• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.3
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• pp.1-12
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• 2000

식물생장조절제(Plant Growth Regulators, PGRs)를 이용한 예초관리 방법이 들잔디 엽색 및 품 질에 미치는 영향을 규명함으로 잔디 적응력 관점에서 적합한 PGR 종류 및 살포수준을 파 악하고자 본 연구를 시작하였다. 실험은 들잔디 생육이 왕성한 시기인 6월(실험 I)과 7월(실험 II) 2회에 걸쳐 각각 다른 장소에서 실시하였다. 공시약제 처리구는 Type I PGR인 amidochlor 3 수준 및 mefluidide 3 수준 과 Type II PGR인 trinexapacethyl 4 수준, 그리고 무처리구를 포함하여 전체 11 처리구였 고, 실험구 배 치는 난괴법 4 반복으로 하였다. 공시한 3종류 PGR 모두 잔디엽색 및 품질에 통계적으로 유의한 차이 가 있었지만, PGR 종류.살포수준 및 생육 상태에 따라 처리효과는 다르게 나타났다. 잔디 엽색 및 품질 은 PGR 살포후 경과기간에 따라 점진적으로 억제되는 경향이었다. 처리 1주후 잔디품질은 대조구에 비 해 큰 차이가 없었지만, 2주에서 4주까지는 PGR 종류 및 살포수준에 따라 유의하게 감소하 여 0.08 ml/ $m^2$ 이상의 trinexapac-ethyl 처리구 및 1.20 ml/$m^2$ 이상의 mefluidide 처리구에서는 가장 크 게 나타났다. 처리 4주부터 대부분 처리구에서 잔디품질이 적정수준 이상으로 회복되기 시작하였다. 본 실험을 통해 잔디관리 수준에서 저관리 잔디지역은 0.08 ml/$m^2$ 수준의 trinexapac-ethyl 처리구가 장기간 생장억제 관점에서 실용성과 경제성 측면에서 들잔디 관리에 효율적인 것으로 판단되었고, 고관리 잔 디지역은 0.04~0.08ml/$m^2$ 수준의 trinexapac-ethyl 처리구 및 0.60~1.20 ml/$m^2$ 수준의 amidochlor 처 리구가 적합 한 것으로 판단되었지만, 계절에 따라 PGR 효과정도가 다르게 나타났다. 따라서, 잔디 관리 시 잔디의 생육상태, 예초 절감 기간, 잔디품질의 기대수준 및 적용할 수 있는 관리수준에 따라 적합한 PGR 종류 선정과 함께 살포시기 및 살포량을 효율적으로 결정하는 것이 필요한 것으로 사료되었다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.225-229
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• 1999

Unstable seedling stand establishment of wet direct seeding culture of rice is one of the major elements preventing the extension of its culture area. In order to develop methods of seedling stand improvement in direct seeded rice on flooded surfaces, three field experiments were conducted on silty loam soil using a cultivar 'Donjinbyeo' for three years, mainly focusing on water management after seeding and seed soaking with plant growth regulators (PGRs). Under the condition of shallow flooding after seeding, seedling stand rate increased and floating seedling rate decreased in both early and normal season seeding compared to deep flooding. With earlier draining time after seeding, there was a tendency towards preferential growth of the seminal root, increase of seedling stand and decrease of the floating seedling rate. Therefore the highest seedling numbers per unit area and the lowest floating seedling numbers were found upon drainage at 1 day after seeding (DAS), while a contrary tendency was shown upon conventional drainage at 7 DAS. Seed soaking with PGRs such as Metalaxyl or mixing of Metalaxyl with gibberellic acid (GA$_3$) significantly increased the seedling stand. In addition the effects of PGR treatment on seedling stand and the early growth of plants were greater under flooded conditions than under drained conditions after seeding, although draining of water after seeding improved the seedling establishment rate more when compared with the PGR treatment. These results suggest that draining management after seeding or maintaining of shallow flooding for a week is the most effective method to improve the seedling stand rate in wet direct seeding.

