As cultured plant cells can grow in high oxidative stress conditions, they form an excellent system to study antioxidant mechanisms and the mass production of antioxidants. Oxidative stress is a major cause of damage in plants exposed to various types of environmental stress, including heavy metals, such as cadmium (Cd). Heavy metal accumulation can interfere with many cell functions and plant growth. To evaluate the contribution of oxidative stress to Cd-induced toxicity, cultured sweetpotato (Ipomoea batatas) cells were treated with increasing concentrations of Cd (0, 10, 25, and 50 μM) and cultured further. Cell growth was significantly inhibited by 25 and 50 μM of Cd, and the total protein content increased with 50 μM of Cd. Additionally, the activity of peroxidase (POD) and ascorbate peroxidase (APX), antioxidant enzymes that remove hydrogen peroxide (a reactive oxygen species), increased in the cells after treatment with 50 μM of Cd. The expression analysis of POD, APX, and peroxiredoxin (PRX) isolated from sweetpotato cultured cells in a previous study revealed the differential expression of POD in response to Cd. In this study, the expression levels of several acidic POD (swpa2, swpa3, and swpa4) and basal POD (swpb1, swpb2, and swpb3) genes were increased in Cd-treated cultured cells. These results indicate that Cd-mediated oxidative stress is closely linked to improved POD-mediated antioxidant defense capacity in sweetpotato suspension-cultured cells.
Song, Cheon Young;Lee, Dong Chul;Park, In Tae;Park, Wha Soon;Hong, Seung Min
FLOWER RESEARCH JOURNAL
/
v.17
no.4
/
pp.285-290
/
2009
This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of three-year-old cactus of potted Mammillaria goldii. M. theresae, and M. pseudopeclinata. Plant height and width of them was 2.5~2.8 cm, and 2.8~3.1 cm, and seed formation of Mammillaria goldii and M. theresae had internal body taking130 to 135 days to ripening seeds, however, M. pseudopectinata had externals taking just 26 days to ripening seeds. The mean seeded pod from April to June of M. goldii was 50.0% and the number of seeds in a pod was 25.3. In M. theresae, the mean seeded pod from April to June was 57.3% and the number of seeds in a pod was 35.0. However, the highest rate of seeded pod on May of M. pseudopectinata was 75.0% and the number of seed in a pod was 66.0. The sib crossing was much more ripening seed fertilization than that of self cross ing in the Mammillaria. The self crossing was formed from 12.0% to 20.0% of seeded pod, from 12.0 to 16.0 number of seed in a pod. However, sib crossing obtained 58.0% of seeded pod, 30 seeds in a pod of M. goldii, 65.0% seeded pods and 40 seeds in a pod of M. theresae, and 75.0% seeded pod, 68 seeds in a pod of M. pseudopectinala as the highest of them.
Song, Cheon Young;Lee, Dong Chul;Park, In Tae;Cho, Chang Hui;Hong, Seung Min
FLOWER RESEARCH JOURNAL
/
v.17
no.4
/
pp.291-296
/
2009
This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of four-year-old seedling cactus of potted Astrophytum asterias 'Nudum', A. asterias 'Super Kabuto', and A. myriostigma 'Nudum'. Plant height and width of Astrophytum asterias 'Nudum' and A. asterias 'Super Kabuto' was 5.3 to 5.5 cm, and 2.5 cm. However, plant height and width of A. myriostigma 'Nudum' was 7.6 cm and 5.6 cm as divided into 8 sections of body. In A. asterias 'Nudum', the mean seeded pod from April to September was 37.0% and the number of seeds in a pod was 43.0. In A. asterias 'Super Kabuto', the mean seeded pod from April to September was 22.5% and the number of seeds in a pod was 26.2. However, the mean seeded pod of A. myriostigma 'Nudum' from April to September was 32.3% and the number of seeds in a pod was 57.4. Especially, the seeded pod and the number of seeds in a pod of A. asterias 'Nudum' in July was the highest, 72.0% and 66.0. The sib crossing obtained much more seeded pods and the number of seeds per pod than that of selfing in the Astrophytum. The self crossing was formed from 9.0 to 20.0% of seeded pod, and 12.0 to 16.0 seeds in a pod. However, sib crossing was formed 60.0% seeded pod and 68.0 seeds in a pod on July of A. myriostigma 'Nudum'.
