• 생명과학회지
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• 제28권1호
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• pp.99-104
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• 2018

Streptococcus pneumoniae는 pneumolysin과 같은 병원성 인자를 가지고 있으며, 지역사회에서 전파되는 심각한 병원성균에 포함된다. Pneumolysin (PLY)은 콜레스테롤 의존적으로 세포막에 구멍을 형성하는 세포독소로서 백신의 주요한 표적 항원이다. PLY의 연구를 위하여 Streptococcus pneumoniae D39 균주에서 추출한 genomic DNA를 주형으로 하여 PCR을 시행하였다. 합성된 PLY 유전자 DNA를 pQE-30 vector에 삽입하고, E. coli M15에 형질전환 시킨 후 LB 배지에 IPTG를 첨가하여 PLY 단백질을 생산하였다. 재조합 단백질은 $Ni^{2+}$-agarose column을 사용하여 정제하였다. 또한, EGFP를 PLY C-말단에 부착한 융합단백질도 동일한 방법으로 클로닝하여 재조합 단백질을 생산하였다. 500 ng/ml 농도의 재조합 PLY는 1.0% 적혈구 현탁액을 100% 용혈시켰으며, 240 ng/ml 농도는 50% 용혈을 나타내었다. 그러나 재조합 PLY-EGFP는 용혈 활성이 전혀 나타나지 않았으나 형광현미경으로 관찰하였을 때 적혈구 막에 결합되어 있었다. 즉, EGFP의 PLY C-말단 부착은 PLY의 세포막 결합능은 유지시켰으나 용혈기능은 방해하였다. PLY C-말단은 용혈기능에 아주 중요한 영역이며, 세포막 결합은 PLY의 다른 영역이 보다 중요하게 작용할 것으로 추측된다. 따라서, 육안으로 관찰이 가능한 결합능은 가졌으나 용혈 기능이 결여된 PLY-EGFP를 대조군으로 활용함으로써 두 재조합 단백질은 폐렴 유발에 있어서 PLY 작용 연구에 사용될 수 있을 것으로 기대된다.

• Journal of Mechanical Science and Technology
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• 제16권3호
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• pp.292-301
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• 2002

Ultrasound waves interact strongly with the orientation and sequence of the plies in a layup when propagating in the thickness direction of composite laminates. Also the layup orientation greatly influences its properties in a composite laminate. If the layup orientation of a ply is misaligned, it could result in the part being rejected and discarded. Now, most researchers cut a small coupon from the waste edge and use a microscope to optically verify the ply sequences on important parts. This may add a substantial cost to the production since the test is both labor intensive and performed after the part is cured. A nondestructive technique would be very beneficial, which could be used to test the part after curing and requires less time than the optical test. Therefore we have developed, reduced, and implemented a novel ply-by-ply vector decomposition model for composite laminates fabricated from unidirectional plies. This model decomposes the transmission of a linearly polarized ultrasound wave into orthogonal components through each ply of a laminate. High probability is found, by comparisons between the model and tests, in characterizing cured layups of the laminates by using the proposed method.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집A
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• pp.401-406
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• 2001

When propagating the thickness direction of composite laminates ultrasound waves interacts strongly with the orientation and sequence of the plies in a layup. Also the layup orientation greatly influences its properties in a composite laminate. If one ply of the layup orientation is misaligned, it could result in the part being rejected and discarded. Now, most researchers cut a small coupon from the waste edge and use a microscope to optically verify the ply sequences on important parts. Those may add a substantial cost to the product since the test is both labor hard and performed after the part is cured. A nondestructive technique would be very beneficial, which could be used to test the part after curing and require less time than the optical test. Therefore we have developed, reduced, and implemented a novel ply-by-ply vector decomposition model for composite lam mates fabricated from unidirectional plies. This model decomposes the transmission of a linearly polarized ultrasound wave into orthogonal components through each ply of a laminate. It is found that a high probability shows between the model and tests developed in characterizing cured layups of the laminates.

