• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.3
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• pp.175-179
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• 2007

In this study, we investigated the anti-obesity activity of extracts collected from wild plants in Jeju island. The inhibitory effect of plant extracts on the differentiation of preadipocyte 3T3-L1 was examined by oil red-O staining. We found that extracts collected from 6 plants among 31 plants, namely, Aralia elata(Miq.) Seem, Polygonum multiflorum Thunberg, Artemisia asiatica, platycodon grandiflorum(Jacq.) A. Dc., Polygonum cuspidatum S. et Z., Magnolia obovata Thunb, significantly inhibited preadipocyte differentiation. Additionally, 4 plant extracts were also found to have antioxidant activities in DPPH radical scavenging assay. Taken together, these results show that 6 plant extracts suppress the differentiation of preadipocytes, suggesting the potential use of 6 plant extracts as anti-obesity agents.

• Journal of Society of Preventive Korean Medicine
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• v.4 no.2
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• pp.57-71
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• 2000

BACGROUND: To examine effect of omahwan(烏麻丸)-赤何首烏(Polygonum multiflorum THUNB.), 白何首烏(Cynanchum wilfordii H.), 黑芝流(Sesamum indicum L.)-on the aged human immune system a series of humoral immunological parameters was compared in aged female (age 60-70 years) before and after administration of omahwan(烏麻丸). METHODS: Peripheral blood was obtained from fifteen healthy young (age 20-29, mean=66.3) female volunteers. B cell, T cell(T cells, T helper, and T suppressor/ cytotoxic) subsets was examined with specific monoclonal antibodies and isotype controls, using dural color flow cytometer IL-2 was examined with ELISA kit RESULT: By comparing the immune characteristics of the younger and elder groups the total ratio of T-cell, CD8 T-cell and quantify of IL-2 was significantly lower while CD4/CDB ratio was considerably greater in the elder group. ( p(0.05, student t-test) After giving Omahwan for 30 days to the elder group, by comparing the ratio before and after Prescription the total T cell and CD8 T cell ratio was considerably greater after prescription (p(0.05, Paired t-test) The quantity of IL-2 tended to increase after prescription but has no statistical meaning. CONCLUSION: The results indicate that administration of Omahwan(烏麻丸) differentially affects various aspects of the immune system in aged human.

• Journal of Society of Preventive Korean Medicine
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• v.9 no.1
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• pp.49-63
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• 2005

Objectives : Ojayeonjonghwan(五子衍宗丸) is composed of Polygonum multiflorum THUNB, and some medical herbs that are known as formula of senescence delay effects. The purpose of this study is to investigate th effect of Ojayeonjonghwan on antioxidant enzyme activities, such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione preoxidase (GSH-px) in rat erythrocytes and blood plasma. Methods : Sprague-Dawley rats were divided into 3 groups, Normal group (supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Ojayeonjonghwan administered group (D-galactose 50mg/kg and Ojayeonjonghwan extracts 245.0mg/200g 1time/day for 6 weeks, OJY Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : TBARS in plasma concentration of OJY group was significantly lower than that of control group. Red blood cell(RBC) SOD activity of OJY group was significantly higher than that of control group(F=16.057, p=0.0001, ANOVA test), RBC GSH-px activity of OJY group was increased(F=4.271, p=0.034, ANOVA test). RBC catalase activities of all experimental group were not significantly different. Total lipid and triglyceride concentration in plasma of all experimental groups were not significantly different. Total cholesterol concentrations in plasma of OJY group were significantly lower than those of control group(F=4.387, p=0.032, ANOVA test). Conclusions : According to the above results, it is considered that Ojayeonjonghwan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• Journal of Society of Preventive Korean Medicine
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• v.8 no.1
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• pp.115-133
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• 2004

Hasuohwan(何首烏丸) composed of Polygonum multiflorum Thunb and some medical herbs are known as formula of senescence delay effect. The aim of this study is to investigate the effect of Hasuohwan(何首烏丸) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS) in rat plasma and liver, Superoxide dismutase(SOD), Glutathione peroxidase(GSH-px), Catalase(CAT) in rat erythrocyte and liver. Rats were sacrificed and TBARS was measured in rat plasma and liver. SOD, GSH-px and CAT were measured in rat erythrocytes and liver. TBARS in plasma concentrations of HSO group was significantly lower than those of control group. RBC and liver GSH-px activities of HSO group were significantly higher than those of control group. According to above results, it is considered that Hasuohwan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat. Therefore, Hsuohwan is considered in effective of senescence delay.

