• 생태와환경
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• 제38권spc호
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• pp.44-53
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• 2005

The Nakdong River, which lies in a monsoon climate zone with warm rainy summers and cold dry winters, is a typical ecosystem showing the attributes of a regulated river. In 2003, the total annual rainfall (1,805 mm) was higher than the average of the past nine years from 1994 to 2002 (1,250 mm). In September a powerful typhoon, Maemi, caused a big impact on the limnology of the river for over two months. Among the limnological variables, turbidity in 2003 (37.4 ${\pm}$ 94.1 NTU, n = 54) was higher than the annual average for ten years (18.5 ${\pm}$ 2.3 NTU, n = 486) in the lower part of the river (Mulgum: RK 28). Furthermore, physical disturbance (e.g. stream bank erosion within channel) in the upstream of the Imha Dam (RK ca. 350; river distance in kilometer from the estuary barrage) in the upper part of the river was a source of high turbidity, and impacted on the limnological dynamics along a 350 km section of the middle to lower part of the river. After the typhoon, high turbidity persisted more than two months in the late autumn from September to November in 2003. Flow regulation and the extended duration of turbid water are superimposed on the template of existing main channel hydroecology, which may cause spatial changes in the population dynamics of plankton in the river.

• 수면정신생리
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• 제23권1호
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• pp.25-28
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• 2016

목 적 : Retinoid-related orphan receptor A (RORA) 유전자가 일주기리듬의 조절에 영향을 미친다는 사실이 알려져 왔다. 본 연구에서는 RORA 유전자다형성과 일중선호도의 연관성을 한국인 젊은 성인을 대상으로 분석하였다. 방 법 : 광고를 통해 모집한 504명의 젊은 성인이 최종적으로 대상에 포함되었다. 모든 대상은 13문항 composite scale for morningness (CSM)를 작성하였다. RORA rs11071547 단일염기다형성은 PCR 기반한 실험 방법을 통하여 분석하였다. 결 과 : CSM 점수결과 일중선호도는 RORA rs11071547 SNP의 유전자형과 대립유전자 보유여부에 따라 차이를 보이지 않았다. 결 론 : 이러한 연구결과는 RORA rs11071547 SNP가 일중선호도에 중요한 역할을 하지 않을 가능성을 보여준다.

• 한국환경농학회지
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• 제22권2호
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• pp.137-147
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• 2003

국내에서 발생되는 하수오니에 대한 퇴비원료로의 활용성을 검토하기 위하여 전국 150개소의 하수처리장 중에서 82개소로부터 88점의 하수오니를 채취하여 중금속 함량을 분석하였다. 하수오니의 구분은 하수처리장이 위치한 지역의 인구를 기준으로 광역시(100만명 이상), 대도시(50만${\sim}$100만명), 중소도시(10만${\sim}$50만명) 및 농촌지역(인구 10만명 이하 소도시 포함)으로 구분하여 몇 가지 중금속을 분석하고 현재의 퇴비원료 기준과 비교 검토하였다. 성분별 전국적인 분포는 As $0.0{\sim}54.3\;mg/kg$, Cd $1.7{\sim}197.3\;mg/kg$, Cr $7.3{\sim}2,854\;mg/kg$, Cu $87.4{\sim}5,730\;mg/kg$, Mn $129.3{\sim}10,289\;mg/kg$, Ni $20.1{\sim}2,057\;mg/kg$, Pb $7.6{\sim}197.5\;mg/kg$, Zn $523.5{\sim}6,349\;mg/kg$의 범위에 있었다. 퇴비원료기준과 비교하였을 때 As, Cd, Cr, Cu, Ni, Pb 및 Zn 중 어느 한 성분이라도 함량이 초과하는 비율은 광역시 및 대도시는 100%, 중소도시 95%, 농촌지역 93%로 나타났다. 중금속 성분별로는 전체시료 중 As는 1%, Cd 41%, Cr 15%, Cu 53%, Ni 47%, Pb 2% 및 Zn은 89%가 기준치를 초과하였다.

