• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.369-375
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• 2007

We examined two Populus species (Populus euramericana and Populus alba ${\times}$ Populus glandulosa) trees grown under livestock waste leachate treatment in the field. We investigated their physiological properties (photosynthetic rate, chlorophyll contents, transpiration rate and intercellular $CO_2$ concentration) and antioxidant enzyme activities. Ascorbate peroxidase and glutathione reductase were analyzed. According to our measurements, P. euramericana at control site showed a lower total chlorophyll contents, photosynthetic rate, intercellular $CO_2$ concentration, transpiration and stomatal conductance than those of trees at treatment site. P. alba ${\times}$ P. glandulosa showed low stomatal conductance and low photosynthetic rate.

• Journal of Korean Society of Forest Science
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• v.57 no.1
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• pp.26-31
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• 1982

The hybrid poplar, populus alba ${\times}$ P.glandulosa $F_1$ shows a strong ability to produce root suckers. The relationship between crown density and root sucker was studied and the other factors that may be related to production of root sucker were also discussed. The possibility was also discussed to use its root suckers for fuelwood forests and vegetation in the area that erosion control is needed.

• Plant Resources
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• v.8 no.1
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• pp.1-8
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• 2005

A type 3 metallothionein cDNA (PMT3a) from ozone-treated Populus alba${\times}$Populus glandulosa cDNA library has been isolated and characterized. A PMT3a cDNA is 459 nucleotides long and has an open reading frame of 201 bp with a deduced amino acid sequence of 66 residues (pI 4.94). The deduced amino acid sequence of PMT3a matched to the previously reported metallothionein genes. The deduced amino acid sequence of PMT3a showed the $86\%$ identity with P. balsamifera ${\times}$P. deltoides. Expression of PMT3a by the RT-PCR was increased 60 min than 30 min after drought treatment. The ozone treated poplar increased at 30 min in the early time and then decreased at 60 min.

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.45-53
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• 1984

This experiment has been made to find out the influence of soil bulk density on growth and root development of Populus alba ${\times}$ P. glandulosa in Buyeo-kun, Chungnam. Bulk density and porosity significantly change according to slope position and soil depth and have a significant effect on tree height, DBH, biomass, and the distribution of root development. The results extracted from the experiments are as follows; 1) Bulk density in average changes from $1.17g/cm^3$ (1.05 ~ 1.40) in lower slope position to $1.43g/cm^3$ (1.36 ~ 1.60) in upper slope position, and porosity from 52.65% (55.05 ~ 45.50) in lower slope position to 41.20% (43.81 ~ 37.21) in upper slope position. 2) Bulk density increases significantly with soil depth. 3) Tree height, DBH, and total biomass decrease with bulk density. 4) Foliage, branch, bolewood, bolebark, and root also significantly have a negative correlation with hulk density. 5) Populus alba ${\times}$ P. glandulosa has a shallow root system chiefly distributing only in AI horizon, and critical bulk density is $1.43g/cm^3$. Therefore, slope position, hulk density, and porosity are considered to influence on the growth, biomass, and root development of Populus alba ${\times}$ P. glandulosa.

• Plant Resources
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• v.8 no.1
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• pp.52-59
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• 2005

A cDNA, PSOD1, encoding cytosolic copper/zinc superoxide dismutase (CuZn-SOD) was cloned and characterized from a full length cDNA library prepared from Populus alba${\times}$Populus glandulosa cultured in vitro. A PSOD1, is 725 nucleotides long and has an open reading frame of 459 bp with 152 amino acid residues (pI 5.43). The deduced amino acid sequence of PSOD1 perfect matched to the previously reported CuZn-SOD (CAC33845.1). Consensus amino acid residues (His-45, -47, -62, -70, -79, -119) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of PSOD1 exhibited the high level of similarity from 100 to $85\%$ among previously registered SOD genes. The expression of PSOD1 in poplar increased at the 1 mM $H_{2}O_2$ and drought stress during 30 min and 60 min, but the ozone treated poplar increased at 30 min in the early time and then decreased at 60 min.

• Journal of Korean Society of Forest Science
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• v.49 no.1
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• pp.11-31
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• 1980

The morphological, anatomical and physiological traits were eximined for Populus alba ${\times}$ glandulosa which is an important planting species in Korea. The results obtained are as follows: 1. External characters in the leaf shape and chaff shape in the catkin were inherited as incomplete dominance but nectar gland was inherited as dominance. 2. Among the 15 selected clones, 9 clones were male, 2 clones female and 2 clones monoecious. 3. There were well-developed cork layers and bast fiber bundles in the bark. 4. Primordial leaves composed of 3 layers of cells and those undifferentiated into palisade and spongy parenchymas differed in its origin. 5. Leaf scare consisted of two kinds of tissues; one is connected to vascular bundle and the other not to vascular bundle. Tissues which had been connected to vascular bundle were isolated with only 2 or 3 layers of cork cells from the outside. 6. There was complicated arrangement in the vascular bundle of petioles. 7. Growth of the hybrid was sensitively influenced by external temperature, day-length and amount of light. In particular, it was apparent in height growth. 8. Flatness, loam soils and a $60{\times}60cm$ spacing might be best factors for the growth of P. alba ${\times}$ glandulosa. 9. The rooting of 15 clones was dependant upon external factors. 10. The growth of P. alba ${\times}$ glandulosa was best at around 80% of soil moisture content on the basis of plot water capacity. 11. Temperature difference between inside and outside stems below 100cm during the winter was the greatest at the south among seasons and among directions. 12. The sap movement was markedly influenced by air temperature, relative humidity in forest stand and moisture content in stem. 13. Total sugars in the cortex changed with season but did not differ in the dircetion of the stem. 14. Isoperoxidase variations in the leaf were different among 15 clones. Thus, it may be useful as a criterium for clonal identification. 15. The rate of soil moisture content decreased at a rapid slope was faster than that at a slow slope. Poor growth of P. alba ${\times}$ glandulosa at the slope was probably due to depletion of soil moisture.

