• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.207-212
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• 2022

The optimal culture conditions for root organogenesis from the callus of peonies (Paeonia lactiflora Pall.) were investigated. Root explants with vascular bundles were cultured in Murashige and Skoog (MS) medium combined with 0.5-4.0 mg/L auxins (indole acetic acid [IAA], naphthalene acetic acid [NAA], indolebutyric acid [IBA], and 2,4-dichlorophenoxyacetic acid [2,4-D]) and 0.0-2.0 mg/L cytokinins (kinetin, zeatin, and benzylaminopurine [BAP]) to induce callus formation. The callus was then cultured in MS medium combined with three concentrations (0.1, 0.5, and 1.0 mg/L) of IAA, NAA, IBA, kinetin, zeatin, and BAP in the dark for 6 weeks. Based on the results, the effects of dark and light conditions on the callus cultured in MS medium with combinations of 0.1-1.0 mg/L IBA and zeatin for 6 weeks were studied. Callus formation was most effective (>+++) in the medium with a combination of 1.0 mg/L NAA and 1.0 mg/L zeatin. A high number of long adventitious roots were observed in the mediums with 0.1 mg/L IBA (6.66 and 4.82 cm) and 0.5 mg/L zeatin (2.32 and 0.72 cm) among auxins and cytokinins, respectively. The highest number (14.06) of adventitious roots were formed from the callus cultured in light in the MS medium combined with 0.1 mg/L IBA and 0.5 mg/L zeatin. This same medium induced the formation of the longest adventitious root (5.45 cm) in the dark. Thus, optimization of in vitro culture conditions may be possible for the mass propagation of adventitious roots in peonies.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.131-132
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• 2003

Leaf discs and apical meristems were cultured in Murashige and Skoog (MS) medium supplemented with cytokinin and auxin at different concentrations. Callus production was observed in all tested media after six days of incubation. Callus produced in the presence of high concentration of NAA (2.0mg/1) was fragile in texture and yellow in colour. Highest callus formation was observed from leaf discs in the medium supplemented with 1.0mg/1 NAA and 0.5 mg/l BAP in dark at $25{\pm}1{\circ}C$. Percentage of callus formation was 95% and mean callus fresh weight was 654.88 43.53 mg. Shoots were induced from the callus after 4 weeks in 1/2MS medium supplemented with BAP and kinetin both at 0.5mg/1. When elongated shoots were separated and transferred into multiplication medium (MS+0.5mg/1 BAP+0.5mg/1 kinetin) multiplication rate was 6.4 after 6 weeks. Higher concentrations of BAP caused callus production at the base. Direct shoot induction was observed from apical meristems in MS medium in the presence of 0.175 mg/1 IAA + 2.25mg/1 BAP and 0.175 mg/1 IAA + 3.0 mg/1 BAP in 16 hour day at $25{\pm}1{\circ}C$. Explants (apical meristems) elongated to form a single shoot forming a callus at the base. Adventitious buds were sprouted out from the base. Percentage explants which producing shoots was 28.57 and 65.5 respectively. Multiple shoot induction was also observed in the same media. Highest multiple shoot production was observed in the presence of 0.175 mg/l IAA and 3.0mg/l BAP, Mean number of shoots per explant was 5.36 and the mean shoot length was $16.66{\pm}4.15$mm. Shoots (20 30m length) were tested for root induction. Excised shoots were transferred into rooting media, which contains different concentrations of NAA and IAA. Best rooting performance was observed in 1/2MS medium supplemented with 0.1mg/1 NAA after 10 days of incubation in 16 hr photoperiod at $25{\pm}1{\circ}C$. Mean number of roots per shoot was 6 and the mean root length was 252mm. Rooted plantlets were transferred into sterile coir dust:sand (1:4) mixture and maintained in a humid chamber for two weeks, They were gradually exposed to the natural environment. After three weeks they were transferred to pots containing coir dust:sand (1:2) mixture for further development where the 90% survival was observed.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.318-318
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• 2022