• Journal of Ginseng Research
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• 제40권1호
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• pp.18-27
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• 2016

Background: It is not clear whether ginseng affects cyclosporine A (CsA)-induced desirable immunosuppressive action. In this study, we evaluated the immunological influence of combined treatment of ginseng with CsA. Methods: Using CD4+ T cells from mouse spleens stimulated with the T cell receptor (TCR) or allogeneic antigen-presenting cells (APCs), we examined the differentiation of naïve T cells into T helper 1 (Th1), Th2, Th17, and regulatory T cells (Tregs), and their cytokine production during treatment by Korean Red Ginseng extract (KRGE) and/or CsA. The influence of KRGE on the allogeneic T cell response was evaluated by mixed lymphocyte reaction (MLR). We also evaluated whether signal transducer and activator of transcription 3 (STAT3) and STAT5 are implicated in this regulation. Results: Under TCR stimulation, KRGE treatment did not affect the population of CD4+interferon gamma ($IFN{\gamma}$)+ and CD4+interleukin (IL)-4+ cells and their cytokine production compared with CsA alone. Under the Th17-polarizing condition, KRGE significantly reduced the number of CD4+IL-17+ cells and CD4+/phosphorylated STAT3 (p-STAT3)+ cells, but increased the number of CD4+CD25+forkhead box P3 (Foxp3)+ cells and CD4+/p-STAT5+ cells compared with CsA alone. In allogeneic APCs-stimulated CD4+ T cells, KRGE significantly decreased total allogeneic T cell proliferation. Consistent with the effects of TCR stimulation, KRGE reduced the number of CD4+IL-17+ cells and increased the number of CD4+CD25+Foxp3+ cells under the Th17-polarizing condition. Conclusion: KRGE has immunological benefits through the reciprocal regulation of Th17 and Treg cells during CsA-induced immunosuppression.

• 인간행동과 음악연구
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• 제17권2호
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• pp.29-56
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• 2020

본 연구의 목적은 난임 여성의 정서조절기술 향상을 위한 심상 중심 음악감상 프로그램을 개발하고 타당도를 검증하는 것이다. 이를 위해 먼저 난임 여성의 정서적 어려움과 이에 대한 대처방식과 관련된 선행연구를 조사하여 난임 여성의 정서적 필요에 개입할 수 있는 이론적 기반을 확립하였다. 또한, 지지적 음악심상기법(Supportive Music and Imagery, 이하 SMI) 관련 선행연구를 고찰하여 중재의 핵심 요소들을 분석했다. 확인된 이론적 기반 및 도출된 핵심 요소를 토대로 본 프로그램이 개발되었고, 음악과 심상 전문가 4인에게 타당도 검증을 받은 후 수정 및 보완되었다. 연구 결과, 개발된 본 프로그램은 긍정 정서 자원의 발견-강화-확립의 체계적 구성을 통해 난임 여성의 긍정적 정서 변화에 효과적으로 적용될 수 있는 것으로 확인되었으며 이후 긍정 정서 자원을 규명하고 확립하는 데 사용할 수 있도록 정서유도 및 조절과 관련하여 기능할 수 있는 음악요소의 특성이 분석된 음악 자원 역시 제시되었다. 본 프로그램은 각 개인이 심상 중심 음악감상을 통해 내적자원을 발견하도록 지지함으로써 난임으로 인한 부적 정서에 대처하고 긍정 정서 자원을 확립할 수 있도록 하는 데 초점을 맞춘 프로그램이라 할 수 있다. 또한, 난임 여성을 대상으로는 처음으로 적용된 본 프로그램은 기존의 SMI를 특정 대상군의 필요에 맞게 응용·확장한 것으로 기존의 일반적 SMI 적용에서 한발 나아가 새로운 시도를 한 것에 학술적 의의가 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.941-946
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• 2008

Single-minded 1 gene (SIM1) is a homolog of Drosophila SIM1 gene which plays a key role in the midline cell lineage of the central nervous system and is implicated in the regulation of feeding behavior and obesity in the human and mouse. In this study, porcine SIM1 gene was firstly mapped to chromosome 1p13 using radiation hybrid (RH) mapping and two polymorphisms were detected at position 607 (A/G) in SIM1 intron7 and position 780 (C/T) in SIM1 exon8. The last substitution was genotyped in a 364 F2 animal-population and an association analysis of these genotypes was performed with growth, carcass and meat quality traits by the statistical animal model. The results showed the significant influence of the SIM1 genotype on growth (p<0.05): live weight at birth, later period of growth and average daily gain; and effects on carcass composition (p<0.05): weight of head and buck kneed foreleg, backfat depth, loin eye area, carcass leaf fat and ham fat weights; and traits related to intramuscular fat content (p<0.05).

