• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.352-358
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• 1985

This study was carried out to identify the protein patterns and to estimate molecular weights of the ginseng and other plants. Polyacrylamide gradient 2-30% gels for identification of protein patterns and 11% SDS polyacrylamide gels for molecular weight estimation of various plants were used. Proteins extracted with distilled water and phosphate buffer were most clearly separated. Although minor bands were a little different according to kinds of buffer, major bands showed similar pattern. Major protein bands were two bands of 45,000, 66,000 in Panax ginseng, 3 bands of 32,000-39,000 in Codonopsis lanceolata and one double band around 39,000 kd in Platycodon grandiflorum.

• YAKHAK HOEJI
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• v.59 no.4
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• pp.170-176
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• 2015

In vaccine development, the major points may be induction of effective and increased levels of antibody production. This is especially the case when the antigenic sources are carbohydrates. For many years, thus, we have researched various types of formulations such as liposomal and conjugate vaccines. However, the fastidious formulation process and high costs are a problem. For this reason, there is currently a focus on utilizing immunoadjuvants. In this present study, we tested if platycodin D (PLD) from Platycodon Radix have immunoadjuvant activity against the cell wall of Candida albicans (CACW). The resulting data showed that in the murine model of antibody production, CACW combined with PLD [CACW/PLD/IFA] increased the production of antibodies specific to C. albicans when compared to the antibody production by [CACW/IFA]-induction, which was used as a negative control (P<0.05). In the case of [CACW/PLD/IFA], the antibody production was 1.4 times as that of the CFA. In addition, formulations containing either had a prolonged antibody inducing activity maintaining the initial titers of antibody as compared to the CFA formula. Cytokine profiling with the antisera displayed that the PLD produced both Th1 and Th2 immunoresponses, but Th1 dominant was much greater (P<0.05). Furthermore, [CACW/PLD/IFA] formula enhanced resistance of mice against disseminated candidiasis, whereas the CFA had no such effect. In conclusion, PLD has an immunologic activity, which is protective against the disease. Thus, PLD can be a goof candidate for a new immunoadjuvant in development of the fungal vaccine.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.219-226
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• 1994

Immature ovule of intergeneric $F_1$ hybrid between Platycodon grandiflorum x Codonopsis lanceolata for producing embryogenic callus. somatic embryos and plant regeneration were cultured in vitro on various medium as well as MT(Murashige Tucker)medium treated with different concentration of plant growth regulators. Embryogenic callus induction was highest in the treatment of NAA 0.5 $mg/{\ell}$ and zeatin 0.01 $mg/{\ell}$ added on MT medium, whereas it was lower in treatments with auxins alone. MT medium were more effective in production of somatic embryos from incubated embryogenic callus. Most favorable plant growgh regulator for producing somatic embryos was 2. 4-D 0.5 $mg/{\ell}$ and zeation. BAP 0.01$mg/{\ell}$, but hormone-free and auxins alone were less effective. NAA 0.01$mg/{\ell}$ added with zeation 0.5 $mg/{\ell}$ was effective as high as NAA 0.01 $mg/{\ell}$ alone in normal plant regeneration from somatic embryo.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.437-441
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• 2004

These studies were conducted to investigate the proper method of nitrogen application for increasing the growth stability and quality in ballon flower (Platycodon grandiflorum A. DC.). In growth characteristics by split ratio of nitrogen application, stem diameter, number of branching roots and number of leaves were higher at the split ratio of 50:50 (nitrogen were splitted a half with basal application and the other half in autumn) than at 70:30. Root length, root diameter and number of branching root were higher at 50:50 than those of 70:30. The root yield was increased by 30% (230 kg/10a) at 50:50 compared with that of the 70:30. The crude saponin contents of root from 50:50 was 4.68%.