Basil(Ocimum basilicum L.) is an annual herb that grows wild in hot and humid regions such as tropical Asia and Africa. This study was carried out for resource discovery, propagation, and DB construction of aromatic plants. In order to evaluate the genetic characteristics, 28 kinds of basil seeds were supplied from the Center for Genetic Resources. For basil characteristics, planting date, plant height, growth shape, leaf length, leaf shape, petiole color, petiole, stem color, flower color, after harvest, Ml length including roots, flower length, stem thickness and biological weight were measured. For anthocyanin analysis, only basil with purple color was selected, anthocyanins were extracted with 60% ethanol containing 1% citric acid, a standard quantitative curve was prepared with cyanidin-3-glucoside, and absorbance was measured at 525 nm. Basil planting started around June 16, and the flowering period lasted for a total of 18 days from July 19 to August 5. On August 6, when flowering was completed, morphological characteristics including flower color were measured at the site by an object. The measured basil plant length was 26.6-59.6 cm, leaf length 2.0-7.9 cm, leaf width 0.7-5.2 cm, and petiole 1.3-3.9 cm. The growth pattern of basil was generally straight, medium in shape. The color of the leaves was observed evenly by mixed green, purple, purple and green, and the recesses in the shape of the leaf were also conspicuously observed. A total of three basil flower colors were observed: white, light pink(149P 10P/84), and purple(37V 2.5RP 4/12). During the second growth survey after harvest, the total length including the basil roots is 26.7-52.0 cm, the flower length is 8.2-29.3cm, the stem thickness is 7.1-15.9mm, the number of stem nodes is 3-12 nodes, and the total weight of the basil was measured to be about 218-1540 g. There are a total of 16 types of basil expressed in purple, and the anthocyanin content was measured to be 73.74 ~ 380.72 mg/100g.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.25-25
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• 2020

산초나무(Zanthoxylum schinifolium Siebold & Zucc.)는 운향과(Rutaceae)에 속하는 낙엽활엽관목으로 주로 열매, 잎, 줄기는 식·약용으로 이용한다. 최근 산초나무 부위별 추출물에서 항산화활성, 항염증효과, 미백효과 등에 관한 연구가 이루어지면서 산초나무의 가치가 더욱 증가하고 있다. 본 연구는 산초나무의 효율적인 유·무성 증식법을 구명하기 위해 종자 처리에 따른 발아특성, 생장조절물질 처리에 따른 가지삽목과 뿌리삽목의 발근특성을 조사하였다. 산초나무 종자는 3개월간 저온습사저장(종자:모래=1:1, -4℃)을 실시할 경우 약 64.0%가 발아하였다. 습사저장된 종자를 파종 직전에 GA3를 이용하여 0, 500, 1,000 및 2,000ppm 농도에서 처리한 결과 각 처리구별 발아율은 유의한 차이를 나타내지 않았다(F=1.556, p=0.260). 다만, 500ppm 농도에서 72.0%가 발아하여 가장 높은 발아율을 보였으며 1,000ppm 이상 처리구에서는 발아율이 다소 감소하는 경향을 나타냈다. 무성증식 방법은 2월 중순에 산초나무 가지삽수와 뿌리삽수를 채취한 후 IBA와 NAA를 각각 0, 500, 1,000 및 2,000ppm 농도로 처리하였다. 가지삽목을 실시한 대부분의 삽수에서 신초가 발생했으나, 발근율은 1.3~6.3%로 현저히 낮았다. 반면, 뿌리삽목은 3개월 후 부정아 발생율이 처리에 따라 58.3~83.3%로 조사되었으며, 발근율은 0~66.7%를 나타나 처리구별 발근율에 유의한 차이가 있었다(F=3.744, p=0.020). IBA 처리가 근삽수의 발근에는 효과적이었으며, 특히 IBA 2,000ppm 처리에서 66.7%의 발근율을 보여 가장 효과적임을 알 수 있었다. 결론적으로, 산초나무의 효율적인 유성증식을 위해서는 종자를 3개월간 저온습사저장하는 것이 효과적이며, 무성증식 방법으로는 가지삽목보다 뿌리삽목을 이용하는 것이 더 효과적인 것으로 판단된다.

• Journal of Forest and Environmental Science
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• v.19 no.1
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• pp.85-98
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• 2003

The articles, published in the Journal of Korean Forestry from Number 1(1962) to Number 6, Volume 91(2002), were surveyed and investigated for the research trend analysis about resource-plants for special use or purpose, i. e., edible plants, medicinal plants, feed resource, landscape plants, fiber plants, industrial usuage, and bee plants. If the purpose or subject matter of the research was construction or furniture timber production, mushrooms and/or pulp and paper, such research was not included in this study. These articles were classified again depending on the content of research into 14 categories: habitat environment, ecology, physiology, propagation, silviculture (tending and culture), genetics and breeding, identification, insect and disease control, animal-related research, component analysis, vegetation survey, biotechnology, management, and review. Among the total 1.434 articles published, 396 ones (27.6%) were related with plants for special use or purpose. Vegetation survey was 60 (15.2%): physiology 56(14.1%) : genetics and breeding 56(14.1%): propagation 53(13.4%): and ecology 37(9.3%). Siviculture research field included 11 articles (2.8%), which indicates that the management of resource-plants is so far from economic income as seen in the low number of management research filed articles, i. e., only 6 reports (1.5%) Korean white pine was most popular for research and included 42 articles: Robinia pseudoacacia 23: Castanea crenata 14: and ginkgo tree 14. Research related with these species had focused mainly on propagation, physiology, genetics and breeding, ecology and pest control. Based on this survey and analysis, the followings are suggested: 1. More research is required on forest herbaceous plants. 2. Cooperative research work with other industrial and/or scientific area is recommendable for commercialization including medicine, cosmetics, and food etc. 3. Research on resource-plant conservation, which includes biology, social education and policy, should be supported for next generation. 4. Mutual correspondence and information exchange about the research results between researchers and institutes is more necessary than now.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.511-517
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• 2015

