• 한국조리학회지
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• 제15권1호
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• pp.120-127
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• 2009

석류씨, 석류 외피 분말 추출물 및 석류 과즙(주스 1과 주스 2) 추출물을 DPPH 라디컬 소거활동에 HepG-2 세포 그리고 항산화 활동에 추출물의 성장억제를 측정하였다. 높은 농도를 가진 모든 샘플은 HepG-2 세포에 대하여 성장 억제를 보였으며, 2,500 ppm의 처리구에서는 2회 감압 농축한 과즙 농축물(43%)>석류씨 분말 추출물(42%)>석류외피 분말 추출물(38%)>1회 감압 농축한 과즙 농축물(29%) 순으로 나타났다. BHT와 시료들 모두 농도 의존적으로 DPPH 라티칼 소거 활성이 증가하는 경향을 보였고, 12.5 ppm의 처리구에서 석류외피 분말 추출물(60.8%)>BHT(29.9%)>석류씨 분말 추출물(16.2%)>2회 감압 농축한 과즙 농축물(15.1%)>1회 감압 농축한 과즙 농축물(12.6%)의 순으로 나타났으며, 25, 50, 100 및 200 ppm처리구에서는 석류외피 분말 추출물(81.9$\sim$85.3%)>석류씨 분말 추출물(33.4$\sim$83.0%)>BHT(31.3$\sim$47.8%)>2회 감압 농축한 과즙 농축물(15.4$\sim$36.8%)>1회 갑압 농축한 과즙 농축물(13.4$\sim$36.1 %)의 순으로 나타났다.

• 한국식품저장유통학회지
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• 제16권5호
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• pp.726-733
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• 2009

본 연구에서는 6종류의 종실에서 기름을 착유하여 조지방 함량(crude fat content) 측정 및 총 지방산 조성과 위치별 지방산 조성을 비교 분석 하였으며, triacylglycerol(TAG)의 조성과 tocopherol 함량을 분석하였다. Folch법을 이용하여 분석된 조지방 함량은 들깨 21.64%, 홍화씨 13.85%, 고추씨 9.60%, 석류씨 8.85%, 녹두 2.25%, 결명자 2.00%로 나타났다. 추출된 종실유의 지방산 분석결과 불포화 지방산인 linoleic acid(C18:2)가 81.57 wt%~46.17 wt%로 가장 높은 비중을 차지하였으며, sn-2 위치의 지방산 조성에서도 linoleic acid의 함량이 88.30 wt%~15.99 wt%로 높은 함량을 나타내었다. Triacylglycerol(TAG)의 조성은 reversed-phase HPLC를 이용하여 분석하였으며 partition number(PN)=36~48의 분포를 보였다. Total tocopherol의 함량은 석류씨에서 가장 높은 377.74 mg /100 g으로 나타났으며 이 외에 각각 녹두(141.16 mg/100 g), 결명자(107.23 mg/100 g), 고추씨(33.88 mg/100 g), 들깨(30.05 mg/100 g), 홍화씨(29.80 mg/100 g) 순으로 측정되었다.

• 한국미생물·생명공학회지
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• 제43권4호
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• pp.357-366
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• 2015

