• Journal of Periodontal and Implant Science
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• 제31권3호
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• pp.513-529
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• 2001

For reconstruction of the bony defect, various artificial substitutes were developed. Among them, there has been a study of calcium phosphate coated bone substitutes for increasing attachment of osteoblasts in vivo. The purpose of this study was to evaluate the effects of serum and platelet-rich plasma (PRP) on calcium phosphate coated culture plate for the initial attachment, proliferation and activity of osteoblasts. After sampling the blood from white rats and concentrating by centrifugation, the amount of attachment of PDGF-BB and $TGF-{\beta}$ on the calcium phosphate coated culture plate was measured. Cultured HOS and ROS 17/2.8 cell was measured on attachment level and proliferation rate of osteoblasts. Alkaline phosphatase activity of HOS and ROS 17/2.8 cell was measured for studying on the activating rate of osteoblast. 1. Counting the amount of platelets of seperated plasma and PRP, the average number of platelets was 177,003 $cell/{\mu}l$ in plasma, and 1,656,062 $cell/{\mu}l$ in PRP, which was about 9 times as high as in plasma. 2. Amount of PDGF-BB deposited at calcium phosphate coated plate had increased by the total amount of plasma and PRP on the culture plate, whereas $TGF-{\beta}$had been deposited on the plate only when treated by $50{\mu}{\ell}$ of PRP(p<0.01). 3. After plating serum and PRP for 3 hours, we attached with HOS and ROS17/2.8 cell for 1 hour and 4 hours. There were no significant difference of the attachment between serum and control group, whereas there were significantly difference of the attachment between depositioning of PRP and control group. 4. After attaching plasma and PRP for 3 hours, cell number has much increased when HOS and ROS17/2.8 cell had been cultured for 48 hours(p<0.05). 5. After attaching plasma and PRP for 3 hours, concentration of alkaline-phosphatase has increased when HOS and ROS17/2.8 cell had been cultured for 48 hours(p<0.01). These results suggested that PRP affected on initial cell attachment rather than proliferation and activation of osteoblasts at calcium phosphate coated plate.

• Restorative Dentistry and Endodontics
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• 제44권4호
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• pp.35.1-35.10
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• 2019

Pulp revascularization is an alternative treatment in immature traumatized teeth with necrotic pulp. However, this procedure has not been reported in horizontal root fractures. This is a case report of a 9-year-old patient with multiple horizontal root fractures in 2 upper central incisors that were successfully treated with pulp revascularization. The patient presented for treatment 2 years after the initial trauma, and revascularization was attempted after the initial treatment with calcium hydroxide had failed. Prior to pulp revascularization, cone-beam computed tomography and autoradiograms demonstrated multiple horizontal fractures in the middle and apical thirds of the roots of the 2 affected teeth. Revascularization was performed in both teeth; platelet-rich plasma (PRP) was used in one tooth (#11) and the conventional method (blood clot) was used in the other tooth (#21). Clinical and radiographic follow-up over 4 years demonstrated pulp calcification in the PRP-treated tooth. Neither of the 2 teeth were lost, and the root canal calcification of tooth #11 was greater than that of tooth #21. This case suggests that PRP-based pulp revascularization may be an alternative for horizontal root fractures.

• Journal of Korean Dental Science
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• 제12권1호
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• pp.5-12
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• 2019

Purpose: To determine the benefits of autogenous tooth bone (ATB) graft in combination with platelet-rich plasma (PRP) in the rates of success and survival of dental implants placed simultaneously with maxillary sinus floor augmentation (MSFA). Materials and Methods: Patients who visited the Department of Oral and Maxillofacial Surgery at Ulsan University Hospital from 2012 to 2014 and underwent simultaneous placement of implants with MSFA using ATB plus PRP were included in the study. Success and survival rates of the implants were evaluated based on the parameters of age and sex of the patient, site, follow-up period, residual bone height before surgery, diameter, and length of implant, sinus mucosa impairment, and postoperative complications. Result: A total of 23 patients and 67 implants were included in this study. The average age of the patients was $53.78{\pm}10.00$ years. The average follow-up period after installation of the prosthesis was $53{\pm}5$ months. The success and survival rates of the implants after placement of prosthesis were 95.52% and 97.01%, respectively. Conclusion: Combination of ATB and PRP showed high overall success rate, and it can be concluded that this combination is a predictable bone graft procedure for MSFA.

