• Title/Summary/Keyword: Plant Leaf Disease

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Studies on the Pear Abnormal Leaf Spot Disease - 2. Identification of Causal Agent - (배나무잎 이상반점증상에 관한 연구 - 2. 원인구명 -)

  • 남기웅;김충회
    • Korean Journal Plant Pathology
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    • v.11 no.3
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    • pp.210-216
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    • 1995
  • Alternaria spp.were predominantly isolated from the abnormal leaf spot lesions of pear cultivars Niitaka and Nijiiseiki. Alternaria isolates from the cultivar Niitaka were not pathogenic to both cultivars, but the isolates from the cultivar Nijiiseiki developed typical lesions of black leaf spots and were identified as A. kikuchiana. However, no typical abnormal leaf spot lesions were produced by the Alternaria isolates. Foliar spray of twelve different agrochemicals including lime sulfur, either alone or in combinations, with 7 times applications from April to July failed to reduced the disease development. Application of 17 different pesticides including fungicides, insecticides and herbicides currently used in pear orchards did not cause leaf injury similar to the abnormal leaf spot. Simulated acid rain of as low as pH 3.0 did not incite any leaf lesions alike the abnormal spot lesions. Mineral contents in the leaves of both cultivars did not differ significantly between the healthy leaves and those with abnormal leaf spots. When cuttings of pear tree were obtained in February from newly emerged twigs of the healthy or the diseased trees of Niitaka and planted in sand in the greenhouse, only those from the diseased trees developed typical leaf lesions of the abnormal spot. These results indicate that abnormal leaf spots are caused by unknown systemic agents in pear trees, rather than by Alternaria spp., chemical injury or acid rain.

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Cultural Characteristics of Mcyosphaerella nawae causing Spotted Leaf Casting of Persimmon (감나무 둥근무늬낙엽병균(Mycosphaerella nawae)의 배양적 특성)

  • 권진혁;강수웅;정부근;박창석
    • Plant Disease and Agriculture
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    • v.1 no.2
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    • pp.18-21
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    • 1995
  • Mycosphaerella nawae, the causal organism, of spotted leaf casing disease of persimmon, was isolated from infected leaves showing typical symptom. The cultural characteristics of the fungus were compared on artificial media. Among 24 different combinations of culture media and supplements, oatmeal agar+persimmon leaf extract (PLE) and PAD+ PLE+streptomycin showed the highest rate of isolating as 57.1%. The best medium for mycelial growth of the fungus was PDA+persimmon leaf powder (PLP). The colony diameter was reached 47mm for 30 days at 2$0^{\circ}C$. PDA+PLE also supported good mcyelial growth showing 46mm of diameter in same condition. The optimum growth temperature of this fungus in PDA was recognized fairly low. The mycelial growth was higher at 2$0^{\circ}C$ than 15$^{\circ}C$. The variation of pH between pH 4 to pH 8 did not affect to the mycelial growth of the pathogen.

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Differential Responses of Soybean Cultivars to Cercospora sojina Isolates, the Causal Agent of Frogeye Leaf Spot in Korea

  • Kim, Ji-Seong;Lee, Young-Su;Kim, Sung-Kee;Kim, Ki-Deok;Kim, Jin-Won
    • The Plant Pathology Journal
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    • v.27 no.2
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    • pp.183-186
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    • 2011
  • During the summer of 2005, specimens of soybean cultivars (Daepung, Daewon, Hwanggeum and Taegwang) showing frogeye leaf spot (FLS) were obtained from various areas in Korea. Eight isolates identified as Cercospora sojina were inoculated on the adaxial leaf surfaces of 63 Korean soybean cultivars; the disease responses to each isolate were evaluated 14 days and 21 days after inoculation. Based on the disease responses (resistant or susceptible) of the cultivars by the isolates, a set of cultivars (Anpeong, Bogwang, Cheongdu No. 1, Cheongja No. 3, Dachae, Daemang, Jangwon, Namhae, Sowon, Taegwang) were selected and inoculated with seven isolates for further testing pathogenic variance. Interestingly, 6 out of 7 tested C. sojina isolates revealed differential ability in infecting different soybean cultivars. This result may indicate the possibility of new race occurrence or pathogenic variation; this also presents evidence for prevalent FLS occurrence during humid and hot weather in Korea.

