• Journal of Microbiology and Biotechnology
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• 제29권9호
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• pp.1401-1411
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• 2019

Mycobacterial cell walls comprise thick and diverse lipids and glycolipids that act as a permeability barrier to antibiotics or other chemical agents. The use of OH radicals from a non-thermal plasma jet (NTPJ) for the inactivation of mycobacteria in aqueous solution was adopted as a novel approach. Addition of water vapor in a nitrogen plasma jet generated OH radicals, which converted to hydrogen peroxide ($H_2O_2$) that inactivated non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis H37Rv. A stable plasma plume was obtained from a nitrogen plasma jet with 1.91 W of power, killing Escherichia coli and mycobacteria effectively, whereas addition of catalase decreased the effects of the former. Mycobacteria were more resistant than E. coli to NTPJ treatment. Plasma treatment enhanced intracellular ROS production and upregulation of genes related to ROS stress responses (thiolrelated oxidoreductases, such as SseA and DoxX, and ferric uptake regulator furA). Morphological changes of M. smegmatis and M. tuberculosis H37Rv were observed after 5 min treatment with $N_2+H_2O$ plasma, but not of pre-incubated sample with catalase. This finding indicates that the bactericidal efficacy of NTPJ is related to the toxicity of OH and $H_2O_2$ radicals in cells. Therefore, our study suggests that NTPJ treatment may effectively control pulmonary infections caused by M. tuberculosis and nontuberculous mycobacteria (NTM) such as M. avium or M. abscessus in water.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2012년도 춘계학술대회 논문집
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• pp.115-116
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• 2012

• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.161.2-161.2
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• 2015

Melanin is a black pigment, responsible for hair and skin color. In order to find the melanin stimulatory technique which prove useful for a gray and a white hair-preventive agent or tanning agent, we developed atmospheric pressure plasma jet (APPJ) and tested for tyrosinase activity and melanin production in melanoma (B16F10) cells in vitro. We found plasma dose dependent increase in melanin production. To explore the contributing mechanism in melanin synthesis, intracellular reactive oxygen species (ROS) and MAP kinase signaling pathways were studied. Furthermore, the development of plasma technology for melanin synthesis and planning for in-vivo future studies will be discussed.

• 한국항공우주학회지
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• 제45권11호
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• pp.907-913
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• 2017

아크 플라즈마에 의해 구동되는 스파크 제트의 다양한 에너지 공급 방법에 따른 효율적 운전 성능 특성에 대한 실험적 연구를 수행하였다. 펄스 당 37 mJ의 주입 에너지에 의한 급속한 기체의 가열에 의해 약 330 m/s의 고속 제트가 발생함을 확인하였다. 제트의 최대 속도와 침투 거리는 각각 주입된 전력량과 펄스 당 주입된 에너지에 비례하였다. 낮은 에너지에서는 오리피스 직경이 작을수록 더 높은 속도의 제트가 발생하였다. 공급 에너지가 같다면 전류를 높인 펄스가 펄스 폭을 높인 펄스보다 높은 속도의 제트를 발생시켰다. 펄스 폭이 약 $10{\mu}s$이고 펄스 당 에너지가 약 10 mJ인 경우가 효율적인 운전에 보다 더 적합한 것으로 확인되었다.

• 대한기계학회논문집B
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• 제23권5호
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• pp.593-602
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• 1999

This paper presents combustion characteristics of LPG-air mixture ignited by the plasma jet in a cylindrical vessel with constant volume, in which our focus is placed on the multi-hole plug configuration. Four types of the plug configuration depending on the number of orifice and the arranged angle are considered, along with two cases of conventional spark ignition for comparison. Not only the flame propagation is photographed at intervals, but the pressure in the combustion chamber is also recorded through the entire combustion process. The results show that the plasma jet ignition enhances the overall combustion rate remarkably in comparison to the spark ignition by generating irregular flame front and penetrating through the unburned mixture. The combustion enhancement rate agrees favorably with the available data, which supports the validity of our experiment. Synthetically estimating, the two-hole sixty-degree plug appears to be the most desirable, in that the maximum pressure as well as the combustion duration is less affected by the sub-energy level than the others. It is also deduced that there may exist an optimal plug configuration capable of rapid combustion for a specific combustion chamber.