This research was conducted to evaluate the influence of root restriction materials and media on the growth of runner plantlets of 'Seolhyang' strawberry in a nursery field. To achieve this, the influence of three kinds of root media on the growth of runner plantlets was monitored when polyethylene film was used as the root restriction material. In addition, the influence of various root restriction materials (RRS) such as transparent polyethylene film (PE), non-woven fabric (NF), perforated polyethylene film (PP), and root proofing sheet (RPS) on the changes in volumetric water content (VWC) and temperature of root media as well as growth of runner plantlet were investigated when expanded rice hull (ERH) was used as the root medium. In the comparison of root media, growth parameters such as leaf area and crown thickness at 20 d after fixation as well as crown thickness and fresh weights of root and above-ground tissue at 40 d after runner plantlet fixation were higher in the ERH treatment than in sandy loam and loamy sand. When the influence of RRS was compared, the VWC of ERH was 55% just after irrigation, but decreased to 26% at just before irrigation. Ranges of the VWC as influenced by irrigation cycle were 16 to 10% in the PP and less than 10% in the NF and RPS. The soil temperature in the PE treatment was around $1^{\circ}C$ lower than in NF, PP, and RPS. The differences between day and night temperatures were also smaller in the PE treatment rather than those in NF, PP, and RPS. The growths of runner plantlet 50 d after fixation showed that plant heights as well as fresh weights of root and above-ground tissue were higher in the PE treatment than in NF, PP, and RPS. NF and PP did not effectively restrict roots inside the medium and the roots of runner plantlets penetrated through the root restriction materials resulting in the formation of root system below the restriction materials. The above results indicate that ERH is more effective than sandy loam or loamy sand as root medium. PE rather than NF, PP, or RPS as root restriction material resulted in better growth of runner plantlets in propagation of 'Seolhyang' strawberry. The results of this research will be used for production of high quality runner plantlets in strawberry propagation.

• Korean Journal of Plant Taxonomy
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• v.46 no.2
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• pp.256-266
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• 2016

A total of 13,000 individuals of Dendrobium moniliforme (L.) Sw. artificially propagated in laboratories and greenhouses were restored in their natural habitat of Bogildo Island, Wandogun, in the southern part of Korea in June of 2013. The growing conditions of the individuals were monitored for two years. The parental individuals for the restoration were obtained from a wild population in southern Korea, from which seeds were produced via artificial crossings. These seeds were germinated and cultivated in growing media and two-year-old plants were then grown in greenhouse beds. The genetic diversity among the propagated individuals was confirmed by examining DNA sequences of five regions of the chloroplast genome and the nuclear ITS region. The diversity values were as high as the average values of natural populations. All propagated individuals were transplanted into two different sites on Bogildo by research teams with local residents and national park rangers. After restoration, we counted and measured the surviving individuals, vegetative propagated stems, and growth rates in June of both 2014 and 2015. There was no human interference, and 97% of the individuals survived. The number of propagules increased by 227% in two years. In contrast, the average length of the stems decreased during the period. In addition, different survival and propagation rates were recorded depending on the host plants and the restored sites. The shaded sides of rock cliffs and the bark of Quercus salicina showed the best propagation rates, followed by the bark of Camellia japonica. A few individuals of D. moniliforme successfully flowered, pollinated, and fruited after restoration. Overall, our monitoring data over two years indicate that the restored individuals were well adapted and vigorously propagated at the restored sites. In order to prevent human disturbance of the restored sites, a CCTV monitoring system powered by a solar panel was installed after the restoration. In addition, a human surveillance system is operated by national park rangers with local residents.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.219-226
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• 2015

