• 대한한방내과학회지
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• 제11권2호
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• pp.43-52
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• 1990

The following results were obtained from the observation on the change of plasma cortisol concentration and arterial blood $PO_2,\;PCO_2$ in the experiment of intravenous and oral administration of Chung Sang Bo Ha Tang Water Extract in the rabbit. 1. In intravenous administration, the plasma cortisol conce-ntration increased significantly about 2 hours after with a dose of $0.2m{\ell}/kg$, while the case of $0.4m{\ell}/kg$ reveals this significant increase of concentration about 3 hours after. 2. In oral administration, a significant increase of the plasma cortisol concentration was shown about 2 and 4 hours after with a dose of $1.2m{\ell}/kg$, on the other hand, an increase of concentration was shown at each hour in the case of $0.2m{\ell}/kg$, but it was not significant. 3. In oral administration, $PO_2$ in arterial blood increased significantly in the case of $0.2m{\ell}/kg$, and both the case of $0.1m{\ell}/kg$ and that of $0.2m{\ell}/kg$ gave a significant decrease of $PCO_2$ in arterial blood. With these results, it is verified that Chung Sang Bo Ha Tang has therapeutic effects on JISU(止嗽), JEONG CHEON(定喘), GEO DAM(祛痰) because intravenous and oral administration of it increases the plasma cortisol concentration, However, in the examination of $PO_2$ and $PCO_2$ in arterial blood, any remarkable result was not obtained.

• Journal of Veterinary Science
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• 제24권2호
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• pp.33.1-33.7
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• 2023

Background: Laminitis is a complex and debilitating disease of horses. Numerous predisposing factors contribute to laminitis development, however the exact pathogenesis remains undetermined. Serum T4, cortisol, and histamine are components of the innate stress response and could play a causative or contributory role. Stress hormone concentrations in laminitis are largely unknown. Objective: To evaluate parameters associated with stress response in horses with laminitis, and compare these to healthy horses and horses with gastrointestinal (GI) disease. Methods: Thirty-eight adult horses presenting for non-medical conditions, GI abnormalities, or clinical laminitis were prospectively enrolled. Horses were assigned to the appropriate disease group (healthy, GI disease, and laminitis) and had blood drawn on presentation to the hospital. Samples were analyzed for plasma endogenous adrenocorticotrophic hormone (eACTH), serum cortisol, serum thyroid hormone, and plasma histamine. Results: Stress hormone concentrations were significantly different between horses in the laminitis and GI disease groups. Plasma histamine levels were highest in horses with laminitis, compared with GI disease and controls. Both horses with laminitis and GI disease had increased plasma eACTH when compared to healthy horses. Horses with GI disease had higher serum cortisol concentrations than horses with laminitis or controls. Serum T4 was lower in horses with GI disease than in horses with laminitis and controls. Conclusions: Horses with laminitis had relative increases in both plasma histamine and eACTH concentrations. Serum T4 and cortisol concentrations of horses with laminitis did not differ significantly when compared to healthy horses. The role of stress hormones in equine disease warrants further investigation.

• 한국해양생명과학회지
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• 제4권2호
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• pp.91-95
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• 2019

잉어, Cyprinus carpio를 대상으로 고수온 스트레스 발생 시 코티졸 호르몬 변화 및 누적 사망률을 조사하였다. 수온 변화에 따른 20℃, 24℃ 및 28℃ 실험군의 코티졸 변화는 수온 변화 후 6시간에 코티졸 함량이 증가하다가 24시간 이후부터 감소하여 20℃ 및 24℃ 실험군은 실험 전의 코티졸 함량에 가까운 수치로 감소하였다. 48시간의 28℃ 실험군 코티졸 함량은 실험 전의 코티졸 함량보다 높았으나 24시간의 코티졸 함량보다는 유의한 차이를 보이며 감소하였다. 반면, 32℃ 실험군은 수온 변화 후 6시간부터 코티졸 함량이 증가하여 24시간에 코티졸 함량이 최대치로 증가하였으며 48시간까지 코티졸 함량이 감소하지 않고 유지되는 경향이 나타났다. 20℃, 24℃ 및 28℃ 실험군은 수온 변화 후 12시간까지 누적 사망률이 증가하다가 그 이후부터 사망하는 개체가 발생하지 않았으나, 32℃ 실험군은 실험 종료 시(48시간)까지 지속적으로 누적 사망률이 증가하는 경향이 나타났다. 본 연구로 도출된 결과를 이용하여 호르몬 분석을 통해 특정지역의 폐사 위험 정도를 확인할 수 있는 방안으로 활용 가능할 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.202-203
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• 1989

• 대한한방내과학회지
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• 제13권1호
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• pp.143-155
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• 1992

The following results were obtained from the observation on the change of plasma cortisol concentration in the experiment of intravenous administration of Haengsoyeum Water Extract in the rabbit. And the effects of Haengsoyeum extract on the contractile force of the isolated guinea pig trachea smooth muscle. 1. In intravenous administration the plasma cortisol concentration increased significantly about 1 hours after with a does of 0.2 ml/kg. 2. In intravenous administration the plasma cortisol concentration increased significantly about from 1 to 3 hours after with a does of 0.4 ml/kg. 3. The contractile response of the trachea smooth muscle of isolated guinea pig to histamine $10^{-4}\;M$ was significantly inhibited by Haengsoyeum extract. 4. The contractile response of the trachea smooth muscle of isolated guinea pig to acetylcholine $10^{-4}\;M$ was considerably inhibited by Haengsoyeum extract. 5. The contractile response of the trachea smooth muscle of isolated guinea pig to 5-hydroxytryptamine $10^{-4}\;M$ was inhibited by Haengsoyeum extract.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1430-1434
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• 2005

