• Title/Summary/Keyword: Plant Growth Regulators

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Initial Divisions of Microspores in In Vitro Cultured Anthers of Cultivated Paeonia albiflora (균약 약배양에 있어서 소포자의 초기분열)

  • 한창열
    • Journal of Plant Biology
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    • v.19 no.2
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    • pp.45-48
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    • 1976
  • Anthers of cultivated Paeonia albiflora were cultured on media supplemented with various combinations of growth regulators. Although the number of anthers with emerged calluses were very few, in the sectioned anthers were found many multinucleate, 2-celled, or multicellular microspores, the one-celled multinucleate microspores being most abundant in number, and the multicellular ones the least. In 2-celled or 3-celled microspores two kinds were observed: one is ordinary one with single nucleus in each cell, and the other is multinucleate one. Majority of the 2-celled microspores was found to be of equational-division irrespective of whether they were multinucleate micropores or ordinary nonmultinucleate ones.

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In vitro bioassay for allelopathic substances of Sorghum ( Sorghumbicolor L.) (수수로부터 allelopathy성 물질의 기내선별)

  • 유창연
    • Korean Journal of Plant Resources
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    • v.7 no.2
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    • pp.115-119
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    • 1994
  • These experiments were conducted to determine the effects of Sorghum allelopathic substances on the callus growh of several weeds and crops. 1. When substances extracted from allelopathic Sorghum(Sorghum bicolor L.) were treated on medium, growth of callus of several weeds and crops were in-hibited. The degree of inhibition differed depending on the genotypes, ranging from 50 to 90% com-pared with that of control. 2. The extracts of above 5% Sorghum inhibited the callus growth of Che-nopodium albun L., Commelina communis L., and .Ammaranthus retroflexus L.and showed in-hibition rate of above 70% in callus growth. These results indicate that we could investigate theallelopaihy effect by using in vitro system. 3. The suitable explant for callus induction fromallelopathic plants was immature embryos, the callus induction rate differed depending on the geno-type, growth regulators and concentrations. In general, the addition of 2, 4-D and NAA onto medium increased the rate and amount of callus.

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Rooting Characteristics of Sageretia thea by Cutting Time, Rooting Medium and Plant Growth Regulators (삽목시기, 삽목용토, 생장조절제에 따른 상동나무 삽목발근 특성)

  • Son, Yonghwan;Park, Sunghyuk;Son, Hojun;Kim, Ji-Ah;Cho, Hyejung;Lee, Sun-Young;Kim, Hyun-Jun
    • Korean Journal of Plant Resources
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    • v.35 no.5
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    • pp.667-674
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    • 2022
  • This study was conducted to determine the three main factors [rooting time, plant growth regulators (PGRs), rooting medium] of cuttage for mass production of Sageretia thea. The experiment was observed on cuttage tray after 120 days and classified by rooting times, plant growth regulators, rooting medium. Materials were collected from natural habitats of S. thea from the Korean peninsula at March, July and September during 2019. As a result of the experiment, rooting times were showed statistically significant differences. March group's rooting rate and root length were 15 and 200% better than others. In PGRs experiment by concentration, There was no significant correlation between the control and experiment in terms of rooting rate. However, In the case of root length, IBA 500 mg/L, IBA 250 mg/L and IAA 1,000 mg/L groups were lengthened up to 170% compared to the control group, confirming statistically significant differences. As a result, we recommend treating cuttings with IBA 500 mg/L in March with Hardwood cutting.