Incidence of Diseases in Codonopsis lanceolata with Different Cultivation Method (재배양식에 따른 더덕 병해 발생양상)

  • 김주희;최정식
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.676-681
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    • 1998
  • Disease incidence of Codonopsis lanceolata was surveyed at the major cultivating fields in Chonbuk province in 1996 to 1997. The main diseases of Codonopsis lanceolata were ovserved as leaf spot caused by Septoria codonopsis, anthracnose by Glomerella cingulata, brown leaf spot by Cercospora sp., rust by Coleosporium koreanum, powdery mildew by Erysiphe sp., Fusarium wilt caused by Fusarium oxyporum, and white root rot by Sclerotium rolfsii. Anthracnose, leaf spot and brown leaf spot occurred severely on leaves from early July to late August. They were caused early fallen leaves. Fusarium wilt and white root rot occurred severely on stem and below the soil line in late August. They resulted in withering to death or chlorosis and fallen of leaves. Disease incidence of Codonopsis lanceolata was also substantially different in occurrence with a method of cultivation in late growth stage. Fusarium wilt and white root rot were more severe with a method of no support cultivation than those with a method of support cultivation with a stick. Fusarium wilt occurred 48.8% in a method of no support cultivation but 3.1% in a method of support cultivation with a stick. And white root rot occurred 18.9% in a method of no support cultivation but 0.3% in a method of no support cultivation with a stick. Thus, it proved that soil-borne diseases could be controlled support cultivation with a stick.

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Incidence of Pear Black Necrotic Leaf Spot and its Control by Top-Grafting (배나무 잎검은점병의 피해상황과 방제)

  • Nam, Ki-Woong;Myung, Inn-Shik
    • Research in Plant Disease
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    • v.8 no.4
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    • pp.254-257
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    • 2002
  • It was conducted to investigate an effective control measure of the black necrotic leaf spot disease on pear, The disease commonly occurred in major cultivation areas of Korea revealed 5.6 % infection rate on the widely growing cultivar Niikata. The disease substantially reduced yield and quality of pear fruits, however, it has never been controlled by chemicals. Renewal of the pear trees to resistant cultivar was found to be the most effective to control the disease. As a result, the disease did not developed for 2 years on the diseased Niikata that was top-grafted with resistant cultivars Soowhang and Whasan. lop-grafting with resistant cultivars was recommended far the pear trees showing over 80% infection rate and lower than 70% fruit yield compare to standard production.

Combining-Ability and Heterosis for Mutant Character of Quantitative Characters in Flue-Cured Tobacco Varieties(Nicotiana tabacum L) (황색종 연초에 있어서 변이체의 조합능력 및 Heterosis)

  • Jeong, Seok-Hun;Lee, Seung-Cheol;Kim, Heung-Bae
    • Journal of the Korean Society of Tobacco Science
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    • v.15 no.1
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    • pp.34-48
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    • 1993
  • This experiment were conducted to investigate heterosis and combining ability for several mutant characters by analyzing dialled crosses of flue-cured tobacco. In a dialled cross of 3 flue-cured varieties and the mutant line 83H -5, the heterosis was somewhat higher in Fl than in F2. For growth character, the heterosis was 0.28-6.03% in plant height, leaf number, leaf shape index and yield, and was 43.2% for bacterial wilt disease index. The mutant line 83H-5 showed significantly negative GCA effect for plant height, leaf width and bacterial wilt disease index in Fl and F2, leaf length in F2, and positive GCA effect for total alkaloids, total nitrogen in Fl and days to flower in F2, respectively. Specific combining ability(SCA) in 83H-5 x Hicks was significant in negative effect for leaf length(F2), number of leaves(F2), leaf shape(F1, F2), bacterial wilt(F2) and alkaloids(F1), and in 83H-5 x NC 2326 in positive effect for leaf length(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in positive effect for plant height(F1, F2) and leaf width(F2), and for 83H-5 x NC 82 in Positive effect for Plant height(F1, F2), leaf length(F2) and yield(F1, F2).

