• Title/Summary/Keyword: Polar education

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Crystal Structure and Comparative Sequence Analysis of GmhA from Colwellia psychrerythraea Strain 34H Provides Insight into Functional Similarity with DiaA

  • Do, Hackwon;Yun, Ji-Sook;Lee, Chang Woo;Choi, Young Jun;Kim, Hye-Yeon;Kim, Youn-Jung;Park, Hyun;Chang, Jeong Ho;Lee, Jun Hyuck
    • Molecules and Cells
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    • v.38 no.12
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    • pp.1086-1095
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    • 2015
  • The psychrophilic organism Colwellia psychrerythraea strain 34H produces extracellular polysaccharide substances to tolerate cold environments. Sedoheptulose 7-phosphate isomerase (GmhA) is essential for producing $\small{D}$-glycero-$\small{D}$-mannoheptose 7-phosphate, a key mediator in the lipopolysaccharide biosynthetic pathway. We determined the crystal structure of GmhA from C. psychrerythraea strain 34H (CpsGmhA, UniProtKB code: Q47VU0) at a resolution of $2.8{\AA}$. The tetrameric structure is similar to that of homologous GmhA structures. Interestingly, one of the catalytic residues, glutamate, which has been reported to be critical for the activity of other homologous GmhA enzymes, is replaced by a glutamine residue in the CpsGmhA protein. We also found differences in the conformations of several other catalytic residues. Extensive structural and sequence analyses reveal that CpsGmhA shows high similarity to Escherichia coli DnaA initiatorassociating protein A (DiaA). Therefore, the CpsGmhA structure reported here may provide insight into the structural and functional correlations between GmhA and DiaA among specific microorganisms.

An Analysis of Pre-Service Teachers' Reflective Thinking for Tasks on Polar Coordinates

  • Oh, Hye Mi;Park, Jee Hyun;Kwon, Oh Nam
    • Research in Mathematical Education
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    • v.17 no.2
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    • pp.119-131
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    • 2013
  • It is the first time that there is a subject, advanced mathematics in the 2009 revised high school curriculum. Therefore it is posing a challenge to the teachers who are teaching it. At the advanced level, it is important for learners to reflect on their mental mathematical activities. This research analysed pre-service secondary teachers' reflective thinking in solving the tasks specific for the teaching and learning of polar coordinates. We report how and through what process mathematical tasks that can create disequilibrium for pre-service secondary teachers enable reflective thinking and expand preservice secondary teachers' thoughts and recognition of defining reflective thinking in looking back on one's problem solving and thinking processes.

EFFICIENT PLOTTING OF CLOSED POLAR CURVES WITH MATHEMATICA

  • Lee, Kwang-Bok;Kim, Young-Ik
    • The Pure and Applied Mathematics
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    • v.5 no.2
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    • pp.133-142
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    • 1998
  • A simple mathematical theory is developed on the periodicity of elementary polar functions. The periodicity plays an important role in efficient plotting of some closed polar curves, without the excessive use of plotting devices and materials. An efficient plotting algorithm utilizing the periodicity is proposed and its implementation by a Mathematica program is introduced for a family of closed polar functions.

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Biological Activity Analysis of Potato-derived Polar Compounds (감자 유래 극성화합물의 생리활성 분석)

  • Kim, Dae Yoon;Nam, Jung Hwan;Lee, Jae Kwon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.62-62
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    • 2019
  • Natural substances have various physiological activities. Substances isolated from natural substances are known to be safer and more potent than pharmaceuticals. Potatoes not only act as energy sources but also contain active ingredients such as vitamins and minerals. In particular, the potato contains a large amount of polar compounds, including the saponin in the polar compounds, and the physiological activity of the saponins, such as immunity enhancement, antioxidant and anti-inflammatory is known. In this study, the antioxidative activity of polar compounds from five potatoes was examined by chemical base anti-oxidation assay and cell based anti-oxidation assay. In the chemical base anti-oxidation assay, DPPH experiment showed activity in the order of Hongyoung, Haryung, Seohong, Sumi, and Jayoung. In the LPA experiment, IC50 was lower in the order of Jayoung, Seohong, Sumi, Hongyoung, and Haryung. In the cell based anti-oxidation assay, the smallest amount of ROS was generated when the compound was derived from Haryung and hongyoung, and strong SOD activity was observed in Sumi and Jayoung. The results of this study reveal the antioxidative effect of polar compounds extracted from various kind of potatoes, which will enable the acquisition of new bioactive candidates and the establishment of new profit generation models for farmers.