• Current Research on Agriculture and Life Sciences
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• v.32 no.4
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• pp.179-183
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• 2014

Optimization of the protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids was performed by determining the effects of plant growth regulators (PGRs) and different parts and division sizes of the PLB. For both genera, the base part was the best for the proliferation of PLBs, yielding the highest number of PLBs on a PGR-free medium for Phalaenopsis and medium containing $0.1{\mu}M$ NAA and $10.0{\mu}M$ BAP for Dendrobium. As regards the division size, four-division sections resulted in a higher PLB formation efficiency for Phalaenopsis, while two-division sections produced a higher PLB formation efficiency for Dendrobium. It is expected that these findings will be applicable to efficient PLB formation of other Phalaenopsis and Dendrobium orchids.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.1
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• pp.101-109
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• 2016

This is the fundamental research on restoring damaged vegetation areas in the vicinity of DMZ using local native plant species. This research is aimed at identifying effective plant growth regulators (PGR) for seed germination and seedling growth of mountain mulberry, and developing effective methods for managing its germination and growth. Mountain mulberry seeds were collected from the regions in the DMZ vicinity, and tests with seven treatments using four PGRs including $GA_3$, kinetin, ABA and NAA, were conducted. The germination rate was calculated in two different ways of both in a growth chamber and in a greenhouse after seven days observation, and the growth characters such as leaf width/length, seedling width/length and fresh/dry weight, have been surveyed in a greenhouse for three months. Although in the growth chamber the highest germination rate was shown at a group with the kinetin treatment, it was not significantly different to that of the control group. Groups with ABA or NAA presented relatively low germination rates. As for the greenhouse test, the germination rates of all groups ranged 20~30% without significant difference each other, the reason of which might be due to low absorption by the effect of a soil drench method used in this study. The entire growth characters with the treatments of $2.15mg{\cdot}L^{-1}$ of kinetin and $10mg{\cdot}L^{-1}$ of $GA_3$ were significantly different to the control. NAA treatment only showed better growth of seedling width compared to the control. Consequently, the most effective PGRs for the germination and growth of mountain mulberry near the DMZ was kinetin. Further research on examining the most effective concentration of them was needed.

• Korean Journal of Medicinal Crop Science
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• v.14 no.5
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• pp.278-281
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• 2006

The effect of plant growth regulators was investigated on in vitro shoot proliferation from axillary bud explants of Hypericum erectum. To determine the optimal cytokinin for proliferation of axillay buds, we carried out screening four cytokinins (BA, kinetin, 2iP, TDZ). When nodal segments were cultured on MS medium supplemented with $4.5\;{\mu}M$ TDZ (thidiazuron), a number of shoots were induced. Our results indicated that the addition of TDZ to culture medium resulted in the induction of significantly more axillary buds than in the addition of other cytokinins. The optimal concentration of TDZ for proliferation of axillary buds was $10\;{\mu}M$. 92% of shoots spontaneously rooted without any plant growth regulator (PGR) and formed whole plantlets within one month. More than 95% of these regenerants survived and they did not show any detectable variation in morphology or growth characteristics compared to their donor plants.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.51-59
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• 2008

Lettuce, a typical light-induced seed germination type, exhibits different germination responses according to cultivars, light quality, and plant growth regulator (PGR) treatments. Germination rates in most tested cultivars were over 85% under both white and red light, and were slightly decreased by blue light. Although photo-inhibition in germination was observed from most cultivars by far-red light, 'Cheongguangcheongchima', 'Okdol', and 'Manchudaecheongchima' could be classified as photo-insensitive lettuce cultivars by exhibiting the germination rates as 78,63, and 48% under for-red light, respectively. 6-Benzylamino purine (BAP) and kinetin promoted seed germination and normal seedling production under far-red light, but ethephon did not show any positive effects. Cytokinins such as BAP, kinetin, thidiazuron (TDZ), and zeatin overcame photo-inhibition of seed germination even the concentration of below $50\;mg{\cdot}L^{-1}$. However, auxins such as IBA, 2,4-D, and NAA failed to overcome the far-red light-induced photo-inhibition.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.62-62
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• 2022

