• Title/Summary/Keyword: Plunging

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Structural characteristics of the Yecheon Shear Zone in the Pukhumyeon-Pyeongeunmyeon area, Gyeongsangbukdo, Korea (경상북도 북후면-평은면 지역에 발달된 예천전단대의 구조적 특성)

Development of Cryopreservation Protocols through Droplet-vitrification and its Application to Vegetatively Propagated Crop Germplasm (영양체 유전자원의 작은방울-유리화법에 의한 초저온동결보존 실용화기술개발)

  • Kim, Haeng-Hoon;Yi, Jung-Yoon;No, Na-Young;Cho, Gyu-Taek;Yoon, Mun-Sup;Baek, Hyung-Jin;Kim, Chung-Kon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2010.05a
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    • pp.12-12
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    • 2010
  • We developed droplet-vitrification protocol, a combination of droplet-freezing and solution-based vitrification, and applied to germplasm collections of garlic, potato, lily as well as cell lines, including hairy roots, somatic embryos. To establish a garlic cryobank, four Korean garlic field collections at Danyang, Suwon, Mokpo and Namhae were cryopreserved last five years. The protocol applied consisted of preculture for 3-4 days at $10^{\circ}C$ on solid MS medium with 0.3M sucrose, loading for 40 min in liquid medium with 35% PVS3, dehydration with PVS3 for 150 min, cooling in $5{\mu}l$ droplets of PVS3 placed on aluminum foil strips by dipping these strips in liquid nitrogen, warming them by plunging the foil strips into pre-heated($40^{\circ}C$) 0.8M sucrose solution for 30s. A total of over 900 accessions of garlic were stored in liquid nitrogen for long-term conservation using unripe inflorescences, cloves or bulbils. Twelve alternative plant vitrification solutions were designed by modifying cryoprotectant concentrations from the original PVS2 and PVS3. The results suggest that PVS2-based vitrification solutions with increased glycerol and sucrose and/or decreased DMSO and EG concentrations can be applied for medium size explants which are tolerant to chemical toxicity and moderately sensitive to osmotic stress. PVS3 and variants can be used widely when samples are heterogeneous, of large size and/or very sensitive to chemical toxicity and tolerant to osmotic stress.

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Comparison between Two Cryo-devices for Vitrification of Immature Oocytes of Indigenous Zebu Cows in Bangladesh

  • Choudhury, Sk Mohiuddin;Bhuiyan, Mohammad Musharraf Uddin;Rahman, Mohammad Moshiur;Rahman, Md. Masudur;Sharif, Md. Newaz;Bhattacharjee, Jayonta;Bari, Farida Yeasmin;Juyena, Nasrin Sultana
    • Journal of Embryo Transfer
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    • v.32 no.4
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    • pp.311-317
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    • 2017
  • Cryopreservation of oocytes by vitrification technique may contribute a lot in the field of reproductive biotechnology. The objectives of the present study were to evaluate the effectiveness of two cryo-devices for vitrification of immature oocytes of indigenous zebu cows. Slaughter house derived immature cumulus-oocyte-complexes (COCs) of cows were vitrified using 15% dimethyl sulphoxide (DMSO) as cryoprotective agent (CPA) with 0.5 mol sucrose in TCM 199 supplemented with 20% FBS. Vitrification of COCs was completed after immediate plunging of COCs loaded cryotop or French mini straw into the liquid nitrogen ($LN_2$). Then the COCs containing cryotop or French mini straws were warmed in 0.25 mol sucrose and 20% FBS supplemented TCM 199 followed by in vitro culture in $50{\mu}l$ droplets of bicarbonate buffered TCM 199 supplemented with 10% FBS, pyruvate, FSH and oestradiol for 24 hrs at $39^{\circ}C$ with 5% CO2 in humidified air. After maturation culture, oocytes were denuded and examined under inverted microscope for presence of polar body as the indication of maturation. Denuded oocytes were also stained by whole mount technique using 1% orcein to examine the maturation by presence of MII chromosomes. The in vitro maturation rate was significantly (p<0.05) higher in oocytes vitrified and warmed using crytop ($47.1{\pm}6.9%$) than that of French mini straw ($15.9{\pm}12.5%$). Moreover, in vitro maturation rate was significantly (p<0.05) highe r in control oocytes (not vitrified) ($84.5{\pm}14.2%$) than that of vitrified oocytes. In conclusion, cryotop is better than French mini straw as cryo-device for vitrification of bovine immature oocytes.

