• Journal of Bio-Environment Control
• /
• v.28 no.3
• /
• pp.225-233
• /
• 2019

In the summer season, natural ventilation is commonly used to reduce the inside air temperature of greenhouse when it rises above the optimal level. The greenhouse shape, vent design, and position play a critical role in the effectiveness of natural ventilation. In this study, computational fluid dynamics (CFD) was employed to investigate the effect of different roof vent designs along with side vents on the buoyancy-driven natural ventilation. The boussinesq hypothesis was used to simulate the buoyancy effect to the whole computational domain. RNG K-epsilon turbulence model was utilized, and a discrete originates (DO) radiation model was used with solar ray tracing to simulate the effect of solar radiation. The CFD model was validated using the experimentally obtained greenhouse internal temperature, and the experimental and computed results agreed well. Furthermore, this model was adopted to compare the internal greenhouse air temperature and ventilation rate for seven different roof vent designs. The results revealed that the inside-to-outside air temperature differences of the greenhouse varied from 3.2 to $9.6^{\circ}C$ depending on the different studied roof vent types. Moreover, the ventilation rate was within the range from 0.33 to $0.49min^{-1}$. Our findings show that the conical type roof ventilation has minimum inside-to-outside air temperature difference of $3.2^{\circ}C$ and a maximum ventilation rate of $0.49min^{-1}$.

• Molecules and Cells
• /
• v.42 no.6
• /
• pp.448-459
• /
• 2019

The phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway is a promising target for gastric cancer (GC) treatment; however the efficacy of PI3K/mTOR dual inhibitors in GC has not yet been maximized. Additionally, the effect of autophagy regulation by PI3K/mTOR dual inhibitors has not been clearly elucidated in GC treatment. We aimed to show that our newly developed PI3K/mTOR dual inhibitor, CMG002, when combined with an autophagy inhibitor, chloroquine (CQ), potently induces effective cancer cell death in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) cells, where both the PI3K/AKT/mTOR and autophagy pathways play important roles in disease pathogenesis. EBV- and mock-infected AGS and NUGC3 GC cell lines were treated with CMG002 +/- CQ. PI3K/AKT/mTOR signaling pathway mediators, cellular apoptosis and autophagy markers were confirmed by Western blot assay. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay. CMG002 effectively blocked the PI3K/AKT/mTOR pathway by markedly decreasing phosphorylation of AKT and its downstream mediator S6. CMG002 induced G0/G1 cell cycle arrest and enhanced apoptotic cell death in AGS and NUGC3 cells, particularly EBV-infected cells compared with mock-infected cells, as confirmed by flow cytometric analyses and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assays. The combination of CMG002 plus CQ synergistically increased apoptotic cell death in EBV-infected GC cell lines when compared with CMG002 alone (P < 0.05). Our results suggest that the new PI3K/mTOR dual inhibitor, CMG002, when used in combination with the autophagy inhibitor, CQ, provides enhanced therapeutic efficacy against EBVaGC.

• Asian-Australasian Journal of Animal Sciences
• /
• v.32 no.5
• /
• pp.614-628
• /
• 2019

Objective: The inhibitory leukocyte immunoglobulin-like receptors (LILRBs) play an important role in innate immunity. The present study represents the first description of the cloning and structural and functional analysis of LILRB1 and LILRB3 isolated from two genetically disparate chicken lines. Methods: Chicken LILRB1-3 genes were identified by bioinformatics approach. Expression studies were performed by transfection, quantitative polymerase chain reaction. Signal transduction was analyzed by western blots, immunoprecipitation and flow cytometric. Cytokine levels were determined by enzyme-linked immunosorbent assay. Results: Amino acid homology and phylogenetic analyses showed that the homologies of LILRB1 and LILRB3 in the chicken line 6.3 to those proteins in the chicken line 7.2 ranged between 97%-99%, while homologies between chicken and mammal proteins ranged between 13%-19%, and 13%-69%, respectively. Our findings indicate that LILRB1 and LILRB3 subdivided into two groups based on the immunoreceptor tyrosine-based inhibitory motifs (ITIM) present in the transmembrane domain. Chicken line 6.3 has two ITIM motifs of the sequence LxYxxL and SxYxxV while line 7.2 has two ITIM motifs of the sequences LxYxxL and LxYxxV. These motifs bind to SHP-2 (protein tyrosine phosphatase, non-receptor type 11) that plays a regulatory role in immune functions. Moreover, our data indicate that LILRB1 and LILRB3 associated with and activated major histocompatibility complex (MHC) class I and ${\beta}2-microglobulin$ and induced the expression of transporters associated with antigen processing, which are essential for MHC class I antigen presentation. This suggests that LILRB1 and LILRB3 are transcriptional regulators, modulating the expression of components in the MHC class I pathway and thereby regulating immune responses. Furthermore, LILRB1 and LILRB3 activated Janus kinase2/tyrosine kinase 2 (JAK2/TYK2); signal transducer and activator of transcription1/3 (STAT1/3), and suppressor of cytokine signaling 1 genes expressed in Macrophage (HD11) cells, which induced Th1, Th2, and Th17 cytokines. Conclusion: These data indicate that LILRB1 and LILRB3 are innate immune receptors associated with SHP-2, MHC class I, ${\beta}2-microglobulin$, and they activate the Janus kinase/signal transducer and activator of transcription signaling pathway. Thus, our study provides novel insights into the regulation of immunity and immunopathology.

