• Journal of Korean Society of Forest Science
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• v.23 no.1
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• pp.9-16
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• 1974

The anthers of late uninucleate microspore or early binucleate microspore stage of Paeonia suffruticosa Andr. (economic tree) were cultured on the modified Murashige and Skoog's medium suppliment with Keinetin, 2,4-D, and NAA for inducing haploid plants. The results are as follows; 1. Callus were induced from both anther locule and anther wall, where that from anther locule was identified as haploid. 2. Among 2,000 anthers cultured, fourteen haploid callus were developed. These haploid callus were clearly identified to be originated from the microspore in anther locule. 3. Diploid callus were induced only 0.5 percent from the callus of endothelium of anther wall, septium of two neighboring anther locule, parenchyma tissues of connectives and or anther locules. 4. In the anther locule of Paeonia suffruticosa Andr. cultured in medium, swollen microspores, polynucleate microspores, multicellurar pollen grains, or callus mass was frequently observed. And the haploids were seemed to be caused by the callus originated from the reduced microspore.

• Korean Journal of Plant Taxonomy
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• v.42 no.2
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• pp.141-149
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• 2012

To clarify some ambiguous diagnostic characters of Callicarpa japonica Thunb. and Callicarpa dichotoma (Lour.) K. Koch in Korea (Verbenaceae), the external morphology and micromorphology (leaf, anther, pollen, fruit, in particular endocarp surface and structure) of two taxa are studied and described in detail. It is confirmed that the following characteristics (e.g., the stem outline in a cross-section, the bud shape and length, the corolla tube length and the lobe length, the pattern of the anther dehiscence and length, and the endocarp morphology) are useful for distinguishing these two taxa. In particular, following three characteristics are most useful for an identification: (1) the corolla tube length of C. japonica (2.5-4.6 mm) is longer than that of C. dichotoma (0.7-1.0 mm); (2) the anther is dehiscing by an apical pore at the anthesis of C. japonica, while it is opening by a long fissure from the apex to the base in C. dichotoma; and (3) the edge of the endocarp (lateral view) in C. japonica is concave, while in C. dichotoma it is shown to be flat. On the other hand, the teeth state on the leaf margin and the position of inflorescence are not good diagnostic characteristics for identification. Additionally, the keys for all known Callicarpa taxa in Korea are provided.

• Korean Journal of Plant Taxonomy
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• v.40 no.4
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• pp.226-233
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• 2010

An intensive study of the embryology of Gymnospermium microrrhynchum was conducted to provide information regarding a discussion of the phylogenetic relationships of the genus, which is yet unstudied. Our results indicated that Gymnospermium is similar to other genera of Berberidaceae in terms of its embryological features. Nevertheless, newly reported and unique features are the well-developed endothelium and the undifferentiated seed coat type. Until the study of Gymnospermium, it may have been considered to be closer to Caulophyllum and Leontice in the tribe Leonticeae. These three genera share many morphological features as well as molecular similarities, by which they are kept in the same tribe, Leonticeae. However, very little detailed embryological data regarding these genera have been published thus far. Gymnospermium was characterized according to the basic type of anther wall formation as well as its glandular tapetum, successive cytokinesis in the microspore mother cell, two-celled mature pollen grains, anatropous and crassinucellate ovules with a nucellar cap, well-developed endothelium, its Polygonum type of embryo sac formation, its nuclear type of endosperm formation, and its undifferentiated seed coat type. In comparison with Nandina, there are many differences, such as the dehiscence of the anther, the cytokinesis in the microspore mother cells, the shape of the megaspore dyad, and the seed characteristics. Although we had no available detailed embryological information regarding Caulophyllum and Leontice, which are genera that are more closely related to Gymnospermium, we could deduce from the phylogenetic relationship that Gymnospermium, Caulophyllum, and Leontice are more closely related to each other than other genera of Berberidaceae on the basis of the seed characteristics.

