• Molecular & Cellular Toxicology
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• 제2권1호
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• pp.11-14
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• 2006

In this study we attempted to develop a novel genomic method to selectively isolate target functional microbial genomes from environmental samples. For this purpose, stable isotope probing (SIP) was applied in selectively isolating organic pollutant-assimilating populations. When soil microbes were fed with $^{13}C-labeled $ biphenyl, biphenyl-utilizing cells were incorporated with the heavy carbon isotope. The heavy DNA portion was successfully separated by CsCl equilibrium density gradient. And the diversity in the heavy DNA was sufficiently reduced, being suitable for the current DNA microarray techniques to detect biphenyl-utilizing populations in the soil. In addition, we proposed a new way to get more genetic information by combining this SIP method with selective metagenomic approach. The increased selective power of these new DNA isolation methods will be expected to provide a good quality of new genetic information, which, in turn, will result in development of a variety of biomarkers that may be used in assessing ecotoxicology issues including the impacts of organic hazards, and antibiotic-resistant pathogens on human and ecological systems.

• 한국대기환경학회지
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• 제21권4호
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• pp.439-458
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• 2005

An automated analysis system for water soluble constituents in $PM_{2.5}$ has been developed. The system consists of a high capacity multi tube diffusion scrubber (MTDS), a low temperature particle impactor (LTPI), and two ion (anion and cation) chromatography (IC) systems. Atmospheric particles have been collected by passing sample air through a thermostated MTDS followed by a LTPI. This system allows simultaneous measurements of soluble ions in $PM_{2.5}$ at 30 minutes interval. At the air sampling flow rate of 1.0L/min, the detection limits of the overall system are in the order of tens of $ng/m^3$. This system has been successfully used for the measurement of particulate components of Seoul air from April 2003 to January 2004. $SO_4^{2-},\;NO_3^-,\;NH_4^+,\;NO_2^-,\;Cl^-,\;Na^+,\;K^+,\;Ca^{2+},\;and\;Mg^{2+}$ are the major ionic species for $PM_{2.5}$ at Seoul. Among them, $SO_4^{2-},\;NO_3^-\;and\;NH_4^+$ are the most abundant ions, contributed up to $86\%$ of the total and the concentrations were higher than those in any other urban sites in the world except for Chinese cities. There are high pollutant episodes which contribute about $15\~20\%$ of annual average values of the major ions. During the episode, the all parcels were transported from the asian continent and $PM_{2.5}$ were significantly neutralized. This suggests that aged and long range transported pollutants caused the high pollutant episodes. They showed a distinct daily and seasonal variations:they showed a peak in the early morning caused by the night-time accumulation of particulate matters. Atmospheric reactions including gas-to-particle reactions and inter-particle reactions and meteorological parameters including relative humidity and ambient temperature were described with related to the $PM_{2.5}$ 5 concentrations. All of the ionic species showed higher concentrations during the spring than those for summer and winter.

• 전기전자학회논문지
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• 제13권4호
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• pp.37-42
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• 2009

본 연구에서는 졸겔법으로 ZnO, 수열합성법으로 $SnO_2$ 나노분말을 제조하고 이들 나노분말에 Pd, Ru 등의 촉매를 첨가하였다. MEMS 기술로 제작된 히터 및 전극 구조 위에 나노 감지 분말을 도포하여 CO and $NO_2$ 가스 센서를 제작하였다. 0.1 wt% Pd 도핑된 $SnO_2$ 가스센서와 Ru 도핑된 ZnO 가스 센서는 각각 CO 30 ppm, $NO_2$ 1 ppm의 낮은 농도에서도 높은 감지 특성을 보였다.

