• 생약학회지
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• 제23권1호
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• pp.9-13
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• 1992

From the leaves of Polygala japonica, kaempferol (1), astragalin (2), kaempferol $3-O-(6'-O-acetyl)-{\beta}-D-glucopyranoside$ (3) and kaempferol $3,7-di-O-{\beta}-D-glucopyranoside$ (4), have been isolated and characterized by chemical and spectral means.

• 생약학회지
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• 제27권4호
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• pp.316-322
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• 1996

Three medicinal species of the genus Polygala were examined by comparative morphology, anatomy and TLC analysis. They were classified into three species, and distinctly divided into two groups in this study. Group I is composed of P. japonica, P. sibirica and Group II P. tenuifolia. Considered on the relationships between two groups by the differences of leaf shape, adnated part of petal, flower colour, fruit shape etc., Group II may have been independently evolved from the common ancestor by having one raw of palisadelike chlorenchyma under the stem epidermis through the different pathway. It is also suggested that the taxa of Group II appear to be more advanced than those of Group I.

• 약학회지
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• 제1권1호
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• pp.1-2
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• 1948

The finely powdered entire harbs of Polygala japonica Houttuyn, a drug known as Yong Shin-Cho in Korea, were boiled with methanol and from the filtered extract the methanol was distilled off under diminished pressure and the aqueous solution of the residue was evaporated to dryness after being mixed with ignited magnesia. The dried mass was boiled with absolute alcohol, the filtered clear liquid was evaporated to a small volume and the precipitate Saponin produced by mixing with ether was filtered off. When the filtrate was again evaporated to the thickness of a syrup and allowed to cool for a few days in an ice box, crystalls were separated out in about 5% yield, which formed colorless columns, M. P. 142.deg., from methanol and had the formula $C_{6}$ $H_{12}$ $O_{5}$. On heating it with acetic acid anhybride and sodium acetate, its tetraacetyl derivative $C_{6}$ $H_{8}$ $O_{5}$(C $H_{3}$CO)$_{4}$ was obtained and which formed colorless needles, M. P. 62-5.deg., from ethanol. Their melting points, results of elementar analysis and other characteristics agreed with that of Polygalitol and its derivative. Finally they were proved to be identical with Polygalitol and its derivative, respectively, through determination of mixed melting points with the samples. Polygalitol was isolated from several plants of genus polugala e. g. P. amara, P. vulgaris, P. teunifolia, P. senega etc. The authors added to them another instance of identifying Polygalitol from the plant of genus polygala.olygala.

• 동의신경정신과학회지
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• 제22권4호
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• pp.185-199
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• 2011

In the present study, we assessed the effect of the PPA (Polygala japonica Houtt., Polygala tenuifolia WILLD. and Acorus gramineus SOLAND. mixed preparation), a traditional herbal prescription, on the learning and memory impairments induced by scopolamine. The cognition-enhancing effect of PPA was investigated using a passive avoidance test, Y-maze test and the Morris water maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine (1 mg/kg, i.p.). A single PPA (400 and 800 (mg/kg)) administration significantly reversed the scopolamine-induced cognitive impairments in the passive avoidance test (P<0.05). On the Y-maze test, PPA (400 and 800 (mg/kg)) also significantly reversed scopolamine-induced cognitive impairments in mice (P<0.05). PPA also improved escape latencies in training trials and increased swimming times and distances within the target zone of the Morris water maze (P<0.05). These results suggest that PPA attenuates amnesic state induced by scopolamine and that these Effect are mediated by enhancing the cholinergic dysfunction.

• 동의신경정신과학회지
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• 제22권2호
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• pp.85-105
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• 2011

Objectives : The purpose of this study was to characterize the neuroprotective effects and anxiolytic-like effects of Gongjin-dan and Polygala japonica, Polygala tenuifolia, Acorus gramineus mixed pills(G.J.D-P.P.A.). Methods : The neuroprotective effects of G.J.D-P.P.A. determined by the passive avoidance and Y-maze tasks and Morris water maze task, and the anxiolytic-like effects of the G.J.D-P.P.A. using an elevated plus-maze(EPM) in mice. Results : Drug-induced amnesia was induced by treating animals with scopolamine(1 mg/kg, i.p.). A single G.J.D-P.P.A.(400 and 800 mg/kg) administration significantly enhanced cognitive function and attenuated scopolamine-induced cognitive impairments as determined by the passive avoidance and Y-maze tasks(P < 0.05) and also reduced escape-latency on the Morris water maze task(P < 0.05). The administration of GJD-PPA(400 and 800 mg/kg) significantly increased the percentage of time spent in open arms and entries into the open arms of the EPM compared with saline-treated control group(P < 0.05). Moreover, there were no changes in the locomotor activity and myorelaxant effects in any group compared with saline-treated control group. Conclusions : These results suggest that GJD-PPA dramatically possesses the anti-amnestic and cognitive-enhancing activities related to the memory processes, and promotes the anxiolytic-like activity in mice.

