• Proceedings of the Korea Crystallographic Association Conference
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• 2002.11a
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• pp.4-4
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• 2002

PDZ domains bind to short segments within target proteins in a sequence-specific fashion. GRIP/ABP family proteins contain six to seven PDZ domains and interact via its sixth PDZ domain (class Ⅱ) with the C-termini of various proteins, including liprin-α. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class Ⅱ PDZ domain and DZ-mediated multimerization, we determined the crystal structures of the GRIPI PDZ6 domain, alone and in complex with a synthetic C-terminal octapeptide of human liprin-α, at resolutions of 1.5 Å and 1.8 Å, respectively. Remarkably, unlike other class Ⅱ PDZ domains, Ile736 at αB5 rather than conserved Leu732 at αB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix αB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr's side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.

• BMB Reports
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• v.44 no.6
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• pp.387-392
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• 2011

To investigate the molecular scavenging capabilities of the larvae of Hermetia illucens, two serine proteases (SPs) were cloned and characterized. Multiple sequence alignments and phylogenetic tree analysis of the deduced amino acid sequences of Hi-SP1 and Hi-SP2 were suggested that Hi-SP1 may be a chymotrypsin- and Hi-SP2 may be a trypsin-like protease. Hi-SP1 and Hi-SP2 3-D homology models revealed that a catalytic triad, three disulfide bonds, and a substrate-binding pocket were highly conserved, as would be expected of a SP. E. coli expressed Hi-SP1 and Hi-SP2 showed chymotrypsin or trypsin activities, respectively. Hi-SP2 mRNAs were consistently expressed during larval development. In contrast, the expression of Hi-SP1 mRNA fluctuated between feeding and molting stages and disappeared at the pupal stages. These expression pattern differences suggest that Hi-SP1 may be a larval specific chymotrypsin-like protease involved with food digestion, while Hi-SP2 may be a trypsin-like protease with diverse functions at different stages.

• KIEAE Journal
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• v.11 no.2
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• pp.83-90
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• 2011

This study has observed 12 parks (20 spots) in Seoul to know the number of users, gender, age groups for the units of 30 minutes from 06:00AM till 10:00PM. According to the systematic characteristic of the surveyed outdoor exercise place, the details of their users during the summer time were analyzed from the objective viewpoint. Major results of analysis are as follows: Firstly, in case of the riverside of the Han River 07:00AM marked a mini peak and 21:00PM reached the peak, and in case of sub-stream neighborhoods, 21:00PM showed only one peak. On the other hand, in case of neighborhood parks (including pocket park) no peak time was noticeable. Secondly, in case of the riverside of the Han River and the neighborhoods of sub-streams, people used the exercise equipment remarkably at night, and in case of neighborhood parks people used the exercise equipment a little bit more in the morning than in the afternoon. Thirdly, in case of nights in summer the riverside of the Han River was preferred by men, while the neighborhoods of sub-streams were preferred by women and the neighborhood parks showed no difference in the number of users among men and women. Fourthly, differently from men, women do not regard the exercise so seriously as men and their purpose of using outdoor exercise place is to use their leisure time mostly with their family members or neighbors, and women prefer light and comfortable exercises. Fifthly, in summer even those who like to exercise a lot like to exercise in the morning to avoid summer heat. Therefore, provision of shades in summer is very important.

• Journal of the Korean Institute of Landscape Architecture
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• v.23 no.1
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• pp.123-139
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• 1995

The megalopolis citizens are reevaluating the expectant value and the perception of leisure aceording to increasing the level of their incomes, The leisure of citizens is increasing in the aspect of quantity and quality of life nowadays In the site planning of leisure, the concrete understanding about people's choice of a leisure site will be the most important thing, not only for the aspect of improvement of the quality of life but also efficiencies of the land use The purposes of chi study are firstly, to find out the factors which are influenced on a choice of Outdoor leisure facilities in neighbourhood unit and to Compare the Characteristics Of Choice models between the three metropolitan areas, secondly, to predict a limitation of use areal according to the change of a needed time based on the metropolitan's standard choice model For the choice model establishment this research used Logit Model which has been used in the field of the traffic, the tourism and the economics. This research made the results which find out the influencing variables with needed times, the accessibility and the percentage of facilities. The lindtations of use areas come out the results which are predicted according to the change of needed times as a most influencing factors. The range of each preferred leisure facility is about 956 meter distant in the neighbourhood park about 644 meter distant in the pocket park about 604 meter distant in the recreation center, about 628 meter distant in the tennis court about 974 meter distant in the private hob by facility and about 528 meter distant in the library from the apartment unit The recreation center and the library are nearer facilities than the other facilities. But these facilities are surveyed to be more or less influenced by an interesting progran, a context of events and the level of useful facilities, etc.

