• Geomechanics and Engineering
• /
• v.23 no.3
• /
• pp.245-259
• /
• 2020

Tunnels have become an indispensable part of metro cities. Blast resistance design of tunnel has attracted the attention of researchers due to numerous implosion event. Present paper deals with the non-linear finite element analysis of rock tunnel having shear zone subjected to internal blast loading. Abaqus Explicit schemes in finite element has been used for the simulation of internal blast event. Structural discontinuity i.e., shear zone has been assumed passing the tunnel cross-section in the vertical direction and consist of Highly Weathered Granite medium surrounding the tunnel. Mohr-Coulomb constitutive material model has been considered for modelling the Highly Weathered Granite and the shear zone material. Concrete Damage Plasticity (CDP), Johnson-Cook (J-C), Jones-Wilkins-Lee (JWL) equation of state models are used for concrete, steel reinforcement and Trinitrotoluene (TNT) simulation respectively. The Coupled-Eulerian-Lagrangian (CEL) method of modelling for TNT explosive and air inside the tunnel has been adopted in this study. The CEL method incorporates the large deformations for which the traditional finite element analysis cannot be used. Shear zone orientations of 0°, 15°, 30°, 45°, 60°, 75° and 90°, with respect to the tunnel axis are considered to see their effect. It has been concluded that 60° orientation of shear zone presents the most critical situation.

• The Korean Journal of Physiology and Pharmacology
• /
• v.19 no.2
• /
• pp.105-109
• /
• 2015

NgR1, a Nogo receptor, is involved in inhibition of neurite outgrowth and axonal regeneration and regulation of synaptic plasticity. P19 embryonal carcinoma cells were induced to differentiate into neuron-like cells using all trans-retinoic acid and the presence and/or function of cellular molecules, such as NgR1, NMDA receptors and STAT3, were examined. Neuronally differentiated P19 cells expressed the mRNA and protein of NgR1, which could stimulate the phosphorylation of STAT3 when activated by Nogo-P4 peptide, an active segment of Nogo-66. During the whole period of differentiation, mRNAs of all of the NMDA receptor subtypes tested (NR1, NR2A-2D) were consistently expressed, which meant that neuronally differentiated P19 cells maintained some characteristics of neurons, especially central nervous system neurons. Our results suggests that neuronally differentiated P19 cells expressing NgR1 may be an efficient and convenient in vitro model for studying the molecular mechanism of cellular events that involve NgR1 and its binding partners, and for screening compounds that activate or inhibit NgR1.

• Geomechanics and Engineering
• /
• v.9 no.4
• /
• pp.531-545
• /
• 2015

Two fundamental issues exist in the damage theory of geo-material based on the concept of thermodynamics: existence or nonexistence of the dissipation potential, and whether the dissipation potential could be decoupled into a damage potential and a plastic one or not. Thermodynamics theory of elastoplastic damage assumes the existence of dissipation potential, but the presence of dissipation potential is conditional. Based on the dissipation inequality in accord with the second law of thermodynamics, the sufficient and necessary conditions are given for the existence of the dissipation potential separately in total and incremental forms firstly, and proved strictly in theory. With taking advantage of the basic mechanical properties of geo-materials, the nonexistence of the dissipative potential is verified. The sufficient and necessary conditions are also given and proved for the decoupling of the dissipation potential of geo-materials in total and incremental forms. Similarly, the non-decoupling of the dissipation potential has also been proved, which indicates the dissipation potential of geo-materials in total or incremental forms could not be decoupled into a dissipative potential for plasticity and that for damage respectively. The research results for the fundamental issues in the thermodynamics theory of damage will help establish and improve the theoretic basis of elastoplastic damage constitutive model for geo-materials.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.97-97
• /
• 2003

Embryonic stem (ES) cells, derived from preimplantation embryos, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES (hES, MB03) cells and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2 O_2$. Ratio of dying cells as determined by the relative amount of dye neutral red entrapped within the cells after the exposures. Cell death rates were not significantly different when either MB03 or HeLa were exposed up to 0.4 mM $H_2 O_2$. However, relative amount of dye entrapped within the cells sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2 O_2$, while it was approximately 54% in MB03. Pretreatment of cells with BSO (GSH chelator) and measurement of GSH content results suggest that cellular GSH is the major defensive mechanism of hES cells. Induction of apoptosis in hES cell was confirmed by DNA laddering, induction of Bax, and chromatin condensation. In summary, hES cells 1) are extremely resistant to oxidative stress, 2) utilize GSH as a major defensive mechanism. and 3) experience apoptosis upon exposure to oxidative stress.

