• The Korean Journal of Ecology
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• v.14 no.2
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• pp.149-157
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• 1991

To find out allelopathic potential of naturally occurring substances emitted form pinus korairnsis, water extracts and volatile sudstances of the tree materials werw provided and used for tests such as seed germination and seedling growth test. In general, the more was the concentration of the extracts and essential oils, the worse was growth effects of the selected species. The germination an the growth were inversely proportional to the concentration of the extracts and the essential oils of p. koraiensis. in other words, the chemical substances had the biological toxic activity. gc and gc/ms methods were employed for analysis and identification of phytotoxic substances from the tree leaves. Sixteen chemical compounds were identified from water extracts and nineteen from essential oils of p. koraiensis leaves. Through the experiment on germination and seedling growth test, the chemical substances naturally occurring from p. koraiensis seemed to be responsible for the allelopathic potential in this study.

• The Korean Journal of Mycology
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• v.43 no.4
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• pp.272-276
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• 2015

We examined endophytic fungi from the needle leaves of three species of conifers in Korea: Abies nephrolepis, Thuja koraiensis, Pinus koraiensis. Endophytic fungi were isolated from the surface-sterilized leaves and identified based on morphological characteristics and Internal transcribed spacer sequences of rDNA. Three species of endophytic fungi isolated in this study were the first reported in Korea: Cryptosporiopsis actinidiae, Pestalotiopsis mangiferae, Pyrenochaeta cava.

• Journal of Applied Biological Chemistry
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• v.60 no.1
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• pp.73-78
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• 2017

This study provide activity for beauty food of water and 80 % ethanol extracts from Pinus koraiensis leaves. Total phenolic content of extracts from Pinus koraiensis leaves were each 12.22 mg/g (Drying under hot air) and 17.93 mg/g (Drying under shade), 14.36 mg/g (Lyophilization) in water extracts (WE) and 11.9 mg/g and 20.63 mg/g, 17.96 mg/g in 80 % ethanol extracts (EE). The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity of extracts from Pinus koraiensis leaves was 96.20 % in EE from drying under shade at extracts concentration. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical decolorization activity of extracts from drying under shade was 99.85 % in WE and 99.80 % in EE at extracts concentration. The antioxidant protection factor (PF) extracts from drying under shade type was 9.63 PF in WE and 10.48 PF in EE at extracts concentration. The thiobarbituric acid reactive substance from Pinus koraiensis leaf was 89.39 % in EE from drying under shade at extracts concentration. The elastase inhibition activity of EE for anti-wrinkle effect showed an excellent wrinkle improvement effect, showing 71.46 % in EE from lyophilization. Collagenase inhibition activity of EE from drying under shade was 97.48 % in extracts. Tyrosinase inhibition activity which was related to anti-melanogensis was observed. The tyrosinase inhibitory effect of extracts from lyophilization was confirmed to be 60.4 % in EE more than another drying methods at extracts concentration. Through out all results, it can be expected Pinus koraiensis leaves extracts to use as a functional material for anti-oxidant and functional beauty food.

• Korean Journal of Agricultural Science
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• v.38 no.1
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• pp.11-16
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• 2011

In this study, we report the generation and analysis of a total of 1,211 expressed sequence tags (ESTs) from Pinus koraiensis. A cDNA library was generated from the young leaf tissue and a total of 1,211 cDNA were partially sequenced. EST and unigene sequence quality were determined by computational filtering, manual review, and BLAST analyses. In all, 857 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length 50 nucleotides. A total of 411 unigene, consisting of 89 contigs and 322 singletons, was identified after assembling. Also, we identified 77 new microsatellite-containing sequences from the unigenes and classified the structure according to their repeat unit. According to homology search with BLASTX against the NCBI database, 63.1% of ESTs were homologous with known function and 22.2% of ESTs were matched with putative or unknown function. The remaining 14.6% of ESTs showed no significant similarity to any protein sequences found in the public database. Gene ontology (GO) classification showed that the most abundant GO terms were transport, nucleotide binding, plastid, in terms biological process, molecular function and cellular component, respectively. The sequence data will be used to characterize potential roles of new genes in Pinus and provided for the useful tools as a genetic resource.

• KSBB Journal
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• v.28 no.6
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• pp.380-386
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• 2013

This study was to investigate the chemical composition and biological activities of Pinus Koraiensis seed. The oil, moisture, ash, crude protein, and carbohydrate contents of P. Koraiensis seed were 58.21, 7.84, 1.56, 14.26, and 18.13%, respectively. The ratios of essential amino acid and nonessential amino acid against total amino acids were 36.6 and 60.3%, respectively. The saponification value of seed oil was 166.8 mg KOH/g oil. Among various fatty acids, the linoleic acid content was the highest, 35.5%, which was approximately 72.6% of polyunsaturated fatty acid. The ethylacetate extract of P. Koraiensis seed had the highest DPPH radical scavenging activity (62.8%) at 7.0 mg/mL, followed by hexane extract, methanol extract, and hot water extract. The maximum nitrite scavenging activity was obtained 59.3% at pH 1.2. The total phenolic concentration of ethylacetate extract was 98.7 mg/g, approximately 4.8 folds higher than that of the hot water extract. The maximum inhibition activities of elastase using ethylacetate extract and collagenase using hexane extract were 58.8 and 40.7%, respectively. These results indicate that P. koraiensis seed extract could be applied to present the possibilities of industrial applications for the developments of cosmetics.