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Disease Resistance Test Method of Cucumber Powdery Mildew(Sphaerotheca fusca) Using A Leaf Disk Assay (잎절편 (Leaf disk)을 이용한 오이 횐가루병 (Sphaerotheca fusca)에 대한 내병성 검정법)

  • Lee, Yong-Hwan;Seo, Jong-Bun;Choi, Kyong-Ju;Park, In-Jin;Yang, Won-Mo
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.78-81
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    • 2004
  • The resistance of 10 varieties of cucumber (Cucumis sativus L.) to powdery mildew, caused by Sphaerotheca fusca, was evaluated by a leaf disk assay. Leaf disks (10 mm in diameter) were removed from fully expanded leaves and then placed in petri dishes containing 0.16% water agar amended with benzimidazole. Leaf disks were inoculated by dropping a 10 $\mu$l of conidia suspension. Conidiophore formation of powdery mildew was the greatest at $25^{\circ}C$. The response of the host to powdery mildew, based on the inoculation onto disks of the first leaf, highly correlated with results obtained from harvesting stage of cucumber plants in greenhouse test (r = 0.99$^{**}$). It is indicating that a leaf disk assay may precisely predict the response of cucumber plant to S. fusca.a.

Leaf Spot of Rye Caused by Bipolaris sorokiniana in Korea

  • Chang, Seog-Won;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • v.16 no.6
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    • pp.325-327
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    • 2000
  • A leaf spot of rye (Secale cereale L.) was observed during the summer 1999 in Korea. A fungus associated with the disease was identified as Bipolaris sorokiniana (Sacc.) Shoem., based on the morphological characteristics of conidia and conidiophores. Pathogenicity of the fungus was proven by artificial inoculation on rye plants. This is the first record of leaf spot on rye caused by B. sorokiniana in Korea.

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Xylella fastidiosa in Europe: From the Introduction to the Current Status

  • Vojislav, Trkulja;Andrija, Tomic;Renata, Ilicic;Milos, Nozinic;Tatjana Popovic, Milovanovic
    • The Plant Pathology Journal
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    • v.38 no.6
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    • pp.551-571
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    • 2022
  • Xylella fastidiosa is xylem-limited bacterium capable of infecting a wide range of host plants, resulting in Pierce's disease in grapevine, citrus variegated chlorosis, olive quick decline syndrome, peach phony disease, plum leaf scald, alfalfa dwarf, margin necrosis and leaf scorch affecting oleander, coffee, almond, pecan, mulberry, red maple, oak, and other types of cultivated and ornamental plants and forest trees. In the European Union, X. fastidiosa is listed as a quarantine organism. Since its first outbreak in the Apulia region of southern Italy in 2013 where it caused devastating disease on Olea europaea (called olive leaf scorch and quick decline), X. fastidiosa continued to spread and successfully established in some European countries (Corsica and PACA in France, Balearic Islands, Madrid and Comunitat Valenciana in Spain, and Porto in Portugal). The most recent data for Europe indicates that X. fastidiosa is present on 174 hosts, 25 of which were newly identified in 2021 (with further five hosts discovered in other parts of the world in the same year). From the six reported subspecies of X. fastidiosa worldwide, four have been recorded in European countries (fastidiosa, multiplex, pauca, and sandyi). Currently confirmed X. fastidiosa vector species are Philaenus spumarius, Neophilaenus campestris, and Philaenus italosignus, whereby only P. spumarius (which has been identified as the key vector in Apulia, Italy) is also present in Americas. X. fastidiosa control is currently based on pathogen-free propagation plant material, eradication, territory demarcation, and vector control, as well as use of resistant plant cultivars and bactericidal treatments.

Occurence of Powdery Mildew Caused by Podosphaera xanthii on Vigna nakashimae in Korea (Podosphaera xanthii에 의한 좀돌팥 흰가루병 발생)

  • Kyung-Gu, Min;Tae-Min, Park;Youn-Jin, Park;Myoung-Jun, Jang
    • Research in Plant Disease
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    • v.28 no.4
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    • pp.248-251
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    • 2022
  • In October 2022, common Asian cowpea (Vigna nakashimae) powdery mildew disease was investigated at Yesan-gun, Chungcheongnam-do, Korea (36°40'12.7"N,126°51'36.4"E). The disease mainly occurred on the front of the leaves, and weak powdery mildew disease occurred on the back side of leaf. The chasmothecia was identified on the back side of the leaf. Analysis of the internal transcribed spacer rDNA sequence and 28S large subunit sequence, along with the morphological characteristics of the anamorph and teleomorph of the causal fungus identified as Podosphaera xanthii. This is the first time in Korea to report common Asian cowpea (Vigna nakashimae) powdery mildew disease caused by Podospaera xanthii.