• 치위생과학회지
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• 제11권4호
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• pp.375-380
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• 2011

This study evaluated whether fluoride treatment can affect recovery of the irregularity of enamel surface after tooth whitening with a high concentration of hydrogen peroxide (HP) activated by plasma arc light. A total of 36 bovine teeth stained with coke were used in this experiment. The specimens were classified into following three groups (two different commercial plasma arc groups and a control group without light curing source): (1) 35% HP gel only, (2): 35% HP gel and Plasma arc A, and (3) 35% HP gel and Plasma arc B. To measure color changes and surface morphologies before and after the bleaching, colorimeter and scanning electron microscopy were used, respectively. When the specimens were bleached with hydrogen peroxide and plasma arc lights, the bleaching effect was greater than when only hydrogen peroxide gels were used (Kruskal-Wallis test, p<0.05). In addition, plasma arc B showed the more color changes than plasma arc A (Bonferroni post-hoc test, p<0.05). The surfaces of the teeth treated with fluoride gel after the whitening treatment came to be smooth. Therefore, the results of this study suggested that the fluoride application for patients who got tooth whitening therapy with a high concentration of hydrogen peroxide gels activated by plasma arc light will be effective to recover rough enamel surfaces.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.250.2-250.2
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• 2014

Currently, teeth whitening method which is applicable to dental surgery is that physician expertises give medical treatment to teeth directly dealed with a high concentration of hydrogen peroxide and carbamide peroxide. If hydrogen peroxide concentration is too high for treatment of maximized teeth whitening effect [1], it is harmful to the human body [2]. To the maximum effective and no harmful teeth whitening effect in a short period of time at home, we have observed the whitening effect using carbamide peroxide (15%) and a low-temperature atmospheric pressure plasma jet which is regulated by the Food and Drug Administration. The gas supplied conditions of the non-thermal atmospheric pressure plasma jet was with the humidified (0.6%) gas in nitrogen or air at gas flow rate of 1000 sccm. Also, the measurement of chemical species from the jet was carried out using the optical emission spectroscopy (OES), the evidence of increased reactive oxygen species compared to non-humidified plasma jet. We have found that the whitening effect of the plasma is very excellent through this experiment, when bovine teeth are treated in carbamide peroxide (15%) and water vapor (0.2 to 1%). The brightness of whitening teeth was increased up to 2 times longer in the CIE chromaticity coordinates. The colorimetric spectrometer (CM-3500d) can measure color degree of whitening effect.

• 식물병연구
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• 제28권4호
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• pp.204-208
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• 2022

새싹인삼의 뿌리썩음병 억제제로서 과산화수소의 활용 가능성을 구명하고자 항진균 활성효과와 묘삼 소독효과를 연구한 결과, 과산화수소는 300 mg/l의 농도에서 1×10⁷ conidia/ml 농도의 Cylindrocarpon destructans 균총을 80% 이상 억제시켜 항진균 활성을 나타내었고 인위적으로 병원균에 감염시킨 묘삼의 유병율을 20% 이하로 억제시켜 소독효과가 매우 높았다. 결과적으로 과산화수소 희석수 300 mg/l를 14일에 걸쳐 분무처리하였을 때, 인위적으로 병원균에 감염된 새싹인삼의 뿌리썩음병 발생수율이 72.1%로 무처리의 발생수율 87.2%에 비해 다소 억제되었다. 그러나, 과산화수소의 주기적인 살포가 새싹인삼의 생육 부진을 다소 유발하였다. 추가 연구로서 뿌리썩음병의 방제에 효과적이고 새싹인삼에 안전한 과산화수소의 처리방법 개발이 요구되었다.

• International Journal of Oral Biology
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• 제39권4호
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• pp.187-192
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• 2014

Nonthermal atmospheric pressure plasma has attracted great interest for biomedical applications. The plasma consists of charged particles, radicals, and a strong electric field as the fourth state of matter. This study evaluated the change in the surface roughness after tooth bleaching by plasma in combination with a low concentration (15%) of carbamide peroxide, specifically whether the application of plasma produced detriments, such as demineralization and structural change, with the goal of efficient and safe tooth bleaching. After being combined with plasma and 15% carbamide peroxide, the hydroxyapatite surface was significantly smoother with a low roughness average value. Tooth bleaching with 15% carbamide peroxide alone produced an irregular surface and increased the surface roughness with high roughness average value. Tooth bleaching with plasma resulted in no significant variations in hydroxyapatite in terms of change in surface roughness and surface topography. The application of tooth bleaching with plasma is not deleterious to dental hard tissue, implicating it as a safe tooth bleaching technique.