쿼럼센싱은 세포 간의 의사소통 방법이며 박테리아의 병원성과 관련된 유전자들의 조절메커니즘이다. 박테리아는 다양한 생리학적 과정들을 제어하기 위해 이 쿼럼센싱 시스템을 활용한다. 본 연구에서 석류(Punica granatum L.) 껍질 추출물이 바이오 리포터 균주인 Chromobacterium violaceum 과 C. violaceum CV026에서 쿼럼센싱 억제능을 갖는 것으로 1차 선별되어 식중독균인 Y. enterocolitica에서 편모에 의한 운동능과 바이오필름형성 억제능에 대한 석류껍질 추출물의 효과에 대한 다음 실험을 수행하였다. 추가로 N-acylhomoserine lactones (AHLs)의 합성(yenI and yenR)과 편모 레귤론(fliA, fleB and flhDC) 에 관련된 특정유전자의 발현변화를 역전사 중합효소연쇄반응법으로 평가하였다. 결과는 석류껍질 추출물이 C. violaceum CV026에서 쿼럼센싱으로 제어되는 바이오레신 생산을 78.5%까지 억제하였으며, Y. enterocolitica에서는 세포의 성장에 영향을 주지 않고 바이오필름 형성과 편모 운동성을 현저히 감소시키는 것을 확인할 수 있었다. 이러한 억제 효과는 AHLs의 합성과 운동성에 관여하는 유전자 발현을 down-regulation 하는 결과와도 일치하였다. 본 연구의 결과는 석류껍질 추출물의 임상 적용을 위하여 생체 내 특성에 대한 추가적인 연구가 필요하다는 것뿐 아니라 석류껍질 추출물이 사람의 위장관염을 방지하기 위한 잠재적인 치료제가 될 수 있다는 것을 보여준다.

• 한국식품영양과학회지
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• 제44권1호
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• pp.160-164
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• 2015

석류 농축액의 첨가량을 0~6.6%로 달리하여 백설기를 제조한 후 물리화학적 품질, 항산화 특성 및 소비자 기호도를 비교하였다. pH는 6.35~5.61 범위로 석류 농축액의 첨가량이 증가할수록 유의적으로 감소하였고(P<0.05) 수분함량 또한 유의적으로 감소하였다(P<0.05). 한편 명도($L^*$)는 농축액의 첨가량이 많아질수록 유의적으로 감소하였지만 (P<0.05) 적색도($a^*$)와 황색도($b^*$)는 석류 농축액의 첨가량이 많아질수록 유의적으로 증가하였다(P<0.05). 경도는 0.18~0.34 kgf로 대조군과 1.6%, 3.3~5% 첨가군 내에서 유의적 차이는 발견되지 않았으나(P>0.05) 전반적으로 증가하는 경향을 나타내었으며, 전자공여능과 ABTS radical 소거능 활성은 석류 농축액의 첨가량이 증가할수록 유의적으로 증가하였다(P<0.05). 소비자 검사 결과 농축액을 첨가한 백설기가 그렇지 않은 시료에 비해 대체적으로 낮은 평가를 받았으나 전반적인 소비자 기호도와 석류 농축액의 건강기능성 효과 등을 고려하면 최적 첨가 농도는 1.6%가 적절한 것으로 판단된다.

• 동의생리병리학회지
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• 제33권2호
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• pp.131-140
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• 2019

The objective of present study is to evaluate concentration-dependent in vitro anti-osteoarthritic (OA) effects of synergic mixed formula consisted of dried pomegranate juice concentrate powder, Eucommiae Cortex aqueous extract and Achyranthis Radix aqueous extract 5:4:1 (g/g) mixture on the primary cultured rat articular chondrocytes. First, any cytotoxic effect of mixture was observed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium Bromide) assay. Next, cyto-protective effect of test substances was evaluated by using the recombinant human interleukin $(rhIL)-1{\alpha}$ induced chondrocytes. In addition, anti-inflammatory effects were also observed on the lipopolysaccaride (LPS) treated chondrocytes through prostaglandin $E_2(PGE_2)$ productions and 5-lipoxygenase (LPO) activities, and inhibitory effects on matrix metalloproteinase (MMP)-2 and MMP-9 activities were observed on $rhIL-1{\alpha}$ treated chondrocytes with their extracellular matrix (ECM) related mRNA expressions. No obvious cytotoxic effects of mixture were demonstrated. Inflammatory damages of chondrocytes and related ECM degradations induced by treatment of LPS or $rhIL-1{\alpha}$ were significantly and concentration-dependently inhibited by pretreatment of mixture from a concentration level of 0.001 mg/ml to 1 mg/ml. In addition, mixture showed $IC_{50}$ for $rhIL-1{\alpha}-induced$ MMP-2 and MMP-9 activities as 44.01 and $162.47{\mu}g/ml$, and also showed $EC_{50}$ for $rhIL-1{\alpha}-induced$ inhibition of collagen type II, SOX9 and aggrecan mRNA expression as 8.61, 10.79 and $4.47{\mu}g/ml$, respectively. It is observed that mixture showed concentration-dependent anti-inflammatory and cytoprotective ECM preserved effects on the primary cultured rat articular chondrocytes without cytotoxicity.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1161-1171
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• 2019