• 한국임상수의학회지
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• 제40권2호
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• pp.85-92
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• 2023

Fractures in the horse industry are challenging and a common cause of death in racehorses. To accelerate fracture healing, tissue engineering (TE) provides promising ways to regenerate bone tissues. This study aimed to evaluate the osteogenic effects of biphasic calcium phosphate collagen (BCPC) graft, bone morphogenetic protein 2 (BMP2), mesenchymal stem cell (MSC), and platelet-rich plasma (PRP) treatments in horses. Four thoroughbred horses were included in the study, and, in each horse, three cortical defects with a diameter of 5 mm and depth of 10 mm were formed in the third metacarpal bones (MC) and metatarsal bones (MT). The defects were randomly assigned to one of six treatment groups (saline, BCPC, BMP2, MSC, PRP, and control). Injections of saline, BMP2, PRP, or MSCs were made at 1, 3, and 5 weeks after defect surgery. Bone regeneration effects were assessed by radiography, quantitative computed tomography (QCT), micro-computed tomography (μCT), histopathological, and histomorphometric evaluation. The new bone ratio (%) in the histomorphometric evaluation was higher in the BMP2 group than in the control and saline groups. Radiographic and QCT values were significantly higher in the BCPC groups than in the other groups. QCT values of the BMP2 group were significantly higher than in the control and saline groups. The present study demonstrated that BCPC grafts were biologically safe and showed osteoconductivity in horses and the repeated injections of BMP2 without a carrier can be simple and promising TE factors for treating horses with bone fractures.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제38권
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• pp.16.1-16.9
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• 2016

Background: The association of biomaterial combined with repair factor-like platelet-rich plasma (PRP) has prospective values. Bovine-derived xenograft has been identified as an osteoconductive and biocompatible grafting material that provides osseointegration ability. PRP has become a valuable adjunctive agent to promote healing in a lot of dental and oral surgery procedures. However, there are controversies with respect to the regenerative capacity of PRP and the real benefits of its use in bone grafts. The purpose of this study was to assess the influence of PRP combined with xenograft for the repair of peri-implant bone defects. Methods: Twelve rabbits were used in this study, and the experimental surgery with implant installation was performed simultaneously. Autologous PRP was prepared before the surgical procedure. An intrabony defect (7.0 mm in diameter and 3.0 mm deep) was created in the tibia of each rabbit; then, 24 titanium dental implants (3.0 mm in diameter and 8.5 mm long) were inserted into these osteotomy sites. Thus, a standardized gap (4.0 mm) was established between the surrounding bony walls and the implant surface. The gaps were treated with either xenograft alone (control group) or xenograft combined with PRP (experimental group). After healing for 1, 2, 3, 4, 5, and 6 weeks, the rabbits were sacrificed with an overdose of KCl solution. Two rabbits were killed at each time, and the samples including dental implants and surrounding bone were collected and processed for histological analysis. Results: More newly formed bone and a better bone healing process were observed in control group. The histomorphometric analysis revealed that the mean percentage of bone-to-implant contact in the control group was significantly higher than that of the experimental group (25.23 vs. 8.16 %; P < 0.05, independent-simple t test, analysis of variance [ANOVA]). Conclusions: The results indicate that in the addition of PRP to bovine-derived xenograft in the repair of bone defects around the implant, PRP may delay peri-implant bone healing.