• Journal of the Korean Geographical Society
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• v.44 no.6
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• pp.691-705
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• 2009

Phytolith analysis was made on a 660cm core from Lake Gyeongpo in the East Sea of Korean Peninsula to clarify the environmental change including climate and agricultural characteristics during the Holocene. From the results of phytolith analysis, six phytoliths assemblage zone(PAZ) were recognized from the base to the surface. PAZ I around 5,000 yr BP suggests the transition from the warm and dry to the cool and wet climatic conditions. The climate of PAZ II(ca. 4,000-2,000 BP) was kept on warm, but repeated between dry and wet conditions. PAZ III(2,000~1,000 yr BP) suggests the expansion of agricultural activities under the warm and humid climatic conditions due to the significant phytoliths production of Paniceae and Oryza sativa. While PAZ IV(1,000~500 yr BP) indicates very cool and dry conditions, PAZ V and IV suggest the warm-dry and cool-humid climatic conditions, respectively. Similar to the results of pollen analysis in the lake, the agricultural activities were recognized by PAZ III around 2,000 yr BP from the results of phytolith analysis, and the rice cultivations such as Oryza sativa have been expanded since 2,000 yr BP or later.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.173-176
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• 2016

This research was performed to breed new columnar-typed apple variety for pollinator in apple orchard. We cross-fertilized 'Hongro' that was an ovary parent and 'Maypole' a pollen parent in 2003. We gathered seeds in 2003 and sowed after breaking of dormancy in 2004. Seedlings was been growth and development till flowering. After fruit setting, We investigated characteristics of growth and fruit including mating compatibility from 2010 to 2012, then finally selected '03-2-95' named 'Tinkerbell' in 2012, registered as new variety on Korea Seed & Variety Service in 2015. The full bloom stage of 'Tinkerbell' was later 2 days then of 'Maypole'. Tree habit of 'Tinkerbell' was alike to of 'Maypole', for example weak tree vigor, columnar type. But there were many different characteristics between 'Tinkerbell' and 'Maypole'. Fruit shape of 'Tinkerbell' was ovoid whereas of 'Maypole' was ellipsoid. Fruit of 'Tinkerbell' was bigger, smoother skin, no waxy bloom than of 'Maypole'. Fruit of 'Tinkerbell' was more delicious than of 'Maypole' because of having higher soluble sugar content and lower titratable acidity. 'Tinkerbell' was excellent pollinator in apple orchard. Because of having high fruit setting rate with major apple varieties and a narrow tree width that was advantageous as pollinator in apple high density system.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.119-125
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• 2015

Interspecific crosses between rapeseed (Brassica napus L.) and chinese cabbage (B. campestris var. pekinensis Makino) and turnip (B. rapa L.) were made in order to examine the cross possibility and morphological phenotype of $F_1$ hybrid. The growth of pollen tube of cross between rapeseed and chinese cabbage was more rapid than cross between rapeseed and turnip. Silique formation and seed setting in silique were relatively high in the cross between rapeseed and chinese cabbage. The percentage of silique set from interspecific cross between rapeseed and chinese cabbage was 90.6% and higher 23.3% than the percentage of silique from interspecific cross rapeseed and turnip. The average number of seed per silique obtained from the cross rapeseed and chinese cabbage, and rapeseed and turnip reached 15.5 and 11.6, respectively. The morphological phenotypes of $F_1$ hybrid plants obtained from seeds in the cross between rapeseed and chinese cabbage resembled rapeseed mainly, but leaf length and leaf width were increased. The size, shape and lobation of leaves of $F_1$ hybrid plants from interspecific cross between rapeseed and turnip were intermediated between their parent species, but color of leaves was dark-green.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.81-85
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• 1994

This experiment was carried out to investigate the fertilization and the size of mature female and male gametophytic parts of Pulsatilla koreana after artificial pollination. The size of pollen is $26.5{\mu}m$ at the time of anther dehiscence, that is, about $3{\sim}4$ days after pollination. Synergid nucleus, egg nucleus, and polar nucleus are 10.0, 15.0, and $32.5{\mu}m$ respectively at the time of completing egg apparatus formation, that is, about 2 days after pollination. Poller tubes germinate on stigma about 10 hours, passing lower part of style about 30 hours, penetrating into micropyle about 35 hours after pollination. Sperm nucleus penetrates into polar nucleus about 40 hours and egg cell about 48 hours after pollination. But, there seems to be different among the individuals. Multinuclei and multinucleoli are formed in egg cell, synergid, and polar nucleus about the time of fertilization. Proembryo is formed about 4 days, being changed to large globular form about $6{\sim}8$ days after pollination. Endosperm nuclei divide into free nuclei after fertilization and change to cotyledon in gymnosperm. There seems to be same phenomena in Pulsatilla koreana.