Objective: The current study aimed to replace soybean oil in broiler diets with linseed oil, which is rich in omega-3 fatty acid supplemented with pomegranate peel extract (PPE) and measured its effect on broiler performance, carcass traits, lipid profile, as well as fatty acids composition, phenols and flavonoids content of broiler muscles and immunity of broiler chicks. Methods: A total of 300 1-day-old Cobb chicks were randomly allotted into six experimental groups, T1 fed on basal diet with soybean oil without any additives, T2 fed on basal diet with soybean oil with addition of 0.5 g/kg diet PPE, T3 fed on fed on basal diet with soybean oil with addition of 1 g/kg diet PPE, T4 fed on basal diet with linseed oil without any additives, T5 fed on basal diet with linseed oil with addition of 0.5 g/kg diet PPE and T6 fed on basal diet with linseed oil with addition of 1 g/kg diet PPE. The PPE supplementation with 0.05% improved final body weight with either soybean oil ration or linseed oil ration. Results: The PPE improved carcass dressing percentage in comparison with the control groups. Body fat levels decreased with increasing PPE levels, especially with a linseed oil diet. Replacing soybean oil with linseed oil decreased the total cholesterol and triacylglycerol levels in broiler serum. The PPE supplementation decreased serum total cholesterol levels and increased high-density lipoprotein cholesterol levels. The content of the breast muscle alpha linolenic acid improved after replacement of soybean oil with linseed oil in broiler diets. PPE supplementation increased the phenol and flavonoid content in broiler meat and increased lysozyme activity. Conclusion: Replacing soybean oil with linseed oil in broiler diets with the addition of PPE enriched muscle meat with omega-3 fatty acids and antioxidants and improved broiler immunity and their serum lipid profile.

• 한국융합학회논문지
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• 제12권12호
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• pp.153-159
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• 2021

본 연구의 목적은 시판되고 있는 청포도 주스와 석류 주스의 노출 시간에 따른 치아 표면 변화에 대한 위험성을 융복합적으로 확인하고자 한다. 발치된 건전 치아를 사용하였으며, 접촉시간에 따른 구분으로 총 8그룹으로 대조군과 10 ml의 시판되는 청포도 주스와 석류 주스를 1, 3, 5, 15, 30, 60, 120분 적용한 실험군 7그룹으로 나누었다. 실험 주스의 pH가 측정되었고 표면의 변화와 미세 형태는 주사전자현미경(Scanning Electron Microscope:SEM)을 통하여 확인하였다. 본 실험에 사용된 청포도 주스는 3.9±0.02, 석류 주스는 3.5±0.01로 제조사가 명시한 주스의 pH 범위보다 청포도 주스는 약간 높았지만 석류 주스는 낮았다. SEM을 통한 결과에서도 대조군과 비교하여 치아 표면의 손상이 생겼다. 접촉 시간이 증가될수록 표면의 손상은 증가되었다. 연구의 결과를 바탕으로 음용 횟수나 구강 내 저류 시간을 줄이고, 치과 임상에서 치아 침식증 환자에게 질환의 진행을 낮출 수 있는 과일 주스에 대한 정확한 정보를 제공해야 할 것이다.

