• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2002.11a
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• pp.184-184
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• 2002

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.206.2-207
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• 2003

The immunostimulatory and host resistance effects of the Platycodon grandiflorum A. DC, changkil (CK) and inulin (CKI) isolated from CK were investigated in rats. SD rat were exposed to CK or CKI by gavages for 7days and isolated peritoneal macrophages and splenocyte were used for these studies. CK and CKI significantly enhanced peritoneal macrophages activities such as ROS production and phargocytosis. (omitted)

• Korean Journal of Pharmacognosy
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• v.21 no.4
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• pp.290-299
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• 1990

Taxonomic relationships between some inulin containing plants such as Platycodon and Codonopsis have been investigated comparing their contents of inulin crystals, resin, as well as TLC patterns of saponins and acetylenes. Although Platycodon and Codonopsis have been known to be close in anatomic characters, the present results showed that there were significant differences between not only in the genus but also in the species.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.11b
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• pp.184-184
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• 2002

In our previous study, we reported that PG, a polysaccharide isolated from Plyatycodon grandiflorum, activated macrophages and B cells, but not T cells. Here, we investigated in more detail the mechanism of action of PG in macrophage activation.(omitted)

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.176.1-176.1
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• 2003

The protective effects on hepatic fibrosis of an aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil (CK), in hepatic stellate cell line, CFSC-2G. The increased deposition of extracellular matrix by hepatic stellate cells following liver injury in a process known as activation is considered a key mechanism for increased collagen content of liver during the development of liver fibrosis. (omitted)

• 공원문화
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• s.64
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• pp.16-17
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• 1996

• Korean Journal of Plant Resources
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• v.26 no.3
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• pp.389-396
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• 2013

The antioxidant enzyme and DPPH radical scavenging activity with variations in drying methods of diploid and tetraploid in Platycodon grandiflorum were determined. Antioxidant enzyme activities were measured as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), and ascorbate peroxidase (APX). The roots of Platycodon grandiflorum were freeze-dried, indoor-dried, hot-air dried, and microwave dried. The root extract of P. grandiflorum have shown the highest SOD enzyme activity of 92% in tetraploid of freeze-dried and indoor-dried while diploid of microwave dried showed the lowest SOD enzyme activity of 47.5%. The activity of CAT showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all drying methods. The APX activity showed relatively higher values in the root extract of freeze-dried both the diploid and tetraploid, but the difference in comparison with other extracts was not significant. The POX activities according to drying methods of diploid and tetraploid in P. grandiflorum showed relatively high values in freeze-dried and indoor-dried compared with other drying methods, and the POX activity between the diploid and tetraploid was not significant difference in each drying method. The DPPH radical scavenging activity with variation in drying methods of diploid and tetraploid in P. grandiflorum was the highest in the freeze-dried, and was higher in tetraploid than diploid in all the concentrations. In conclusion, the root of P. grandiflorum had the potent biological activities in both diploid and tetraploid. In particular, the tetraploid root of P. grandiflorum showing high antioxidant enzyme activity could be good materials for development of source of functional healthy food.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.172-176
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• 1999

The saponins are considered the main effective components in Platycodon grandiflorum (Jacq.) A. DC.. In order to obtain the basic information for producing the high quality medicinal plant and processing, the crude saponin contents were analyzed with platycodi radix, the root of Platycodon grandiflorum by different cultivating years, parts, harvesting times and drying methods. The crude saponin contents were decreased by increasing cultivating years. The crude saponin contents were 2.74% in tail of root and 1.65 % in head of root, respectively. Besides, the contents of the crude saponin in cortex were 1.8 times more than that in core of root. The crude saponin contents in different harvesting times were 2.82% and 2.74% at March 10 and December 10, showing higher than that being harvested at June 10 and September 10. The crude saponin contents among drying methods were not significantly different at 5% level DMRT, but the hot air drying after steam showed the least crude saponin.

• Environmental Analysis Health and Toxicology
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• v.25 no.1
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• pp.85-94
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• 2010

Platycodon grandiflorum, Doraji as Korean name, is one of the most widely used traditional oriental medicine for bronchial diseases and also used as a folk remedy for geriatric diseases and inflammatory diseases. In recent studies, it has been reported that some effect of P. grandiflorum is derived from its antioxidant activity, although there is still a lack of evidence to establish its oxy-radical scavenging activity. In this study, total oxy-radical scavenging capacity (TOSC) assay was used to evaluate antioxidant activity of total extracts (T-PG), polysaccharide fraction (Po-PG), and saponin fraction (Sa-PG) isolated from P. grandiflorum against peroxyl radicals and peroxynitrites. And MTT assay was taken to assess cyto-protective effects of T-PG, Po-PG and Sa-PG in H4IIE cells treated with hydrogen peroxide and tert-butylhydroperoxide. In the TOSC assay, Sa-PG showed strong oxy-radical scavenging capacity compared with T-PG and Po-PG. In cell-based assay, T-PG and Po-PG protected cells from oxidative stress, but Sa-PG did not protect cells because of cytotoxicity of Sa-PG. These results suggest that the saponin components of P. grandiflorum have relatively strong antioxidant capacity and cytotoxicity in rat hepatoma cells.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.123-123
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• 2017

In spite of the potential medicinal significance and a wide range of pharmacologic properties of Platycodon grandiflorum, the molecular mechanism of its roots is still unknown. The present study was conducted to profile proteins from 3, 4 and 5 months aged diploid and tetraploid roots of Platycodon grandiflorum using high throughput proteome approach. Two-dimensional gels stained with CBB, a total of 68 differential expressed proteins were identified from the diploid root out of 767 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 29 differential expressed protein spots (${\geq}2-fold$) were analyzed using LTQ-FTICR MS whereas a total of 24 protein spots were up-regulated and 5 protein spots were down-regulated. On the contrary, in the case of tetraploid root, a total of 86 differential expressed proteins were identified from tetraploid root out of 1033 protein spots of which a total of 39 differential expressed protein spots (${\geq}2-fold$) were analyzed using LTQ-FTICR MS whereas a total of 21 protein spots were up-regulated and a total of 18 protein spots were down-regulated. It was revealed that the identified proteins from the explants were mainly associated with the nucleotide binding, oxidoreductase activity, transferase activity. Taken together, the identified proteins may be helpful to identify key candidate proteins for genetic improvement of plants.