• Korean Journal of Medicinal Crop Science
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• v.7 no.4
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• pp.282-287
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• 1999

This study was conducted to investigate the effects of different soil texture in agronomic characteristics, root yield and quality of baloon flower (Platycodon grandiflorum A. DC.). Three kinds of soil texture, sandy loam, silt loam and clay loam, were tested for two years. Baloon flowers were seeded with 20cm row space and cultivated in the one square meter pot with 0. 6m depth. Fifty plants per pot were remained. In growth characteristics of one-year-old plants, shooting date was not significantly different according to the changes of soil texture. The ratio of stem growth was highest between July and August. In two-years-old plants, it was showed between May and June. Plants grown on clay loam showed the highest growing characteristics than those of other soil textures. Dry root weight cultivated on clay loam was 274kg/10a in one-year-old and 934kg/10a in two-years-old plants. The content of ethanol extracts from root was highest at those of sandy loam as 47.1 % in one-year-old root. The crude saponin content was not different significantly among the three kinds of soil texture.

• Journal of Life Science
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• v.23 no.3
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• pp.389-398
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• 2013

Platycodin D is a major constituent of triterpene saponins, which is found in the root of Platycodon grandiflorum, Platycodi Radix, which is widely used in traditional Oriental medicine for the treatment of many chronic inflammatory diseases. Several pharmacological effects of this compound have been reported recently, such as anti-inflammation, immunogenicity, anti-adipogenesis, lowered cholesterol, and anti-cancer activity. However, the mechanism by which this action occurs is poorly understood. In this study, we found that platycodin D greatly increased the potential of the anti-proliferative effect in various cancer cell lines. Our data revealed that platycodin D treatment resulted in a time- and concentration-response growth inhibition of U937 cells by inducing apoptosis, as evidenced by the formation of apoptotic bodies, chromatin condensation, and the accumulation of cells in the sub-G1 phase. Apoptosis induction of U937 cells by platycodin D correlated with an increase in the Bax/Bcl-2 ratio and caused the down-regulation of IAP family members. In addition, platycodin D treatment resulted in proteolytic activation of caspase-3, the concomitant degradation of poly(ADP-ribose) polymerases, and the collapse of the mitochondria membrane potential (${\Delta}{\Psi}_m$). However, the cytotoxic effects induced by platycodin D treatment were significantly inhibited by z-DEVD-fmk, a caspase-3 inhibitor, which demonstrated the important role that caspase-3 played in the observed cytotoxic effect. These findings suggest that platycodin D may be a potential chemotherapeutic agent for use in the control of human leukemia U937 cells. These findings also provided important new insights into possible molecular mechanisms of the anti-cancer activity of platycodin D.

• Korean Journal of Medicinal Crop Science
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• v.5 no.2
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• pp.139-146
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• 1997

Priming has been used to establish a better standing in practice as controling the seed moisture content after sowing. The experiment was done to measure the effect of priming (material ; concentration ; period) and light quality (red ; white ; dark) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum ; Codonopsis lanceolata ; C. pilosula) to give an information on their earlier standing establishment. The germination test was carried out with 12 hours irradiation for 9 days after priming treatment. In the darkness, the mean germination rate of all the species was decreased in the order to P. grandiflorum, C. pilosula, C. lanceolata. Their germination and radicle elongation became more inclined when primed with $Ca(CO_3)_2$ or with 50 to 150mM than with $KNO_3$ or no priming although there was no difference between priming periods. Under irradiation during their seed germination, however, the former order was changed to P. grandiflorum, C. lanceolata, C. pilosula because light quality treatment given after priming reduced the rate of C. pilosula but enhanced that of C. lanceolata. Although light quality forced after priming did not affect the mean germination rate of P. grandiflorum, it increased earlier or alltime germination of C. lanceolata or C. pilosula, respectively. White light after priming repressed germinations of C. lanceolata and C. pilosulai but increased earlier germinatin of P. grandiflorum, meaning that seed germination and radicle elongation of Campanulaceae could be determined by light quality treated after priming.

• 한국약용작물학회:학술대회논문집
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• 2007.05a
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• pp.241-242
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• 2007

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.267-268
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• 2008

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.263-264
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• 2008