• 제목/요약/키워드: Plk1

검색결과 29건 처리시간 0.019초

상황버섯, Phellinus linteus 계통과 Phellinus baumii의 항 혈소판 응집 효과 (Inhibitory effects of Sanghwang mushrooms, Phellinus linteus strains and Phellinus baumii, on platelet aggregation)

  • 강희완;이만휘;여운형
    • 한국버섯학회지
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    • 제15권3호
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    • pp.124-128
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    • 2017
  • 국내 상황버섯 재배 종인 Phellinus baumii (PB, Jangsu sanghwang), P. linteus (PLK, Korea Sangwhang), 그리고 P. linteus HN00k9 (PLH) 자실체의 methanol 추출물의 혈소판 응집 억제와 ATP release 감소효과를 조사하였다. PB, PLK, PLH methanol 추출물을 전 처리하고 혈소판 응집촉진물인 collagen (2.5 ug/ml), ADP (10 uM), thrombin (0.1 U/ml) 처리에 50ug/ml에서 200 ug/ml 농도에서 50%에서 95%의 혈소판응집억제 효과가 나타났으며 ATP release 감소효과는 200 ug/ml 농도에서 ADP(10 uM) 처리 혈소판에서 60%에서 50%로 나타났으며 이는 Phospholipase C의 inhibitor인 U73122 (4 uM)과 calcium chelator인 EDTA (1 mM)에 비하여 20%이상 높게 나타났다. 본 결과로부터 상황버섯 PB, PLK, PLH 메탄올 추출물은 심혈관계질병을 예방 하는 유용한 소재로 활용 가능 할 것으로 사료 된다.

암의 중심체 증폭 이해를 통한 표적 항암제 개발 (Understanding centrosome amplification in cancer: A pathway toward precision-targeted cancer drug development)

  • 김태경
    • 생명과학회지
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    • 제33권11호
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    • pp.950-955
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    • 2023
  • 세포 분열은 생명체의 생존과 발달에 필수적인 과정이며, 이 과정에서 복제된 염색체가 오류 없이 정확하게 두 개로 분리되는 것이 중요하다. 중심체(centrosome)는 미세소관 형성 센터(microtubule-organizing center, MTOC)를 구성하는 핵심 기관이며, MTOC는 세포 분열과정에서 방추체를 구성하는 미세소관을 형성한다. 또한 중심체는 세포에서의 신호 경로와 운동성에 관여한다. 정상적인 세포에서 중심체는 한개씩 존재하지만, S 기에서 2개로 복제되어 세포의 양쪽 끝으로 이동하며, MTOC로부터 생성된 방추사는 복제된 염색체와 결합하여 염색체를 양쪽 끝으로 이동시킨다. 이후 세포는 두 개로 나눠져 세포 분열을 종결한다. 하지만 중심체가 정상적인 숫자보다 많은 중심체 증폭(centrosome amplification) 현상은 암세포에서 흔하게 발생하며, 이것은 염색체 불안정성(chromosomal instability, CIN)을 일으키는 원인이 될 수 있다. 본 논문에서는 중심체 복제 과정에 대해 알아보고, 이 과정에서 PLK4의 역할에 대해 알아본다. 또한 중심체 증폭이 일으킬 수 있는 결과에 대해 알아보고, 중심체 증폭의 핵심 인산화효소인 PLK4를 저해하는 약물이 어떻게 특정 종류의 암세포를 치료하는 데 있어 기여할 수 있는지 고찰해 보고자 한다.

The Fungal Metabolite Brefeldin A Inhibits Dvl2-Plk1-Dependent Primary Cilium Disassembly

  • Lee, Uijeong;Kim, Sun-Ok;Hwang, Jeong-Ah;Jang, Jae-Hyuk;Son, Sangkeun;Ryoo, In-Ja;Ahn, Jong Seog;Kim, Bo Yeon;Lee, Kyung Ho
    • Molecules and Cells
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    • 제40권6호
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    • pp.401-409
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    • 2017
  • The primary cilium is a non-motile microtubule-based organelle that protrudes from the surface of most human cells and works as a cellular antenna to accept extracellular signals. Primary cilia assemble from the basal body during the resting stage ($G_0$ phase) and simultaneously disassemble with cell cycle re-entry. Defective control of assembly or disassembly causes diverse human diseases including ciliopathy and cancer. To identify the effective compounds for studying primary cilium disassembly, we have screened 297 natural compounds and identified 18 and 17 primary cilium assembly and disassembly inhibitors, respectively. Among them, the application of KY-0120, identified as Brefeldin A, disturbed Dvl2-Plk1-mediated cilium disassembly via repression of the interaction of $CK1{\varepsilon}-Dvl2$ and the expression of Plk1 mRNA. Therefore, our study may suggest useful compounds for studying the cellular mechanism of primary cilium disassembly to prevent ciliopathy and cancer.

