• Journal of Plant Biotechnology
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• 제31권1호
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• pp.61-65
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• 2004

본 실험은 Alocasia cadieri Chantrier를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Alocasia의 경정을 TDZ 0.1mg/L가 첨가된 배지에 배양하였을 때 신초증식이 가장 높았으며, 형성된 신초에서 신초의 증식은 TDZ과 NAA가 각각 0.5mg/L첨가된 배지에서 효과적이었다. 형성된 신초의 발근은 IBA또는 NAA가 첨가된 배지보다는 활성탄 2.0g/L가 첨가된 배지에서 양호하였으며, 소식물체의 순화는 perlite : vermiculite가 1:1로 혼합된 용토 또는 vermiculite가 적합하였다.

• Current Research on Agriculture and Life Sciences
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• 제31권2호
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• pp.94-100
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• 2013

An efficient plant regeneration protocol is developed for a standard-type chrysanthemum. When petal segments derived from flower buds (4 or 8cm in diameter) were used as the culture material, the highest shoot regeneration frequency (96%) was obtained on a Murashige and Skoog (MS) medium supplemented with 0.5 mg/L IAA, 2 mg/L BA, 3% sucrose, and a 0.8% agar. Pre-culturing the explants under dark conditions for 14 days produced better results for the shoot regeneration frequency than the explants cultured under a continuous 16 h photoperiod ($40{\mu}molm^{-2}s^{-1}$). The shoot regeneration frequency ranged from 19.0% for the Shinmato cultivar to 89.1% for the Baeksun cultivar. Activated charcoal (0.2%) enhanced the root formation of the regenerated shoots in a hormone-free MS medium. The rooted plantlets were acclimatized and successfully established in a greenhouse.

• 한국약용작물학회지
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• 제12권5호
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• pp.384-390
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• 2004

An in vtro nucellar polyembryo propagation method was established with mature seed of the Citrus junos Sieb. 7-8 nucellar polyembryos per seed were induced on MS basal medium without plant growth regulators. The polyembryos developed to complete plantlets on teatment with IBA. These shoots grew further in MS medium without plant growth regulators. Rooting of shoots occurred on MS medium supplemented with IBA. These plantlets were successfully transplanted to small plastic pot containing soil mixture. Somatic embryos were induced from nucellar polyembryo and maturation occurred spontaneously from proliferating cultures on MS medium without growth regulators. Random Amplified Polymorphic DNA (RAPD) marker analysis of in vitro and in vivo grown junos orange showed identical polymorphism indicative of their genetic stability. The RAPD polymorphism produced revealed same banding pattern in each regenerant. Hence, propagaton of junos orange by nucellalr polyembryos was efficient and produced in genetically stable plants under in vitro conditions.

• Journal of Forest and Environmental Science
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• 제30권4호
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• pp.405-411
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• 2014

Wild Korean ginseng has been recognized as highly precious medicine since ancient times. Nowadays, the population of wild ginseng in the forest of Korean peninsula is very rare due to indiscreet harvest. In this work, we investigated the plant regeneration via somatic embryogenesis from embryogenic callus of old wild ginseng (more than 50 years-old) and compared the features of plants regenerated from 5-years old and 50 years-old ginseng. Induction of embryogenic callus from adventitious roots of 50 year-old wild ginseng required 83 weeks of culture, but only 10 weeks were sufficient for 5 year-old ginseng. Height and width of plants derived from the old wild ginseng was smaller and slender compared to the plantlets derived from 5 year-old ginseng. Total chlorophyll contents was 2-6 time lower in plantlets regenerated from 50 year-old wild ginseng than those from 5 year-old ginseng, but anthocyanin content was higher in 50 year-old ginseng. Our results revealed that plants regenerated from old wild ginseng have different morphological and physiological characters probably due to age-dependent phenomenon.

• 한국환경과학회지
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• 제25권3호
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• pp.337-343
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• 2016

The present study aimed to determine the influence of various root restriction media on seedling quality and early growth of strawberry after transplanting. The root activity of the seedlings, measured 20 days after fixation, was considerably higher (0.096, 0.090, and $0.063mg{\cdot}g^{-1}{\cdot}h^{-1}$ at 420, 450, and 480 nm, respectively) in expanded rice hull (ERH) treatment than in the sandy loam and loamy sand treatments. The volumetric water content (VWC) of the root media tested across 3 irrigation regimes (15 d, 30 d, 45 d) in the nursery field was highest in sandy loam (65.0-66.8%), followed by 59.4-61.3% in loamy sand and 38.6-45.3% in ERH. When growth parameters of runner plantlets were compared, ERH treatment was found to result in the highest crown thickness and fresh weights of root and above-ground parts. This had a favorable influence on above-ground tissue growth after transplanting to plastic house soil. As mentioned above, ERH treatment resulted in the highest seedling quality and early growth after transplanting. The results of this study would serve as useful on-site data for the production of high-quality strawberry seedlings.

