한국식물생명공학회:학술대회논문집 (Proceedings of the Korean Society of Plant Biotechnology Conference)
- 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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- Pages.112-119
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- 2005
Establishment of the cell lines with plant regeneration ability and low ploidy level in Dianthus acicularis with the aid of flow cytometry analysis
- Shiba, Tomonori (Faculty of Horticulture, Chiba University) ;
- Mii, Masahiro (Faculty of Horticulture, Chiba University)
- 발행 : 2005.11.02
초록
Efficient plant regenerationsystem from cell suspension cultures was established in D. acicularis (2n = 90) by monitoring ploidy level and visual selection of the cultures. The highly regenerable cell lines selected maintained original ploidy level and consisted of compact cell clumps with yellowish color and relatively moderate growth, suggesting that it is possible to select visually the highly regenerable cell lines with the original ploidy level. All the regenerated plantlets from the highly regenerable cell cultures exhibited normal phenotypes and no variations in ploidy level were observed by flow cytometry (FCM) analysis.
키워드