Culinary science and hospitality research (한국조리학회지)

Culinary Society of Korean Academy (한국조리학회)
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The Effects of Pomegranate Extracts on the Growth Inhibition against HepG-2 Liver Cancer Cells and Antioxidant Activities

석류 추출물의 간암세포 성장 억제 및 항산화 활성 효과

  • Park, Kyong-Tae (Dept. of Hotel Culinary Arts & Nutrition, Kaya University) ;
  • Kim, Du-Woon (Dept. of Nutrition and Food, Chonnam National University) ;
  • Sin, Tae-Sun (Dept. of Nutrition and Food, Chonnam National University) ;
  • Shim, Sun-Yup (Institute of Marine Life Science, Pukyong National University) ;
  • Kim, Mun-Yong (Dept. of Food and Nutrition, Sunchon National University) ;
  • Chun, Soon-Sil (Dept. of Food and Nutrition, Sunchon National University)
  • 박경태 (가야대학교 호텔조리영양학과) ;
  • 김두운 (전남대학교 영양식품학과) ;
  • 신태선 (전남대학교 영양식품학과) ;
  • 심선엽 (부경대학교 해양생명과학연구소) ;
  • 김문용 (순천대학교 식품영양학과) ;
  • 전순실 (순천대학교 식품영양학과)
  • Published : 2009.03.30
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Abstract

In this study, Pip(Pip) and shell powder(Cortex) extracts, and juice concentrates(Juice-1 and Juice-2) of Pomegranate were screened for their growth inhibition on HepG-2 cells and antioxidant activities on DPPH radical-scavenging activity. All samples with higher concentrations showed the growth inhibition against HepG-2 cells. The growth inhibitions against HepG-2 liver cancer cells at 2,500 ppm were in order of Juice-2(43%), Pip(42%), Cortex(38%) and Juice-1(29%). DPPH radical-scavenging activities were higher with the concentrations of the samples increased. The activities of antioxidant BHT, Pip, Cortex, Juice-1, and Juice-2 at 12.5 ppm were 29.9, 16.2, 60.8, 12.6, and 15.1% respectively and those at 25, 50, 100, and 200 ppm were in order of Cortex(81.9$\sim$85.3%), Pip(33.4$\sim$83.0%), BHT(31.3$\sim$47.8%), Juice-2(15.4$\sim$36.8%) and Juice-1(13.4$\sim$36.1%).

석류씨, 석류 외피 분말 추출물 및 석류 과즙(주스 1과 주스 2) 추출물을 DPPH 라디컬 소거활동에 HepG-2 세포 그리고 항산화 활동에 추출물의 성장억제를 측정하였다. 높은 농도를 가진 모든 샘플은 HepG-2 세포에 대하여 성장 억제를 보였으며, 2,500 ppm의 처리구에서는 2회 감압 농축한 과즙 농축물(43%)>석류씨 분말 추출물(42%)>석류외피 분말 추출물(38%)>1회 감압 농축한 과즙 농축물(29%) 순으로 나타났다. BHT와 시료들 모두 농도 의존적으로 DPPH 라티칼 소거 활성이 증가하는 경향을 보였고, 12.5 ppm의 처리구에서 석류외피 분말 추출물(60.8%)>BHT(29.9%)>석류씨 분말 추출물(16.2%)>2회 감압 농축한 과즙 농축물(15.1%)>1회 감압 농축한 과즙 농축물(12.6%)의 순으로 나타났으며, 25, 50, 100 및 200 ppm처리구에서는 석류외피 분말 추출물(81.9$\sim$85.3%)>석류씨 분말 추출물(33.4$\sim$83.0%)>BHT(31.3$\sim$47.8%)>2회 감압 농축한 과즙 농축물(15.4$\sim$36.8%)>1회 갑압 농축한 과즙 농축물(13.4$\sim$36.1 %)의 순으로 나타났다.

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