• 동국한의학연구소논문집
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• 제11권
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• pp.41-51
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• 2008

Dietary supplements are used by more than one-half of the adult US population. By contrast, herbal products in Germany are carefully regulated by the same standards as drugs, and efforts are under way to standardize their regulation in the entire European Union. Most herbal users do not inform their physicians that they are taking these supplements, and most physicians do not inquire. Although some herbal products have clinically proven benefits, it is increasingly apparent that many contain potentially toxic substances, particularly in relation to interactions with drugs. Hence, it is essential that practicing physicians develop a working knowledge of herbals-specifically, about claims for their usage and potential or proven efficacies and toxicities-and that they incorporate such knowledge into the evaluation and management of their patients. By contrast, functional foods-integral components of the diet that are understood to contribute added health benefits-are the subject of intense and widespread research in food and nutritional science. Examples include many polyphenolic substances, carotenoids, soy isoflavones, fish oils, and components of nuts that possess antioxidant and other properties that decrease the risk of vascular diseases and cancer. Practicing physicians are advised to stay abreast of these emerging findings in order to best advise their patients on the value of health-promoting diets in disease prevention.

• 한국환경성돌연변이발암원학회지
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• 제24권3호
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• pp.113-120
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• 2004

Chromium compounds are known human and animal carcinogens. In this study, the effects of sodium chromate on apoptosis and cell cycle were investigated in order to unveil the elements of early cellular responses to the metal. Using Chinese hamster ovary cells(CHO-K1-BH4), we found taht chromium (VI) treatment induced apoptosis in these cells, as signified by nuclear fragmentation, DNA laddering on agarose gel electrophoresis, and an increased proportionof cells with hypodiploid DNA. Preceding these changes, chromium (VI) treatment increased caspase 3 pritease activity and also increased expression of p53 protein, while the level of bcl2 protein was not changed. Coincubation with caspase inhibitor, Z-DEVD-FMK, inhibited chromium-induced apoptosis. In the flow cytometric analysis using propidium iodide fluorescence, an increase of cell population in G2/M phase was shown in cells exposed to at least 160 $\mu\textrm{m}$ of sodium chromate for 72h, form 9.8% for 0$\mu\textrm{m}$ chromium (VI) to 26.4% for 320$\mu\textrm{m}$ chromium(VI). Taken together, these findings suggest that chromium(VI)-induced apoptosis is accompanied by G2/M cell cycle arrest, and that p53-mediated pathway may be involved in positive regulation of G2/M arrest and a concurred apoptosis in CHO cells.

• Journal of Animal Science and Technology
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• 제56권8호
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• pp.27.1-27.6
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• 2014

The fat mass and obesity associated (FTO) gene plays an important role in the regulation of energy homeostasis, fat deposition and obesity. For this reason, the FTO gene is a physiological and functional candidate gene for carcass and meat quality traits in beef cattle. The objectives of this study were to identify SNPs in the exonic regions of FTO gene and to evaluate the association of these SNPs with carcass traits in Hanwoo (Korean cattle). In this study, we newly identified two exonic SNPs in Hanwoo population. The g.125550A > T SNP was located in exon 3 and the g.175675C > T SNP was located in exon 6. Genotyping of the two SNP markers was carried out using PCR-RFLP analysis in Hanwoo steers to evaluate their association with carcass traits. As a result, g.125550A > T SNP genotype was significantly associated with effects on marbling score. Animals with the AA and TT homozygous genotypes had a significantly higher marbling score (p < 0.001) than those with AT heterozygous genotype, and this was significant after Bonferroni correction of the significance threshold (p = 0.003). Dominance effect was also observed for the marbling score (P < 0.05) with higher marbling score of homozygous animals. However, no significant associations with meat quality traits were observed for the g.175675C > T SNP. Our results suggest that the exonic SNP g.125550A > T in the FTO gene may be used as a DNA marker for the selection of Hanwoo with higher marbling.

• 한국식품위생안전성학회지
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• 제10권2호
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• pp.81-87
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• 1995

Daily exposure of aflatoxin B1 (AFB1) was estimated in foods (rice, barley, soybean, peanut, soysauce, soybean paste) by ELISA (enzyme linked immunosorbent assay) using polyclonal antibody R101. Before ELISA, a simple extraction method was applied for the quantitation of AFB1 in foods using chloroform which showed high recovery (70$\pm$12%). AFB1 levels in foods were 0.32 ng/ml (rice), 0.24ng/ml (barley), 0.22 ng/ml (peanut), 0.30~0.78 ng/ml (soysauce), and 0.2 ng/ml (soybean paste). Based on food consumption, we estimated that Koreans were exposed to AFB1 at the level of 1.86$\pm$0.46 ng/kg/day and liver cancer incidence attributed to AFB1 exposure (assuming that AFB1 as a single hepatocarcinogenic agent) might be calculated to be 13.1 per 100, 000 population. Our data demonstrate that AFB1 levels in foods were below the regulation of 10 ppb in foods and might not be the major risk factor for the high incidence of lover cancer in Korea.