• The Korean Journal of Quaternary Research
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• v.20 no.1 s.26
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• pp.39-50
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• 2006

Jeonjangri site of Geochang area is located in the Geochang Basin, and lies on the river terrace of upstream part of Hwang River. Fluvial deposits are well distributed at the northern and southern walls of trench 2(district 2) in the Jeonjangri archeological site. This study aims to interpret the occurrences of fluvial sedimentary deposits on the basis of grain size analysis and palynological analysis in the representative sections of Jeongjangri site. The sedimentary profile shows that the upper units are typified by paleosols with soil wedge formed at about $25,000{\sim}30,000yr$ B.P, and the lower units are characterized by reddish brown muddy sands, organic muds and sand/gravel downwards in the profile. Particularly palynological study on the organic muds of southern wall section showed a result that lower unit is dominant with grass vegetation, and upper unit with Alnus-Quercus-Pinus vegetation. The former is interpreted to be formed at $60,000{\sim}50,000yr$ B.P (stadial), while the latter at $80,000{\sim}70,000yr$ B.P. In general broad-leaved/coniferous mixed forests are mostly dominant in Jeongjangri site and the climate was presumed to be cool temperate at that time.

• Analytical Science and Technology
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• v.16 no.1
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• pp.1-11
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• 2003

This research examined the presence of hazardous materials in chemical workplace field using an integrated chemical/biological monitoring. Chemical workplace field air for volatile organic compounds (VOCs) analysis was collected using a collection tube packed with Tena.x TA adsorbent 400 mg. Workplace field air samples were analyzed by gas chromatography/mass spectrometry (GC/MS). Simultaneously, Tradescantia BNL 4430 clone was exposed in situ to monitor hazardous materials in chemical workplace field. GC/MS analysis showed the presence of various VOCs such as trichloroethylene, toluene, ethylbenzene, (m,p,o)-xylenes, styrene, 1,3,5-trimethylbenzene, and 1,2,4-trimethylbenzene. The results showed that in situ monitoring of VOCs with the Tradescantia-micronucleus (Trad-MCN) assay gave positive results in chemical workplace field and negative response at outdoor air. In conclusion, inhalation of these field air by workers may affect chronic demage to their health by inducing micronuclei formation in Tradescantia pollen mother cells. The combination of chemical/biological monitoring is very effective to evaluate hazardous materials in workplace field and can be alternatively used for screening hazardous materials.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.135-139
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• 1998

Reciprocal interspecific hybrids between N. glauca(2n=24) and N. langsdorffii(2n=18) were obtained by intercrossing. One hundred percent of F$_1$ seeds was produced from intercrossing of N. glauca $\times$ N. langsdorffii, whereas the frequency of F$_1$ hybrid seed formation from N. langsdorffii $\times$ N. glauca was very low. However, all the hybrid seeds were germinated well and then grown to normal plantlets. All the plants of F$_1$ hybrids have chromosome number of interspecific hybrids (2n=21). From observation of morphological characteristic, the structure of petrol, leaf, flower, and the morphology of pollen have characteristics of F1 hybrid. Spontaneous tumors (genetic tumor) were formed from each F$_1$ hybrid; the genetic tumor arose at the reproductive phase when the maternal type of F$_1$ hybrid came from N. glauca, while the genetic tumor arose only after reproductive phase when the maternal type of F$_1$ hybrid came from N. langsdorffii. The genetic tumor actively proliferated on hormone-free medium and produced numerous teratoma shoots. In addition, normal leaf or stem explants of F$_1$ hybrid produced calli on hormone-free medium after 15 days of culture, the calli produced new numerous teratoma shoots after 30 days. The frequency of teratoma shoot formation from rnterspecific hybrid was higher in the N. glauca $\times$ N. langsdorffii than in the N. langsdorffii $\times$ N. glauca. Root development from the teratoma shoots was hardly obtained. Teratoma shoots without roots in vitro can form genetic tumor at the vegetative growth phase after tissue culture.