ;
  • Sul, Ill-Whan (Department of Forest Resources and Biotechnology, Daegu Polytechnoque College)
  • Published : 2001.09.01
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Abstract

An Agrobacterium tumefaciens LBA4404 (harboring antisense OMT gene)-mediated transformation method has been developed for poplar (P.nigra x maximowiczii) using prolonging co-cultivation time. Explants on LT (longterm) were induced transgenic calli one month earlier than those from ST (short-term) co-cultivation and remained healthier on LT than ST. With this approach, LT method reduced time to produce transgenic calli. Shoots were successfully regenerated from transgenic calli on SIM (Shoot Induction Medium) and rooted well on the basal medium spontaneously. The presence of antisense OMT gene was verified both by PCR and Southern analysis. Each transgenic poplar was phenotypically indishtinguishable when compared with controls for their growth pattern, leaf morphologies and xylem coloration.

Keywords

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