Dynamics of High Turbid Water Caused by Heavy Rain of Monsoon and Typhoon in a Large Korean Reservoir (Andong Reservoir) (인공호에서 몬순과 태풍 강우에 의한 고탁수층의 이동과 소멸특성)

  • Park, Jung-Won;Shin, Jae-Ki;Lee, Hee-Moo;Park, Jae-Chung
    • Korean Journal of Ecology and Environment
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    • v.38 no.1 s.110
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    • pp.105-117
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    • 2005
  • During the period of heavy rain from 2002 to 2004, the characteristics of the inflow, temporal and spatial fluctuations of high turbid water according to thermal stratification were studied on the Andong Reservoir which is the largest artificial lake in the Nakdong River basin, Korea. Thermal stratification was formed in June. Its structure determined to the pathway of inflowing turbid water and has affected by the transportation of high turbid water. Regardless of the time and amount of inflow, the high turbid water showed the shape of underflow at the riverine zone, separated from the bottom at the transition zone and moved to the lacustrine zone with the shape of density current. The plunging point depended on the time and amount of inflow. The distributions of thermal stratification and DO concentrations were changed by inflowing discharge. Two thermoclines and minimum DO layers were found out existing at metalimnion in a specific time, respectively. The layer of high turbid water which formed with the thickness of 20 m at the maximum below the depth of 15 m moved toward dam. Not settled to the bottom, the newly formed layer was discharged through the intake-outlet and dispersed into all layers by the circulation in the fall.

Establishment of the Convenient Boar Semen Freezing Method and Assessment of Viability in Frozen/Thawed Boar Semen (돼지 정액의 간편 동결 방법 확립과 동결 정액의 융해 후 생존성 평가)

  • Kim Seong-Kon;Jang Hyun-Yong;Park Dong-Heon;Park Chun-Keun;Cheong Hee-Tae;Kim Choung-Ik;Yang Boo-Keun
    • Reproductive and Developmental Biology
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    • v.30 no.1
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    • pp.59-64
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    • 2006
  • This study was conducted to establish a convenient freezing method of boar semen. Boar semen was cooled until $5^{\circ}C$ for 3 hrs using cell freezer and loaded into straws. Semen straws were frozen in different steps in strofoam box filled with $LN_2$. Highest sperm viability (54.0%) was obtained by 1-step freezing(holding at 10 cm height from the surface of $LN_2$ for 10 min). Sperm viability increased by holding at $-102^{\circ}C$ for 10min (74.0%, P<0.05). In thawing regime, sperm viability was significantly higher in $37^{\circ}C$ group than in $52^{\circ}C$ group. The sperm characteristics did not differ between 1-step and 3-step. After IVF using frozen-thawed boar semen, developmental rate of embryos to the morula+blastocyst stage was in 1-step freezing group than that of 3-step freezing group (27.5 vs 14.7%, P<0.05). The result shows that the 1-step freezing with holding at $-102^{\circ}C$ for 10min before plunging into $LN_2$ is a convenient and easy freezing method for boar semen.

Using Dark Tourism Study on Culture and Tourism of industrialization - Focusing on Honam righteous army - (다크 투어리즘을 활용한 문화관광 산업화 방안에 관한 연구 - 한말 호남 의병사(義兵史)를 중심으로 -)

  • Kim, Hyun-Cheol;Yoo, Jun-Ho;Ha, Kyu-Soo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.6 no.1
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    • pp.59-81
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    • 2011
  • The 21st century, the creation and utilization of knowledge and information are the core of all economic activity, knowledge-based economy is plunging into. Culture can not live without knowing the age of culture and national spirit of a nation's cultural assets. Closed for other cultures, countries can develop a culture that is neither marked by imitation and import of cultural industries can never be developed. Creative and diverse culture of its own cultural code, evenly balanced mix of social culture is really the cultural and creative culture. Central government and local government-led growth in the local economy in terms of competitive cultural industry development policy has been carried out. In general, a country or society, culture, industry, the creation of a unique cultural and Planning on the basis of information deliquescent recreated a typical high-risk high returns to pay the venture industry and the nature of the creative industries and the venture industry a unique mix of industrial Characteristic is that it has. These times and the industry can contribute to the flow of valuable cultural assets and a history of our righteous living and exercise will be a source of resources.

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A Building of Investment Decision Model for Improving Profitabilty of Tramper Shipping Business (해운산업 수익성 제고 투자의사결정 모델구축에 관한 연구 - 부정기선 영업을 중심으로 -)

  • Kim, Weon-Jae
    • Journal of Korea Port Economic Association
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    • v.27 no.2
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    • pp.297-311
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    • 2011
  • This paper deals with a strategic investment decision model for improving investment profit in shipping industry. Despite the quantitative expansion of Korean shipping business, many shipping firms have suffered financial difficulties due to financial and operating risks that result from the characteristics of capital-intensive business as well as of volatility of shipping markets. As a result, managers in charge of making an investment decision, particularly in tramper business sector, are required to take both financial and operating risk factors into consideration. Put it differently, managers are strongly recommended to avoid these risks by ship asset play; buy-low and sell-high, which results in considerable capital gain and cost reduction. In addition, managers in shipping industry are also recommended to consider the ship chartering investment alternative when the freight markets show extreme volatility as the case of 2008 triggered by sub-prime mortgage financial crisis in USA. For example, the BDI suffered plunging down from 1000 in 2008 to 100 in 2010. Consequently, the 4th largest shipping company in Korea, DAEHAN Shipping Co., has collapsed primarily due to excessive tonnage expansion during the peak time of bulk market. In sum, the strategic investment decision model, suggested in this paper, is designed to include such factors as capital gain by asset play, timely chartering for alternative shipping service, and optimization of operating profit by tonnage adjustment in accordance with change in the shipping markets concerned.