Freesia has been an important worldwide cut flower because of its fragrance, long vase life and the wide color range of the flower. The conventional propagation methods by seeds and corms have many disadvantages such as shorter inflorescences with fewer numbers of florets, a reduction in cut flower quality and the accumulation of plant viruses in corms by successive cultivation. Therefore, the conventional propagation systems in Freesia needs to be replaced with tissue cultures to overcome the disadvantages. This study explored an efficient multiplication protocol using the combination of plant growth regulators (PGRs) for developed cultivar 'Sunny Gold'. The combination between 6-benzylaminopurin (BA) and α-naphthalene acetic acid (NAA) did not produce new shoots but developed enlarged roots. BA only treatments and the combination between BA and kinetin treatments were effective on shoot multiplication. The highest average number of shoots was 5.3 in the presence of 3 mg/L BA and 0.5 mg/L kinetin. To produce corms and cormlets, proliferated shoots were subcultured on 1/2 Murashige and Skoog (MS) medium supplemented with 90 g/L sucrose, 1 g/L charcoal and 7 g/L plant agar and placed at 4℃ in the dark for 6 months. The small size of corms and comlets were produced. The average number of regenerated comlets was 2.75 per shoot. The results showed that shoot multiplication is more efficient than cormlet regeneration for in vitro freesia proliferation.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.261-265
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• 2010

This experiment was carried out in order to determine proper plant growth regulators (PGR) and their concentrations for direct shoot induction and somatic embryogenesis from leaf tissue cultures of Muscari armeniacum 'Early Giant'. Direct shoot formation from the leaf explant culture was effective only on a half-strength MS medium containing $0.1mg{\cdot}L^{-1}$ 2,4-D, while embryogenesis was occurred on a half-strength MS medium containing $0.1{\sim}1.0mg{\cdot}L^{-1}$ IPA or without PGR. The regenerated bulblets derived from embryos or shoots were harvested and transplanted into a greenhouse. The sprouting percentage of bulblets obtained from different culture media ranged from 80 to 100% and growth of quality bulblets was enhanced when the bulblets were harvested from the medium containing $0.1mg{\cdot}L^{-1}$ NAA and $1.0{\sim}3.0mg{\cdot}L^{-1}$ IPA.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.325-332
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• 2017

This research was conducted to evaluate methods of enhancing the waterlogging resistance of soybean plant. Thus, we applied seven types of plant growth regulators (PGRs) to soybean plants and exposed them to waterlogged conditions for a total of 14 days. To evaluate stress resistance, we monitored plant growth characteristics data such as height, chlorophyll content, and chlorophyll fluorescence for 28 days after the initial waterlogging (14 days under waterlogging conditions and 14 days after waterlogging). According to the results, plant height was significantly increased by gibberellin A4 ($GA_4$) treatment compared to the control treatment and waterlogging-only treatment. However, we could not detect plant height owing to plant death when we applied abscisic acid (ABA). Except for $GA_4$ and ABA treatments, plant heights slightly decreased in all treatments compared to the waterlogging-only treatment. The chlorophyll content and chlorophyll fluorescence showed a similar tendency among PGR treatments. The chlorophyll content and chlorophyll fluorescence were significantly increased by ethephon and kinetin treatments 28 days after waterlogging compared to the waterlogging-only treatment. Consequently, kinetin and ethephon treatments induced more resistant phenotypes in soybean plants during or after exposure to waterlogging conditions.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.133-141
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• 2022

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L^-1 of BAP and 2.0 mg·L^-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L^-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L^-1 of banana and 20 g·L^-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L^-1 of coconut water) and plant growth regulators (2.0 mg·L^-1 of BAP and 1.0 mg·L^-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.