• The Korea Journal of Herbology
• /
• v.36 no.3
• /
• pp.47-53
• /
• 2021

Objectives : Currently, the alopecia is one of the most emotionally stressful syndromes in human life. Human hair dermal papilla cells (HDPCs) play an essential role in controlling hair growth and in regulating hair cycle. We performed MTT assay, cell cycle, and western blot to determine the effects of essential oil from Coicis Semen (ECS) on hair growth in HDPCs. Methods : We monitored cell proliferations by MTT assay in HDPCs. After setting up the safe and effective concentration range to be treated ECS, cell cycle analysis was performed using flow cytometry. Also, the protein expression of hair growth-related factors such as insulin like growth factor-1 (IGF-1), Wnt, extracellular signal-regulated kinase (ERK), serine/threonine-specific protein kinase (Akt) in HDPCs was determined by western blot. Results : As results, cell proliferation was increased in ECS group compared to dimethyl sulfoxide (DMSO) group and minoxidil (MNXD) group. Cell number of ECS group was more decrease in sub G1 phase than cell number of DMSO group. Also, cell number of ECS group increased compared to cell number of DMSO group in G1 phase. Protein expression of ECS group was higher than protein expression of DMSO group on related hair growth factors (IGF-1, Wnt, ERK, Akt). Conclusion : As mentioned above, ECS increased cell proliferation and the protein expression of IGF-1, Wnt, ERK, and Akt. These results suggest that ECS could be used as a potential material for the treatment of alopecia by increasing the proliferation of HDPCs.

• Journal of Marine Life Science
• /
• v.4 no.2
• /
• pp.53-62
• /
• 2019

Heterotrophic bacteria are a major member of the microbial loop in the marine ecosystem and play an important role in the biogeochemical cycle decomposing organic matter. Therefore study of bacterial variation is important to understand the material cycle and energy flow of marine ecosystems. We investigated the monthly variations of bacteria and environmental factors in the Youngsan River estuary, and the correlation between bacteria and phytoplankton biomass (chlorophyll-a) including size-structure. As a result, bacteria of the Youngsan River estuary were higher in the surface than in the bottom layer, and higher in the summer than in winter. And the closer to the dike, the abundance increased, and it increased to the peaks in August, September, and June 2019 at the station closest to the dike. The chlorophyll-a also increases at the stations and time when the bacterial abundance was high and they correlates positively displaying no difference between size fractions. The results indicate that organic matter derived from phytoplankton has an effect on bacterial variation but no size-dependent effects. In addition, the seasonal pattern of bacteria increasing in proportion to the water temperature suggests the effect of water temperature on the growth of bacteria. No association of bacterial abundance variation with nutrient supply due to freshwater input was observed. In this study, dissolved oxygen was depleted and hypoxia was observed for a short time when a strong stratification was not developed. This may be resulted from the supply of organic matter from phytoplankton and the consumption of oxygen due to bacterial decomposition.

• Journal of the Korea Society of Computer and Information
• /
• v.27 no.5
• /
• pp.199-204
• /
• 2022

The purpose of this study is to broaden the understanding of the Security Services Industry Act, and also to examine the meanings of various phenomena by analyzing the media report big data rather than the researchers' perspective on the Security Services Industry Act. In the research method, this study searched for a keyword 「Security Services Industry Act」 that prescribes the security work as an important subject of crime prevention and maintenance of public order in Korea. The data was searched from 1990 to 2021 the BIG KINDS could provide. Also, for the concrete analysis during the period of data search, it was divided into settlement period(1976~2001), growth period-quantitative(2002~2012), and growth period-qualitative(2013~2021). In the results of this study, the media report perception of the Security Services Industry Act is continuously emphasizing the social roles and importance of private security according to the flow of time. The consequent marketability of private security will play great roles in the protection of people's lives and properties in the combination with various other industries in the future. However, the private security industry that provides public peace service together with the police, could be rising as an element that hinders the development of private security industry because of various social issues caused by legal regulations and illegal problems, so it would be necessary to more strengthen its responsibility and roles accordingly.