Vertical Shoot Growth of Korean Lawngrass (Zoysia japonica Steud.) Influenced by Trinexapac-ethyl, Amidochlor, and Mefluidide (Trinexapac-ethyl, Amidochlor 및 Mefluidide가 들잔디 직립생장에 미치는 효과)

  • Kim, Kyoung-Nam;Kim, Yong-Seon
    • Horticultural Science & Technology
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    • v.17 no.5
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    • pp.572-577
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    • 1999
  • Research was initiated to evaluate plant growth regulator effects on the vertical shoot growth of Korean lawngrass and to determine desirable growth regulator and its rate. The experiments were conducted twice at different sites in 1995. All the tested growth regulators inhibited the growth, but the inhibition period was variable among the product in Experiments I and II. During the first week after treatment, there was approximately 10 to 20% growth reduction in most of the treated plots. In the amidochlor-treated plots, growth suppression was effective for 3 to 4 weeks at low to medium rates ($0.60mL{\cdot}m^{-2}$). A Type II growth regulator, trinexapac-ethyl exceeding the medium rate of $0.08mL{\cdot}m^{-2}$ consistently tended to suppress vertical shoot growth for 8 weeks, being above 35% reduction in both experiments. In the plots applied with mefluidide, growth suppression appeared with foliar discoloration 3 or 4 days earlier than the other growth regulators and continued to work till the 8 weeks after treatment. Suppression intensity on vertical shoot growth increased with time after treatment up to a certain period of time, depending on growth regulators. Generally, the higher the application rate, the greater the suppression intensity. Seasonal variation of activity and effectiveness of growth regulators was observed, resulting in lower suppression intensity in July than in June. It is expected to reduce mowing requirements by 30 to up to 60% for a certain period with a specific growth regulator. In low to medium maintenance of Korean lawngrass turf, a long-term suppression may be more effectively accomplished with trinexapac-ethyl rather than mefluidide and amidochlor in terms of vertical shoot growth inhibition. Therefore, turf managers will need to select proper growth regulator and determine optimum rate of application for turfgrass management, based on a defined period of mowing reduction.

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Salt Tolerance of Vigna angularis during Germination and Early Seedling Growth

  • Lee, Hee-Kyung;Hong, Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.4 no.1
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    • pp.59-69
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    • 2000
  • The present study was undertaken in investigate the response to salinity and effect of plant growth regulators and proline under salinity stress on the germination and seedling growth of Vigna angularis. The protective effect of external Ca2+ on root elongation under saline conditions was also investigated. The seed germination of Vigna angularis decreased with an increase in salinity. The growth regulators GA3 was more effective than kinetin. At a higher salinity, low concentrations of kinetin and high concentrations of GA3 were more effective. The external application of proline and betaine improved germination under saline conditions. At a low salinity proline and betaine alleviated the salinity-induced inhibition of germination, yet at higher NaCl concentrations, proline and betaine were both ineffective. Exposure to salinity during germination was accompanied by an increase in the proline content, thereby suggesting that one compatible solute in the germinating seed would seem to be proline. The inhibition of germination by high NaCl concentrations was relatively more severe in scarified seeds than in intact seeds, indicating that the seed coat acts as a partial barrier to an Na2+ ameliorated the adverse effect of salinity stress.

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Effect of Plant Growth Regulators on Callus Induction and Plant Regeration of Farfugium japonica (털머위 (Farfugium japonica)의 캘러스 유도 및 식물체 분화에 미치는 생장조절제의 영향)

  • Lee, Seung-Yeob;Yoo, Sung-Oh;Bae, Jong-Hyang;Lee, Joong-Ho
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.45-49
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    • 2002
  • The leaf and petiole segments of Farfugium japonica were cultured to investigate the influence of growth regulators on their callus induction and plant regeneration. The callus induction and growth showed a good response both leaf and petiole on MS media supplemented with 1∼2 mg/L 2,4-D and 1∼2 mg/L BA. Callus induction and growth were more effective in petiole segments than leaf one. The highest percentage of plant regeneration was obtained from 60-day-old calli on MS medium supplemented with 1 mg/L NAA and 2 mg/L BA. When subcultured to the same medium for about 60 days, multiple shoots were developed from regenerating callus. The shoots produced roots after transferring to rooting medium containing 0.5 mg/L IAA. The plantlets over 50 mm in height were successfully acclimatized in vermiculite, and the survival rate was over 95%.