• Title/Summary/Keyword: Plant Response

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Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

  • Kamran, Muhammad;Javed, Nazir;Ullah, Ihsan;Nazir, Shahid;Jamil, Shakra;Iqbal, Muhammad Zafar;Abbas, Huma;Khan, Sajid Aleem;Haq, Muhammad Ehetisham ul
    • The Plant Pathology Journal
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    • v.35 no.1
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    • pp.51-62
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    • 2019
  • A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.

Cytological Modification of Sorghum Leaf Tissues Showing the Early Acute Response to Maize Dwrf Mosaic Virus

  • Choi, Chang-Won
    • Journal of Plant Biology
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    • v.39 no.3
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    • pp.215-221
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    • 1996
  • Sorghum leaf tissues showing the early acute response of systemic infection with maize dwarf mosaic virus (MDMV) strain A, contained unusual virus-induced cytological modifications including cell wall thickenings and protrusions, intercellular vesicles termed as "paramural bodies", modified plasmodesmata, abnormal plastids, and cylindrical inclusion bodies. Abnormal cell wall, some of which associated with paramural bodies, was frequently contained modified plasmodesmata. Various abnormal plastids were located within infected cells of leaf tissues showing the early acute response. The most important changes in chloroplast seen in the tissues are the presence of small vesicles, deformation of membranes, reduction in granal stack height, disappearance of osmiophilic globules and degeneration of stuctures. The cytological modification was not occurred in nucleus but a group of degenerated mitochondria with abnormal membranes attached to cylindrical inclusion bodies were observed. It was hard more or less to prove the relationship clearly between virus and cellular organelles in virus replication.plication.

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Development of Herbicide Resistant Plant Through Plant Tissue Culture (제초제(除草劑) Butachlor 및 Simetryne에 저항성(抵抗性)인 식물체(植物體) 선발육성(選拔育成))

  • Kim, K.U.;Kim, S.H.
    • Korean Journal of Weed Science
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    • v.7 no.2
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    • pp.200-207
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    • 1987
  • This study was conducted to select and develop herbicide resistant plant through tissue culture. Growth response of seedlings and callis of various rice varieties with Echinochloa species was assessed under the treatment of various rates of butachlor [N-(butoxy methyl)-2-chloro-2', 6'-diethyl acetamide] and simetryne [2,4-bis(ethyl amino)-6-methyl thio-1,3,5-triazine]. Further, succinate dehydrogenase activity was determined in herbicide treated callus to characterize different response of plants to herbicide. Rice variety like Sangpung showed relative resistance in both callus and seedling states against butachlor, indicating maintenance of resistance. However, in the simetryne treatment, the similar response was not observed in callus and seedling state, although there was a great different response among plant materials against simetryne. Rice variety which exhibited resistance in callus and seedling states showed low succinate dehydrogenase inhibition index. Succinate dehydrogenase inhibition index can be used as an important marker characters to differentiate varietal response of plant to herbicide. Rice plant was differentiated from butachlor and simetryne tolerant callus treated at $2.5{\times}10^{-5}$ M is growing under the growth chamber and can be used for resistant source.

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Use of Plant Leaf in Biosensing for Some Odour Compounds

  • Matsuoka, Hideaki
    • Korean Journal of Pharmacognosy
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    • v.20 no.4
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    • pp.205-214
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    • 1989
  • The sensing of odour compounds in gas phase is an attractive target in recent sensor technology. Based on the finding that a plant leaf can respond to various gas molecules by changing its potential, biosensing system using a plant leaf has been investigated for the detection of odour compounds. A leaf of some plant species responded to odour compounds directly by changing its potential $5{\sim}10\;mV$. That the leaf was actually sensing an odour was much more remarkably detected from the difference between the response profile to pure $CO_2$ gas and that to $CO_2$ gas containing odour compounds. Then the quantitative study (ppb level) is now being performed on the response of a tobacco leaf to benzyl acetate; a component of jasminelike odours. The concept of biosensing and its significance are also described from the viewpoint of sensor technology.

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Seismic Analysis on a Control Panel of (Nuclear) Power Plant (발전소 주 제어실 제어패널의 내진해석)

  • Lee, Heung-Shik;Kim, Myung-Gu;Cho, Chongdu
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.15 no.6 s.99
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    • pp.652-659
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    • 2005
  • In this paper, seismic qualification analysis for the Plant control Panel is carried out to confirm the structural integrity under the seismic conditions represented by required response spectra(RRS). The finite element method(FEM) is used for the analysis and a mode combination method is adopted to obtain a more reliable spectrum analysis results. In addition, the experimental analysis is performed to compare the reliability of the analytical results. The analysis results shows that the plant control panel system is designed to have the dynamic rigidity with no resonance frequency below 33 Hz. The analytically calculated maximum stress of the plant control panel system is $36\%$ of the field strength of material, thus it can be shown that the system has a stable structure for the seismic load.

Test of Intermediate Disturbance Hypothesis by Experimental disturbance Gradient in Old-Field Plant Community (묵밭에서 교란처리구배에 따른 중간교란가설의 검증)

  • 이규송;김준호
    • The Korean Journal of Ecology
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    • v.21 no.3
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    • pp.233-241
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    • 1998
  • In order to test of intermediate disturbance hypothesis that an intermediate level of disturbance maximize biodiversity and to elucidate the effect of disturbance during the early old-field succession, the response of plant community along an experimental disturbance gradient was investigated in a five-year old-field. Response of plant community along an experimental disturbance gradient was connected with light condition because artificial disturbance gradient had been treat by clipping of plants and removing of litter. Artificial disturbance in five-year old-field plant community retarded rate of succession by increasing invasion and performance of the earlier successional species in the initial and middle stage of disturbance treatment. The species richness in the blocks of intermediate disturbance level showed the peak in early and mid-summer. This result did correspond with the prediction of intermediate disturbance hypothesis.

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Expression of $HpaG_{Xooc}$ Protein in Bacillus subtilis and its Biological Functions

  • Wu, Huijun;Wang, Shuai;Qiao, Junqing;Liu, Jun;Zhan, Jiang;Gao, Xuewen
    • Journal of Microbiology and Biotechnology
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    • v.19 no.2
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    • pp.194-203
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    • 2009
  • $HpaG_{Xooc}$, from rice pathogenic bacterium Xanthomonas oryzae pv. oryzicola, is a member of the harpin group of proteins, eliciting hypersensitive cell death in non-host plants, inducing disease and insect resistance in plants, and enhancing plant growth. To express and secret the $HpaG_{Xooc}$ protein in Bacillus subtilis, we constructed a recombinant expression vector pM43HF with stronger promoter P43 and signal peptide element nprB. The SDS-PAGE and Western blot analysis demonstrated the expression of the protein $HpaG_{Xooc}$ in B. subtilis. The ELISA analysis determined the optimum condition for $HpaG_{Xooc}$ expression in B. subtilis WBHF. The biological function analysis indicated that the protein $HpaG_{Xooc}$ from B. subtilis WBHF elicits hypersensitive response(HR) and enhances the growth of tobacco. The results of RT-PCR analysis revealed that $HpaG_{Xooc}$ induces expression of the pathogenesis-related genes PR-1a and PR-1b in plant defense response.