• Journal of Microbiology and Biotechnology
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• v.29 no.2
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• pp.244-255
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• 2019

Xylose isomerase (XI; E.C. 5.3.1.5) catalyzes the isomerization of xylose to xylulose, which can be used to produce bioethanol through fermentation. Therefore, XI has recently gained attention as a key catalyst in the bioenergy industry. Here, we identified, purified, and characterized a XI (PbXI) from the psychrophilic soil microorganism, Paenibacillus sp. R4. Surprisingly, activity assay results showed that PbXI is not a cold-active enzyme, but displays optimal activity at $60^{\circ}C$. We solved the crystal structure of PbXI at $1.94-{\AA}$ resolution to investigate the origin of its thermostability. The PbXI structure shows a $({\beta}/{\alpha})_8$-barrel fold with tight tetrameric interactions and it has three divalent metal ions (CaI, CaII, and CaIII). Two metal ions (CaI and CaII) located in the active site are known to be involved in the enzymatic reaction. The third metal ion (CaIII), located near the ${\beta}4-{\alpha}6$ loop region, was newly identified and is thought to be important for the stability of PbXI. Compared with previously determined thermostable and mesophilic XI structures, the ${\beta}1-{\alpha}2$ loop structures near the substrate binding pocket of PbXI were remarkably different. Site-directed mutagenesis studies suggested that the flexible ${\beta}1-{\alpha}2$ loop region is essential for PbXI activity. Our findings provide valuable insights that can be applied in protein engineering to generate low-temperature purpose-specific XI enzymes.

• Journal of Periodontal and Implant Science
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• v.51 no.3
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• pp.163-178
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• 2021

Purpose: The aim of this study was to evaluate clinical factors affecting the longevity of fixed retainers and the influence of fixed retainers on periodontal health in periodontitis patients. Methods: In total, 52 patients with at least 2 years of follow-up after periodontal and orthodontic treatment were included in this study. After scaling and root planing, orthodontic treatment with fixed appliances or clear aligners was performed. Fixed retainers with twist-flex stainless steel wires were bonded to the palatal or lingual sides of anterior teeth. Changes in clinical parameters, including the plaque index, gingival index, calculus index (CI), probing pocket depth, and radiographic bone levels, were evaluated before bonding of fixed retainers and at a 12-month follow-up. Cumulative survival rates (CSRs) for retainer failure were evaluated according to sex, site, CI, stage of periodontitis, and the severity of the irregularity with the log-rank test and hazard ratios (HRs). Results: Twelve months after bonding of fixed retainers, improvements were observed in all clinical parameters except CI and radiographic bone gain. The overall CSR of the retainers with a CI <1 at the 12-month follow-up after bonding of fixed retainers was significantly higher than that of the retainers with a CI ≥1 at the 12-month follow-up (log-rank test; P<0.001). Patients with stage III (grade B or C) periodontitis had a higher multivariate HR for retainer failure (5.4; 95% confidence interval, 1.22-23.91; P=0.026) than patients with stage I (grade A or B) periodontitis. Conclusions: Although fixed retainers were bonded in periodontitis patients, periodontal health was well maintained if supportive periodontal treatment with repeated oral hygiene education was provided. Nonetheless, fixed retainer failure occurred more frequently in patients who had stage III (grade B or C) periodontitis or a CI ≥1 at 12-month follow-up after bonding of fixed retainers.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.9 no.7
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• pp.707-722
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• 2019

The concept of neighborhood parks has been used as the principle of forming a community in our society going through modern and contemporary times. The concept of neighborhood parks equipped with physical facilities has been discussed constantly regarding the roles and meanings of them. This study focuses on pocket parks which have been playing roles in improving environment through regeneration and forming regional communities based on that as neighborhood facilities. This author carried out field investigation on total 72 Hanpyeong Park and Hanpyeom Parks formed in the capital area and examined the significance of planning them and also their utility and residents and users' attachment to them through a survey. In this process, this researcher has been able to draw following conclusions. First, according to the demographic characteristics of users, there is significant difference found by groups in terms of their place attachment to the parks. This implies that difference in the major types of residences supplied these days influences their intention to experience ecological space. Second, according to their educational background, there is significant difference found by groups regarding their place attachment to the parks. Third, according to user behavior, there are differences found in their place attachment to the parks and also detailed differences found by groups in terms of the frequency of use and duration of stay.