The objective of this research was to investigate the influence of various root media on the growth of mother and daughter plants during propagation and early yield after transplanting of 'Seolhyang' strawberry. To achieve this, daughter plants were fixed to connected small pots that contained expanded rice-hull (ERH), a strawberry-specialized commercial medium (SSCM), soil mother materials (SMM), or loamy sand (LS). Then, growth of daughter plants in above- and below-ground tissue as well as early yield after transplanting to plastic house soil were investigated. The growth of daughter plants in terms of plant height, leaf area and fresh weight were the highest in the SSCM treatment. Root growth in terms of the amount of primary roots and root dry weight were the highest in the treatments of ERH and SMM and the lowest in that of SSCM, among treatments tested. The ERH treatment also showed the highest values among treatments in root length, surface area and volume when roots with 0 to 0.4 mm in diameter were investigated. The flower bud differentiation of daughter plants began on Sept. 3 in the ERH treatments, earlier than the SMM (Sept. 5) and in SSCM (Sept. 7) treatments. The tissue N contents of daughter plants were in the range of 1.41 to 1.55% in all treatments, and no significant differences were observed among treatments. This indicates that the low moisture retention capacity of ERH and water stress, rather than tissue N contents, promote the flower differentiation of daughter plants. In the evaluation of early yield after transplant, the ERH treatment of showed the highest yield in the period from November to December, reaching 667 g fruit weight per 10 plants. The yields per 10 plants in the other treatments were 581 g in SMM, 475 g in SSCM and 295 g in LS. Above results imply that the various root media have different effects on the growth of daughter plants as well as flower bud differentiation. Therefore, improvement in early yield after transplant can be achieved through selection of proper root medium for daughter plant propagation.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.657-663
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• 2012

The Ca deficiency is a serious problem in the propagation of domestically bred 'Seolhyang' strawberry through soil cultivation. The objective of this research was to investigate the impact of the Ca containing fertilizers on the decrease of Ca deficiency symptoms and in the growth of mother and daughter plants. To achieve this, chemicals of 1.125 mM $Ca(OH)_2$, 0.375 mM $MgCl_2$, and 1.25 mM KCl were blended to contain the K:Ca:Mg (4:2:1) and the influence was compared to a commercial fertilizer, Azuro-Calma ($NO_3$-N 13%, $K_2O$ 1%, CaO 16%, MgO 6%), when those were applied as solutions with the electrical conductivity (EC) controlled to 0.6 or 1.0dS ${\cdot}m^{-1}$. During 120 days after transplant, the Azuro-Calma was more effective than the combined fertilizer in the reduction of mother and daughter plants on which Ca deficiency was appeared. The application of two Ca containing fertilizers resulted in the heavier fresh and dry weights of mother plants. The treatments of 1.0dS ${\cdot}m^{-1}$ of two fertilizers were more effective than those of 0.6dS ${\cdot}m^{-1}$. The combined fertilizer was also more effective than Azuro-calma on the growth of above ground plant tissue. The results in length, fresh and dry weight of runners occurred from a mother plants showed that the treatment of 1.0dS ${\cdot}m^{-1}$ of combined fertilizer was most effective followed by those of 1.0dS ${\cdot}m^{-1}$ of Azuro-Calma, 0.6dS ${\cdot}m^{-1}$ of combined fertilizer, 0.6dS ${\cdot}m^{-1}$ of Azuro-Calma, and control. The fresh weights of daughter plants were heavier in the treatments of Ca application than those in the control treatment, but the differences between 0.6dS ${\cdot}m^{-1}$ and control were not significant. The above results indicate that Azuro-Calma is more effective in decreasing plants showing the Ca deficiency symptoms. However, the combined fertilizer of K:Ca:Mg is more desirable when we are concerned about the decrease of crops showing Ca deficiency as well as increase of the growth in above ground plant tissue.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.337-351
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• 2015

The articles, published in the Journal of Korean Forest Society from Volume 1 (1962) to Volume 102 (2013), were investigated for the research trend analysis about forest plants for special purposes, i.e., edible plants, medicinal plants, feed resources, landscape plants, fiber plants, industrial usage, bee plants, bioenergy/phytoremediation uses, dye materials, and rare/endangered/endemic plants. These research articles were classified again based on the contents of research into following categories - habitat environment, ecology, physiology, propagation, silviculture (including planting and tending), genetics and breeding, identification, pest and disease control, animal-related research, components analysis and extracts, vegetation survey, biotechnology, management, recreation and forest healing, and research review. Among the total 2,433 articles published, 611 (25.1%) were related to plants for special usage or purposes. The highest frequency (14.9%) in publications was found in the field of silviculture followed by physiology, propagation, identification, and genetics and breeding, respectively. On the bases of usage, edible plants showed higher frequency (26.5%) than others, followed by industrial purpose, bioenergy/phytoremediation usage, landscape plants, medicinal plants, and rare/endangered/endemic plants. Populus plant species was the most popular in research, showing 62 articles; and Castanea crenata 36; Pinus koraiensis 35; Robinia pseudoacacia 20; Ginko biloba 17, etc. Based on the survey and analysis, the following points are suggested: 1) improved evaluation of forest plants as non-wood resources, 2) expanding research topics on the basis of production, management, and utilization of non-wood forest resources, 3) management of database of forest plant information and encouragement needed to strengthen cooperative researches satisfying the needs of other industrial and scientific areas, and 4) encouraging to promote traditional knowledge based research on forest plants.