인공재배 상황버섯 재배종 Phellinus linteus 와 P. baumii 자실체 추출물의 항산화활성과 폴리페놀 성분 비교 (Comparison of antioxidant activities and polyphenolic compounds of extracts from artificially cultivated Sanghwang mushroom species, Phellinus linteus and P. baumii)

  • 민경진;윤봉식;강희완
    • 한국버섯학회지
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    • 제18권1호
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    • pp.29-36
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    • 2020
  • P. linteus(품종명: 고려상황, PLKS)와 P. baumii (장수상황, PBJS)를 동일한 조건하에서 인공재배하여 얻어진 자실체를 70% 메탄올, 60% 에탄올, 열수추출하여 폴리페놀과 플라보노이드 측정 시 함량은 60%에탄올 추출물에서 가장 높게 나타났다. 60%에탄올을 용매로 PLKS와 PBJS의 자실체를 추출하여 농도별로 DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbisthiazoline-6-sulfonic acid) 라디칼 소거능을 측정한 결과 PLKS와 PBJS는 거의 유사한 항산화 활성을 보였으며 자실체가 균사체보다 2-3배 이상 높은 항산화 활성이 측정되었으나 FRAP (ferric reducing antioxidant power)분석에서는 자실체와 균사체 간에 큰 차이가 없는 것을 확인하였다. PLKS와 PBJS의 자실체 ethyl acetate 분획물을 HPLC 분석한 결과 styrylpyrone 계 폴리페놀 성분인 davallialactone, hispidin, hypholomine B, inoscavin A를 검출할 수 있었으나 균사체에서는 검출되지 않아 자실체 특이적인 화합물인 것을 확인하였다.

대학수학능력시험 한국지리 과목의 위치정보 문항 출제 경향 연구: 인문(경제)지리 문항을 중심으로 (Analyzing Place Location Knowledge Items of the Korean Geography Subject in the College Scholastic Ability Test: Focusing on Human (Economic) Geography)

  • 이소영
    • 한국경제지리학회지
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    • 제24권1호
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    • pp.29-51
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    • 2021
  • 위치 지식은 지리학에서의 핵심개념이자 고차적인 지리적 사고를 위한 기초개념이다. 그럼에도 불구하고, 학생들의 위치 지식은 우리나라를 포함하여 전 세계적으로 약화되는 추세에 있다. 이러한 문제의식의 일환으로 본 연구에서는 대학수학능력시험 한국지리과목의 위치정보 문항 분석을 통해 출제의 경향성 및 학생들의 위치 지식 수준을 진단하고자 한다. 연구 결과는 다음과 같다. 첫째, 평가 요소로서의 출제 지역이 공간적으로 편중되어 나타났고, 특히 영남권에서 가장 많이 출제되었다. 둘째, 사실 기반 위치정보 문항에서는 지역특산물과 같은 지리적 표시제, 지역축제 등 지역성을 묻는 내용이 많았다. 셋째, 개념 기반 위치정보 문항의 내용은 자연지리와 인문지리영역으로 구성되는데, 경제지리와 관련된 문항은 농업 2건, 공업 2건으로 총 4문항이 출제되었다. 넷째, 학생들은 위치정보 문항을 어렵게 느끼는 경향이 있었다. 유형에 따라 어려움의 정도는 상이했는데, 학생들은 고차적 사고를 요하는 개념 기반 문항을 더 어렵게 느꼈다. 그중 경제지리 관련 문항을 두 번째로 어려워하는 것으로 나타났다. 마지막으로 출제 지역의 스케일에 따라서도 정답률의 차이가 있었는데, 학생들은 보다 정밀한 스케일에서의 문항을 상대적으로 더 어렵게 느끼는 것으로 나타났다.

Gene Expression Profiling in Osteoclast Precursors by Insulin Using Microarray Analysis

  • Kim, Hong Sung;Lee, Na Kyung
    • Molecules and Cells
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    • 제37권11호
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    • pp.827-832
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    • 2014
  • The balance between bone formation by osteoblasts and destruction of mineralized bone matrix by osteoclasts is important for bone homeostasis. The increase of osteoclast differentiation by RANKL induces bone diseases such as osteoporosis. Recent studies have shown that insulin is one of main factors mediating the cross-talk between bone remodeling and energy metabolism. However, the systemic examination of insulin-induced differential gene expression profiles in osteoclasts has not been extensively studied. Here, we investigated the global effects of insulin on osteoclast precursors at the level of gene transcription by microarray analysis. The number of genes that were up-regulated by ${\geq}1.5$ fold after insulin treatment for 6 h, 12 h, or 24 h was 76, 73, and 39; and 96, 83, and 54 genes were down-regulated, respectively. The genes were classified by 20 biological processes or 24 molecular functions and the number of genes involved in 'development processes' and 'cell proliferation and differentiation' was 25 and 18, respectively, including Inhba, Socs, Plk3, Tnfsf4, and Plk1. The microarray results of these genes were verified by real-time RT-PCR analysis. We also compared the effects of insulin and RANKL on the expression of these genes. Most genes had a very similar pattern of expressions in insulin- and RANKL-treated cells. Interestingly, Tnfsf4 and Inhba genes were affected by insulin but not by RANKL. Taken together, these results suggest a potential role for insulin in osteoclast biology, thus contributing to the understanding of the pathogenesis and development of therapeutics for numerous bone and metabolic diseases.