• 치위생과학회지
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• 제9권5호
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• pp.525-530
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• 2009

이 연구는 35% 과산화요소를 이용한 전문가 치아미백술에 플라즈마 아크를 함께 적용하여 플라즈마 아크가 법랑질의 색조변화에 미치는 효과와 비커스경도와 주사전자현미경을 이용하여 법랑질 표면에 미치는 영향을 알아보고자 in vitro 평가를 시행하여 다음과 같은 결론을 얻었다. 1. 미백 횟수의 증가에 따라 색조변화량(${\Delta}E^*$)이 통계적으로 유의한 증가를 나타내었으며, 미백 제만 단독으로 적용한 대조군 보다 플라즈마 아프를 함께 적용하여 미백처리를 한 실험군의 색조변화량(${\Delta}E^*$)이 더 높게 나타나는 것을 알 수 있었다. 2. 미백처리 전 후 법랑질의 미세경도 측정 값을 분석한 결과 미백 처치를 함으로써 미세경도 값이 통계적으로 유의하게 감소되었고, 미백제에 플라즈마 아크를 함께 적용한 실험군에서 더 큰 미세경도의 감소가 나타나는 것을 알 수 있었다. 3. 주사전자현미경 관찰에서 미백 처치를 한 시료에서 법랑질 표면이 상당히 거칠어진 양상을 나타내는 것을 볼 수 있었다. 이상의 결과를 종합해 볼 때 전문가 치아미백술에서 플라즈마 아크를 함께 적용함으로써 색조변화를 더욱 상승시킬 수 있으나, 법랑질 표면의 미세경도와 형태변화를 증가시킬 수 있으므로 미백 효과는 증가시키면서 법랑질의 형태와 구조는 변화시키지 않는 안전한 미백 방법에 관한 연구가 이루어 져야 할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권10호
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• pp.1373-1376
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• 2000

The peroxidation status of tissues was estimated in broilers under acute or chronic heat stress ($32^{\circ}C$, 24 h, $5{\times}24h$) in the present study. The results showed that the lipid peroxide (LPO) concentrations in plasma and liver were elevated (p<0.05) by acute heat stress, and were not influenced in kidney (p>0.05). At the same time, no significant change of superoxide dismutase (SOD) activity in the liver, kidney or plasma was observed. Under chronic heat exposure, the SOD activity in liver was increased (p<0.05) and the LPO concentrations in the liver and plasma were restored to the normal levels. The LPO level in kidney was not affected by chronic heat stress (p>0.05), but SOD activity was significantly decreased (p<0.01). The results suggested that the peroxidation was induced by acute heat stress and disappeared along with the time of heat exposure, and the peroxidation reactions were different among tissues.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.172-172
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• 2012

Non-thermal plasma has attracted medical researchers, since they showed higher apoptosis rate in cancer cells than normal cells. However, it is hard to conclude general cancer cell specific effect because comparison between normal and cancer cell activities after plasma treatment have not been reported yet. This research proposes a comparison of Dielectric Barrier Discharge (DBD) plasma effect on three normal cells lines and three cancer cells lines. We measured cell number, mitochondria activity (MTS assay) and amount of hydrogen peroxide (H2O2) for three days. The results show that the number of cancer cells decreased more than normal cells following of exposure time. On the other hand, mitochondria activities and amounts of H2O2 increased following of exposure time. In addition, we found that DBD plasma exposure on cell suspension in media and media only illustrated no difference in mitochondria activity, H2O2 quantity, and cell number. Thus, we can confirm higher apoptosis rate in cancer cells which is related to the reactive oxygen species (ROS) generated by DBD plasma. The related molecular mechanisms were investigated further.