• Mycobiology
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• v.50 no.5
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• pp.317-325
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• 2022

Endophytic fungi occupy various ecological niches, which reinforces their diversity. As few studies have investigated the endophytic fungi of alpine conifers, we focused on four species of alpine conifers in this study-Abies nephrolepis, Pinus pumila, Taxus cuspidata var. nana, and Thuja koraiensis-and examined them for endophytic fungi. A total of 108 endophytic fungi were isolated. There were four taxa in A. nephrolepis, 12 in P. pumila, 18 in T. cuspidata var. nana, and 17 in T. koraiensis; these were divided into five classes: Agaricomycetes (3.2%), Dothideomycetes (29.0%), Leotiomycetes (15.0%), Sordariomycetes (41.9%), and Orbiliomycetes (1.6%). The most prevalent fungi were Sydowia polyspora (22.7%) and Xylariaceae sp. (22.7%) in P. pumila, Phomopsis juglandina (16.1%) in T. cuspidate var. nana, and Thuja-endophytes sp. 1 (70.0%) in T. koraiensis. However, there was no dominant species growing in A. nephrolepis. Some host plants were analyzed using next-generation sequencing. We obtained 4618 reads for A. nephrolepis and 2268 reads for T. koraiensis. At the genus level, the top three endophytic fungi were Ophiostomataceae_uc (64.6%), Nectriaceae_uc (15.5%), and unclassified organism (18.0%) in A. nephrolepis and Nectriaceae_uc (41.9%), Ophiostomataceae_uc (41.8%), and Magnaporthaceae_uc (9.2%) in T. koraiensis. Our results show that there are different communities of endophytic fungi among different host plants, even if the host plants are in the same region. Such ecological niches are important in terms of the ecological restoration of alpine conifers.

• Journal of environmental and Sanitary engineering
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• v.5 no.1
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• pp.28-33
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• 1990

The turnover rates of nitrogen(N), phosphorus(P), calcium(Ca), potassium(K), and sodium(Na) during the decomposition of litter were studied in the Pinus koraiensis forest in Choon Chun and in the Pinus rigida forest in Mt. Gwanak, Seoul. The turnover rates of N, P, K, Ca, and Na were 0.064, 0.068, 0.040, 0.417, and 0.058 for the P. korai ensis litter and 0.049, 0.049, 0.023, 0.346, and 0.058 for the P. rigida litter respectively. The loss of elements follows the order Ca>P>N>Na>K in the P. koraiensis litter and the order Ca>Na>N=P>K in the P. rigida litter. Generally the turnover rates of cations were greater than those of anions.

• The Plant Pathology Journal
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• v.16 no.1
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• pp.52-54
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• 2000

Pitch canker of Pinus rigida, caused by Fusarium subglutinans f. sp. pini, was first noticed in Inchon, Korea, and is now being spread into other plantations of pines. Typical symptoms re resin flows from canders on the shoots, branches, and trunks, and resin-soaking of the sap wood under the bark. Pathogenic fungi were isolated from the infected shoots, branches, and sees on Fusarium-selective medium. Mycelial growth and microscopic characteristics were examined. Pathogenicity test was carried out by inoculating four common species of pines (P. rigida, P. densiflora, P. thunbergii, and P. koraiensis) in Korea. P. rigida and P. thunbergii showed symptoms identical to those of naturally infected trees, while P. densiflora and P. koraiensis remained free symptomless.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.1
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• pp.85-95
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• 2008

This study was carried out to analyze Sungkyunkwan University Forest vegetation at Pocheon. Employing the releve method of Braun-Blanquet, 20 plots were sampled. The communities were classified into Larix leptolepis community, Quercus mongolica community, Pinus koraiensis community and Pinus densiflora community. Average coverage rate was 91% in tree layer, 39% in subtree layer, 44% in shrub layer, 43% in herb layer, respectively. The importance values were 61.79% in Larix leptolepis, 52.08% in Quercus mongolica, 44.6% in Pinus koraiensis, 30.35% in Pinus densiflora, 17.78% in Styrax obassia, 10.9% in Fraxinus rhynchophyllia. DBH range of most Quercus mongolica was between 6cm and 15cm. Therefore, Quercus mongolica will be dominant species in the study area for several more decades.

• Journal of the Korean Wood Science and Technology
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• v.12 no.4
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• pp.36-46
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• 1984

In order to investigate the inhibitory index (I) and the effects of hot water extraction treatments and addition of accelerators on the index in hardening of Korean lignocellulosics, portland cement (Type I) and water system, hydration tests were carried out on 8 Korean lignocellulosics, namely, Pinus densiflora, Pinus rigida, Pinus koraiensis, Abies holophylla, Larix leptoiepis, Populus alba-glandulosa, rice husk and rice stalk with or without hot water extraction or chemical additives. The inhibitory index of Pinus densiflora and Pinus rigida found to be suitable under limited conditions for composite without any treatment. With hot water treatment rice husk, Pinus koraiensis, Larix leptolepis, and A hies holophylla were reclassified from not suitable to suitable under limited conditions. Combining hot water extraction with chemical addition of accelerator, calcium chloride of magnesium chloride, Populus alba-glandulosa, Larix leptolepis, and Pinus rigida became highly suitable.