Properties of a Fibrinolytic Enzyme Secreted by Bacillus amyloliquefaciens RSB34, Isolated from Doenjang

  • Yao, Zhuang;Liu, Xiaoming;Shim, Jae Min;Lee, Kang Wook;Kim, Hyun-Jin;Kim, Jeong Hwan
    • Journal of Microbiology and Biotechnology
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    • 제27권1호
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    • pp.9-18
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    • 2017
  • Nine bacilli with fibrinolytic activities were isolated from doenjang, a traditional Korean fermented soy food. Among them, RSB34 showed the strongest activity and was identified as Bacillus amyloliquefaciens by 16S rRNA and recA gene sequencing. During growth on LB up to 96 h, RSB34 showed the highest fibrinolytic activity ($83.23mU/{\mu}l$) at 48 h. Three bands of 23, 27, and 42 kDa in size were observed when the culture supernatant was analyzed by SDS-PAGE and 27 and 42 kDa bands by fibrin zymography. The gene encoding the 27 kDa fibrinolytic enzyme AprE34 was cloned by PCR. BLAST analyses confirmed that the gene was a homolog to genes encoding AprE-type proteases. aprE34 was overexpressed in Escherichia coli BL21(DE3) using pET26b(+). Recombinant AprE34 was purified and examined for its properties. The $K_m$ and $V_{max}$ values of recombinant AprE34 were $0.131{\pm}0.026mM$ and $16.551{\pm}0.316{\mu}M/l/min$, respectively, when measured using an artificial substrate, N-succinyl-ala-ala-pro-phe-p-nitroanilide. aprE34 was overexpressed in B. subtilis WB600 using pHY300PLK. B. subtilis transformants harboring pHYRSB34 (pHY300PLK with aprE34) showed higher fibrinolytic activity than B. amyloliquefaciens RSB34.

PP2A function toward mitotic kinases and substrates during the cell cycle

  • Jeong, Ae Lee;Yang, Young
    • BMB Reports
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    • 제46권6호
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    • pp.289-294
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    • 2013
  • To maintain cellular homeostasis against the demands of the extracellular environment, a precise regulation of kinases and phosphatases is essential. In cell cycle regulation mechanisms, activation of the cyclin-dependent kinase (CDK1) and cyclin B complex (CDK1:cyclin B) causes a remarkable change in protein phosphorylation. Activation of CDK1:cyclin B is regulated by two auto-amplification loops-CDK1:cyclin B activates Cdc25, its own activating phosphatase, and inhibits Wee1, its own inhibiting kinase. Recent biological evidence has revealed that the inhibition of its counteracting phosphatase activity also occurs, and it is parallel to CDK1:cyclin B activation during mitosis. Phosphatase regulation of mitotic kinases and their substrates is essential to ensure that the progression of the cell cycle is ordered. Outlining how the mutual control of kinases and phosphatases governs the localization and timing of cell division will give us a new understanding about cell cycle regulation.

Mechanism, Function and Regulation of Microtubule-Dependent Microtubule Amplification in Mitosis

  • Zhu, Hui;Fang, Kayleen;Fang, Guowei
    • Molecules and Cells
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    • 제27권1호
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    • pp.1-3
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    • 2009
  • Mitotic spindle mediates the segregation of chromosomes in the cell cycle and the proper function of the spindle is crucial to the high fidelity of chromosome segregation and to the stability of the genome. Nucleation of microtubules (MTs) from centrosomes and chromatin represents two well-characterized pathways essential for the assembly of a dynamic spindle in mitosis. Recently, we identified a third MT nucleation pathway, in which existing MTs in the spindle act as a template to promote the nucleation and polymerization of MTs, thereby efficiently amplifying MTs in the spindle. We will review here our current understanding on the molecular mechanism, the physiological function and the cell-cycle regulation of MT amplification.

타구치 로버스트 계획에서 응용모형의 개발 (Development of Application Models Based on the Robust Design)

  • 최성운
    • 대한안전경영과학회지
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    • 제13권1호
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    • pp.203-209
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    • 2011
  • This study develops three new models that are practically applicable to three stages of Taguchi's robust design, which includes system design, parameter design and tolerance design. In system design, the Multiple Loss Function Analysis(MLFA) and Overall Loss Index(OLI) which reflect upon weight of characteristics and importance of specification are developed. Moreover parameter design presents Process Capability Index(PCI), $C_{PUK}$ and $C_{PLK}$, in order to segregate Signal-To-Noise Ratio(SNR) into accuracy and precision for an evaluation of relative comparison. In addition, tolerance design presents the new model of allowance computation for assembled product which is primarily derived from safety margin(SM) considering functional limit and specification. The guideline of those three new models, which include systematic charts and applicable illustrations, offers convenience for practitioners in the field. Hence, the practical applications could be made with the steps of robust designs such as system design, parameter design and specification allowance design.