• 상하수도학회지
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• 제24권4호
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• pp.463-474
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• 2010

In this study, chemical coagulation conditions for treating combined sewer overflow(CSO) occurred during rainy season were evaluated by jar tests with aluminum sulfate[$Al_2(SO_4)_3{\cdot}17H_2O$] and ferric chloride[$FeCl_3{\cdot}6H_2O$]. The raw domestic sewage sampled from the primary sedimentation tank at a local sewage treatment plant was filtered through $150{\mu}m$ sieve before using. Point of zero charge(PZC) for various dose of aluminum sulfate occurred at pH 5.8-6.5, while for ferric chloride occurred at pH 5.3-6.0 in term of streaming current(SC) values. Charge neutralization ability of aluminum sulfate was bigger than that of ferric chloride. Optimum pH and dose of aluminum sulfate and ferric chloride were 6.2, 0.438mM and 5.8, 0.925mM, respectively. Removal efficiencies of TCOD, turbidity, SS and TP were 75, 97, 95, 96% with aluminum sulfate and 74, 96, 98, 99% with ferric chloride at their optimum coagulation conditions. More efficient removal of SS, TP and small particles was possible with ferric chloride at optimum coagulation conditions. Both SC values and COD removal started to increase where soluble phosphorus was completely removed.

• 한국습지학회지
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• 제16권4호
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• pp.423-431
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• 2014

상수원과 같은 수계 수질 보호 및 농촌 지역 개발을 위해 마을하수도 보급이 필요하다. 본 연구에서는 안동 지역 마을하수도(11개소) 유입하수 특성 및 마을하수도에 도입된 처리 공법의 결과를 분석하였다. 안동 지역 마을하수도 유입하수는 도시지역에 비해 고농도의 유기물과 영양염류를 함유하고 있는 것으로 분석되었다. 안동시 마을하수도 공법은 안정적인 처리효율을 보였지만, 영양염류의 경우 유기물에 비해 solid retention time(SRT)와 food to microorganisms (F/M) ratio에 민감한 것으로 나타났다. 또한 안동 지역 마을하수도 공법은 타 공법($A^2/O$, SBR, media)들과 비교하여 전반적으로 높은 처리 효율을 보였으며, 본 공법은 마을하수도에 효과적으로 적용 가능한 공법이라 판단된다.

• Genomics & Informatics
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• 제19권4호
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• pp.46.1-46.11
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• 2021

Moringa oleifera is nowadays raising as the most preferred medicinal plant, as every part of the moringa plant has potential bioactive compounds which can be used as herbal medicines. Some bioactive compounds of M. oleifera possess potential anti-cancer properties which interact with the apoptosis protein p53 in cancer cell lines of oral squamous cell carcinoma. This research work focuses on the interaction among the selected bioactive compounds derived from M. oleifera with targeted apoptosis protein p53 from the apoptosis pathway to check whether the bioactive compound will induce apoptosis after the mutation in p53. To check the toxicity and drug-likeness of the selected bioactive compound derived from M. oleifera based on Lipinski's Rule of Five. Detailed analysis of the 3D structure of apoptosis protein p53. To analyze protein's active site by CASTp 3.0 server. Molecular docking and binding affinity were analyzed between protein p53 with selected bioactive compounds in order to find the most potential inhibitor against the target. This study shows the docking between the potential bioactive compounds with targeted apoptosis protein p53. Quercetin was the most potential bioactive compound whereas kaempferol shows poor affinity towards the targeted p53 protein in the apoptosis pathway. Thus, the objective of this research can provide an insight prediction towards M. oleifera derived bioactive compounds and target apoptosis protein p53 in the structural analysis for compound isolation and in-vivo experiments on the cancer cell line.

• 한국토양비료학회지
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• 제47권6호
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• pp.464-472
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• 2014

