• KSBB Journal
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• 제13권3호
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• pp.259-267
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• 1998

Optimal feeding strategies in bioreactor operation of Scutellaria baicalensis G. plant cell culture were investigated to maximize the production of flavone glycosides by using a structured kinetic model which can predict culture growth and flavone glycosides synthesis in a rigorous, quantitative manner. For the production of baicalin and wogonin-7-0-GA, the strategies for glucose feeding into Scutellaria baicalensis G. plant cell culture were proposed based on the model, which are a periodic fed-batch operation with maintenance of cell viability and of specific production rate respectively, and a perfusion operation with maintenance of specific production rate for baicalin and wogonin-7-0-GA. Simulation results showed that the highest volumetric concentration of flavone glycosides was obtained in a periodic fed-batch operation with maintenance of cell viability among all the suggested strategies. In the periodic fed-batch operations, the higher volumetric production of flavone glycosides was achieved compared with that in the perfusion operation. It can be concluded that a periodic fed-batch operation with maintenance of cell viability would be the optimal and practical operating strategy of Scutellaris baicalensis G. plant cell culture for the production of flavone glycosides.

• Molecules and Cells
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• 제27권6호
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• pp.615-620
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• 2009

The plant shoot meristem maintains a group of stem cells that remain active throughout the plant life. They continuously generate new cells that are then recruited for organ initiation in the peripheral zone. Stem cell proliferation and daughter cell differentiation has to be integrated with overall growth and development of the diverse functional domains within the shoot apex. Several studies have revealed extensive communication between these domains. The signaling mechanisms employed comprise diffusible peptides, directional transport of plant hormones, but also complex interactions between transcription factors, that together establish a panoply of regulatory inputs that fine-tune stem cell behavior in the shoot meristem.

• 식물조직배양학회지
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• 제25권2호
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• pp.81-88
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• 1998

Southeast Asian javanica 벼 품종 Tinawen의 진탕 배양세포로부터 나출된 원형질체의 효과적인 배양과 식물체 재분화가 조사되었다. Lolium multiforum과 Oryza ridleyi의 진탕 배양세포들을 feeder cell로 사용했고 여러가지 재분화 배지를 이용하여 원형질체로부터 유도된 colony들을 재분화 시켰으며, 또한 식물체 재분화율을 높히기 위해 원형질체로 부터 유도된 colony들을 dehydration 시켜 재분화율을 조사하였다. L multiflorum 또는 O. ridleyi의 진탕 배양세포들을 feeder cell로 사용했을 때 원형질체의 평판효율은 feeder cell type과 age에 따라 차이가 났지만 0.09%에서 1.48% 범위로 나타났고, L. multiflorum을 feeder cell로 사용했을 때가 O. ridleyi cell을 사용했을때 보다 6배 높게 원형질체 평판효율을 얻었다. Feeder cell로 L. multiflorum을 사용하여 배양된 원형질채로부터 유도된 colony들을 dehydration 시킨 경우는 19.3-31.7%, O. ridleyi을 사용한 경우는 13.0-18.0%, 또한 이들 두 진탕 배양세포들을 혼합한 것을 사용한 경우는 18.0-22.0%의 식물체 재분화율을 얻은 반면에, dehydration을 시키지 않았을 때는 각각 2.0-7.0%, 3.0-5.0%, 0-4.0%의 재분화율을 얻었다. 원형 질체에서 재분화된 식물체의 flow cytometry를 이용한 배수성 분석 결과 대부분의 식물체가 이배체로 나타난 반면, 단지 34개중 두 식물체에서만이 4배체로 나타났다. 재분화된 식물체들은 온실에 옮겨 기른 결과 정상적인 임성을 나타내었다.

• Journal of Plant Biotechnology
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• 제34권2호
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• pp.129-137
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• 2007

Intercellular signaling is a crucial biological process for the coordination of cell differentiation, organ development and whole plant physiology. The intercellular movement of macromolecule signals such as proteins and RNAs has emerged as a novel mechanism of cell-to-cell communication in plant. Plasmodesmata, which are intercellular symplasmic channels, provide a key pathway for cell-to-cell trafficking of regulatory proteins / RNAs. This review specifically focuses on integrating the recent understanding on non-cell autonomous macromolecules, their function and regulatory mechanisms of intercellular trafficking through plasmodesmata.