• Journal of Society of Preventive Korean Medicine
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• v.7 no.1
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• pp.29-45
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• 2003

Objectives : Suoyounsoodan(首烏延壽丹) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect The purpose of this study is to investigate the effect of Suoyounsoodan(首烏延壽丹) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat plasma and liver. Methods: Sprague-Dawley rats divided into 4 groups, Young group(8 weeks old, N-8), Aging group(18 weeks old, N-18), pathologically induced aging group(injected D-galactose 50mg/kg, 1time/day for 6 weeks, CON) and Suoyounsoodan(首烏延壽丹) administered group(D-galactose 50mg/kg and Suoyounsoodan extracts 840.0mg/kg 1time/day for 6 weeks, SOY). Rats were sacrificed and TBARS, SOD, CAT, and GSH-px were mesured in rat plasma and liver. Results: Plasma and liver TBARS concentrations of SOY group was sinificantly lower than that of control. Red blood cell(RBC) SOD activities of SOY group was increased(F=3.405, p=0.034, ANOVA test), and RBC catalase activities of all experimental groups were not significantly different. RBC GSH-px activities of SOY group was increased(F=9.261, p=0.0001, ANOVA test). Liver SOD activities of SOY group was higher than that of control(F=3.806, p=0.023, ANOVA test). Liver catalase activities of all experimental groups were not significantly different, and liver GSH-px activity of SOY group was significantly higher than that of control(F=3.572, p=0.029, ANOVA test). Conclusions: According to the above results, It is considered Suoyounsoodan is effective in inhibiting lipid peroxidation and increasing anti oxidative enzyme activities in D-galactose induced aging rat.

• Journal of Society of Preventive Korean Medicine
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• v.6 no.2
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• pp.112-127
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• 2002

Objectives: Younnyeniksoobulrodan(延年益壽不老丹) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Younnyeniksoobulrodan(延年益壽不老丹) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase (GSH-px) in rat erythrocytes and liver. Methods: Sprague-Dawley rats divided into 4 gorups, Young group(8 weeks old, N-8), Aging group(18 weeks old, N-18), pathologically induced aging gorup(injected D-galatose 50mg／kg, 1time／day for 6 weeks, CON) and Younnyeniksoobulrodan(延年益壽不老丹) administered group(D-galactose 50mg／kg and Younnyeniksoobulrodan extracts 840.0mg／kg 1time／day for 6 weeks, YIB). Rats were sacrificed and TBARS, SOD, CAT, and GSH-px were measured in rat erythrocytes and liver. Results: Plasma and liver TBARS concentrations of YIB group were significantly lower than those of control. Red blood cell(RBC) SOD activities of YIB group was increased(F=3.445, p=0.033, ANOVA test), and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of YIB group was increased(F=9.365,p=0.0001, ANOVA test). Liver SOD activities of YIB group was higher than those of control(F=4.967, p=0.008, ANOVA test). Liver catalase activities of all experimental group were not significantly different, and liver GSH-px activity of YIB group was significantly higher than that of control(F=3.846, p=0.022,ANOVA test). Conclusions: According to the above results, it is considered that Younnyeniksoobulrodan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• Journal of Society of Preventive Korean Medicine
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• v.8 no.1
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• pp.75-87
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• 2004

Objectives: Rubi Fructus(fruit of Rubus coreanus Miq.) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Rubi Fructus(fruit of Rubus coreanus Miq.) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat erythrocytes and blood plasma. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Rubi Fructus (fruit of Rubus coreanus Miq.) administered group(D-galactose 50mg/kg and Rubi Fructus(fruit of Rubus coreanus Miq.) extracts 85.0mg/200g 1time/day for 6 weeks, BBJ Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : Plasma TBARS concentrations of all experimental group were not significantly different. Red blood cell(RBC) SOD activities of BBJ group was increased, and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of BBJ group was increased. Plasma total lipid concentration of BBJ group were significantly lower than those of control. Plasma triglyceride, total cholesterol and HDL-cholesterol concentrations of all experimental group were not signi ficantly different. Conclusions: According to the above results, it is considered that Rubi Fructus(fruit of Rubus coreanus Miq.) is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• Journal of Society of Preventive Korean Medicine
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• v.5 no.1
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• pp.76-89
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• 2001

OMH which is known for its properties of recruiting vitality, is composed of Polygonum multiflorum Thunb. and Sesamum indicum DC.. This formula is known to possess the properties of recruiting vitality, blackening white hair and expanding life span. 16-week-old SD-Rats were treated with OMH for 16 days. After 24 hours, the rats were treated with MTX(Mthotrexate is oral administrated for 4 days(1mg/kg/day), in order to lower immunity. Then, These rats were classified in to groups, the N-16 group(not specially tested), the Control group(MTX), the OMH-L group(2.5% OMH+MTX)&the OMH-H group(10% OMH+MTX) and 6 rats were assigned to each group. After 18 hours from MTX treatment the organ index of the rats from each group Thymus and Spleen were measured. The percentage of CD+4, CD8+ T cell were measured and compared by flow cytometer. 1) Rats from the OHM-L&OMH-H group showed higher organ indexes of the Thymus and Spleen compared to the rats from the Control Group. This proves that OMH possesses the properties to mitigate degenerations of immunity($F_{thymus}=20.162,\;F_{spleen}=5.882$, ANDVA, p<0.05). 2) The rats from the two OMH groups showed higher rates of CD4+ T cell counts compared to the control group(F=26.906, ANOVA, p<0.05). CD8+ T cell showed lower rates compared to the Control group, but showed no differences within the two OMH groups(F=1.254, ANOYA, p>0.05). CD4+/CD8+ showed higher rates in the two OMH groups compared to the control group which can be thought as a proof that OMH prevents depression of immune response(F=10.554, ANOVA, p<0.05). In this test 16 week-old rats were used, which can be considered as the middle and prime age of the human being. These rats were treated with OMH which ended up showing properties of mitigating degeneration of immune responses and maintaining T-cell rates within the blood. It was possible to study that OMH possesses the properties to increase immune responses.