• Korean Journal of Plant Resources
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• v.20 no.1
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• pp.73-78
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• 2007

Efficient pollination needs abundant fertile pollen in rose breeding. This study was performed to find out efficient staining methods for the detection of fertile pollen. Aceto-carmine and Alexander's stain gave similar results in terms of percentage of normal pollen. Fluorochromatic reaction (FCR) showed the lowest normal pollen percentage because FCR stained only fertile pollen while others stained cytoplasm. Toluidine blue O (TB) showed similar percentage of normal pollen to Aceto-carmine and Alexander's, but could not clearly distinguish the clustered abnormal pollens. Alexander's stain was easy and simple, but difficult to distinguish fertile and infertile pollen. FCR showed only fertile pollen. Alexander's stain showed approximate fertility and FCR showed exact pollen fertility.