• 한국임상수의학회지
• /
• 제22권3호
• /
• pp.253-258
• /
• 2005

The aim of this study was to estimate the time of ovulation and mating derived by plasma progesterone and estradiol-$17{\beta}$ concentrations. The 11 pregnant Shih-tzu bitches were investigated the plasma progesterone and estradiol-$17{\beta}$ concentrations from proestrus to parturition. Gestation length in the 11 pregnant bitches was $61.9{\pm}1.1\;(mean{\pm}SD)$ days when Day 0 was timed from the day that plasma progesterone concentration was first increased above 4.0 ng/ml. The litter size was $3.8{\pm}0.3$ pups. Plasma progesterone concentrations were increased at the first day of vulvar bleeding and showed at Day 0 with $5.2{\pm}0.3$ ng/ml. It was gradually increased to reach a peak at Day 15 with $42.6{\pm}3.7ng/ml$, thereafter it was gradually decreased to below Day 62. Plasma estradiol-$17{\beta}$ concentrations were increased above 1.0 pg/ml at the first day of vulvar bleeding and showed a peak at Day -2 with $33.5{\pm}8.0$ pg/ml, thereafter it was gradually decreased. When Day 0 was timed from the day that plasma progesterone concentration was first increased above 4.0 ng/ml, plasma estradiol-$17{\beta}$ concentration reached a peak at Day -2. In conclusion, these results indicated that ovulation was estimated to occur the day when plasma progesterone concentration was first increased above 4.0 ng/ml after the first day of bleeding. It was estimated that mating time was the day when plasma progesterone concentration was between $3.0{\~}8.0$ ng/ml.

• 대한한방내과학회지
• /
• 제16권1호
• /
• pp.181-196
• /
• 1995

The present experiments were designed to investigate the effects of BangPoongSan on the cardiovascular system in the experimental Animals. And thus the change of blood pressure, auricular blood flow, artery contraction, death rate, platelet aggregation repression, plasma coagulation factor activity, plasma antithrombin activity, whole blood viscosity and plasma viscosity were studied. The result were summarized as the followings: 1. BangPoongSan dropped the blood pressure in the spontaneous hypertensive rat. 2. The drug increased the auricular blood flow in rabbit. 3. The drug relaxed the artery contraction by pretreated norepinephrine in white rat. 4. The drug inhibited the death rate of mouse which was led to thromboembolism by serotonin and collagen. 5. The drug inhibited the platelet aggregation in rat. 6. The drug prolonged the prothrombin time and activated partial thromboplastin time on the test of plasma coagulation factor activity in rat, but was not valuable. 7. The drug presented the antithrombin activity in rat. 8. The drug reduced the whole blood viscosity and plasma viscosity in rat, but the latter was not valuable. According to the results, Bangpoongsan increased the blood flow and dropped the blood pressure by dilatation of blood vessel smooth muscle. And the drug presented the antithrombin acivity, inhibited the platelet aggregation and reduced blood viscosity. Therefore these effects are assumed to improve the cardiovascular circulation disorder and prevent thrombosis.

• Fisheries and Aquatic Sciences
• /
• 제20권3호
• /
• pp.4.1-4.6
• /
• 2017

Juvenile Anoplopoma fimbria (mean length $16.8{\pm}2.2cm$, and mean weight $72.8{\pm}5.4g$) were exposed for 2 months with different levels of ammonia (0, 0.25, 0.50, 0.75, 1.00, and 1.25 mg/L). Growth performances such as daily length gain, daily weight gain, condition factor, and hepatosomatic index were significantly decreased by ammonia exposure. Hematological parameters such as red blood cell (RBC) count, hematocrit, and hemoglobin were also significantly decreased. In plasma inorganic components, calcium and magnesium were significantly decreased by ammonia exposure. In plasma organic components, there was no alteration in cholesterol and total protein. In enzyme plasma components, glutamic oxalate transaminase (GOT) and glutamic pyruvate transaminase (GPT) were significantly increased. The results of this study indicated that ammonia exposure can induce significant growth reduction and blood biochemistry alterations of A. fimbria.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권7호
• /
• pp.954-959
• /
• 2004