The Codex Committee of Contaminants in Food (CCCF) has been discussing a new standard for arsenic (As) in rice since 2010 and a code of practice for the prevention and reduction of As contamination in rice since 2013. Therefore, our current studies focus on setting a maximum level of As in rice and paddy soil by considering bioavailability in the remediation of As contaminated soils. This study aimed to select an appropriate single chemical extractant for evaluating the mobility of As in paddy soil and the bioavailability of As to rice. Nine different extractants, such as deionized water, 0.01 M $Ca(NO_3)_2$, 0.1 M HCl, 0.2 M $C_6H_8O_7$, 0.43 M $HNO_3$, 0.43 M $CH_3COOH$, 0.5 M $KH_2PO_4$, 1 M HCl, and 1 M $NH_4NO_3$ were used in this study. Total As content in soil was also determined after aqua regia digestion. The As extractability of the was in the order of: Aqua regia > 1 M HCl > 0.5 M $KH_2PO_4$ > 0.43 M $HNO_3$ > 0.2 M $C_6H_8O_7$ > 0.1 M HCl > 0.43 M $CH_3COOH$ > deionized water > 1 M $NH_4NO_3$ > 0.01 M $Ca(NO_3)_2$. Correlation between soil extractants and As content in rice was in the order of : deionized water > 0.01 M $Ca(NO_3)_2$ > 0.43 M $CH_3COOH$ > 0.1 M HCl > 0.5 M $KH_2PO_4$ > 1 M $NH_4NO_3$ > 0.2 M $C_6H_8O_7$ > 0.43 M $HNO_3$ > 1M HCl > Aqua regia. BCF (bioconcentration factor) according to extractants was in the order of : 0.01M $Ca(NO_3)_2$ > 1 M $NH_4NO_3$ > deionized water > 0.43 M $CH_3COOH$ > 0.1 M HCl > 0.43 M $HNO_3$ > 0.2 M $C_6H_8O_7$ > 0.5 M $KH_2PO_4$ > 1 M HCl > Aqua regia. Therefore, 0.01 M $Ca(NO_3)_2$ ($r=0.78^{**}$) was proven to have the greatest potential for predicting As bioavailability in soil with higher correlation between As in rice and the extractant.

• Applied Biological Chemistry
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• 제21권1호
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• pp.58-62
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• 1978

인삼(人蔘)의 연근별(年根別) 건물중(乾物重)과 삼요소(三要素) 흡수량(吸收量)및 기타(其他)미량원소등(微量元素等)을 분석(分析)하여 인삼식물(人蓼植物)의 양분(養分) 요구상(要求相)과 영양생리(營養生理) 연구(硏究)의 기초자료(基礎資料)를 얻고자 과천인삼시험장(果川人蔘試驗場) 산(産) $1{\sim}6$년근(年根) 인삼(人蔘)을 대상(對象)으로 조사(調査)한 결과(結果)는 다음과 같다. 1. 인삼(人蔘)의 건물중(乾物重) 및 T/R율 T/R율은 저년근(低年根)이 적었고 4년근(四年根)이 0.52로 가장컸다. 이는 4년근(四年根)에서 지상부(地上部) 생육신장(生育伸長)이 왕성(旺盛)하기 때문인 것으로 사료(思料)되며 지상부(地上部) 및 근(根)의 건물중(乾物重)도 4년근(年根)에서 현저하게 증가(增加)하였다. 2. 인삼(人蔘)의 무기성분(無機成分)의 함량(含量) 가. 질소(窒素)는 엽근(葉根)에 비(比)해 경(莖)이 적었고 인산(燐酸)은 근(根), 경(莖), 엽순(葉順)으로 많으며 가리(加里)는 각년근(各年根) 공히 경(莖)에 많았고 특(特)히 N.P에 비(比)해 가리(加里)의 함량(含量)이 많았다. 나. 석회(石灰), 고토(苦土)는 엽(葉)과 경(莖)에 많았고 Fe, Mn은 각년근(各年根) 공히 엽(葉)에 많은 경향이며 Fe는 엽(葉)에 $1000{\sim}2000ppm$, 근(根) $350{\sim}750ppm$ Mn은 근(根)에 $40{\sim}80ppm$정도 함유(含有)되어 있었다. 다. Zn, Mn의 함량(含量)이 높은것은 Zn Mn 성분(成分)을 함유(含有)한 다이젠 약제살포(藥劑撤布)에 기인(基因)된 것으로 추측된다. 3. 6년근(年根)까지의 삼요소(三要素) 총흡수량(總吸收量)은 N;10.24kg, $P_2O_5;2.31kg$, $K_2O;14.86kg$으로서 가리(加里)의 흡수량(吸收量)이 대단히 많았다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 제9차 국제심포지움 및 추계정기학술발표회
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• pp.53-53
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• 2002

Shoot tips of in vitro propagated plantlets were cryopreserved using encapsulation/dehydration procedures. Shoot tips were excised under filter sterilized antioxidants solution (0.2M phosphate buffer, pH 5.7 supplemented with 5g/1 ascorbic acid and 15g/1 sodium borate). They were drawn up into a sterile 10 $\textrm{cm}^3$disposable pipette and were dropped into the culture medium with 2.5w/v Na-alginate, then into 100mM CaCl$_2$.2$H_2O$. Encapsulated shoot tips were transferred into 10㎤ of liquid culture medium with a range of sucrose concentrations (0.25-1.0M) and were incubated in dark for 24 hours in 18C at 40rpm. Beads were then dehydrated in silica gel for different time intervals (1-24 hours). Then they were freeze dried either rapidly (plunge directly into liquid N2 or in two stages (samples were kept at 20C for 10 minutes, then reduced to 35C at 1C per minute. Then, plunge into liquid $N_2$). The influence of sucrose and silica gel pre-treatment on pre- and post-freeze shoot growth was examined.(중략)

