• Journal of Korean Society of Water and Wastewater
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• v.24 no.4
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• pp.463-474
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• 2010

In this study, chemical coagulation conditions for treating combined sewer overflow(CSO) occurred during rainy season were evaluated by jar tests with aluminum sulfate[$Al_2(SO_4)_3{\cdot}17H_2O$] and ferric chloride[$FeCl_3{\cdot}6H_2O$]. The raw domestic sewage sampled from the primary sedimentation tank at a local sewage treatment plant was filtered through $150{\mu}m$ sieve before using. Point of zero charge(PZC) for various dose of aluminum sulfate occurred at pH 5.8-6.5, while for ferric chloride occurred at pH 5.3-6.0 in term of streaming current(SC) values. Charge neutralization ability of aluminum sulfate was bigger than that of ferric chloride. Optimum pH and dose of aluminum sulfate and ferric chloride were 6.2, 0.438mM and 5.8, 0.925mM, respectively. Removal efficiencies of TCOD, turbidity, SS and TP were 75, 97, 95, 96% with aluminum sulfate and 74, 96, 98, 99% with ferric chloride at their optimum coagulation conditions. More efficient removal of SS, TP and small particles was possible with ferric chloride at optimum coagulation conditions. Both SC values and COD removal started to increase where soluble phosphorus was completely removed.

• Journal of Wetlands Research
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• v.16 no.4
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• pp.423-431
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• 2014

Supply of rural community sewage treatment plant (RCSTP) is need for protect of water quality like water source and development of agriculture areas. In this study conducted analysis on characteristics of RCSTP sewage and treatment efficiency of installed treatment process using operation results of RCSTP in an-dong area. high organic matter and nutrient were contained in RCSTP sewage of an-dong than urban area. RCSTP treatment process in an-dong was shown stable treatment efficiency. But nutrient treatment efficiency was shown more sensitive result about SRT and F/M ratio than organic matter treatment efficiency. And RCSTP treatment process in an-dong was shown higher treatment efficiency than other treatment proces like $A^2/O$, SBR and media. Thus, this process is effective treatment process for application of RCSTP.

• Genomics & Informatics
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• v.19 no.4
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• pp.46.1-46.11
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• 2021

Moringa oleifera is nowadays raising as the most preferred medicinal plant, as every part of the moringa plant has potential bioactive compounds which can be used as herbal medicines. Some bioactive compounds of M. oleifera possess potential anti-cancer properties which interact with the apoptosis protein p53 in cancer cell lines of oral squamous cell carcinoma. This research work focuses on the interaction among the selected bioactive compounds derived from M. oleifera with targeted apoptosis protein p53 from the apoptosis pathway to check whether the bioactive compound will induce apoptosis after the mutation in p53. To check the toxicity and drug-likeness of the selected bioactive compound derived from M. oleifera based on Lipinski's Rule of Five. Detailed analysis of the 3D structure of apoptosis protein p53. To analyze protein's active site by CASTp 3.0 server. Molecular docking and binding affinity were analyzed between protein p53 with selected bioactive compounds in order to find the most potential inhibitor against the target. This study shows the docking between the potential bioactive compounds with targeted apoptosis protein p53. Quercetin was the most potential bioactive compound whereas kaempferol shows poor affinity towards the targeted p53 protein in the apoptosis pathway. Thus, the objective of this research can provide an insight prediction towards M. oleifera derived bioactive compounds and target apoptosis protein p53 in the structural analysis for compound isolation and in-vivo experiments on the cancer cell line.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.464-472
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• 2014