Transactions on Electrical and Electronic Materials
/
v.17
no.6
/
pp.341-350
/
2016
The current study is dedicated to design a novel coordinated controller to effectively increase power system rotor angle stability. In doing so, the coordinated design of an AVR (automatic voltage regulator), PSS2B, and TCSC (thyristor controlled series capacitor)-based POD (power oscillation damping) controller is proposed. Although the recently employed coordination between a CPSS (conventional power system stabilizer) and a TCSC-based POD controller has been shown to improve power system damping characteristics, neglecting the negative impact of existing high-gain AVR on the damping torque by considering its parameters as given values, may reduce the effectiveness of a CPSS-POD controller. Thus, using a technologically viable stabilizer such as PSS2B rather than the CPSS in a coordinated scheme with an AVR and POD controller can constitute a well-established design with a structure that as a high potential to significantly improve the rotor angle stability. The design procedure is formulated as an optimization problem in which the ITSE (integral of time multiplied squared error) performance index as an objective function is minimized by employing an IPSO (improved particle swarm optimization) algorithm to tune adjustable parameters. The robustness of the coordinated designs is guaranteed by concurrently considering some operating conditions in the optimization process. To evaluate the performance of the proposed controllers, eigenvalue analysis and time domain simulations were performed for different operating points and perturbations simulated on 2A4M (two-area four-machine) power systems in MATLAB/Simulink. The results reveal that surpassing improvement in damping of oscillations is achieved in comparison with the CPSS-TCSC coordination.
Kim, Young-Rok;Park, Eun-Seo;Park, Sang-Young;Choi, Kyu-Hong;Hwang, Yoo-La;Kim, Hae-Yeon;Lee, Byoung-Sun;Kim, Jae-Hoon
Journal of Astronomy and Space Sciences
/
v.26
no.1
/
pp.89-98
/
2009
In this study, the YLPODS (Yonsei Laser-ranging Precision Orbit Determination System) is developed for POD using SLR (Satellite Laser Ranging) NP (Normal Point) observations. The performance of YLPODS is tested using SLR NP observations of TOPEX/POSEIDON and CHAMP satellite. JPL's POE (Precision Orbit Ephemeris) is assumed to be true orbit, the measurement residual RMS (Root Mean Square) and the orbit accuracy (radial, along-track, cross-track) are investigated. The validation of POD using GPS (Global Positioning System) raw data is achieved by YLPODS performance and highly accurate SLR NP observations. YGPODS (Yonsei GPS-based Precision Orbit Determination System) is used for generating GPS based precise orbits for TOPEX/POSEIDON. The initial orbit for YLPODS is derived from the YGPODS results. To validate the YGPODS results the range residual of the first adjustment of YLPODS is investigated. The YLPODS results using SLR NP observations of TOPEX/POSEIDON and CHAMP satellite show that the range residual is less than 10 cm and the orbit accuracy is about 1 m level. The validation results of the YGPODS orbits using SLR NP observations of the TOPEX/POSEIDON satellite show that the range residual is less than 10 cm. This result predicts that the accuracy of this GPS based orbits is about 1m level and it is compared with JPL's POE. Thus this result presents that the YLPODS can be used for POD validation using SLR NP observations such as STSAT-2 and KOMPSAT-5.
This paper describes the development of an autonomous system for the precise orbit determination (POD) using GPS raw data. Orbit processing requiring the orbit determination (OD) accuracy of 1m ($1{\sigma}$) or sub-meter is relatively complicated comparing to that of more than several meters. The architecture of the developed system for processing POD automatically and the test results of it were presented. The implemented system is able to be used to the flight dynamics system of the satellite mission control system and moreover can be applied to the multi-satellite POD system by means of incorporating with the automated operational orbit processing system (i.e., Kgs automated Operational Orbit Processing System, KOOPS), which was already developed by the authors.
Kim, Moon-Chan;Park, Warn-Gyu;Chun, Ho-Hwan;Jung, Un-Hwa
International Journal of Naval Architecture and Ocean Engineering
/
v.2
no.1
/
pp.1-13
/
2010
A comparative study between a computation and an experiment has been conducted to predict the performance of a Pod type waterjet for cm amphibious wheeled vehicle. The Pod type waterjet has been chosen on the basis of the required specific speed of more than 2500. As the Pod type waterjet is an extreme type of axial flow type waterjet, theoretical as well as experimental works about Pod type waterjets are very rare. The main purpose of the present study is to validate and compare to the experimental results of the Pod type waterjet with the developed CFD in-house code based on the RANS equations. The developed code has been validated by comparing with the experimental results of the well-known turbine problem. The validation also extended to the flush type waterjet where the pressures along the duct surface and also velocities at nozzle area have been compared with experimental results. The Pod type waterjet has been designed and the performance of the designed waterjet system including duct, impeller and stator was analyzed by the previously mentioned m-house CFD Code. The pressure distributions and limiting streamlines on the blade surfaces were computed to confirm the performance of the designed waterjets. In addition, the torque and momentum were computed to find the entire efficiency and these were compared with the model test results. Measurements were taken of the flow rate at the nozzle exit, static pressure at the various sections along the duct and also the nozzle, revolution of the impeller, torque, thrust and towing forces at various advance speed's for the prediction of performance as well as for comparison with the computations. Based on these measurements, the performance was analyzed according to the ITTC96 standard analysis method. The full-scale effective and the delivered power of the wheeled vehicle were estimated for the prediction of the service speed. This paper emphasizes the confirmation of the ITTC96 analysis method and the developed analysis code for the design and analysis of the Pod type waterjet system.
