• Title/Summary/Keyword: Plate Spring

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Fluorogenic and Chromogenic Assay for Rapid Detection of Escherichia coli and Total Coliform Bacteria (효소발색법을 이용한 대장균 및 총대장균군 신속 검사)

  • Lee, Keun-Heon;Kim, Hun-Soo;Kim, Byong-Ryol;Lee, Seung-Hui;In, Chi-Kyung;Park, Kyeong-Ryang
    • Journal of Korean Society on Water Environment
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    • v.25 no.3
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    • pp.363-369
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    • 2009
  • We developed the Eco medium for Escherichia coli and total coliforms, which was modified by Violet Red Bile (VRB) medium, and derived the standard curve of exponential phase at $OD_{410}$ by using type strains such as E. coli ATCC11303, Enterobacter cloacae KCTC2361, Klebsiella pneumoniae KCTC2241, and Citrobacter freundii KCTC2359. Also, we used total 93 samples of spring and stream water to compare the detection ability of total coliforms between the method using Eco medium and such as most probable number (MPN), and plate count methods. As a result, the qualitative analysis of E. coli and total coliforms using Eco medium contained ortho-nitrophenyl-$\beta$-galactoside (ONPG) and 4-methylumbelliferyl-$\beta$-D-glucuronide (MUG) was same as those of Korean standard methods (Colilert kit). And the colony forming unit (CFU) detected in Eco medium was similar to those of result from MPN and plate count methods. Moreover, the agreement, sensitivity, and specificity of the developed kit was more than 97.5% in comparison with Colilert kit for 350 samples. Thus, the Eco medium can be used both qualitative and quantitative analysis of E. coli and total coliforms.

Finite Element Modal Analysis of a Spinning Flexible Disk-Spindle System Supported by Hydro Dynamic Bearings and Flexible Supporting Structures In a HDD (유연한 지지 구조와 유체 동압 베어링으로 지지되는 HDD의 회전 유연 디스크-스핀들 시스템에 대한 유한 요소 고유 진동 해석)

  • 한재혁;장건희
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2003.11a
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    • pp.572-578
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    • 2003
  • The free vibration of a spinning flexible disk-spindle system supported by hydro dynamic bearings in a HDD is analyzed by FEM. The spinning flexible disk is described using Kirchhoff plate theory and von Karman non-linear strain, and its rigid body motion is also considered. It is discretized by annular sector element. The rotating spindle which includes the clamp, hub, permanent magnet and yoke, is modeled by Timoshenko beam including the gyroscopic effect. The flexible supporting structure with a complex shape which includes stator core, housing, base plate, sleeve and thrust pad is modeled by using a 4-node tetrahedron element with rotational degrees of freedom to satisfy the geometric compatibility. The dynamic coefficients of HDB are calculated from the HDB analysis program, which solves the perturbed Raynolds equation using FEM. Introducing the virtual nodes and the rigid link constraints defined in the center of HDB, beam elements of the shaft are connected to the solid elements of the sleeve and thrust pad through the spring and damper element. The global matrix equation obtained by assembling the finite element equations of each substructure is transformed to the state-space matrix-vector equation, and the associated eigenvalue problem is solved by using the restarted Arnoldi iteration method. The validity of this research is verified by comparing the numerical results of the natural frequencies with the experimental ones. Also the effect of supporting structures to the natural modes of the total HDD system is rigorously analyzed.

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Development of sit-to-stand assistive chair using a pneumatic cylinder: a feasibility test

  • Hong, SoungKyun;Lee, GyuChang
    • Physical Therapy Rehabilitation Science
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    • v.9 no.3
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    • pp.197-200
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    • 2020
  • Objective: The purpose of this study was to develop and investigate the feasibility of a sit-to-stand assistive chair using a pneumatic cylinder. Design: Cross-sectional study. Methods: The sit-to-stand assistive chair was developed to assist the sit-to-stand movement by rising up of the chair by a pneumatic cylinder. After the user is seated on the chair, if the pneumatic cylinder pulls the seat plate when standing up, the spring of the pneumatic cylinder, which has been stretched, assists in rising the rear end of the seat plate so that the user can stand conveniently and comfortably. A feasibility test was performed in 10 heathy adults. The electromyographic muscle activation of the trunk and lower extremity muscles was analyzed, which included the erector spinae, rectus abdominis, quadriceps, tibialis anterior, gastrocnemius when standing up from sitting using the developed chair and standing up without using the developed chair. Results: As a result, the sit-to-stand assistive chair using a pneumatic cylinder was developed. In the feasibility test, the use of the developed chair had a decrease in rectus abdominis, quadriceps, tibialis anterior activation compared to those who did not use the device in the healthy adults. Conclusions: The sit-to-stand assistive chair using a pneumatic cylinder may be helpful to reduce the activation of the rectus abdominis, quadriceps, tibialis anterior muscles when performing a sit-to-stand movement. Through the results, the efficacy of the sit-to-stand assistive chair can be confirmed. In the future, further studies are warranted to investigate for the safety and efficacy of its use in the elderly population or those who are disabled.

