• Analytical Science and Technology
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• v.37 no.3
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• pp.155-165
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• 2024

This study aimed to assess whether plant-based meat substitutes can effectively replace animal meat products in terms of amino acid composition and zinc bioavailability. The evaluation was conducted in response to the growing demand for meat substitutes, driven by the increasing vegan population and the expansion of vegan culture. For this purpose, a chicken product and two plant-based meat substitutes in tender form were selected. The amino acid content and composition were measured using HPLC, while the levels of trace elements like zinc and calcium were determined through ICP-AES. Additionally, the presence of phytic acid, which inhibits zinc bioavailability, was extracted and quantified using UV-Vis spectroscopy. The results were analyzed in the context of daily product consumption. The findings revealed that certain essential amino acids, such as valine and lysine, were found to be deficient in plant-based meat substitutes compared to animal meat products. It was challenging to meet the recommended daily intake of these amino acids solely through the use of meat substitutes. Regarding zinc bioavailability, the inhibitory effect of calcium on zinc bioavailability was expected to be minimal. The zinc bioavailability of the meat substitutes varied significantly depending on the zinc and phytic acid content of the ingredients. Therefore, ingredients of plant-based meat substitutes should be carefully modulated to reach appropriate zinc bioavailability by selecting and processing plant materials with high zinc and low phytic acid content.

• Journal of Nutrition and Health
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• v.27 no.5
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• pp.451-459
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• 1994

This study was performed to investigate the effect of sources of protein on iron bioavailability in 10 healthy young Korean women. The 18-day metabolic study consisted of a 6-day adaptation period, 6-day moderate protein(60g protein/day, 18mg Fe/day) and 6-day high protein period(90g protein/day, 18mg Fe/day). During the moderate and high protein period, 5 subjects were fed the high plant protein meals(80% plant protein). Fecal excretion of dietary iron was significantly higher(p<0.05) in high protein high plant diet group(HPP, 9.48$\pm$1.61mg/day) than in high protein high animal diet group (HPA, 14.40$\pm$0.89mg/day). Apparent absorption and bioavailability of iron was also significantly higher(p<0.10) in HPA(40.7$\pm$5.3%, 6.46$\pm$1.61mg/day) than in HPP(14.4$\pm$5.3%, 2.39$\pm$0.89mg/day). But there was no significant difference between the high animal protein group and high plant protein group in moderate protein period. Serum iron concentration and transferrin saturation increased as animal protein intake increased, from 106.0$\pm$5.1ug/이 and 30.6$\pm$1.5% for MPA to 129.1$\pm$6.7ug/이 and 37.1$\pm$1.3% for HPA. Statistically positive correlations were shown not only between the level of dietary heme iron and apparent absorption(r=0.95, p<0.05), but also between serum iron concentration and apparent absorption(r=0.64, p<0.05). Negative iron balance was shown in two subjects fed the moderate protein meals. These results suggest that recommanded dietary allowances of iron may be under the need to maintain the positive balance, and iron bioavaliability increase by only high level of animal protein intake.

• Economic and Environmental Geology
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• v.33 no.4
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• pp.273-282
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• 2000

In order to investigate the extent and degree of arsenic and heavy metal contamination and the bioavailability of toxic elements around the abandoned mine in Korea, an environmental geochemical survey was undertaken in the Daduk mine. After appropriate preparation, tailings, soil, stream sediment, crop plant and fingernail samples were analysed for As, Cd, Cu, Pb and Zn by ICP-AES and ICP-MS. Elevated levels of 8,782 mg/kg As, 8.3 mg/kg Cd, 489 mg/kg Cu, 3,638 mg/kg Pb and 919 mg/kg Zn were found in tailings from the Daduk mine. These significant concentrations can impact on soils and sediments around the tailing ponds. Mean concentrations of As, Cd, Pb, Cu and Zn in soils are significantly higher than those in world average soil, especially for As and Pb. Element concentrations in sediments decrease with distance from the tailing ponds due to a dilution effect by the mixing of uncontaminated sediments. Arsenic and Cd are elevated in rice grains and stalks, and Cu and Zn concentrations in chinese cabbage, sesame and bean leaves are higher than the upper limit values for normal plant. Arsenic concentration in fingernails of farmers are higher than the normal level with a maximum value of 1.5 mg/kg. The post-ingestion bioavailability of toxic heavy metals in some paddy and farmland soils has been also investigated using the SBET (simple bioavailability extract test) method. The method utilises synthetic leaching fluids closelyanalogous to those of the human stomach. The quantities of As, Cd, Cu, Pb and Zn extracted from paddy soils after 1 hour indicated 15.9, 65.4, 46.2, 39.4 and 29.4% bioavailability, respectively and for farmland soils, 12.4, 26.0, 31.2, 29.3 and 19.4% bioavailability, respectively. The results of the SBET indicate that regular ingestion of soils by the local population could pose a potential health threat due to long-term toxic element exposure.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.551-566
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• 1997

Phosphorus has been known as an essential component of animal body. However, the requirement has not been determined precisely because of the variable bioavailabilities of feedstuffs from plant origin. The bioavailability of P in various feedstuffs of plant origin varies from 10 to 60%. Digestibility and availability of the P differed considerably depending on the feed. The lowest values were found for maize (under 20%), the highest for wheat and triticale (over 50%). This is due to the proportion of phytate and the presence of intrinsic phytase. And the digestive tract of monogastric animals does not contain sufficient amounts of phytase, an enzyme that hydrolyses the unavailable phytate complexes to available, inorganic orthophosphates. Microbial phytase supplementation improves the P availability, and both intrinsic plant and microbial phytase improves the availability of P in feedstuffs of plant origin. In a mixture of feeds with low and high activity of intrinsic phytase and/or supplemented by commercial phytase, the P availability is additive. However, in the light of current results it seems that exceeding the P availability equal to 60-70% is unrealizable even at large microbial phytase doses.