• 센서학회지
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• 제3권2호
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• pp.33-39
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• 1994

냄새물질 특히, 환경오염물질의 측정을 위한 수정진동자를 이용한 센서 시스템을 수정진동자 표면에 여러가지 인지질과 활성탄 그리고 납계열 무기안료를 피막시켜 만들었다. 포스파티딜 콜린을 누적한 9MHz 수정진동자의 각종 유기가스에 대한 응답 특성을 살펴보았다. 인지질막의 특성에 기초한 서로 다른 냄새물질사이의 친화력에 대하여 설명할 수 있었다. 냄새물질은 누적한 지질막에 의존하는 공진주파수 변화 패턴과 응답면적에서 계산되어지는 상대적인 응답 세기를 서로 비교하여 확인할 수 있었다. 납계열 무기안료를 누적한 수정진동자를 이용하여 황화물의 검출이 가능함을 알 수 있었다. 이 결과, 여러가지 지질을 누적한 수정진동자를 이용하여 그 응답패턴을 비교한다면 냄새물질의 분석이 가능함을 알았다.

• 한국응용과학기술학회지
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• 제28권1호
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• pp.64-74
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• 2011

The aim of study was to investigate polluted amounts 16 PAHs in railroad(waste railway ties) area, factory area and land-fill area. All of the samples were analyzed by GC-MSD(SIM mode), and the recovery range, detection limit and standard deviation obtained by this experiment were 73.88 ~ 94.75%, 0.009 ~ 2.252 ${\mu}g$/kg and 1.861 ~ 12.373, respectively. The concentrations of total PAHs(t-PAHs) and total carcinogenic PAHs(t-PAHcarc) in soils of three area were in the range of 12.54 ~ 3274.95 ${\mu}g$/kg on a wet weight basis with a mean value of 499.8 ${\mu}g$/kg and 0 ~122.77 ${\mu}g$/kg with a mean value of 20.16 ${\mu}g$/kg, respectively. The correlation between t-PAHs and t-PAHcarc appeared very high in railroad(waste railway ties) area ($R^2$ = 0.8301), factory area ($R^2$ = 0.9217) except land-fill area($R^2$ = 0.3782), indicated that t-PAHcarc concentration increases in proportion with t-PAHs.

• 공업화학
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• 제16권3호
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• pp.323-327
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• 2005

Pentachlorophenol (PCP)은 독성이 강한 발암성 물질로 미국환경청(Environmental Protection Agency, USA)이 정한 대표적인 환경호르몬이다. 본 연구에서는 종이 중에 함유된 극미량의 PCP 측정을 목적으로 하였다. 종이의 전처리는 고상추출법(SPE)을 이용하였고 분석을 위해 PCP를 유도체화시켜 gas chromatography/mass spectroscopy-selected ion monitoring (GC/MS-SIM)으로 분석하였다. GC/MS-SIM의 분석 결과 실릴화 PCP의 분석감도가 증가되었다. 본 실험에서는 실험군으로 사무용지와 벽지를 사용하였다.

• 한국대기환경학회지
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• 제21권6호
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• pp.573-583
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• 2005

Fugitive dust, which is emitted in the ambient air without first passing through a stack or duct designed to control flow, is frequently generated by means of wind erosion from storage yards at Pohang Steel Wokrs. The size distribution of fugitive dust is mostly in the range of coarse particulate which is deposited as soon as emitted and less harm to human health; however $20\%$ of fugitive dust contains PM 10 known as one of most harmful airborne pollutant. Consequently, effective control and reduction of fugitive dust is strongly requested by the local society, but it is not easy so far because the generation and dispersion of fugitive dust highly depends on meteorological conditions, and it being occurred for irregularity. This research presented a fugitive dust control system for each meteorological condition by providing statistical prediction data obtained from a statistical analysis on the probability of generating the threshold velocity at which the fugitive dust begins to occur, and the frequency occurring by season and by time of the wind direction that can generate atmospheric pollution when the dispersed dust spreads to adjacent residential areas. The research also built a fugitive dust detection system which monitors the weather conditions surrounding storage yards and the changes in air quality on a real-time basis and issues a warning message by identifying a situation where the fugitive dust disperses outside the site boundary line so that appropriate measures can be taken on a timely basis. Furthermore, in respect to the spraying of water to prevent the generation of fugitive dust from the storage piles at the storage yard, an advanced statistical meteorological analysis on the weather conditions in Pohang area and a case study of fugitive dust dispersion toward outside of working field during $2002\∼2003$ were carried out in order to decide an optimal water-spraying time and the number of spraying that can prevent the origin of fugitive dust emission. The results of this research are expected to create extremely significant effects in improving surrounding environment through actual reduction of the fugitive dust produced from the storage yard of Pohang Steel Works by providing a high-tech warning system capable of constantly monitoring the leakage of fugitive dust and water-spray guidance that can maximize the water-spraying effects.