The Codex Committee of Contaminants in Food (CCCF) has been discussing a new standard for arsenic (As) in rice since 2010 and a code of practice for the prevention and reduction of As contamination in rice since 2013. Therefore, our current studies focus on setting a maximum level of As in rice and paddy soil by considering bioavailability in the remediation of As contaminated soils. This study aimed to select an appropriate single chemical extractant for evaluating the mobility of As in paddy soil and the bioavailability of As to rice. Nine different extractants, such as deionized water, 0.01 M $Ca(NO_3)_2$, 0.1 M HCl, 0.2 M $C_6H_8O_7$, 0.43 M $HNO_3$, 0.43 M $CH_3COOH$, 0.5 M $KH_2PO_4$, 1 M HCl, and 1 M $NH_4NO_3$ were used in this study. Total As content in soil was also determined after aqua regia digestion. The As extractability of the was in the order of: Aqua regia > 1 M HCl > 0.5 M $KH_2PO_4$ > 0.43 M $HNO_3$ > 0.2 M $C_6H_8O_7$ > 0.1 M HCl > 0.43 M $CH_3COOH$ > deionized water > 1 M $NH_4NO_3$ > 0.01 M $Ca(NO_3)_2$. Correlation between soil extractants and As content in rice was in the order of : deionized water > 0.01 M $Ca(NO_3)_2$ > 0.43 M $CH_3COOH$ > 0.1 M HCl > 0.5 M $KH_2PO_4$ > 1 M $NH_4NO_3$ > 0.2 M $C_6H_8O_7$ > 0.43 M $HNO_3$ > 1M HCl > Aqua regia. BCF (bioconcentration factor) according to extractants was in the order of : 0.01M $Ca(NO_3)_2$ > 1 M $NH_4NO_3$ > deionized water > 0.43 M $CH_3COOH$ > 0.1 M HCl > 0.43 M $HNO_3$ > 0.2 M $C_6H_8O_7$ > 0.5 M $KH_2PO_4$ > 1 M HCl > Aqua regia. Therefore, 0.01 M $Ca(NO_3)_2$ ($r=0.78^{**}$) was proven to have the greatest potential for predicting As bioavailability in soil with higher correlation between As in rice and the extractant.

• Applied Biological Chemistry
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• v.21 no.1
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• pp.58-62
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• 1978

Dry weight, and mineral content in leaves, stems, and roots of the ginseng plant (Panax ginseng C.A. Meyer) were investigated. The highest T/R ratio, 0.52, was observed in a four year old plant due to a vigorous growth of the plant at this age. Also the dry weight increase was rather higher in the younger root than the older one. Mineral content of the plant was rather higher in the younger root than the older one. Potassium content in leaves and stems was higher than those of nitrogen and phosphorus. Generally, the leaves contained more iron and manganese than any other micronutrients. The iron content amounted to $1,000{\sim}2,000ppm$ in leaves. Zinc content was higher in stems and leaves than roots especially in stems of the younger roots. Total amount of the up-taken N, $P_2O_5$, and $K_2O$ per 10a in six years was 10.2kg, 2.3kg and 14.9kg respectively, indicating the considerable abounts of $K_2O$ was absorbed.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.53-53
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• 2002

Shoot tips of in vitro propagated plantlets were cryopreserved using encapsulation/dehydration procedures. Shoot tips were excised under filter sterilized antioxidants solution (0.2M phosphate buffer, pH 5.7 supplemented with 5g/1 ascorbic acid and 15g/1 sodium borate). They were drawn up into a sterile 10 $\textrm{cm}^3$disposable pipette and were dropped into the culture medium with 2.5w/v Na-alginate, then into 100mM CaCl$_2$.2$H_2O$. Encapsulated shoot tips were transferred into 10㎤ of liquid culture medium with a range of sucrose concentrations (0.25-1.0M) and were incubated in dark for 24 hours in 18C at 40rpm. Beads were then dehydrated in silica gel for different time intervals (1-24 hours). Then they were freeze dried either rapidly (plunge directly into liquid N2 or in two stages (samples were kept at 20C for 10 minutes, then reduced to 35C at 1C per minute. Then, plunge into liquid $N_2$). The influence of sucrose and silica gel pre-treatment on pre- and post-freeze shoot growth was examined.(중략)

• Korean Journal of Plant Taxonomy
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• v.46 no.3
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• pp.306-313
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• 2016

A new variety, Hosta clausa Nakai var. geumgangensis M. Kim & H. Jo, was found in Geumgang River in Korea. This variety shares several characteristics (rhizomes with underground stolon, smooth scape and purple-colored anthers) with the $re{\acute{o}}lated$ varieties H. clausa Nakai var. clausa and H. clausa var. normalis F. Maekawa. However, H. clausa var. normalis has open flowers, capsules with seeds, purple-spotted petioles, long pistils, and long narrow perianth tubes, whereas this new variety has open flowers, seedless capsules, green-colored petioles, short pistils, and short narrow perianth tubes. Hosta clausa Nakai var. clausa has closed flowers and seedless capsules.