A Practical Analysis Method for the Design of Piled Raft Foundations (말뚝지지 전면기초의 실용적 근사해석법 개발)

  • Song, Young Hun;Song, Myung Jun;Jung, Min Hyung;Park, Yung Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.37 no.1
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    • pp.83-91
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    • 2017
  • In case of estimation of settlement for the piled-raft foundation, it is necessary to consider interaction among raft, piles and soil. But, simple analytic methods usually are not applicable to considering this complicated interaction. In this study, a computer-based approximate analytic method, HDPR, was developed in consideration of above mentioned interaction in order to analysis of settlement for the piled-raft foundation. The finite element method was applied to raft analysis by means of the Mindlin plate theory, and soil and piles were modeled as springs which were connected with their raft. The linear spring which can consider multi layered soil and the non-linear spring were applied to soil springs and pile springs, respectively. The raft-piles-soil interaction was reflected to each spring. In order to verify the developed analytic method, it was compared and analyzed with 3D FEM analysis, existing approximate analytic method and site monitoring data. As a result, the developed analytic method showed reasonable results of settlement estimations of raft and piles for each case. From a practical point of view, it is confirmed that this analytic method is able to apply for analysis and design of the piled-raft foundation.

Performance Evaluation of Micro-nozzle Using Cold Gas Propulsion System (냉가스 추진장치를 이용한 마이크로 노즐의 성능평가)

  • Jung, Sung-Chul;Kim, Youn-Ho;Oh, Hwa-Young;Myong, Rho-Shin;Huh, Hwan-Il
    • Journal of the Korean Society of Propulsion Engineers
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    • v.11 no.6
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    • pp.42-49
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    • 2007
  • In this study, we analyzed flow characteristics of micro-nozzles for basic research to develop micro propulsion system. Cold gas propulsion system was used, and micro-nozzles having nozzle throat diameters of 1.0, 0.5, 0.25 mm were fabricated with EDM method. Thrust was measured through the use of plate-spring and strain gage based thrust measurement system, and flow characteristics of micro-nozzles were analyzed under ambient condition and vacuum condition. We used argon and nitrogen gases as propellant, and compared experimental results with CFD analysis. From the result, we verified the flow losses of viscosity and back-pressure caused by minimization of nozzle.

Survey of bacterial contamination of chicken meat (도계육에 대한 미생물 오염실태 조사)

  • 김선희;나기복;양승민;유제용;배영재;최영태
    • Korean Journal of Veterinary Service
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    • v.26 no.1
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    • pp.27-31
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    • 2003
  • In order to monitoring the chicken meat hygiene, 165 chicken samples were tested for contamination agents such as the total bacterial counts, coliform bacterial counts and falling bacterial counts. The prevalence of level on number of standard plate count(SPC), total bacterial counts in less than 10$^3$cfu/$\textrm{cm}^2$, 10$^3$∼10$^4$cfu/$\textrm{cm}^2$ and 10$^4$∼10$\^$5/cfu/$\textrm{cm}^2$ were 30 samples (18.2%), 98 samples(59.4%) and 37 samples(22.4%), respectively. The coliform bacterial counts in less than 10$^2$cfu/$\textrm{cm}^2$, 10$^2$∼10$^3$cfu/$\textrm{cm}^2$ and 10$^3$∼10$^4$cfu/$\textrm{cm}^2$ were 7 samples(4.2%), 66 samples (40.0%), 92 samples(55.8%), respectively. The falling bacterial counts in plants were higher 9.1, in summer 8.4, in fall 7.2 in spring and 6.7 in winter decreased.