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Envelope of the Wallace-Simson Lines with Signed Angle ${\alpha}$

  • Bae, Sung Chul;Ahn, Young Joon
    • Journal of Integrative Natural Science
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    • v.5 no.1
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    • pp.38-41
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    • 2012
  • In this paper we show that for any triangle and any point on the circumcircle the envelope of the Wallace-Simson lines with signed angle ${\alpha}$ is a parabola. The proof is obtained naturally using polar coordinates. We also present the reparametrization of the envelope which is a linear normal curve.

Limitations of the Transition State Variation Model(5) Dual Reaction Channels for Solvolysis of Dansyl Chloride

  • Koo In Sun;Lee Ocg-Kyeong;Lee Ikchoon
    • Bulletin of the Korean Chemical Society
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    • v.13 no.4
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    • pp.395-398
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    • 1992
  • Rate of solvolysis of dansyl chloride in aqueous binary mixtures of acetone, methanol and ethanol are reported. Kinetic solvent isotope effects in methanol and product selectivities in alcohol-water mixtures are also reported. Kinetic data are interpreted with the Grunwald-Winstein and Kivinen equations. The value of $k_{CH3OH}/k_{CH3OD}=1.76$ suggests that a general have catalyzed and/or an $S_AN$ pathway is operative in methanol, a less polar solvent. Rate-rate profiles for solvolysis of dansyl chloride in the aqueous binary media indicate a change in reaction channel from $S_AN$ (in less polar media) to $S_N2$ (in more polar media) mechanism.

Measurement of 137Cs in Ice Core Samples from Antarctica

  • Lim, S.I.;Kim, D.H.;Huh, J.Y.;Lee, J.;Hahn, I.S.;Han, Y.C.;Hur, S.D.;Hwang, H.J.;Kang, W.G.;Kim, Y.D.;Lee, E.K.;Lee, M.H.
    • Journal of the Korean Physical Society
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    • v.73 no.9
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    • pp.1263-1268
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    • 2018
  • Three different ice core samples from Antarctica were analyzed to identify activity concentrations of radioactive isotopes. Tracking migration of radioactive isotopes to Antarctica can provide a key clue to understand global environmental changes caused by radiation exposures because the Antarctic ice cores can preserve unique characteristics of various environmental conditions. We are particularly interested in the $^{137}Cs$ nucleus, because it is closely related to radiation exposure from nuclear power plant accidents and nuclear bomb tests. With its half life of $30.17{\pm}0.03$ years, $^{137}Cs$ can also be used to assess the age of sedimentation occurring after around the year 1945. We selected three ice core samples, called Tarn8, Styx27, and H25, from different time periods; the Tarn8 sample is known to be from earlier than ~ 1000 AD, the Styx27 sample is approximately from the year 1945, and the H25 sample is from the year 2012. Radioactive isotope measurements of the ice core samples were performed using a 100% HPGe detector at Cheongpyeong Underground Radiation Laboratory (CURL). We measured the activity of $^{137}Cs$ in the H25 sample to be $0.98{\pm}0.82mBq/kg$. Although the activity has a large uncertainty mainly due to the limited sample quantity, the $^{137}Cs$ isotopes in the Antarctic ice core were measured for the first time in Korea.

Detection of Long Period Seismic Events by Using a Portable Gravity Meter, gPhone (이동식 중력계(gPhone)를 활용한 장주기 지진 이벤트 관측)

  • Lee, Won Sang;Seo, Ki-Weon;Eom, Jooyoung;Sheen, Dong-Hoon;Lee, Choon-Ki;Park, Yongcheol;Yun, Sukyoung;Yoo, Hyun Jae
    • Geophysics and Geophysical Exploration
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    • v.18 no.1
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    • pp.31-34
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    • 2015
  • A gravity meter has been used for exploring subsurface mineral resources and monitoring long-period events such as Earth tides. Recently, researchers found several other intriguing features that we could even detect large teleseismic earthquakes and monitor seismic ambient noise using gravimeters. The zero-length spring suspension technology gives the gPhone (Micro-g LaCoste) excellent low frequency sensitivity, which may have implications for investigating much longer-period natural events (e.g., Earth's hum, tsunami waves, etc.). In this study, we present preliminary results through temporary operation of the gPhone at Geumsan in South Korea for 9 months (Nov. 2008-Jul. 2009). The gPhone successfully recorded large teleseismic events and showed a clear seasonal variation of the Double frequency microseisms during its operation period.