Journal of the Computational Structural Engineering Institute of Korea
/
v.37
no.1
/
pp.9-16
/
2024
Using the proper orthogonal decomposition (POD) based intrusive reduced order model (ROM), the total degrees of freedom of the structural system can be significantly reduced and the critical time step satisfying the conditional stability increases in the explicit time integrations. In this study, therefore, the changes in the critical time step in the explicit time integrations are investigated using both the POD-ROM and Voronoi-cell lattice model (VCLM). The snapshot matrix is composed of the data from the structural response under the arbitrary dynamic loads such as seismic excitation, from which the POD-ROM is constructed and the predictive capability is validated. The simulated results show that the significant reduction in the computational time can be achieved using the POD-ROM with sufficiently ensuring the numerical accuracy in the seismic analyses. In addition, the validations show that the POD based intrusive ROM is compatible with the Voronoi-cell lattice based explicit dynamic analyses. In the future study, the research results will be utilized as an elemental technology for the developments of the real-time predictive models or monitoring system involving the high-fidelity simulations of structural dynamics.
Kim, Songhyon;Cho, Donghyurn;Lee, Sanghyun;Kim, Jongbum;Choi, Taekyu;Lee, Seungha
Journal of Aerospace System Engineering
/
v.15
no.6
/
pp.42-49
/
2021
To develop a high-resolution electro-optical/infrared (EO/IR) payload to be mounted on a high-speed and performance fighter aircraft in an external POD for acquiring daytime and nighttime image information on tactical targets, simulations, including flight environments and maneuvers, should be performed. Such simulations are pertinent to predicting the performance of several variables, such as aerodynamic force and inertia load acting on the payload. This paper describes the development of a flight data acquisition and analysis system based on flight simulation software (SW) for mission simulation of super-maneuverability fighter equipped with EO/IR payload. The effectiveness of the system is verified through comparison with actual flight data. The proposed flight data acquisition and analysis system based on FlightGear can be used as an M&S tool for system performance analysis in the development of the EO/IR payload.
Lee Sang Won;Kim Sang Ho;Kim Tae Heon;Kang Hyung Won;Lyu Yeoung Su
Journal of Physiology & Pathology in Korean Medicine
/
v.18
no.2
/
pp.507-516
/
2004
Alzheimer's disease(AD) is a geriatric dementia that is widespread in old age. In the near future AD will be the commom disease in public health service. Although a variety of oriental presciptions in study POD(Polygala tenuifolia extracted from dichlorometan) have been traditionally utilized for the treatment of AD, their pharmacological effects and action mechanisms have not yet fully elucidated. It has been widely believed that AP peptide divided from APP causes apoptotic neurotoxicity in AD brain. However, recent evidence suggests that CT105, carboxy terminal 105 aminoacids peptide fragment of APP, may be an important factor causing neurotoxicity in AD. SK-N-SH cells expressed with CT105 exhibited remarkable apoptotic cell damage. Based on morphological observations by phase contrast microscope and NO formation in the culture media, the CT105-induced cell death was significantly inhibited by POD. In addition, AD is one of brain degeneration disease. So We studied on herbal medicine that have a relation of brain degeneration. From old times, In Oriental Medicine, PO water extract has been used for disease in relation to brain degeneration. We were examined by ROS formation, neurite outgrowth assay and DPPH scravage assay. Additionally, we investigated the association between the CT105 and neurite degeneration caused by CT105-induced apoptotic response in neurone cells. We studied on the regeneratory and inhibitory effects of anti-Alzheimer disease in pCT105-induced neuroblastoma cell lines by POD. Findings from our experiments have shown that POD inhibits the synthesis or activities of CT105, which has neurotoxityies and apoptotic activities in cell line. In addition, treatment of POD(>50 ㎍/㎖ for 12 hours) partially prevented CT(105)-induced cytotoxicity in SK-N-SH cell lines, and were inhibited by the treatment with its. POD(>50 ㎍/㎖ for 12 hours) repaired CT105-induced neurite outgrowth when SK-N-SH cell lines was transfected with CT105. As the result of this study, In POD group, the apoptosis in the nervous system is inhibited, the repair against the degerneration of Neuroblastoma cells by CT105 expression is promoted. Decrease of memory induced by injection of scopolamin into rat was also attenuted by POD, based on passive avoidance test. Taken together, POD exhibited inhibition of CT105-induced apoptotic cell death. POD was found to reduce the activity of AchE and induced about the CA1 in rat hippocampus. Base on these findings, POD may be beneficial for the treatment of AD.
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