In a herd of 24 spring-born steers, plasma leptin and performance of selected purebred (n=5) and backcrossed Hereford (n=5) were compared in a year-round summer grazing and winter feedlot fattening. Bimonthly blood collection and body weight measurement were accomplished. The plasma samples were analyzed for leptin, insulin, total cholesterol, triglyceride, NEFA and glucose. The experimental design utilized one-way ANOVA with breed as the treatment. The purebred obtained higher plasma NEFA (p<0.001) compared to backcross, regardless of seasonal feeding systems (SFS). The backcross showed gradual increase and nonresponsiveness of plasma leptin to SFS. During summer grazing, attenuation of plasma leptin and sudden elevation when shifted to winter feedlot fattening were observed in purebred. Plasma leptin obtained linear relationship with body weight of purebred (r=0.53;p<0.001) and backcrossed Hereford (r=0.49; p<0.01). The purebred and backcrossed Hereford, when shifted to summer grazing, resulted to sustained and restricted daily gain, respectively. Therefore, cattle breeds of higher growth potential exhibit significant elevation of plasma leptin after 400 kg BW, when animal starts to deposit significant body fat.

• 대한의생명과학회지
• /
• 제7권3호
• /
• pp.151-159
• /
• 2001

Many prospective studies have confirmed the predictive value of plasma fibrinogen levels for vascular diseases, including ischemic heart disease. Several polymorphisms of the $\beta$fibrinogen gene have been investigated in relation to plasma fibrinogen levels. The blood samples for DNA were collected from 109 healthy Koreans who have no relationship by blood (67 males and 42 females) in due consideration of some other factors such as gender, age, and smoking status. Four polymorphisms of the $\beta$fibrinogen gene that consist of HaeIII, AluI, MaII and BcII restriction fragment length polymorphisms (RFLPs) were investigated to examine the associations between RFLPs and plasma fibrinogen levels. In conclusion, the significant associations between HaeIII, AluI, MnII RFLPs(H$_1$H$_2$, M$_1$M$_2$, $A_1$A$_2$) and the concentration of plasma fibrinogen were shown by the smokers as well as by the old people more than 50, whereas the association between BcII and plasma fibrinogen were shown no connection with the status of age and smoking. The concentration of plasma fibrinogen was significantly shown higher by the old people ($\geqq$50) by the younger people ($\leqq$49) in male and also higher by the smokers than by the nonsmokers.

• 대한예방한의학회지
• /
• 제8권1호
• /
• pp.75-87
• /
• 2004

Objectives: Rubi Fructus(fruit of Rubus coreanus Miq.) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Rubi Fructus(fruit of Rubus coreanus Miq.) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat erythrocytes and blood plasma. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Rubi Fructus (fruit of Rubus coreanus Miq.) administered group(D-galactose 50mg/kg and Rubi Fructus(fruit of Rubus coreanus Miq.) extracts 85.0mg/200g 1time/day for 6 weeks, BBJ Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : Plasma TBARS concentrations of all experimental group were not significantly different. Red blood cell(RBC) SOD activities of BBJ group was increased, and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of BBJ group was increased. Plasma total lipid concentration of BBJ group were significantly lower than those of control. Plasma triglyceride, total cholesterol and HDL-cholesterol concentrations of all experimental group were not signi ficantly different. Conclusions: According to the above results, it is considered that Rubi Fructus(fruit of Rubus coreanus Miq.) is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권1호
• /
• pp.47-51
• /
• 2010

The objective of this study was to understand changes of plasma metabolites, hormones, and mRNA level of cytoplasmic phosphoenolpyruvate carboxykinase (PEPCK-C) in liver in spontaneous clinical ketosis; 10 clinically ketotic cows and 10 healthy cows were chosen from the same dairy farm. Eleven blood parameters and liver fat content were measured in all cows, and mRNA levels of PEPCK-C in liver were measured by semi-quantitative reverse transcription (RT) polymerase chain reaction (PCR). In ketotic cows, concentration of plasma glucose decreased (p<0.01), concentration of plasma nonesterified fatty acids (NEFA) and $\beta$-hydroxybutyric acid (BHBA) increased (p<0.01), liver fat content (18.8% wet weight) and activity of plasma aspartate aminotransferase (AST) increased (p<0.01), but concentration of plasma total bilirubin (TBIL), $\gamma$-glutamyl transpeptidase ($\gamma$-GT), and cholinesterase (CHE) increased (p>0.05). In addition, concentration of plasma insulin decreased (p<0.05), concentration of plasma glucagons decreased (p>0.05), and mRNA level of PEPCK-C in liver increased (p<0.05). It is concluded that the adaptative changes of metabolites, hormones, and mRNA level of PEPCK-C in ketotic cows were in favor of the enhancement of gluconeogenesis, the decrease of fat mobilization and the relief of ketosis, but these were still inadequate to relieve ketosis.