Different Developmental Competence of Porcine Oocytes Selected by Brilliant Cresyl Blue Staining and Polar Body Extrusion (Brilliant Cresyl Blue 염색방법과 극체 방출 여부에 따른 돼지 체외수정용 난포란 선별 방법이 배발달에 미치는 영향)

  • Kim, Yeon-Soo;Kim, Cheol-Wook;Kim, In-Cheol;Kwack, Dae-O;Chung, Ki-Hwa
    • Reproductive and Developmental Biology
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    • v.33 no.1
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    • pp.29-33
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    • 2009
  • The brilliant cresyl blue (BCB) has been used to select the developmental competent oocytes in pigs, goats and cows. Growing oocytes have a higher level of active glucose-6-phosphate dehydrogenase(G6PDH) compare to mature oocytes and are rarely stained compared to mature oocytes, because G6PDH converts BCB to colorless. First polar body extrusion regard as a guideline of meoisis completion. Selection of polar body extrude oocyte is more developmental competent to blastocyst than unselected. This study was conducted to compare the BCB test to the polar body extrusion on selection of developmental competent porcine oocytes for the production of blastocyst. Cumulus-Oocytes complex were exposed to 26uM BCB stain diluted in NCSU-23 for 90 min. There was no significant difference embryo development to blastocysts between BCB treated and not treated($19.58{\pm}1.99$ vs $18.75{\pm}2.27%$), which means there was no detrimental effect of BCB exposure to oocytes. Normal fertilization is not differed among treatment groups from 70.0 to 78.4% development to blastocyst, beside polyspermy did not. To compare two different selection methods, BCB test and polar body extrusion, evaluate the developmental competent of IVP embryos. BCB+PB+(blue stained and polar body extruded, $20.71{\pm}0.45%$) and BCB-PB+(colorless and polar body extruded, $20.04{\pm}l.29%$) groups are significantly (p<0.05) higher developed than those of BCB+PB-(blue stained and no polar body, $13.24{\pm}0.73%$) and BCB-PB-(colorless and no poladbody, $7.25{\pm}0.77%$). These results showed that selection of polar body extruded oocytes method is more efficient than that of BCB test.

Ground-based Observations of the Polar Region Space Environment at the Jang Bogo Station, Antarctica

  • Kwon, Hyuck-Jin;Lee, Changsup;Jee, Geonhwa;Ham, Young-Bae;Kim, Jeong-Han;Kim, Yong Ha;Kim, Khan-Hyuk;Wu, Qian;Bullett, Terence;Oh, Suyeon;Kwak, Young-Sil
    • Journal of Astronomy and Space Sciences
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    • v.35 no.3
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    • pp.185-193
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    • 2018
  • Jang Bogo Station (JBS), the second Korean Antarctic research station, was established in Terra Nova Bay, Antarctica ($74.62^{\circ}S$ $164.22^{\circ}E$) in February 2014 in order to expand the Korea Polar Research Institute (KOPRI) research capabilities. One of the main research areas at JBS is space environmental research. The goal of the research is to better understand the general characteristics of the polar region ionosphere and thermosphere and their responses to solar wind and the magnetosphere. Ground-based observations at JBS for upper atmospheric wind and temperature measurements using the Fabry-Perot Interferometer (FPI) began in March 2014. Ionospheric radar (VIPIR) measurements have been collected since 2015 to monitor the state of the polar ionosphere for electron density height profiles, horizontal density gradients, and ion drifts. To investigate the magnetosphere and geomagnetic field variations, a search-coil magnetometer and vector magnetometer were installed in 2017 and 2018, respectively. Since JBS is positioned in an ideal location for auroral observations, we installed an auroral all-sky imager with a color sensor in January 2018 to study substorms as well as auroras. In addition to these observations, we are also operating a proton auroral imager, airglow imager, global positioning system total electron content (GPS TEC)/scintillation monitor, and neutron monitor in collaboration with other institutes. In this article, we briefly introduce the observational activities performed at JBS and the preliminary results of these observations.