• Transactions of Materials Processing
• /
• v.16 no.7
• /
• pp.530-537
• /
• 2007

This paper is concerned with the analysis of plastic deformation of bimetal co-extrusion process. Two sets of material combination have been adopted for analysis, i.e. combinations of Cu/Al and Fe/Al. In the first set of material combination, the selected materials are AA 1100 aluminum alloy as hard material and CDA 110 as soft one. This type of material selection is to examine the effect of hard core and soft sleeve and vice versa on the deformation pattern in terms of plastic zone and velocity discontinuity along the contact surface between construction materials. Four different cases of co-extrusion process in terms of material combination and interference bonding were simulated to investigate the effect of material arrangement between core and sleeve, and of bonding on the plastic zones and velocity discontinuity. In the other set of material combination, model materials used as core and sleeve were AA 1100 and AISI 1010, which are relatively soft and hard, respectively. Process parameters except diameter ratio of core to sleeve material such as semi-die angle, reduction in area in global sense and die comer radius have been set constant throughout the simulation to concentrate our effort on the analysis of influence of diameter ratio on deformation behavior such as deformation zone, surface expansion, exit velocity discontinuity between composite materials, and extrusion forces.

• Journal of Ocean Engineering and Technology
• /
• v.25 no.3
• /
• pp.66-72
• /
• 2011

This is the fourth of a series of companion papers dealing with the mechanical property reductions of various marine structural steels. Even though a reduction of the elastic modulus according to temperature increases has not been obtained from experiments, high temperature experiments from room temperature to $900^{\circ}C$ revealed that initial the yield strength and tensile strength are both seriously degraded. The mechanical properties obtained from high temperature experiments are compared with those from EC3 (Eurocode 3). It is found that the high temperature test results generally comply with the prediction values by EC3. Based on the prediction of EC3, time domain nonlinear finite element analyses were carried out for a blast wall installed on a real FPSO. After applying the reduced mechanical properties, corresponding to $600^{\circ}C$ to the FE model of the blast wall, more than three times the deflections were observed and it was observed that most structural parts experience plastic deformations exceeding the reduced yield strength at the high temperature. It is noted that a protection facility such as PFP (passive fire protection) should be required for structures likely to be directly exposed to fire and explosion accident.

• Composites Research
• /
• v.12 no.4
• /
• pp.42-54
• /
• 1999

FMLC(Fiber-Metal Laminate Composites) is a new structural material combining thin metal laminate with adhesive fiber prepreg, it nearly include all the advantage of metallic materials, for example: good plasticity, impact resistance, processibility, light weight and excellent fatigue properties. This research studied the optimum design of the FMLC subject to various loading conditions using genetic algorithm. The finite element method based on the shear deformation theory was used for the analysis of FMLC. Tasi-Hill failure criterion and Miser yield criterion were taken as fitness functions of the fiber prepreg and the metal laminate, respectively. The design variables were fiber orientation angles. In genetic algorithm, the tournament selection and the uniform crossover method were used. The elitist model was also used to be effective evolution strategy and the creeping random search method was adopted in order to approach a solution with high accuracy. Optimization results were given for various loading conditions and compared with CFRP(Carbon Fiber Reinforced Plastic). The results show that the FMLC is more excellent than the CFRP in point and uniform loading conditions and it is more stable to unexpected loading because the deviation of failure index is smaller than that of CFRP.