Development Pattern and Ductile Deformation of the Sancheong Fe-Ti Mineralized Zone, Korea (산청 철-티탄 광화대의 발달양상과 연성변형)

  • Ryoo, Chung-Ryul;Kim, Jong-Sun;Son, Moon;Koh, Sang-Mo;Lee, Han Yeang;Kang, Ji-Hoon
    • The Journal of the Petrological Society of Korea
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    • v.22 no.2
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    • pp.209-217
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    • 2013
  • Fe-Ti ore bodies occur in the western part of the Sancheong anorthosites around Banggok-ri, Sancheong, Korea. Within ore bodies, a several centimetric size of anorthositic breccia are enclaved by ore-bearing mafic part and deformed strongly as a sigmoidal form by ductile shearing. The ore bodies have a general N-S trending foliations with westward dipping directions. The foliation developed in the ore bodies cut the foliation in anorthosites. The stretching lineations are well developed in the foliated plane of the ore bodies, showing ENE-trending with gentle plunging angle to the ESE direction. The sigmoidal patterns of anorthositic breccia in the ore bodies indicates the top-to-the-eastnortheastward shearing. Thus, in this study area the relationship between the geometric pattern and the ductile deformation is an important fact to understand the Sancheong Fe-Ti mineralized zone, Korea.

Stratigraphy and Geological Structure of the Northwestern Okcheon Metamorphic Belt Near the Chungju Area (충주지역 북서부 옥천변성대의 층서 및 지질구조)

  • Ryu, In-Chang;Kim, Tae-Hoon
    • Economic and Environmental Geology
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    • v.42 no.1
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    • pp.9-25
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    • 2009
  • The Northwestern Okcheon Metamorphic Belt in the Chungju area consists of the Munjuri Formation, the Daehyangsan Quartzite, the Hyangsanri Dolomite, and the Gyemyeongsan Formation, but the stratigraphy is still controversial. For a stratigraphic study, detailed stratigraphic sections were measured in two locations and mapping was carried out in the study area. The Munjuri Formation and the Daehyangsan Quartzite changed gradually in north and south section, but bedding parallel faults have developed in the boundary between two formations. The Daehyangsan Quartzite and the Hyangsanri Dolomite are conformable. Fault have developed in boundary between the Hyangsanri Dolomite and the Gyemyeongsan Formation. As a result of mapping in the study area, folding was recognized with $41^{\circ}/280^{\circ}$ plunging axis in the north part of the study area. Therefore, the bedding-parallel faults in the boundary might have occurred resulting from a layer parallel slip during the folding as well as the thrust. These results from this study and previous studies indicate that bedding-parallel faults in boundary between the Munjuri Formation and the Daehyangsan Quartzite are caused by a layer parallel slip during the folding. The fault between the Hyangsanri Dolomite and the Gyemyeongsan Formation is considered as a thrust fault, thereby the uppermost Gyemyeongsan Formation is placed under the Munjuri Formation. However the Gyemyeongsan Formation and the Munjuri Formation have similar age and rock composition. Hence, the Gyemyeongsan Formation is considered as an equivalent one with the Munjuri Formation. Therefore, the stratigraphy of Northwestern Okcheon Metamorphic Belt consists of the Gyemyeongsan/ Munjuri formations, the Daehyangsan Quartzite, and the Hyangsanri Dolomite in ascending order.

Ductile Shear Deformation around Jirisan Area, Korea (지리산 일대의 연성전단변형)

  • Ryoo, Chung-Ryul;Kang, Hee-Cheol;Lee, Sang-Won
    • The Journal of the Petrological Society of Korea
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    • v.28 no.2
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    • pp.53-69
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    • 2019
  • In the Jirisan area of the Yeongnam Massif, Korea, several ductile shear zones are developed within Precambrian gneiss complex (Jirisan metamorphic rock complex). The ductile shear zones have a general NS- and NNE-striking foliation with westward dipping directions. The foliation developed in the shear zones cut the foliation in gneiss complex. The stretching lineations are well developed in the foliated plane of the shear zone, showing ENE-trend with gentle plunging angle to the ESE direction. Within shear zone, several millimetric to centimetric size of porphyroclasts are deformed strongly as a sigmoid form by ductile shearing. The sigmoid patterns of porphyroclasts in the shear zones indicate the dextral shearing. The spatial distribution of ductile shear zone is characterized by the dominant NS- and NNE-striking dextral sense in the central and eastern regions respectively. In the western part, it develops in NE-striking dextral sense which is the general direction of the Honam shear zone. The U-Pb concordant ages obtained from the two samples, the strongly sheared leucocratic gneiss, are $1,868{\pm}3.8Ma$ and $1,867{\pm}4.0Ma$, respectively, which are consistent with the U-Pb ages reported around the study area. We supposed that the ductile shearing in the study area is occurred about 230~220 Ma during late stage of the continental collision around Korea and is preceded by granitic intrusion related to subduction during 260~230 Ma, which are supported by compiling the age data from sheared gneiss, deformed mafic dyke intruded gneiss complex, and non-deformed igneous rocks.