• Journal of Periodontal and Implant Science
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• 제32권2호
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• pp.325-338
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• 2002

Bone graft using growth factors and guided tissue regeneration have been used for the regeneration of infrabony defects which caused by periodontal disease. Calcium sulfate which is one of the resorbable barrier materials used for guided tissue regeneration. Platelet rich plasma which is a easy method to obtain the growth factors had many common points but, platelet rich plasma was still studying. This study was the comparative study between bone graft using platelet rich plasma and guided tissue regeneration using calcium sulfate barrier material in clinical view. For the study, 28 sites(2 or 3 wall infrabony defects) were treated. 14 infrabony defects were received surgical implantation of BBP-calcium sulfate composite with a calcium sulfate barrier and the others received BBP mixed with platelet rich plasma. Clinical outcome was accessed 3 and 6 months of postsurgery. 1. There was no statistical difference between CS group and PRP group in pocket depth, gingival recession, clinical attachment level, and probing bone level at baseline. 2. There was statistically significant reduction in probing depth, clinical attachment level, and probing bone level at 3 and 6 months postsurgery(p<0.05). 3. In the probing depth and clincial attachment level PPR group had less improvement than CS group, but there was no statistically difference at 3 and 6 months postsurgery. 4. In the recession PPR group had less recession than CS group, but there was no statistically difference at 3 and 6 months postsurgery. 5. In the probing bone level PPR group had less improvement than CS group, but there was no statistically difference at 6 months postsurgery. In conclusion bone graft using platelet rich plasma and guided tissue regeneration using calcium sulfate barrier showed similar clinical improvement for the treatment of 2 or 3 wall infrabony defects.

• 한국임상수의학회지
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• 제40권2호
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• pp.93-103
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• 2023

The tendon is a dense connective tissue that connects muscle to bone and plays an essential role in joint motion. The injured tendon heals slowly owing to its low cellularity and vascularity. This study aimed to evaluate and compare the effects of regenerative injection therapy (RIT), 20 % dextrose prolotherapy (DP), and platelet-rich plasma (PRP) injections that can promote tendon healing. Twenty-one New Zealand white rabbits were divided into the control, DP, and PRP treatment groups. The superficial digital flexor tendon (SDFT) of the right hindlimb of each rabbit was used. A round defect of 2 mm was induced. Approximately 0.2 mL of 20% dextrose and autologous PRP were injected into the proximal and distal ends of the SDFT mass. Radiographic and ultrasonographic examination and cross-sectional area (CSA) calculations were performed pre-operatively and at 2, 4, and 8 weeks. The SDFT of both limbs was transected for biomechanical and histomorphometric evaluations. The SDFT of the left limb was transected for intact control. Semi-quantitative analysis was performed to evaluate the histomorphometric properties. Additional analysis was performed using H&E, Masson's trichrome, and immunohistochemical staining. The biomechanical evaluation showed that the treatment groups had higher tensile strength compared to the defect control group, while the PRP group had higher tensile strength than the DP group. On histological examination, the treatment groups appeared to be relatively closer to the remodeling phase of the healing process than the defect control group; the characteristics of the PRP group were closer to the remodeling phase than those of the DP group. The ultrasonographic examination showed different tendencies. Increased values in the CSA were observed during the early period in the treatment groups. This study suggests that PRP and DP can promote the healing of tendon injury, and these effects were superior with PRP than that with DP.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.280-280
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• 1994

혈행 장애 예방 및 치료 약물의 개발을 목적으로 혈소판 기능에 대한 약물의 영향을 연구하는데 있어, 효율적인 실험 조건의 선택은 중요하다. 본 연구에서는 마늘 메탄을 추출물의 혈소판 응집 억제능율 platelet rich plasma(PRP), washed platelet(Ca$^2$비첨가), washed platelet(Ca$^{2+}$ 첨가) 등 세가지 분리 조건을 사용하여 실험하였다. 그 결과 마늘 추출물의 $IC_{50}$/이 각각 2348 $\mu\textrm{g}$/ml, 221 $\mu\textrm{g}$/ml, 851 $\mu\textrm{g}$/ml로서, washed platelet(Ca$^{2+}$ 비첨가)가 마늘 추출물의 혈소판 응집 억제 작용에 가장 민감한 것으로 나타났다. 또한 마늘 추출물과는 다른 기전으로 혈소판 응집 억제 효과를 나타내는 menadione과 propranolol도 이와 동일한 결과를 보였다. 실험 조건에 따른 이러한 차이가 PRP 중에 존재하는 알부민 등과의 단백 결합에의한 약물의 유효농도 감소에 기인한다고 추정되어, washed platelet에 여러 농도의 알부민을 첨가하여 혈소판 응집 억제 실험을 실시한 결과 약물의 $IC_{50}$/은 알부민 농도 의존적으로 증가하였다. 혈소판 응집과 함께 혈소판 기능 변화를 측정하는 지표인 혈소판 분비능을 분비된 ATP를 Luciferin-Luciferase로 측정하여 알아보았다. 그 결과 thrombin에 의한 혈소판의 ATP 유리에 미치는 마늘 추출물의 영향을 위의 세 실험조건에서 실험하여 $IC_{50}$/을 비교한 결과 그 값이 washed platelet에서 가장 낮고 PRP에서 가장 높아, 혈소판 응집 억제 실험과 동일한 결과를 나타내었다. 이상의 결과는 세가지 혈소판 분리 조건 중 washed platelet(Ca$^{2+}$비첨가)가 약물에 의한 혈소판 기능 억제 실험에 가장 효율적임을 제시하고 있으며, 그 주요한 원인은 알부민 등 단백 결합에 의한 유효 약물 농도 감소때문 이라고 추정된다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.152-152
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• 1993