• 동의신경정신과학회지
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• 제22권2호
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• pp.129-146
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• 2011

Objectives : The purpose of this study was to characterize the putative antidepressant and antianxiolytic effects of the 70% ethanol extract of Polygala japonica(EEPJ) using animal's behavioral experiment in mice. Methods : The effect of EEPJ on the anxioty and depressive disorder was investigated via mice's behavioral experiment like Elevated plus-maze, Horizontal wire test, Open field test, Forced swimming test, Tail suspension test, and it was happen via any mechanism by WAY 100635, a 5-HT1A receptor antagonist and by Flumazenil, a GABAA antagonist Results : 1. In the EPM, single treatments of the EEPJ(200 and 400mg/kg) had usefully antianxiolytic effects versus vehicle, which was medicated via the serotonergic nervous system. 2. In the HWT, single treatments of the EEPJ were no changes in the myorelaxant effects versus vehicle. 3. In the OFT, single treatments of the EEPJ were no changes in the locomotor activity versus vehicle. 4. In the FST, single treatments of the EEPJ(50mg/kg) significantly reduced the immobility time versus vehicle. 5. In the TST, single treatments of the EEPJ(50mg/kg) significantly reduced the immobility time versus vehicle. Conclusions : These results indicate that EEPJ is an effective antidepressant and antianxiolytic activity in mice, and it might be usefully applied for prevention and treatment of depressive disorder through evolutive study like development of various experimental models.

• Journal of Applied Biological Chemistry
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• 제61권1호
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• pp.51-57
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• 2018

영신초의 용매별 phenolic compounds는 열수 추출물과 추출 최적 농도인 50% 에탄올 추출물을 이용하여 실험을 진행하였다. 영신초 열수 추출물에 대한 항산화능을 DPPH, ABTS, PF, TBARs 실험으로 측정한 결과, $50-200{\mu}g/mL$의 농도 구간에서 각각 71.32-80.29%, 78.61-99.80%, 1.80-2.05 PF, 64.88-87.30%의 저해율을 나타냈고, 50% 에탄올 추출물에서는 각각 82.82-85.66%, 74.88-99.90%, 1.80-2.14 PF, 79.23-90.27%의 저해율을 나타냈다. 따라서, 영신초 50% 에탄올 추출물이 열수 추출물보다 항산화능이 더 우수한 것을 알 수 있었다. 영신초 열수 및 50% 에탄올 추출물의 미용식품 활성을 알아보기 위하여 tyrosinase 저해활성, elastase 및 collagenase 저해활성, hyaluronidase 저해활성을 측정하였다. Tyrosinase 저해활성은 $200{\mu}g/mL$의 농도에서 열수와 50% 에탄올 추출물은 각각 12.46, 22.10%를 나타냈다. Elastase 저해활성은 동일한 농도에서 각각 28.28, 58.36%, collagenase 저해활성은 각각 59.73, 78.90%를 나타냈으며, hyaluronidase 저해활성은 각각 35.65, 86.80%를 나타냈다. 또한 열수 추출물과 50% 에탄올 추출물 간의 생리활성의 차이는 phenolic profile의 차이에 의한 것이라 판단되었다. 이러한 결과에 따라, 영신초 열수 및 50% 에탄올 추출물은 항산화능이 높아 천연 항산화제로의 이용이 가능할 것으로 보이며, 특히 주름 개선 및 염증억제 효과가 뛰어난 것으로 나타나 기능성 식품 및 화장품 제조에 이용 가능한 천연 미용식품 소재로의 활용이 기대된다.