• 식물분류학회지
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• 제46권3호
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• pp.306-313
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• 2016

전북 진안군 금강변에서 비비추속의 신변종인 금강비비추(Hosta clausa Nakai var. geumgangensis M. Kim & H. Jo)를 새로이 발견하여 기재하였다. 금강비비추는 근경에 기는 줄기가 있고, 종주선이 화경에 없으며, 약은 자색이라는 점에서 비비추 H. clausa var. normalis나 참비비추 H. clausa var. clausa와 유사하다. 그러나 금강비비추는 개방화이고, 종자 결실을 하지 않으며, 엽병이 녹색이며, 암술과 수술의 길이가 비슷하고, 화피 협통부는 길이가 짧다. 반면에, 비비추는 개방화이고, 종자 결실을 하며, 엽병은 자색 반점이 있으며, 암술은 수술보다 길고, 화피 협통부는 길이가 길다. 또한 참비비추는 꽃의 화피가 전혀 열리지 않는 폐쇄화이고 종자를 맺지 못한다.

• BMB Reports
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• 제41권11호
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• pp.771-777
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• 2008

Calmodulin (CaM) proteins, members of the EF-hand family of $Ca^{2+}$-binding proteins, represent important relays in plant calcium signals. Here, OsCam1-1 was isolated by PCR amplification from the rice genome. The gene contains an ORF of 450 base pairs with a single intron at the same position found in other plant Cam genes. A promoter region with a TATA box at position-26 was predicted and fused to a gus reporter gene, and this construct was used to produce transgenic rice by Agrobacterium-mediated transformation. GUS activity was observed in all organs examined and throughout tissues in cross-sections, but activity was strongest in the vascular bundles of leaves and the vascular cylinders of roots. To examine the properties of OsCaM1-1, the encoding cDNA was expressed in Escherichia coli. The electrophoretic mobility shift when incubated with $Ca^{2+}$ indicates that recombinant OsCaM1-1 is a functional $Ca^{2+}$-binding protein. In addition, OsCaM1-1 bound the CaMKII target peptide confirming its likely functionality as a calmodulin.

• Journal of Plant Biotechnology
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• 제45권4호
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• pp.340-346
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• 2018

In this study, the effect of elicitors such as yeast extract (YE), methyl jasmonate (MeJA) and salicylic acid (SA) on the accumulation of eurycomanone in Eurycoma longifolia cell cultures were investigated. Suspension cells of E. longifolia was cultured in Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose, 1.25 mg/L naphthaleneacetic acid (NAA) and 1 mg/L kinetin at a shaking speed of 120 rpm. Elicitors were added in the culture at different concentrations and times to stimulate eurycomanone accumulation in the Eurycoma longifolia cells. Eurycomanone content was determined by HPLC with a C18 column, flow rate of 0.8 mL/min, run time of 17.5 min, and a detector wavelength of 254 nm. The stationary phase was silica gel and the mobile phase was acetonitrile: $H_2O$. Non-elicited cells were used as the control. The study showed the effect of different elicitor concentrations, YE at 200 mg/L, MeJA at $20{\mu}M$ and SA at $20{\mu}M$ stimulated high production of eurycomanone. In which, treatment of $20{\mu}M$ MeJA after 4 days of culture resulted in the highest accumulation of this compound (17.36 mg/g dry weight), approximately 10-fold higher than that of untreated cells (1.70 mg/g dry weight).

• Journal of Ginseng Research
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• 제13권1호
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• pp.84-91
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• 1989

인삼재배산지에서 연근별 토양이화학성과 이삼생육변화를 조사하여 토성별 생육특성을 파악하고 6년근 수량과 결주율에 미치는 토양이화학성의 영향을 구명코저하였다. 1. 산지 6년근 수량은 토성별고 사양토 1.44, 양토 2.13, 식양토 2.46kg/3.3$m^2$이었다. 2. 6년근 결주율은 사양토 51.6%, 식양토 33.6%이었고 묘직경은 3년근부터 사양토가 식양토보다 적었다. 3. 토양입단율과 공극율은 2년근 때보다 6년근에서 증가하였다. 4. 토양무기태질소는 2, 3년근에서 100-120ppm으로 현저히 증가하였다가 4년근 75ppm, 5년근 34ppm, 6년근 25ppm으로 감소하였으며 사양토에서 감소폭이 컸다. 5. 본 포에서 $P_2O_5$, K, Ca, Mg는 연차간변화가 적었고 토성별로는 사양토가 N, P 함량은 높았고 양이온 함량은 낮았다. 6. 6년근 수량은 점토, 공극율, 수분과 유의성있는 정상관이었고 결주율은 모래, N 함량과 정상관이었다.