• 한국자원식물학회지
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• 제30권3호
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• pp.265-271
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• 2017

In this study, we elucidated the molecular mechanism of silymarin by which silymarin may inhibits cell proliferation in human colorectal cancer cells in order to search the new potential anti-cancer target associated with the cell growth arrest. Silymarin reduced the level of c-Myc protein but not mRNA level indicating that silymarin-mediated downregulation of c-Myc may result from the proteasomal degradation. In the confirmation of silymarin-mediated c-Myc degradation, MG132 as a proteasome inhibitor attenuated c-Myc degradation by silymarin. In addition, silymarin phosphorylated the threonine-58 (Thr58) of c-Myc and the point mutation of Thr58 to alanine blocked its degradation by silymarin, which indicates that Thr58 phosphorylation may be an important modification for silymarin-mediated c-Myc degradation. We observed that the inhibition of ERK1/2, p38 and $GSK3{\beta}$ blocked the Thr58 phosphorylation and subsequent c-Myc degradation by silymarin. Finally, the point mutation of Thr58 to alanine attenuated silymarin-mediated inhibition of the cell growth. The results suggest that silymarin induces the cell growth arrest through c-Myc proteasomal degradation via ERK1/2, p38 and $GSK3{\beta}-dependent$ Thr58 phosphorylation.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.221-224
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• 2003

Leaf explants of Ixeris sonchifolia produced adventitious shoots at a frequency of 100% when cultured on MS medium supplemented with combinations of various concentrations of 6-benzyladenine (BA) (0.44, 4.44, or 8.87 ${\mu}M$) and 0.54 ${\mu}M$ NAA, or MS medium supplemented with 22.19 ${\mu}M$ BA and 2.69 ${\mu}M\;\alpha$-naphthaleneacetic acid (NAA) after four weeks of culture. Each explants (approximately $3{\times}6mm$) produced greater than 70 shoots at a combination of 0.44 ${\mu}M$ BA and 0.54 ${\mu}M$ NAA. Leaf explants produced shoots at a frequency of greater than 80% even at as low as 0.13 ${\mu}M$ BA as the sole growth regulator. Upon transfer to one-third strength MS with 0.54 ${\mu}M$ NAA, excised adventitious shoots were rooted at a frequency of 100%. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse. The competence of I. sonchifolia for plant regeneration via organogenesis appears to be greater than the competence of tobacco, currently the best model plant for organogenesis.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.112-119
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• 2005

Efficient plant regenerationsystem from cell suspension cultures was established in D. acicularis (2n = 90) by monitoring ploidy level and visual selection of the cultures. The highly regenerable cell lines selected maintained original ploidy level and consisted of compact cell clumps with yellowish color and relatively moderate growth, suggesting that it is possible to select visually the highly regenerable cell lines with the original ploidy level. All the regenerated plantlets from the highly regenerable cell cultures exhibited normal phenotypes and no variations in ploidy level were observed by flow cytometry (FCM) analysis.

• The Plant Pathology Journal
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• 제18권3호
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• pp.115-120
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• 2002

Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule of soybean, induces hypersensitive response (HR) in a non-host plant, hot pepper (Capsicum annuum). A wild-type strain (8ra) and its non-patho-genic mutant (8-13) of X. axonopodis pv. glycines were inoculated into the pepper leaf tissues and their subcellular responses to the bacterial infections were examined by electron microscopy. Intrastructural changes related to HR were found in the leaf tissues infected with 8ra from 8 h after inoculation, characterized by separation of plasmalemma from the cell wall, formation of small vacuoles and vesicles, formation of cell wall apposition, and cellular necrosis. No such responses were observed in the tissues infected with the mutant. In 8ra, the bacterial cells were attached to the cell walls, with the cell wall material dissolved into and appearing to encapsulate the bacterial cells. The bacterial cells later became entirely embedded in the cell wall material. On the other hand, in 8-13, the bacterial cells were usually not attached tightly to the plant cell wall, and no or poor encapsulation of the bacteria by the wall material occurred, although these were encircled by rather loose wall materials at the later stages.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.190-190
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• 2003

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.189-189
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• 2003