We conducted two experiments to assess the effect of transfer from housing to grazing on stress hormone secretion in cattle using urine samples. In a preliminary experiment, urine samples were collected following an adrenocorticotrophic hormone (ACTH) challenge, and cortisol levels in urine were compared with the levels in plasma. In a second experiment, urinary cortisol was measured before and after the start of a grazing season in 6 Japanese Shorthorn cows, all of which had experienced grazing before. In experiment 1, urinary cortisol showed a pattern of changes similar to that of plasma with a 0.5-h temporal lag time, and the peak levels were 4 to 10 times higher than the basal levels. In experiment 2, the urinary cortisol levels in cows did not change after the cows were let out to pasture, with no decreases in body weight. This study suggests that the transfer from housing to grazing did not affect physiological responses to cause high excretion of urinary cortisol in grazing-experienced cattle using a non-invasive sampling method.

• Fisheries and Aquatic Sciences
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• 제26권2호
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• pp.117-132
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• 2023

Chum salmon (Oncorhynchus keta) is a species of anadromous salmonid inhabiting coastal rivers in the North Pacific and the Arctic in the Bering and is the most widely distributed among Pacific salmon species. It is an important fish species in Korea as the salmon releasing project is being actively carried out. This study was conducted to investigate changes in the physiological activity of O. keta according to water temperature. Three experiments were conducted according to the water temperature and period, and the plasma concentrations of cortisol and glucose were analyzed from the blood samples of the experimental groups. Experiment I is a short-term water temperature experiment, in which water temperature stimulation was given for 4 hours at water temperatures of 12℃, 16℃ (control), 20℃, and 24℃, and a recovery period was given for 4 hours. Experiment II is an experiment in which water temperature stimulation was given for 24 hours, 48 hours, and 72 hours at a high temperature of 24℃, and a recovery period was given for 12 hours, respectively. Experiment III is a long-term water temperature experiment, in which the water temperature was 12℃, 16℃ (control), 20℃, and 24℃ for 8 weeks. As a result of the experiment, in Experiment I, there was no significant difference in the survival rate between the experimental groups, but the concentration of cortisol and glucose in the plasma according to the set water temperature showed a significant difference. In Experiment II, there was no significant trend according to the maintenance time of the high-temperature state, but as the temperature increased, the plasma cortisol and glucose levels significantly increased compared to the control group. In Experiment III, all of the experimental group C (24℃) died in the 1st week, and there was no significant difference in the plasma glucose at the 1st and 8th weeks among the remaining experimental groups.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.87-89
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• 1993

Cortisol levels in plasma are known to be as an indication of reproductive and adrenal status of an animal. In this study it has been examined in relation to the oestrus induction by Progesterone oestrogen therapy in 3rd and 4th parity anoestrus animals. Cortisol was found higher in treated animals and levels raised within 6-12 hrs. after hormone therapy followed by elevation in glucose levels and depletion of total serum proteins. It shows the association of induction, occurrence and expression of oestrus with energy demanding metabolic stress in buffaloes.

• 환경위생공학
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• 제12권2호
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• pp.37-42
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• 1997

The toxic and detoxifying effects of cadmium and zinc on rat plasma ACTH and serum cortisol levels were investigated in rats. Rats were injected by i.p. with saline (0.9%), cadmium chloride (0.25 or 0.5mg/kg body weight) and pretreated with zinc chloride (4mg/kg body weight) before cadmium chloride treatment 1 or 2 weeks, respectively. The ACTH levels were no significant differences in cadmium 0.25mg/kg-treated group, but were significantly decreased in cadmium 0.5mg/kg-treated group compared with normal group. The ACTH levels after zinc pretreatment for 1 week were significantly increased but zinc pretreatment for 2 week were no difference. The serum cortisol levels of cadmium treated rats were significantly decreased, but were increased in zinc pretreated rats. The results showed that the zinc have some protective effect on cadmium toxicity in rats.

• 한국양식학회지
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• 제19권4호
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• pp.315-322
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• 2006

본 연구에서는 담수사육 감성돔을 대상으로 수온을 상승시켰을 때, 세포적 스트레스 측면에서 HSP90 및 HSP70 mRNA의 발현 정도를, 신경-내분비적 스트레스 측면에서 혈장 cortisol 및 glucose 농도를 조사하였다. RT-PCR법을 이용하여 생식소로부터 HSP90 (891 bp) 및 HSP70 (465 bp) cDNA 단편을 클로닝 하여, 타 종과 그 상동성을 비교해 본 결과, 감성돔 HSP90은 참돔 HSP90과 99%, 무지개송어 HSP90과 95%, 대서양 연어HSP90과 94%, zebrafish HSP90과 94%로 나타났으며, 감성돔 HSP70은 무지개송어 HSP70과 96%, silver seabream HSP70과 95%, zebrafish HSP70과 95%의 상동성을 나타내었다. 감성돔의 사육수온을 $30\;^{\circ}C$로 상승시켰을 때, HSP90 mRNA는 모든 조직에서 그 발현 정도가 $20\;^{\circ}C$ 실험구에 비하여 $7{\sim}9$배 정도 높았으나, HSP70 mRNA는 생식소에서만 발현하는 것으로 나타났다. 혈장 cortisol 및 glucose 농도는 $20\;^{\circ}C$ 실험구에 비하여 $30\;^{\circ}C$ 실험구에서 유의하게 증가한 것으로 나타났다.