• 한국전산유체공학회지
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• 제17권2호
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• pp.85-92
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• 2012

Flows over a square cylinder with and without plasma actuation are numerically investigated to see whether plasma actuation can effectively modify vortex shedding from the cylinder and reduce the drag and lift fluctuations. In this study, a plasma synthetic jet actuator is mounted on the rear side of cylinder as a means of direct-wake control. The effect of plasma actuation is considered by adding a momentum forcing term in the Navier-Stokes equations. Results show that the reduction of mean drag and lift fluctuations is obtained for both steady and unsteady actuation. However, the steady actuation is better than the unsteady one in terms of mean drag as well as drag fluctuations. With the strong steady actuation considered, the interaction of two separating shear layers from rear corners is effectively weakened due to the interference of synthetic jets. It results in a merging of synthetic-jet and shear-layer vortices and the increase of vortex shedding frequency. On the other hand, the unsteady actuation generates pulsating synthetic jets in the near wake, but it does not change the vortex shedding frequency for the actuation frequencies considered in this study.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2009년도 제40회 하계학술대회
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• pp.1533_1534
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• 2009

This paper presents an atmospheric micro glow plasma-jet device. The device consists of four components; a thin Ni anode, a porous alumina insulater, a stainless steel cathode and an aluminum case. The Ni anode is fabricated using micromachining technology. The anode has 10 holes, of which the hole diameter and the depth are $250{\mu}m$ and $60{\mu}m$, respectively. The discharge test is performed in nitrogen gas at atmospheric pressure for 20 kHz AC bias. The breakdown voltage is 3.5 kV at gas flow rate of 4 L/min and the the plasma-jet is blown out to ambient at 5.5 kV. In order to verify the characteristics of plasma, the current and the voltage of device are measured. The maximum temperature of plasma is $37^{\circ}C$. The plasma is well generated and stable at high voltage.

• 한국추진공학회지
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• 제21권6호
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• pp.57-63
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• 2017

본 연구에서는 플라즈마 분사장치를 활용하여 항력감소를 위한 기초 실험을 수행하였다. 가시화 장비와 항력 측정 장비를 이용하여 기초 실험 장치를 구성하였다. 자유유동 환경에서 분사되는 플라즈마 역분사 제트를 가시화하기 위한 방법으로 쉴리렌 기법을 활용하였다. 가시화 실험 결과를 통해 플라즈마 제트의 침투와 유동구조 변화를 관찰하였다. 항력감소 가능성을 측정하기 위한 방법으로 로드셀을 이용한 측정을 수행하였다. 그 결과 초음속 자유유동 조건에서 역으로 분사되는 플라즈마를 통해 항력이 6.2% 감소함을 보였다.

• Applied Science and Convergence Technology
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• 제26권6호
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• pp.201-207
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• 2017

An electron-excited temperature ($T_{ex}$) is not determined by the Boltzmann plots only with the spectral data of $4p{\rightarrow}4s$ in an Ar-plasma jet operated with a low frequency of several tens of kHz and the low voltage of a few kV, while $T_{ex}$ can be obtained at least with the presence of a high energy-level transition ($5p{\rightarrow}4s$) in the high-voltage operation of 8 kV. The optical intensities of most spectra that are measured according to the voltage and the measuring position of the plasma column increase or decay exponentially at the same rate as that of the intensity variation; therefore, the excitation temperature is estimated by comparing the relative optical-intensity to that of a high voltage. In the low-voltage range of an Ar-jet operation, the electron-excitation temperature is estimated as being from 0.61 eV to 0.67 eV, and the corresponding radical density of the Ar-4p state is in the order of $10^{10}{\sim}10^{11}cm^{-3}$. The variation of the excitation temperature is almost linear in relation to the operation voltage and the position of the plasma plume, meaning that the variation rates of the electron-excitation temperature are 0.03 eV/kV for the voltage and 0.075 eV/cm along the plasma plume.

• 한국항공우주학회지
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• 제47권2호
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• pp.90-97
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• 2019

스파크제트 액츄에이터는 플라즈마 합성 제트 액츄에이터(plasma synthetic jet actuator, PSJA)라고도 불리는 능동 유동 제어 장치로, 초음속 유동의 제어 가능성이 있어 많은 연구가 진행 중이다. 이 액츄에이터는 아크 플라즈마를 이용해 캐비티(cavity) 내부에 에너지를 주입하여 온도와 압력을 상승시킨다. 온도와 압력이 상승한 캐비티에서 오리피스(orifice)를 통해 압력파와 제트가 분출되어 외부 유동에 교란을 준다. 플라즈마의 영향으로 캐비티 유동은 고온, 고압의 평형 유동이 되기 때문에 스파크제트 액츄에이터의 유동 해석을 위해선 공기의 평형 상태를 고려해야 한다. 본 연구에서는 평형 유동의 특성을 고려하여 스파크제트 액츄에이터 유동 해석을 위한 수치해석 프로그램을 개발했다. 개발된 프로그램의 검증으로 문헌에서 얻을 수 있는 실험 결과와 시간에 따른 제트의 위치를 비교했다. 또한 상온, 상압의 무풍에서 액츄에이터의 추력 특성을 분석했다.