• 식물조직배양학회지
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• 제22권2호
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• pp.115-120
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• 1995

국내품종인 동진벼와 태백벼의 미숙접합자배 유래 배발생 현탁배양 세포의 초저온 보존 시스템을 개발하였다. 동결/해동 후 캘러스 재생률은 동진벼의 경우 2 M DMSO와 0.4 M sucrose를 태백벼의 경우 0.64 M DMSO와 0.4 M sucrose를 혼용처리하였을 때 캘러스 재생률이 각가 88%와 90%로 가장 높았다. 또한 고농도의 삼투용액에서 배양세포 의 전처리 과정은 필요하지 않았다. 재생된 캘러스를 1 mg/L NAA와 5 mg/L kinetin이 첨가 된 $N_{6 }$, 배지로 이식하여 명배양하였을 때 체세포배발생을 통하여 다수의 유식물체가 발달하였다. 약 100여개의 식물체가 재분화되었으며, 이중 25%는 albino이었다.다.

• Journal of Plant Biotechnology
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• 제30권3호
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• pp.275-279
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• 2003

Excised cotyledons and embryo axises of zygotic embryos of Rhus vemicifera were cultured on Murashige and Skoog(MS) medium with various concentrations of 2,4-D. About 3-5% of explants produced callus. Embryogenic callus was preferentially induced from basal parts of embryo axis of zygotic embryos seeds when they were cultured without removal of seed coats. Somatic embryos were developed from embryogenic callus in growth regulator-free medium after 2-3 subcultures on medium with 1.0mg/L 2,4-D and these embryos were matured to cotyledonary stage. Plantlets with well-developed shoots and roots from embryos were obtained on $\frac{1}{4}$MS medium with GA$_{3}$. After acclimatization of plantlets on artificial soil, they were exposed to soil pots.

• 한국약용작물학회지
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• 제14권4호
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• pp.225-228
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• 2006

식물생장조절제의 종류와 농도를 모색하여 할미꽃의 발근 최적 조건을 조사하여 정상적인 재분화체를 획득하고자 하였다. 지금까지의 선행연구를 토대로 최적조건에서 shoot를 유도시킨 후 발근 유도 생장조절제에 바로 옮기면 shoot가 너무 작아 재분화체가 성장하지 못하고 고사되었다. 1/2 MS에서 키운 shoot를 3.0 mg/L NAA를 첨가한 배지에서 3일간 생장시킨 후, 1/3 MS로 옮겨 치상 한 경우, 약 10일 전후에 뿌리의 유도가 관찰되었다. 이렇게 뿌리가 유도된 재분화체는 염의 농도와 설탕의 농도를 동시에 순차적으로 낮추어 순화처리되었다.

• 한국산림과학회지
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• 제82권3호
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• pp.254-259
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• 1993

이병묘로부터 마이코플라즈마 무병주의 생산법을 확립하기 위하여 대추나무 시험관묘의 액아에서 생장점을 채취하여 기내접목을 실시하였다. 대목으로는 기내에서 발아시킨 대추나무 실생묘의 배축을 사용하였다. 접수중 10%(30점 중 3점씩 2회)가 정상적으로 생장하였고 계속적인 증식과 발근이 가능하여 유식물체로 분화되었다. 마이코플라즈마의 존재여부를 전자현미경과 형광현미경 검경방법을 이용하여 검경한 결과, 접수로 사용한 시험관묘에서는 마이코플라즈마가 관찰되었으나 접목묘에서는 관찰되지 않았다. 이로써 마이코플라즈마를 보균한 시험관묘의 생장점을 접수로 한 기내 미세접목을 통하여 건전묘 생산의 가능성을 보여주었다.

• Journal of Crop Science and Biotechnology
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• 제10권3호
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• pp.141-146
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• 2007

The roots of Aristolochia elegans Mast.(Aristolochiaceae) are widely used in Mexican traditional medicine as a remedy for scorpion venom. Current experimental evidence supports its purported antidote properties. However, collection from the wilderness has lead to local extinction of natural populations. In order to contribute to species preservation, cultivation, and standardization of morphological and pharmacological properties, a micropropagation method was developed. This includes in-vitro germination of seeds to produce aseptic plantlets, induction of multiple budding, and acclimatization. The treatment with benzylamino purine(10 ${\mu}M$) induced the highest number of buds(3.1 on average) in both types of explants. On the other hand, indolebutyric acid(1.5 ${\mu}M$) caused the highest root index(11.8) per explant. One hundred percent of the micropropagated plantlets developed vigorously after the acclimatization process.