혈전 등에 의한 혈행 장애 예방 및 치료 약물의 개발을 목적으로 혈소판 기능에 대한 약물의 영향을 연구하는데 있어, 효율적인 실험 조건의 선택은 중요하다. 본 연구에서는 마늘 메탄올 추출물의 혈소판 응집 억제능을 platelet rich plasma(PRP), washed platelet (Ca$^2$비첨가), washed platelet(Ca$_{2＋}$ 첨가) 등 세가지 분리 조건을 사용하여 실험하였다. 그 결과 마늘 추출물의 $IC_{50}$/이 각각 2348 $\mu\textrm{g}$/ml, 221 $\mu\textrm{g}$/ml, 851 $\mu\textrm{g}$/ml로서, washed platelet(Ca$_{2＋}$ 비첨가)가 마늘 추출물의 혈소판 응집 억제 작용에 가장 민감한 것으로 나타났다. 또한 마늘 추출물과는 다른기전으로 혈소판 응집 억제 효과를 나타내는 menadione과 propranolol도 이와 동일한 결과를 보였다. 실험 조건에 따른 이러한 차이가 PRP 중에 존재하는 알부민 등과의 단백 결합에 의한 약물의 유효농도 감소에 기인한다고 추정되어, washed platelet에 여러 농도의 알부민을 첨가하여 혈소판 응집 억제 실험을 실시한 결과 약물의 $IC_{50}$/은 알부민 농도 의존적으로 증가하였다. 혈소판 응집과 함께 혈소판 기능 변화를 측정하는 지표인 혈소판 분비능을 분비된 ATP를 Luciferin-Luciferase로 측정하여 알아보았다. 그 결과 thrombin에 의한 혈소판의 ATP 유리에 미치는 마늘 추출물의 영향을 위의 세 실험 조건에서 실험하여 $IC_{50}$/을 비교한 결과 그 값이 washed platelet에서 가장 낮고 PRP에서 가장 높아, 혈소판 응집 억제 실험과 동일한 결과를 나타내었다. 이상의 결과는 세가지 혈소판 분리 조건 중 washed platelet(Ca$_{2＋}$ 비첨가)가 약물에 의한 혈소판 기능 억제실험에 가장 효율적임을 제시하고 있으며, 그 주요한 원인은 알부민 등 단백 결합에 의한 유효 약물 농도 감소 때문이라고 추정된다.

• Environmental Analysis Health and Toxicology
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• 제23권4호
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• pp.285-290
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• 2008

Effects of titanium dioxide nanoparticles on blood coagulation and platelet aggregation were investigated using diluted whole blood and platelet rich plasma (PRP) solution prepared from human and rat blood, respectively. Blood coagulation was monitored by using a whole blood impedance aggregometer and titanium dioxide nanoparticle (10, 20, and 40 ppm) did not show any effect on the coagulation both in human and in rat blood. When platelet aggregation was measured by turbidometric method after addition of titanium dioxide nanoparticles to PRP solution with final concentrations of 1, 5 and 10 ppm. no aggregation was observed.