• 수산해양기술연구
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• 제47권1호
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• pp.27-36
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• 2011

Squid by-product like viscera has just been recognized as one of the serious pollutants on the surrounding marine environment in Ulleung island of Korea. The effect as a marine pollutant, however, has not been examined and evaluated properly. It was carried out the comprehensive examination of the marine pollution by disposing squid by-products. The sediments and water qualities were investigated in several positions, including the dumping position around Ulleung island. It is also estimated a spreading of by-product in terms of estimating the sinking speed and evaluating the attracting characteristics as a feed. As a result, There were no significant pollutions of the sediment and water quality. The spreading of by-product by the local current was 2-2.5 times of the depth, and it will become longer in spring tide. It was confirmed that the squid by-product has attracting ability for local fish by acoustical survey. Furthermore, it can be useful for the local fish species as feeds.

• 한국환경농학회지
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• 제17권1호
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• pp.59-64
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• 1998

The environmental problem of the rural area has been accelerated in soil as well as water. Soil contamination is usually caused by improper operation of landfills, abandoned mine fields, accidental spills, and illegal dumpings. Once soil contamination is initiated, pollutants migrate and may cause groundwater contamination which takes much effort for remediation. Early detection, therefore, is important to prevent further contamination. Electrical resistivity method was used to detect soil contamination, but it was not effective to the heterogeneous condition. Static cone penetrometer test (CPT) has been used widely to investigate geotechnical properties of the underground. In this study, electrical resistivity method and CPT are combined to improve the applicability of it. The pilot test was performed to examine the variation of electrical resistivity with different soil minerals and pore fluid characteristics. Soil samples used were poorly graded sand, silty sandy soil, and weathered granite soil. For all the cases, electrical resistivity decreased with increasing of moisture content. Soil mineral also affected the electrical resistivity significantly. Above all, leachate addition in the pore fluid was very sensitive and caused decreasing of electrical resistivity markedly. It implies that electrical resistivity method can be applied to investigate pollutant plume effectively. This is specially sure when the sensors contact the contaminated soils directly. The CPT method involves cone penetration to the ground, therefore, underground contamination around the cone could be investigated effectively even for heterogeneous condition as it penetrates if electrical resistivity sensors are attached on the cone.

• 인간식물환경학회지
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• 제23권5호
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• pp.537-544
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• 2020

Background and objective: Artemisia argyi has a long history as an effective treatment for various diseases. The detection of environmental pollutant benzo(a)pyrene, a known human carcinogen, in the leaves of Artemisia argyi is cause for concern. For medicinal plant extracts, both a reduction of benzo(a)pyrene as well as the maintained effectiveness of the compound are important. Therefore, in this study, we propose an optimized process for the addition and filtration of activated carbon to reduce benzo(a)pyrene and change the contents of the indicating substance(jaceosidine and eupatilin). Methods: Artemisia argyi EtOH extract containing 36 ppb of benzo(a)pyrene was added to 0.1, 0.5, 1.0, and 1.5% (w/w) of activated carbon for 120 min and filtered using an activated carbon filter 1, 2, 3, and 5 times respectively. The content of benzo(a)pyrene and indicating substances in Artemisia argyi extract were then measured with high performance liquid chromatography (fluorescence and UV detectors). Results: As the amounts of activated carbon powder and filtering cycles increased, the content of benzo(a)pyrene in the Artemisia argyi extract decreased. However, when activated carbon powder 1.5% was added to the extract, and when the activated carbon filter was filtered five times, the results were reduced by 15% and 30~40% respectively. The optimal extraction condition for reducing benzo(a)pyrene was adding 1.5% of activated carbon powder. This resulted in reducing benzo(a)pyrene by 83% and indicating substances by about 4%. Conclusions: Here we present a process for reducing benzo(a)pyrene in Artemisia argyi extract using activated carbon to reduce toxicity and minimize the loss of active ingredients. This approach has potential application within a manufacturing process of various medicinal plant extracts.