• Journal of Nutrition and Health
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• 제31권4호
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• pp.687-696
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• 1998

This study was performed to investigate effects of hesperidin and naringin on linpid peroxide formation and antioxidative enzyme activities in rats. Thiobarbituric acid reactive substance (TBARS) concentrations were measured in plasma and liver. Catalase, superoxide dismutase, and glutathione peroxidase activities were measured in erythrocyte and liver. Forty-nine male Sprague-Dauley rats weighing 275.3$\pm$3.3g were blocked into seven groups according to body weight and were raised fro four weeks on diets containing 0.25, 0.50 or 1.00%(w/w) hesperidin or naringin . Food intake, weight gain , food efficiency ratio, and weights of liver, kidney, spleen ,and epididymal fat pad were not significantly different among groups. In 0.50 and 1.00% naringin groups , plasma TBARS concentrations were significantly decreased with a dose response patter. In 0.25, 0.50 and 1.00% hesperidin groups, liver TBARS concentrations were significantly decreased without a dose dependent patter. Antiosidative enzyme activities in erythrocyte and liver were not significantly affected by type and amountof dietary bioflavonoid, but in the 1.00% hesperidin group, catalase, superoxide dismutase, and glutahione perosidase activities in linver showed a tendency to increase. In conclusion, naringin inhibited lipid peroxide formation with a dose response pattern in plasma without changing the activities of antioxidative enzymes. Hesperidin adminstration, regardless of the level in the diet, inhibited lipid peroxide formation in liver.

• Journal of Animal Science and Technology
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• 제47권1호
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• pp.29-38
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• 2005

Effect of dietary brown seaweed(Undaria pinnatifida) levels on the anti-oxidant enzyme system was evaluated in blood of broiler chicks activated innate immune response. Day-old broiler chicks were fed diets containing 0.0(basal), 1.0, 2.0 and 4.0 % of brown seaweed for 4 weeks. The innate immune response was activated by injecting Salmonella typhymurium lipopolysaccharide(LPS) i.p. at 8, 10 and 12 day of age. The activation of innate immune response enhanced(p< 0.01) and the brown seaweed 1.0 and 2.0 % diets reduced(P< 0.05) the superoxide dismutase(SOD) activity in erythrocyte cytosol significantly. The activation of innate immune response elevated significantly the levels of peroxide and the activity of peroxidase in plasma, while the levels of dietary brown seaweed resulted in a significant linear increase in peroxidase activity in plasma of normal bird. Experience of the innate immune response in 4 week-old chicks reduced linearly the plasma level of peroxide with the level of brown seaweed and enhanced the plasma peroxidase activity. Also, the plasma of normal birds fed the brown seaweed showed higher level of peroxide and lower activity of peroxidase. The results indicated that dietary brown seaweed affected SOD and peroxidase activities in blood of broiler chicks during activation of innate immune response.

• 대한의생명과학회지
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• 제12권4호
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• pp.355-359
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• 2006

This investigation was performed to study the antioxidant activities of Cornus officinalis Sieb extracts and the effect of Cornus officinalis Sieb extracts on glucose, lipid metabolism in diabetic rats. DPPH free radical scavanging activity and superoxide anion radical scavenging of Cornus officinalis Sieb extracts were 94.7% and 92.1%, respectively. Streptozotocin (45 mg/kg body weight, i.p.) induced diabetic rats showed a significant increases of plasma glucose, triglyceride and total cholesterol, concomitantly significant decrease of plasma high density lipoprotein. Glutathione level were decrease in cytosol of liver, lung and brain tissue of rats. Lipid peroxide were increase in microsome of liver cells. Group 1 and 2 were treated with Cornus officinalis Sieb extracts 200 mg/kg body weight and 100 mg/kg body weight for 24 days, individually. Group 1 and 2 rats showed decreased plasma glucose, triglyceride, total cholesterol and lipid peroxide in microsome of liver, and increased plasma high density lipoprotein and glutathione in cytosol of liver, lung and brain. The result suggest that Cornus officinalis Sieb extracts may normalize the Impaired antioxiants status in streptozotocin induced diabetic rats. Cornus officinalis Sieb extracts were used to improve the imbalance between free radicals and antioxidant system due to the diabetes.