• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.150-156
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• 2020

Weeds play an important role in the survival of viruses and are potential inoculum sources of viral diseases for crop plants. In this study, specimens of Pseudostellaria heterophylla exhibiting symptoms of the cucumber mosaic virus (CMV) were collected in Bonghwa, Korea. The characteristics of the disease were described and leaf RNA was extracted and sequenced to identify the virus. Three CMV contigs were obtained and PCR was performed using specific primer pairs. RNA from positive samples exhibiting CMV leaf symptoms was amplified to determine the coat protein. A sequence comparison of the coat protein gene from the CMV BH isolate shared the highest nucleotide identity (99.2%) with the CMV ZM isolate. Phylogenetic analysis showed that CMV-BH belonged to subgroup IA and that the most closely-related isolate was CMV-ZM. All test plants used for the biological assay were successfully infected with CMV and exhibited CMV disease symptoms such as blistering, mosaic, and vein yellowing. To our knowledge, this is the first report of CMV infection in P. heterophylla from Korea.

• Journal of Korean Society on Water Environment
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• v.35 no.4
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• pp.370-378
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• 2019

In the recent years, various studies have presented stable and economic methods for increased regulations and compliance in sewage treatment plants. In some sewage treatment plants, the effluent concentration exceeded the regulations, or the effluent concentration was manipulated. This indicates that the process is currently inefficient to operate and control sewage treatment plants. The operation and control method of sewage treatment plant is mathematically dealing with a physical and chemical mechanism for the anticipated situation during operation. In addition, there are some limitations, such as situations that are different from the actual sewage treatment plant. Therefore, it is necessary to find a more stable and economical way to enhance the operational and control method. AI (Artificial Intelligence) technology is selected among various methods. There are very few cases of applying and utilizing AI technology in domestic sewage treatment plants. In addition, it failed to define specific definitions of applying AI technologies. The purpose of this study is to present the application of AI technology to domestic sewage treatment plants by comparing and analyzing various cases. This study presented the AI technology algorithm system, verification method, data collection, energy and operating costs as methods of applying AI technology.

• Plant Breeding and Biotechnology
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• v.5 no.3
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• pp.213-220
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• 2017

Production of spring wheat, the major crop in Mongolia, accounts for 98% of the cultivated area. Understanding genetic variability in existing gene bank accessions is critical for collection, conservation and use of wheat germplasms. To determine genetic diversity and population structure among a representative collection of Mongolian local wheat cultivars and lines, 200 wheat accessions were analyzed with 15 SSR markers distributed throughout the wheat genome. A total of 85 alleles were detected, with three to five alleles per locus and a mean genetic richness of 5.66. Average genetic diversity index was 0.69, with values ranging from 0.37-0.80. The 200 Mongolian wheat accessions were mainly divided into two subgroups based on structure and phylogenetic analyses, and some phenotypes were divergent by the subgroups. Results from this study will provide valuable information for conservation and sustainable use of Mongolian wheat genetic resources.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.102-108
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• 2000

Morphological characteristics of nine Epimedium spp was analyzed on the basis of six morphological characters. To analyse the genetic relationship among Epimedium spp., polymerase chain reaction (PCR) was performed with total genomic DNA of 17 Epimedium spp. by using random 8 primers. The genetic diversity and genetic distance among nine Korean collections, seven Japanese and one China collection were used to generate a dendrogram showing phylogenie relationship. Seventeen Epimedium spp were classified into two groups of group I and II, since they were divided into two major groups at the similarity coefficients value of 0.65. In addition, one of the two group, group I was divided into three sub-groups including Epimedium koreanum, Chul-won collections 1, 2, and 3, Yanggu, Hongchon, Hwachun, Chunchon, China, Maehwa (Japanese), E. diphyllum and E. violaceum (Japanese), while group II included Chulwon collection 4 and 5 and Japanese collection. The samples collected at Chulwon district showed close similarity with Japanese collection. Similarity indexes between collection and genetic relationship were related at the levels ranging from 0.6 to 0.9.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.4
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• pp.445-449
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• 2005

Present study was designed to evaluate the practical application of triethyl amine(TEA) injection for improving the collection efficiency of electrostatic precipitator(ESP) connected to a real operating plant. The major fuels used at the domestic power stations were bituminous coals imported from Australia, China, South Africa, and USA. Although the values of the electric resistance would be more or less different according to the type of the coals used, the unique electric resistance values of fly ash from the coals were mostly higher than $1{\times}10^{12}\;{\Omega}-cm$ and therefore, back corona problems were always expected to occur in the electrostatic precipitator. The particulates concentrations, smoke concentrations and their electric resistivity measured at the outlet of ESP, and the inspection of collection indicated that the injection of TEA improved the collection efficiency of particulate at collection plates of ESP. The electric resistance for, fly ash with the injection concentration of TEA 15 ppm(Purity 99.7%) was lowered to $2.1{\times}10^{11}\;{\Omega}-cm$ after injection from $1.9{\times}10^{12}\;{\Omega}-cm$ before injection. Under this condition, the dust emission content at the stack was reduced to approximately 80%, lowering the average outlet concentrations of particulates from $70\;mg/Sm^3$ to $14\;mg/Sm^3$.