• Journal of the Korean Geosynthetics Society
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• v.17 no.1
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• pp.75-83
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• 2018

This paper described a results of dynamic stability by using wheel tracking test and unconfined compression test, in order to evaluate behavior characteristics on the developed pocketable expansion material for emergency restoration of ground cavity. The wheel tracking test result showed that the settlement increment ratio of the recovered ground by the expansion material was decreased compared to the sandy ground in high load condition. That is, it was confirmed that the expansion material was able to restrain the settlement due to the material stiffness, and the same results were obtained for the dynamic stability evaluation results. From the results of unconfined compression test, the pocketable expansion material was found to be able to fully support load on the restored cavity.

• Journal of the Korean Society of Clothing and Textiles
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• v.33 no.8
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• pp.1227-1240
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• 2009

This study examines the actual conditions of toddler playwear preference survey for design and function. The study develops proper indoor and outdoor playwear for toddlers based on the analysis. A survey of 200 parents with toddlers between 2 and 6 years old and 120 teachers was conducted to find the conditions and needs for playwear along with the necessary design elements. Second, 6 designs including 3 designs for indoor playwear and 3 designs for outdoor playwear were made. These designs emphasized on soil contamination part, color, pattern, form, material, detail, and fasteners. Art play and cooking play are considered for indoor playwear and ways to decrease contamination on sleeve hem, elbow, chest, and knee were determined through the use of yellow, green, and blue colors. Applying a character and separate top with sleeves were determined. Light with breathable and waterproof fabric was determined. The correct amount of Velcro fasteners or buttons was determined for front fastening in addition a pocket was considered necessary as a detail part. The common design for both boys and girls along with a proper design for diverse play were determined. For outdoor playwear, water play, sand play, movement play, and ecology experience are considered and ways to decease contamination on hip, sleeve hem, and knee should be applied to the design as determined through the use of yellow, green, blue, and red colors. The demands for pattern, form, material, and details were similar to indoor playwear. Zipper and buttons for front fastening were determined. These final 6 playwear designs are presented using CAD WALK DESIGNWARE.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.1191-1195
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• 2006

Now days, heavy storm occur to be continue. It is hard to use before frequency based on flood discharge for decision that design water pocket structure. We need to estimation of frequency based on flood discharge on the important basin likely city or basin that damage caused by flood recurrence. In this paper flood discharge calculated by Clark watershed method and SCS synthetic unit hydrograph method about upside during each minute of among time distribution method of rainfall, Huff method choosing Bocheong Stream basin that is representative basin of International Hydrologic Project (IHP) about time distribution of rainfall that exert big effect at flood discharge estimate to research target basin because of and the result is as following. Relation between probability flood discharge that is calculated through frequency analysis about flood discharge data and rainfall - runoff that is calculated through outward flow model was assumed about $48.1{\sim}95.9%$ in the case of $55.8{\sim}104.0%$, SCS synthetic unit hydrograph method in case of Clark watershed method, and Clark watershed method has big value overly in case of than SCS synthetic unit hydrograph method in case of basin that see, but branch of except appeared little more similarly with frequency flood discharge that calculate using survey data. In the case of Critical duration, could know that change is big area of basin is decrescent. When decide time distribution type of rainfall, apply upside during most Huff 1-ST because heavy rain phenomenon of upsides appears by the most things during result 1-ST about observation recording of target area about Huff method to be method to use most in business, but maximum value of peak flood discharge appeared on Huff 3-RD too in the case of upside, SCS synthetic unit hydrograph method during Huff 3-RD incidental of this research and case of Clark watershed method. That is, in the case of Huff method, latitude is decide that it is decision method of reasonable design floods that calculate applying during all $1-ST{\sim}4-TH$.