• Journal of Korean Society of Forest Science
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• v.43 no.1
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• pp.1-5
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• 1979

The arrangement and the number of vascular bundle in petiole were investigated with the hybrid Populus alba${\times}$P. glandulosa and both parents. 1. The variation in number and the arrangement of the vascular bundle in petiole were smaller at middle point than at nearer point to the leaf blade. 2. A small variation was found in the arrangement of the vascular bundle within a tree, same clone and same species. 3. Five shapes of vascular bundle were recognized in the $F_1$ hybrid, 26.7 per cent of the $F_1$ hybrid has the same shape with P. alba, 13.3 percent with P. glandulosa and 53.3 per cent of the $F_1$ hybrid shows the $F_1$ shape caused by hybridization. 4. The hybrid clones which show the same shape with P. alba are 66-20-1, 66-6-8, 65-22-11 and 64-6-44, hybrid clones of 65-95, 66-14-93 have the same shape with P. glandulosa. Hybrid clones of 66-15-3, 67-6-3, 65-22-4, 66-26-55, 68-1-54, 66-14-99, 65-29-19, 66-25-5 have $F_1$ shape.

• Journal of Korean Society of Forest Science
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• v.45 no.1
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• pp.1-10
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• 1979

This study was conducted to widen the range of characteristics of the hybrids, P.alba ${\times}$ P.glandulosa, i.e. aiming for gene population expansion P.glandulosa seemed to have the similar characteristics as the one segregated from the natural hybrids between P.alba and P.davidiana. Thus the main objectives of this study were to make many crosses among poplars and then to identify leaf characteristics of the crosses similar to P.glandulosa, the results obtained can be summerized as follows; 1. Leaf characteristics such as leaf margin, presence of glands at leaf base and pubescence density, of crosses made from P.alba.davidiana ${\times}$ P.datidiana, and P.davidiana.alba ${\times}$ P.davidina showed 44% and 90%, respectively, of similarity to P.glandulosa. 2. The ratio of leaf size, including leaf length, leaf width, length from leaf base to width line, and petiole length, of the above crosses was similar to P.glandulosa. 3. Pubescence density of the dorsal leaf surface in hybrids between P.alba and P.davidiana showed generally intermediate of the parental appearance. Frequency of pubescence appearance differed from depending upon the use of P.alba, either as a female or a male parent. The use of P.alba as a male parent increased frequency of Pubescence appearance. 4. The presence of glands at the leaf base in P.glandulosa may be inherited from P.davidiana which possesses gland although gland is not present in all P.davidiana rather from P.alba which has no gland.

• Journal of the Korean Wood Science and Technology
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• v.37 no.1
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• pp.114-120
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• 2009

The bark of Populus alba ${\times}$ glandulosa was collected, air-dried and extracted with 70% aqueous acetone. Then it was successively partitioned with n-hexane, $CH_2Cl_2$, EtOAc and $H_2O$. Repeated Sephadex LH-20 column chromatography and preparative TLC on the EtOAc soluble fraction gave a grandidentatin isomer. The structure was elucidated as grandidentatin A (cis-2-hydroxycyclohexyl 6-O-p-coumaroyl-${\beta}$-D-glucopyranoside) on the basis of spectroscopic evidences such as $^1H$-NMR, $^{13}C$-NMR, 2D-NMR and MALDI TOF-MS spectrum followed by acid hydrolysis. Grandidentatin A was identified here for the first time in Populus alba ${\times}$ glandulosa bark, and to the bset of our knowledge it has not been reported in any other literature.

• Journal of Ecology and Environment
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• v.34 no.4
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• pp.393-400
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• 2011

The effects of the ectomycorrhizal fungus Pisolithus tinctorius and cadmium (Cd) on physiological properties and Cd uptake by Populus alba ${\times}$ glandulosa was investigated under greenhouse conditions. Cd treatment decreased the photosynthetic rate ($P_N$) of both non-mycorrhizal (NM) plants (16.3%) and ectomycorrhizal (ECM) plants (11.5%). In addition, the reduction in total dry weight by Cd treatment was greater in ECM plants (24.3%) than that in NM plants (17.6%). Mycorrhizal infection increased the $P_N$ and transpiration rate in both control and Cd-treated plants. Cd treatment increased superoxide dismutase (SOD) activity and decreased glutathione reductase activity, and the increase of SOD activity by Cd treatment was greater in NM plants (40.3%) than that in ECM plants (3.7%). Thiol content increased in both NM and ECM plants treated with Cd solution, and the increase in thiol content in NM plants (43.9%) was greater than that of ECM plants (15.6%). Cd uptake in the leaves, stems, and roots of ECM plants was 69.9%, 167.2% and 72.8%, respectively, higher than in the NM plants. However, the increase in Cd uptake ability of ECM plants resulted in a reduction in dry weight.