• 한방재활의학과학회지
• /
• 제20권3호
• /
• pp.27-35
• /
• 2010

Objectives : The present study investigated anti-inflammatory effect of Artemisia Capilaris Thunberg in lipopolysaccharide-exposed rats. Methods : We divided lipopolysaccharide-exposed Sprague-Dawley rats into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 6 weeks. We measured counts of red blood cell(RBC), the values of hemoglobin(Hb) and packed cell volume(PCV), plasma total protein concentration, albumin concentration, the ratio of albumin/globulin, the activities of plasma glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase(GPT), lactate dehydrogenase(LDH), the counts of white blood cell(WBC), the ratio of neutrophils, lymphocytes, monocytes, basophils, eosinophils, the concentration of plasma interleukin-$1{\beta}$($IL-1{\beta}$), plasma interleukin-6(IL-6), plasma tumor necrosis factor-$\alpha$($TNF-{\alpha}$), plasma interleukin-10(IL-10), the concentration of liver $IL-1{\beta}$ and IL-6, $TNF-{\alpha}$, IL-10. Results : Counts of RBC and the values of Hb and PCV, plasma total protein concentration and albumin concentration, the activities of plasma GOT, GPT and LDH showed no significant difference in the treatment groups. and the ratio of albumin/globulin was increased in Artemisia Capillaris Thunberg groups. The counts of WBC showed lower values in Artemisia Capillaris Thunberg groups than those of control group, In the ratio of neutrophils Thunberg groups. The ration of monocytes, basophils and eosinophils were below 5%, and showed no characteristic trend. The concentration of plasma interleukin-$1{\beta}$($IL-1{\beta}$), plasma inerleukin-6(IL-6) and plasma tumor necrosis factor-$\alpha$($TNF-{\alpha}$) showed a lower values in the Artemisia Capillaris Thunberg groups than those of control group, and the concentration of plasma interleukin-10(IL-10) showed no significant difference in the treatment groups. The concentration of liver $IL-1{\beta}$ and IL-6 showed a lower values in the Artemisia Capillaris Thunberg groups than those of control group, however the concentration of liver $TNF-{\alpha}$ and IL-10 showed no significant difference in the treatment groups. Conclusions : The Artemisia Capillaris Thunberg groups gives positive results of anti-inflammatory response by lipopolysaccharide(LPS) derivation.

• 대한수의학회지
• /
• 제39권2호
• /
• pp.267-275
• /
• 1999

Bovine somatotropin is known to improve the growth rate and lactation in cattle. In this study, we examined the concentration-time profiles of a sustained-release formulation of bovine somatotropin (BST) and insulin-like growth factor-1 (IGF-1) in plasma and milk in cows. In addition, the possible effect of co-administrated vitamin ADE complex on the pharmacokinetic parameters of BST and IGF-1 was evaluated. 1. Plasma BST and IGF-1 levels reached the peak at 12~24 and 48 hours after the administration of BST, and plasma half-lives ranged 100 to 137 and 201 to 310 hours, respectively. To 8th day after administration, BST and IGF-1 levels in milk were not significantly different from the control levels. 2. Plasma BST levels showed cyclic pattern with high concentrations in early stage after each injection and following gradual declining during repeated administrations at 2 week intervals, while plasma IGF-1 levels in treated animals did not show such a cyclic pattern, but remained higher than the control levels. 3. Milk BST and IGF-1 levels during repeated treatments were not significantly different from the control levels. 4. Co-administration of vitamin ADE complex yielded slightly increased AUC of plasma BST for high dose group, but such effect was not evident in the IGF-1 levels. Co-administration of ADE complex tended to increase plasma BST levels and decrease the elimination half-life of IGF-1. 5. These results suggest that the BST formulation tested is one of the ideal sustained-release formulation for long term use in dairy industry. As for the co-administration of vitamin ADE complex, the benefit of co-administration with BST is needed to be further evaluated.

• 대한핵의학회지
• /
• 제6권1호
• /
• pp.17-24
• /
• 1972

Utilizing the commercial radioimmunoassay kit, the author assayed HGH and evaluated the problems of the method. The method had the sensitivity of 0.5 mug/ml degree and could determine the plasma HGH concentration directly without the help of plasma extraction. Also it was specific for the HGH, when tested by a dilution method using test utilizing the plasma of the acromegalic patient. The author also obtained the $^{125}I$ labelled HGH of specific activity of $156.3{\mu}Ci/{\mu}g$, performing the chloramine-T method.