• Applied Microscopy
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• v.47 no.3
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• pp.148-156
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• 2017

The wide distribution of redox-sensitive elements (RSE) as arsenic (As), cadmium (Cd), selenium (Se), and strontium (Sr) in the top soil of Siwaqa area are related to the weathering action of alkaline surface and groundwater on the parent rocks. The bioavailability, distribution, sorption, and ecotoxicity of As, Cd, Se, and Sr, of the wild plants and top soils in the study area were investigated. A total number of 23 surface soil samples and 23 plant samples were collected and analyzed for the most toxic elements. The uptake of elements by plants was dependent on the plant species and the concentration of elements in the soil. For example, Sr was the highest concentration in soil samples and plants, while Se was the lowest concentration in soil samples and pants. For the plants, the results showed that Bellevalia sp. had the highest elements uptake, while Allium rothii had the lowest elements uptake. The results of this work provide a valuable knowledge for understanding the bioavailability of some toxic elements in the soil and plants of Central Jordan. The results are expected to be of great help for the Jordanian Uranium Mining Company during their environmental risk assessments.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.70-70
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• 2001

• Journal of Environmental Science International
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• v.26 no.7
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• pp.847-858
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• 2017

For 26 soil series distributed more than 1% among 63 soil series in Jeju Island, natural uncultivated soil samples were collected. For these soils, the chemical speciation of eight heavy metals (Cd, Cr, Cu, Mn, Ni, Pb, V, and Zn) was examined. Further, the Plant Bioavailability (PB) and Mobility Factor (MF) of these heavy metals were evaluated using Tessier's 5-step sequential extraction method (exchangeable, carbonate, reducible (bound to Fe/Mn oxides), oxidizable (bound to organic matter), and residual fraction). The main form present was residual fraction for Cd and Zn; residual and oxidizable fractions for Cr, Cu, Ni, and Pb; reducible fraction for Mn; and carbonate fraction for V. The average plant availability and average mobility factor were found to be V (57.37%) > Zn (12.49%) > Cd (11.76%) > Cu (11.19%) > Pb (9.37%) > Cr (9.09%) > Mn (3.13%) > Ni (2.63%), and Mn (61.04%) > V (59.94%) > Zn (31.54%) > Cd (17.65%) > Cr (15.66%) > Ni (13.89%) > Pb (13.80%) > Cu (13.53%), respectively.

• Journal of Soil and Groundwater Environment
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• v.24 no.6
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• pp.16-25
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• 2019

Anthropogenic polycyclic aromatic hydrocarbons (PAHs) are formed by the incomplete combustion of fuels and industrial waste. PAHs can be widely exposed to the environment (water, soil and groundwater). PAHs are potentially toxic, mutagenic and/or carcinogenic. Fundamental studies such as biota uptake (e.g., earthworm and plant) of PAHs are highly needed. It is necessary to develop alternative ways to evaluate bioavailability of PAHs instead of using living organisms because it is time-consuming, difficult to apply in the field, and also exaction method is tedious and time-consuming. In this study, sorption behaviors of phenanthrene were evaluated to predict the fate of PAHs in soils. Moreover, bioaccumulation of PAHs in an artificially contaminated soil was evaluated using pea plant (Pisum sativum) as a bioindicator. A novel passive sampler, organic-diffusive gradient in thin-film (o-DGT) for PAHs was newly synthesized, tested as a biomimic surrogate and compared with plant accumulation. Sorption partitioning coefficient (K_P) and sorption capacity (K_F) were in the order of natural soil > loess corresponding to the increase in organic carbon content (f_oc). Biota-to-soil accumulation factor (BSAF) and DGT-to-soil accumulation factor (DSAF) were evaluated. o-DGT uptake was linearly correlated with pea plant uptake of phenanthrene in contaminated soil (R²=0.863). The Tenax TA based o-DGT as a biomimic surrogate can be used for the prediction of pea plant uptake of phenanthrene in contaminated soil.

• Nutrition Research and Practice
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• v.8 no.5
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• pp.550-557
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• 2014

BACKGROUND/OBJECTIVES: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids. This study examined bioavailability of flavonoids and carotenoids in Angelica keiskei and the alteration of the antioxidant performance in vivo. SUBJECTS AND MATERIALS: Absorption kinetics of phytochemicals in Angelica keiskei were determined in healthy older adults (> 60 y, n = 5) and subjects with metabolic syndrome (n = 5). Subjects consumed 5 g dry Angelica keiskei powder encapsulated in gelatin capsules with a low flavonoid and carotenoid liquid meal. Plasma samples were collected at baseline, 0.5, 1, 2, 3, 4, 5, 6, 7, and 8 h. Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry. RESULTS: After ingestion of Angelica keiskei increases in plasma quercetin concentrations were observed at 1-3 and 6-8 hr in the healthy group and at all time points in the metabolic syndrome group compared to baseline (P < 0.05). Plasma lutein concentrations were significantly elevated in both the healthy and metabolic syndrome groups at 8 hr (P < 0.05). Significant increases in total antioxidant performance were also observed in both the healthy and the metabolic syndrome groups compared to baseline (P < 0.05). CONCLUSIONS: Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.04a
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• pp.37-37
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• 2002