• Journal of Mechanical Science and Technology
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• 제18권1호
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• pp.65-73
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• 2004

Composites are a material class for which nondestructive material property characterization is as important as flaw detection. Laminates of fiber reinforced composites often possess strong in-plane elastic anisotropy attributable to the specific fiber orientation and layup sequence when waves are propagating in the thickness direction of composite laminates. So the layup orientation greatly influences its properties in a composite laminate. It could result in the part being .ejected and discarded if the layup orientation of a ply is misaligned. A nondestructive technique would be very beneficial, which could be used to test the part after curing and requires less time than the optical test. Therefore a ply-by-ply vector decomposition model has been developed, simplified, and implemented for composite laminates fabricated from unidirectional plies. This model decomposes the transmission of a linearly polarized ultrasound wave into orthogonal components through each ply of a laminate. Also in order to develop these methods into practical inspection tools, motorized system have been developed for different measurement modalities for acquiring ultrasonic signals as a function of in-plane angle. It is found that high probability shows between the model and tests developed in characterizing cured layups of the laminates.

• 비파괴검사학회지
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• 제23권6호
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• pp.566-576
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• 2003

Carbon-fiber reinforced plastics (CFRP) composite laminates often possess strong in-plane elastic anisotropy attributable to the fiber orientation and layup sequence. The layup orientation thus greatly influences its properties in a composite laminate. It could result in the part being rejected or discarded if the layup orientation of a ply is misaligned. A nondestructive technique would be very beneficial, which could be used to test the part after curing and to require less time than the optical test. In this paper, ultrasonic scanners were set out for different measurement modalities for acquiring ultrasonic signals as a function of in-plane azimuthal angle. The motorized scanner was built first for making transmission measurements using a pair of normal-incidence shear wave transducers. Another scanner was then built fer the acousto-ultrasonic configuration using contact transducers. A ply-by-ply vector decomposition model has been developed, simplified, and implemented for composite laminates fabricated from unidirectional plies. We have compared the test results with model data. It is found that strong agreement are shown between tests and the model developed in characterizing cured layups of the laminates.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2001년도 춘계학술대회 논문집
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• pp.744-747
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• 2001

This paper shows shear wave behavior of CFRP composite laminates as a polar grid form to evaluate vibration pattern of ultrasonic transducers, which gives measured modelling fundamental contents of nondestructive evaluation. Polarized direction can be obtained by using a c-scanner and sensitivity of transducers is founded when using through-transmission method of two transducers. And modelling of vector decomposition is presented based on ply-to-ply method to apply practicable nondestructive evaluation of CFRP laminate lay up. This modelling decomposes the transmission of linearly polarized wave into orthogonal components through each ply of a laminate. It is found that a high provable shows between the model and experimental developed in characterizing layup of CFRP composite laminates.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 추계학술대회 논문집
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• pp.892-895
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• 2002

In this study, to grasp the effect of vibration intensity fur the laminated composite plate, the two-dimension plate was shaken by a harmonic point excitation with the natural frequency using the finite element method. As the result, it shows that the vibration intensity according to the change of angle-ply is various and it flows to the direction of length rather than width in the plate. Also this paper represents those results to the vector flow.

• Structural Engineering and Mechanics
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• 제40권2호
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• pp.257-277
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• 2011

In this paper the geometrically nonlinear continuum plate finite element model, hitherto not reported in the literature, is developed using the total Lagrange formulation. With the layerwise displacement field of Reddy, nonlinear Green-Lagrange small strain large displacements relations (in the von Karman sense) and linear elastic orthotropic material properties for each lamina, the 3D elasticity equations are reduced to 2D problem and the nonlinear equilibrium integral form is obtained. By performing the linearization on nonlinear integral form and then the discretization on linearized integral form, tangent stiffness matrix is obtained with less manipulation and in more consistent form, compared to the one obtained using laminated element approach. Symmetric tangent stiffness matrixes, together with internal force vector are then utilized in Newton Raphson's method for the numerical solution of nonlinear incremental finite element equilibrium equations. Despite of its complex layer dependent numerical nature, the present model has no shear locking problems, compared to ESL (Equivalent Single Layer) models, or aspect ratio problems, as the 3D finite element may have when analyzing thin plate behavior. The originally coded MATLAB computer program for the finite element solution is used to verify the accuracy of the numerical model, by calculating nonlinear response of plates with different mechanical properties, which are isotropic, orthotropic and anisotropic (cross ply and angle ply), different plate thickness, different boundary conditions and different load direction (unloading/loading). The obtained results are compared with available results from the literature and the linear solutions from the author's previous papers.