• Proceedings of the KSAR Conference
• /
• 2003.06a
• /
• pp.78-78
• /
• 2003

Embryonic stem (ES) cells, derived from preimplantation embryo, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In contrast, terminally differentiated cells do not usually alter their nature but frequently die or transform if they are exposed to inappropriate external stimulations. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES cells (MB03) and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2O$$_2$. Approximately 1$\times$10$^4$ cells were plated in 96 well plate and serum starved for overnight. The conditioned cells were exposed to a various concentration of $H_2O$$_2$ fur 24 hrs and loaded with neutral red (50$\mu\textrm{g}$/ml) for 4 hrs, washed with PBS for 2 min three times, and entrapped dye was dissolved out using acetic ethanol. Cytotoxicity was determined by reading the amount of dye in the medium using microplate reader. equipped with 575 nm filter. Relative amount of the dye entrapped within MB03 or HeLa were not significantly different when cells were exposed up to 0.4 mM $H_2O$$_2$. However, this sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2O$$_2$, while it was approximately 54% in MB03 suggesting that this concentration of $H_2O$$_2$ is the defensive threshold for HeLa cells. The resistance to oxidative stimulation reversed, however, when cells were co-treated with BSO (L-buthionine- 〔S, R〕-sulfoximine) which chelates intracellular GSH. This result suggests that cellular GSH is the major defensive mechanism of human ES cells. Induction of enzymes involved in GSH metabolism and type of cell death is currently being studied.

• Advances in concrete construction
• /
• v.6 no.3
• /
• pp.245-268
• /
• 2018

The numerical investigations have been carried out on deep beam with opening subjected to static monotonic loading to demonstrate the accuracy and effectiveness of the finite element based numerical models. The simulations were carried out through finite element program ABAQUS/CAE and the results thus obtained were validated with the experiments available in literature. Six simply supported beams were modelled with two square openings of 200 and 250 mm sides considered as opening at centre, top and bottom of the beam. In order to define the material behaviour of concrete and reinforcing steel bar the Concrete Damaged Plasticity model and Johnson-Cook material parameters available in literature were employed. The numerical results were compared with the experiments in terms of ultimate failure load, displacement and von-Mises stresses. In addition to that, seventeen beams were simulated under static loading for studying the effect of opening location, size and shape of the opening and depth, span and shear span to depth ratio of the deep beam. In general, the numerical results accurately predicted the pattern of deformation and displacement and found in good agreement with the experiments. It was concluded that the structural response of deep beam was primarily dependent on the degree of interruption of the natural load path. An increase in opening size from 200 to 250 mm size resulted in an average shear strength reduction of 35%. The deep beams having circular openings undergo lesser deflection and thus they are preferable than square openings. An increase in depth from 500 mm to 550 mm resulted in 78% reduced deflection.

• Transactions of Materials Processing
• /
• v.23 no.8
• /
• pp.501-506
• /
• 2014

Progressive shearing with blanking dies is commonly employed to produce large quantities of tiny sheet metal electronic parts. Sheet metal pins, which are narrow and long, that are sheared with a progressive die set are often twisted. The twist in the sheet metal pins, which usually occurs in the final shearing operation, generally decreases with increasing blank holding force. The blank holding forces in all shearing operations are not the same because of different shearing positions and areas. In the current study, the optimal layout of the springs in a progressive die set to minimize the twist of the sheet metal pin is proposed. In order to find the holding force acting on the tiny narrow blanks produced with the proposed springs during the shearing process, the equivalent area method is used in the structural analysis. The shearing of the sheet-metal pin was simulated to compute the twist angle associated with the blank holding force. The constraint condition satisfying the pre-set blank holding force from the previous shearing operations was imposed. A design of experiments (DOE) was numerically implemented by analyzing the progressive die structure and by simulating the shearing process. From the meta-model created from the experimental results and by using a quadratic response surface method (PQRSM), the optimal layout of the springs was determined. The twist of sheet metal pin associated with the optimal layout of the springs found in the current study was compared with that of an existing progressive die to obtain a minimal amount of twist.