Non-linear analysis of pile groups subjected to lateral loads using 'p-y' curve

  • Chore, H.S.;Ingle, R.K.;Sawant, V.A.
    • Interaction and multiscale mechanics
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    • v.5 no.1
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    • pp.57-73
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    • 2012
  • The paper presents the analysis of two groups of piles subjected to lateral loads incorporating the non-linear behaviour of soil. The finite element method is adopted for carrying out the parametric study of the pile groups. The pile is idealized as a one dimensional beam element, the pile cap as two dimensional plate elements and the soil as non-linear elastic springs using the p-y curves developed by Georgiadis et al. (1992). Two groups of piles, embedded in a cohesive soil, involving two and three piles in series and parallel arrangement thereof are considered. The response of the pile groups is found to be significantly affected by the parameters such as the spacing between the piles, the number of piles in a group and the orientation of the lateral load. The non-linear response of the system is, further, compared with the one by Chore et al. (2012) obtained by the analysis of a system to the present one, except that the soil is assumed to be linear elastic. From the comparison, it is observed that the non-linearity of soil is found to increase the top displacement of the pile group in the range of 66.4%-145.6%, while decreasing the fixed moments in the range of 2% to 20% and the positive moments in the range of 54% to 57%.

Rocking Stiffness of Electrical Cabinet for In-Cabinet Response Spectrum (캐비닛내부응답스펙트럼을 위한 전기캐비닛 전도강성)

  • Chung, Yon Ha;Hong, Kee-Jeung;Cho, Sung Gook
    • Journal of the Earthquake Engineering Society of Korea
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    • v.24 no.2
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    • pp.95-102
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    • 2020
  • Electrical instruments and devices contained in cabinets for controlling nuclear power plants require seismic qualification; likewise, in-cabinet response spectrum (ICRS) is necessary. Gupta et al. (1999) suggested the Ritz method, where rocking, frame bending, and plate bending behaviors of cabinets are considered, as a method for determining ICRS. This research proposes a method to determine the rocking stiffness of cabinets, which represents its rocking behavior. The cabinet is fixed on mounting frames and is connected to the base concrete by anchors. When horizontal excitation is applied to the cabinet, the mounting frames at anchors are locally deformed, the mounting frames are bent, and then rocking in the cabinet becomes evident. A method to determine equivalent vertical spring stiffness representing the local deformation of the mounting frames at anchors is then proposed. Subsequently, the rocking stiffness of this mounting frame is calculated upon assumption of the mounting frame as an indeterminate beam.

Regional variations of optical properties on asteroid (25143) Itokawa taken with the Asteroid Multi-band Imaging Camera (AMICA) on-board the Hayabusa spacecraft

  • Lee, Mingyeong;Ishiguro, Masateru
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.2
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    • pp.45.1-45.1
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    • 2015
  • Hayabusa is the JAXA's space mission that succeeded in sample-return from S-type asteroid (25143) Itokawa. During the rendezvous phase, more than a thousand of images were taken with the Asteroid Multi-band Imaging Camera (AMICA). It is valuable to study the regional variation of the optical properties on the asteroid using these images to know the generality and uniqueness of the returned samples. In addition, AMICA images are important in that they provide unique data set at low phase angle (i.e Sun-Itokawa-AMICA's angle) that have not been explored in the previous asteroidal missions. At the previous conference (2015 KAS spring meeting), we introduced our preliminary data analysis of AMICA data without considering the shape model of Itokawa and mentioned. In this study, we present a new result obtained through further analysis, taking account of the shape model of the asteroid. We thus utilized "plate_renderer" tool to derive Hapke model parameters at different terrains. It is found that the opposition amplitude (parameter B0) is consistent with those of the other S-type asteroids while the opposition width (parameter h) is significantly narrower than those of the other S-type asteroids. At this conference, we plan to describe the regional variation of photometric properties on Itokawa.

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Application of the Laplace transformation for the analysis of viscoelastic composite laminates based on equivalent single-layer theories

  • Sy, Ngoc Nguyen;Lee, Jaehun;Cho, Maenghyo
    • International Journal of Aeronautical and Space Sciences
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    • v.13 no.4
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    • pp.458-467
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    • 2012
  • In this study, the linear viscoelastic response of a rectangular laminated plate is investigated. The viscoelastic properties, expressed by two basic spring-dashpot models, that is Kelvin and Maxwell models, is assumed in the range to investigate the influence of viscoelastic coefficients to mechanical behavior. In the present study, viscoelastic responses are performed for two popular equivalent single-layered theories, such as the first-order shear deformation theory (FSDT) and third-order shear deformation theory (TSDT). Compliance and relaxation modulus of time-dependent viscoelastic behavior are approximately determined by Prony series. The constitutive equation for linear viscoelastic material as the Boltzmann superposition integral equation is simplified by the convolution theorem of Laplace transformation to avoid direct time integration as well as to improve both accuracy and computational efficiency. The viscoelastic responses of composite laminates in the real time domain are obtained by applying the inverse Laplace transformation. The numerical results of viscoelastic phenomena such as creep, cyclic creep and recovery creep are presented.