• 한국축산식품학회지
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• 제43권1호
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• pp.124-138
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• 2023

This study was carried out to determine the effects of adding pistachio shell (PIS), pomegranate hull (POM), and olive pulp (OP) to the diet on milk amino acid and fatty acid parameters in Awassi sheep. In the study, 40 head of Awassi sheep, which gave birth at least twice, were used as animal material. Sheep were fed a control diet without added byproducts (CON), rations containing PIS, POM, and OP. Milk amino acid profile was determined by liquid chromatography-tandem mass spectrometry, milk fatty acid gas chromatography-flame ionization detection device. There was a dramatic reduction in alanine, citrulline, glutamine, glutamic acid, glycine, leucine, ornithine and alphaaminoadipic acid in the research groups. In the PIS group, argininosuccinic acid, gammaminobutyric acid, beta-alanine and sarcosine; In the POM group, asparagine, gammaminobutyric acid, beta-alanine, and taurine; In the OP group, a significant positive increase was found in terms of alanine, histidine, gammaminobutyric acid, and taurine amino acids. The applications in the study did not have a statistically significant effect on the ratio of short, medium and long chain fatty acids in milk (p>0.05). In the presented study, it was determined that PIS, POM, and OP, which were added to the sheep rations at a rate of 5%, caused significant changes in the milk amino acid profiles. In this change in milk amino acid profiles, the benefit-harm relationship should be considered.

• 대한약침학회지
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• 제26권3호
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• pp.265-275
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• 2023

Objectives: The aim of this study was to evaluate the effect of fruit juices on Multi-Drug Resistant (MDR) bacterial pathogens involved in Urinary Tract Infections (UTIs) and Sexually Transmitted Infections (STIs) among tribal women in the district Anuppur, Madhya Pradesh, India. Methods: Fresh juices of lemon (Citrus limon), amla/Indian gooseberry (Phyllanthus emblica), pineapple (Ananas comosus), mosambi/sweet lime (Citrus limetta), orange (Citrus sinensis), kiwi (Actinidia deliciosa), and pomegranate (Punica granatum) fruits were evaluated for in vitro antibacterial activity against bacterial pathogens involved in UITs and STIs among tribal women. Physico-chemical analysis of fresh fruits was also carried out by measuring the pH, moisture, protein, fat, crude fibre, carbohydrate, and ascorbic acid content. Results: Lemon and amla juice showed better antibacterial activity against the pathogens as compared to other juices. MIC results fruit juices against UTIs and STIs pathogens vary depending on the specific pathogen and juice chemical constituents. The physico-chemical analysis showed that the moisture content was highest in mosambi (90%), followed by orange (87%). Ascorbic acid content was found highest in amla (540 mg/100 g), followed by kiwi (90.3 mg/100 g). Pomegranate showed highest concentration of carbohydrate (15.28 g/100 g), fat (1.28 g/100 g), and protein (1.65 g/100 g). Lemon juice had lowest pH of 2.20, followed by amla 2.67. Conclusion: The lemon juice showed highest antibacterial activity against MDR bacterial pathogens involved in UTIs and STIs among tribal women in district Anuppur, Madhya Pradesh, India. The low pH of lemon may be responsible for its high antibacterial activity as compared to other juices.

• 한국의류산업학회지
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• 제4권2호
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• pp.117-127
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• 2002

Patterns presented at baroque were mostly floral pattern. Abstract anonymous flower, tulip, Acandus leaf, pomegranate were blended with one another and had a distinct leaning toward continuation and repetition. Components of design could be classified into flower and leaf, flower and fruit, flower and circumferential ornament, bunch of flowers, flower and pot, flower and geometry, and flower and animal. Floral patterns in this era were mostly fullness type being large, simple, and having more formalized motive and were fronted with unit type and vine shape. Unit type shows reflection of baroque style which aims to present absolute unification rather than freedom, which was inherent property of baroque style, by using symmetric pattern. Vine type were unfolded with C type curved line which was main characteristics of that time and showed flowage as a whole and pliable beauty. Floral pattern was filled the whole surface of fabric, thus background of fabric can not be discerned. So, it showed extreme decorative tendency and lavishness.