• BMB Reports
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• v.41 no.11
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• pp.771-777
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• 2008

Calmodulin (CaM) proteins, members of the EF-hand family of $Ca^{2+}$-binding proteins, represent important relays in plant calcium signals. Here, OsCam1-1 was isolated by PCR amplification from the rice genome. The gene contains an ORF of 450 base pairs with a single intron at the same position found in other plant Cam genes. A promoter region with a TATA box at position-26 was predicted and fused to a gus reporter gene, and this construct was used to produce transgenic rice by Agrobacterium-mediated transformation. GUS activity was observed in all organs examined and throughout tissues in cross-sections, but activity was strongest in the vascular bundles of leaves and the vascular cylinders of roots. To examine the properties of OsCaM1-1, the encoding cDNA was expressed in Escherichia coli. The electrophoretic mobility shift when incubated with $Ca^{2+}$ indicates that recombinant OsCaM1-1 is a functional $Ca^{2+}$-binding protein. In addition, OsCaM1-1 bound the CaMKII target peptide confirming its likely functionality as a calmodulin.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.340-346
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• 2018

In this study, the effect of elicitors such as yeast extract (YE), methyl jasmonate (MeJA) and salicylic acid (SA) on the accumulation of eurycomanone in Eurycoma longifolia cell cultures were investigated. Suspension cells of E. longifolia was cultured in Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose, 1.25 mg/L naphthaleneacetic acid (NAA) and 1 mg/L kinetin at a shaking speed of 120 rpm. Elicitors were added in the culture at different concentrations and times to stimulate eurycomanone accumulation in the Eurycoma longifolia cells. Eurycomanone content was determined by HPLC with a C18 column, flow rate of 0.8 mL/min, run time of 17.5 min, and a detector wavelength of 254 nm. The stationary phase was silica gel and the mobile phase was acetonitrile: $H_2O$. Non-elicited cells were used as the control. The study showed the effect of different elicitor concentrations, YE at 200 mg/L, MeJA at $20{\mu}M$ and SA at $20{\mu}M$ stimulated high production of eurycomanone. In which, treatment of $20{\mu}M$ MeJA after 4 days of culture resulted in the highest accumulation of this compound (17.36 mg/g dry weight), approximately 10-fold higher than that of untreated cells (1.70 mg/g dry weight).

• Journal of Ginseng Research
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• v.13 no.1
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• pp.84-91
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• 1989

This study was conducted to Investigate the effects of physico-chemical soil properties on the growth and yield of ginseng. 1 In the field survey, the yields of 6 year old ginseng were 2.46 Kg/3.3$m^2$, 2.13 Kg/3.3$m^2$, 1.44 Kg/3.3 $m^2$ in clay loam, loam and sandy loam soils, respectively 2. The missing plant rate for il year old ginseng were 33.6% and 51.6% in clay loam and sandy loam soils, respectively : the stem length and stem diameter of ginseng plants in sandy loam soil were smaller than those in clay loam soil. 3. Soil aggregation and porosity we're slightly higher in 6 year old ginseng fields than in 2 year ones. 4. Inorganic-N increased in 2 year and 3 year old ginseng fields reaching up to 100-120ppm, however it 1 decreased to 75 ppm, 34 ppm and 25 ppm in 4, 5 and 6 year old ginseng fields, respectively, It varied 1 more greatly in sandy loam soil than in clay loam. 5. The $P_2O_5$, K, Ca, and Mg contents differed little with plant age. Sandy loam had high N and $P_2O_5$ contents but low cation contents. 6. The yield of 6 year old ginseng fields were significantly correlated with clay contents and porosity. The missing Plant rate of 6 year old ginseng had a positive correlations with sand. and N contents.