• The KSFM Journal of Fluid Machinery
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• v.18 no.5
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• pp.26-32
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• 2015

This paper describes performance evaluation of design parameters, air velocity inside a pipeline and pressure along a pipeline, using experimental measurements in an automated vacuum waste collection system. Automatic robot having six cameras is introduced to analyze the internal pipeline conditions whether waste accumulates at the bottom of the pipeline or not. Throughout the experimental measurements of the pipeline having the various shapes, it is found that pressure and internal air velocity linearly increase along the pipeline from a waste inlet to a waste collection station while air density decreases due to the air compression effect with high pressure. Although air velocity inside the pipeline at a waste inlet keeps design velocity range between 20 m/s and 30 m/s, it is noted that air velocity near the waste collection station exceeds maximum design velocity of 30 m/s. Pressure increase per unit length is changed from 17.6 Pa/m to 18.9 Pa/m, which depends on the air velocity inside the pipeline. From the investigation inside the pipeline with CCTV loaded on an automated robot, waste accumulated at the bottom of the pipeline is mainly found at the downstream of a circular curved pipe, an inclined pipe and a bended pipe.

• Journal of Microbiology and Biotechnology
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• v.29 no.5
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• pp.794-808
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• 2019

Bacillus velezensis strain WRN014 was isolated from banana fields in Hainan, China. Bacillus velezensis is an important member of the plant growth-promoting rhizobacteria (PGPR) which can enhance plant growth and control soil-borne disease. The complete genome of Bacillus velezensis WRN014 was sequenced by combining Illumina Hiseq 2500 system and Pacific Biosciences SMRT high-throughput sequencing technologies. Then, the genome of Bacillus velezensis WRN014, together with 45 other completed genome sequences of the Bacillus velezensis strains, were comparatively studied. The genome of Bacillus velezensis WRN014 was 4,063,541bp in length and contained 4,062 coding sequences, 9 genomic islands and 13 gene clusters. The results of comparative genomic analysis provide evidence that (i) The 46 Bacillus velezensis strains formed 2 obviously closely related clades in phylogenetic trees. (ii) The pangenome in this study is open and is increasing with the addition of new sequenced genomes. (iii) Analysis of single nucleotide polymorphisms (SNPs) revealed local diversification of the 46 Bacillus velezensis genomes. Surprisingly, SNPs were not evenly distributed throughout the whole genome. (iv) Analysis of gene clusters revealed that rich gene clusters spread over Bacillus velezensis strains and some gene clusters are conserved in different strains. This study reveals that the strain WRN014 and other Bacillus velezensis strains have potential to be used as PGPR and biopesticide.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.257-262
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• 2021

The pinning method is the traditional method to produce strawberry transplants. But, cultivating the pinning transplant is a labor-intensive operation and needs a long labor period. The cutting method has been considered as an effective alternative to the pinning method, due to the relatively short labor period and works time. This study was conducted to investigate the labor period, work time, and growth between pinning and cutting methods for strawberry transplants. The 'Maehyang' strawberry was cultivated at each pinning and cutting strawberry greenhouses. The time for special works on pinning method (pinning work, elimination of mother plant, and division of daughter plant), and cutting method (cutting collection, pretreatment before storage, and cutting work) were measured. The pinning method needed 6 tasks (planting of mother plant, maintaining of mother plant, pinning work, maintaining of daughter plant, elimination of mother plant, and division of daughter plant) for 158 days, and cutting method needed 4 tasks (collection and storage of cutting, cutting work, misting, and maintaining of transplants) for 113 days to cultivate transplants for fruit. And pinning method needed more work time than the cutting method. There was no significant difference between the growth of pinning and cutting transplants. These results showed that the cutting method saved more labor period, work time than pinning during the nursery period without losing transplant quality.

• IE interfaces
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• v.20 no.1
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• pp.58-68
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• 2007

This research deals with a vehicle scheduling problem for the sludge collection strategies which might be solved via quantitative analysis and cost evaluations schemes. This problem can be modeled as a kind of capacitated vehicle routing pick-up problems. With the aim of establishing operation schedule of vehicles and analyzing the total cost under considering various assumptions and realistic restrictions of the sludge collection problem, we propose a heuristic method based on the genetic algorithm in conjunction with the sweep algorithm and the 4-opt algorithm. Finally, we present the cost effective operation schedule that can be used as the managing tool for the sludge treatment plant of the multi-purpose dam.

• The Plant Pathology Journal
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• v.23 no.2
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• pp.37-44
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• 2007

Colletotrichum contains many important pathogens which cause economically significant diseases of crops like pepper, strawberry, tomato and apple. Forty four isolates were collected to characterize the diversity of Colletotrichum causing apple anthracnose in various regions of Korea. They were analyzed by random amplified polymorphic DNA (RAPD), internal transcribed spacer (ITS) of rDNA and partial $\beta$-tubulin gene DNA sequence, and culture characteristics on PDA and PDA-Benomyl. From the results of molecular analyses, 31 strains belonged to Colletotrichum gloeosporioides, ribosomal DNA group (RG) 4 of Moriwaki et al. (2002), 8 strains belonged to C. acutatum, A2 group of Talhinhas et al. (2005) and 5 strains to C. acutatum, A3 group of Talhinhas et al. (2005). Most isolates of C. gloeosporioides RG4 grew faster on PDA than strains of C. acutatum, A2 and A3 groups and most RG4 strains were sensitive to Benomyl. However, a few strains of RG4 grew slower and were resistant to Benomyl. On the basis of molecular characteristics, apple isolates of C. acutatum were clearly differentiated from red pepper isolates of the species, but apple isolates of C. gloeosporioides were not.