• Journal of Life Science
• /
• v.33 no.5
• /
• pp.397-405
• /
• 2023

Although glutathione (GSH) has been shown to play an important role in the prevention of oxidative damage as an antioxidant, studies on immune regulation by it have not been properly conducted. In this study, we investigated whether luthione^®, a reduced GSH, has an immune enhancing effect in murine macrophage RAW 264.7 cells. The results of flow cytometry and immunofluorescence experiments indicated that luthione increased phagocytic activity, a representative function of macrophages, compared to the control cells. According to the results of the cytokine array, the expression of interleukin (IL)-5, IL-1β, and IL-27 was significantly increased in the luthione-treated cells. Luthione also enhanced the production of tumor necrosis factor-α and IL-1β through increased expression of their proteins, and increased release of the immune mediators such as nitric oxide (NO) and prostaglandin E₂ was associated with increased expression of inducible NO synthase and cyclooxygenase-2. In addition, the expression of cluster of differentiation 86, an M1 macrophage marker, was dramatically enhanced in RAW 264.7 cells treated with luthione. Furthermore, as a result of heat map analysis, we found that cytokine signaling 1/3-mediated signal transducer and activator of transcription/Janus tyrosine kinase signaling pathway was involved in the immunomodulatory effect by luthione. In conclusion, our data suggested that luthione could act as a molecular regulator in M1 macrophage polarization and enhance immune capacity by promoting macrophage phagocytic function.

• Tunnel and Underground Space
• /
• v.33 no.4
• /
• pp.228-243
• /
• 2023

Bentonite is widely recognized and utilized as a buffer material in high-level radioactive waste repositories, mainly due to its favorable characteristics such as swelling capability and low permeability. Bentonite buffers play an important role in ensuring the safe disposal of radioactive waste by providing a low permeability barrier and effectively preventing the migration of radionuclides into the surrounding rock. However, the long-term performance of bentonite buffers still remains a subject of ongoing research, and one of the main concerns is the erosion of the buffer induced by swelling and groundwater flow. The erosion of the bentonite buffer can significantly impact repository safety by compromising the integrity of buffer and leading to the formation of colloids that may facilitate the transport of radionuclides through groundwater, consequently elevating the risk of radionuclide migration. Therefore, it is very important to numerically quantify the erosion of bentonite buffer to evaluate the long-term performance of bentonite buffer, which is crucial for the safety assessment of high-level radioactive waste disposal. In this technical note, Two-region model is introduced, a proposed model to simulate the erosion behavior of bentonite based on a dynamic bentonite diffusion model, and quantitative evaluation is conducted for the bentonite buffer erosion with this model.

• Journal of Korea Entertainment Industry Association
• /
• v.13 no.2
• /
• pp.101-110
• /
• 2019

Filmic spaces are not only a core expressive factors that can embody both internal and external meanings of films, but also play an important role for initiating dramatic context in terms of narratives. They establish visible environment in which provocative incidents occur and characters' behaviors are induced, include characters' psychology or emotional meanings of narratives and provoke tensions through symbolic meanings and the implicit function of surface background. This paper, therefore, analyzes the film, , by focusing on the dramatic function of narrative space. This film provides insights and thoughts through deep philosophical reflection, by escaping from the convention of such a genre, through deep philosophical reflection, by escaping from the convention of such a genre, though it belongs seemingly to the crime thriller genre using Mexico's drug cartel as it main material. In the film, narrative spaces are responsible for emotions invoking dramatic tensions beyond provocative incidents and the elaborately planned and controlled mise-en-scene absorbed audiences' attention by organizing ultimate suspense. In conclusion, flow and dramatic lingering imagery of this film might be achieved by power of scenes rather than plot factors. This study thus explained the correlation between narrative spaces and dramatic immersion, by analyzing spaces appearing in and visualized construction. It is hoped that this will further extends the pattern of researches on dramatic immersion, which have been primarily focused on plots and characters.

• Journal of Korea Water Resources Association
• /
• v.55 no.spc1
• /
• pp.1283-1293
• /
• 2022

The purpose of this study is to predict residual chlorine in order to maintain stable residual chlorine concentration in sedimentation basin by using artificial intelligence algorithms in water treatment process employing pre-chlorination. Available water quantity and quality data are collected and analyzed statistically to apply into mathematical multiple regression and artificial intelligence models including multi-layer perceptron neural network, random forest, long short term memory (LSTM) algorithms. Water temperature, turbidity, pH, conductivity, flow rate, alkalinity and pre-chlorination dosage data are used as the input parameters to develop prediction models. As results, it is presented that the random forest algorithm shows the most moderate prediction result among four cases, which are long short term memory, multi-layer perceptron, multiple regression including random forest. Especially, it is result that the multiple regression model can not represent the residual chlorine with the input parameters which varies independently with seasonal change, numerical scale and dimension difference between quantity and quality. For this reason, random forest model is more appropriate for predict water qualities than other algorithms, which is classified into decision tree type algorithm. Also, it is expected that real time prediction by artificial intelligence models can play role of the stable operation of residual chlorine in water treatment plant including pre-chlorination process.