• 생약학회지
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• 제39권4호
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• pp.290-294
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• 2008

The objective of this study is screening the anti-oxidative effects of several plant MeOH extracts against oxidative stress in Neuroblastoma cell. Oxidative stress has been implicated in the pathogenesis of many neurotoxicity, neurodegenerative disorders and cell death. This oxidative stress is generated by ROS (Reactive Oxygen Species) such as nitric oxide, nitrogen dioxide, peroxyl, superoxide ($O_2^-$), hydroxyl, alkoxyl. So, in the present study, we induced oxidative stress by treatment of sodium nitroprusside (2.5 mM) in human neuroblastoma SH-SY5Y cell which was treated samples before 24hr, and cell viability was measured by MTT reduction assay. Of those tested, the extracts of Paeonia japonica (roots), Eucommia ulmoides (炒)(barks), Paeonia japonica (曝乾)(roots), Phyllostachys bambusoides (stems), Polygala tenuifolia (去心, 炒)(roots), Paeonia japonica (roots), Polygala tenuifolia (roots), Machilus thunbergii (barks), Mallotus japonicus (leaves), Poria cocos (whole), Sophora flavescens (roots), Angelica tenuissima (roots), Angelica gigas (當歸尾)(roots) showed anti-oxidative effects[$EC_{50}$<15.20 ${\mu}g$/ml(Carnosine:Positive control)]in dose dependent manner.

• Natural Product Sciences
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• 제7권2호
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• pp.38-44
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• 2001

NAD(P)H:quinone reductase (QR), known as DT-diaphorase, is a kind of detoxifying phase II metabolic enzyme catalyzing hydroquinone formation by two electron reduction pathway from quinone type compounds, and thus facilitating excretion of quinoids from human body. With the usefulness of QR induction activity assay system for the modulation of toxicants, in the course of searching for cancer chemopreventive agents from natural products, the methanolic extracts of approximately two hundreds of oriental medicines were primarily evaluated using the induction potential of quinone reductase (QR) activity in cultured murine Hepa1c1c7 cells. As a result, several extracts including Hordeum vulgare, Momordica cochinchinensis, Strychnos ignatii, Houttuynia cordata, and Polygala japonica were found to significantly induce QR activity. In addition, the methylene chloride fraction of H. vulgare, one major dietary food source, showed potent induction of QR activity $(CD=6.4{\mu}g/ml)$. Further study for isolation of active principles from these lead extracts is warranted for the discovery of novel cancer chemopreventive agents.

• The Korean Journal of Ecology
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• 제12권2호
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• pp.83-108
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• 1989

To discern general tendency in relatively pure even-aged pine stands, to group the stands and to perceive neighbourhood effects a total of 39 sites of pine stand was surveyed from nearby Seoul (12 sites), Chunsung, Kangwon (13 sites) and Sosan, Chungnam (14 sites), for herb and shrub species 32, 19; 37, 19 and 41, 14 in the respective areas from September 1987 to July 1988. In terms of detrended correspondence analysis (DECORANA), the stands were subjected to ordinate with 16 physical variables and the vegetational variables. The resource ratio (N:P, N:K, P:K) as physical variables also was tried out in the DECORANA as well as independent variable (N.P.K). The outcome did not show any meaningful difference. It is suggested that there seems to be no apparent interaction among the elements in the study. Three vertical vegetation componeent, that is, tree layer, shrub layer, herb layer were subjccted to DECORANA independently, pairwisely and as a whole (a total 7 combinations). Of those analysis herb layer trial alone seems to indicate relatively clearer differences among the physical variables. In the stands nearby Seoul first axis indicated soil field capacity and exchangeable cations (K, Ca and Na) and second axis did not show any tendency. For the Chunsung stands first axis also revealed soil field capcity and amount of arganic matter and second axis showed amount of exchangeable cation (K, Ca and Na), In the Seosan 1st axis indicated pH and exchangeable cations (K, Ca and Na). For the 39 sites 4 clusters in terms of herb layer might be defined: Peucedanum terebinthaceum-Cymbopogon tortilis-Polygala japonica-Festuca ovina (1); Atractylodes japonica-Patrina scabiosaefolia (2); Potentilla fragarioides-Atractylodes (3); and Cymbopogon tortilis (4). In the neighbourhood effects study in terms of the basal area distribution, Thiessen polygon area and Gini coefficient for the Pinus thunbergii stands of Seosan the Thiessen polygon area approach seems to indicate earlier (30 years old) neighbourhood effect than the others (45 years).