• 디지털융복합연구
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• 제19권1호
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• pp.315-325
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• 2021

소셜 앱에 게이미피케이션 방식이 적용되면서 유저들의 흥미와 제품에 대한 몰입을 보다 더 유발시키며, 그 가치와 중요성이 커지고 있다. 게이미피케이션이란 게임이 아닌 분야에 게임의 기법과 사고를 적용하여 사용자의 몰입을 유도하는 것을 의미한다. 이에 본 논문은 게이미피케이션 분석 모델인 옥탈리시스를 차용하여 중국 3대 소셜 앱에 대한 사용자의 지속 활용도를 분석하여 그 특성을 도출하는 것을 목적으로 한다. 이를 위한 연구의 전개는 첫째, 게이미피케이션의 구성요소와 특성에 대해 기존 사례를 통해 살펴본다. 둘째, 중국 3대 소셜 앱인 위쳇, QQ, 샤오홍슈에 대해 옥탈리시스의 8가지 분석 툴을 통해 설문조사를 실시하고 그 지속 활용도에 대해 분석한다. 분석 결과 게임 메커니즘의 외적 표현인 세 가지 요소 포인트(Point), 배지(Badge), 리더보드(Leadboard)는 사용자의 활용도가 지속되지 않았으며, 이는 단지 하나의 수단일 뿐임을 알 수 있었다. 반면, 소셜 앱의 특성과 콘텐츠 그리고 사용자의 욕구가 결합되어야 게임화 된 사고를 극대화 할 수 있으며, 이에 따라 사용자의 지속 활용도가 유지 됨을 알 수 있다.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.207-216
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• 2021

Supplement of high-protein food plays an important role in improving the symptoms of malnutrition and the immune capacity of the body, but the association of high-protein diet and gut microbiota remained unaddressed. Here, we systematically analyzed the internal organs and gut microbiota in C57(WT) or PD-1H-depleted (KO) mice (T cells were activated) fed with pupae or feed for six weeks. We observed that the body weight gain in the mice fed with pupae increased less significantly than that of the feed group, while the villi and small intestine lengths in the pupa group were reduced compared with that of mice given feed. However, the average body weight of the KO mice increased compared with that of the WT mice fed with pupae or feed. Pupae increased the concentration of blood glucose in WT, but not in KO mice. Moreover, in the feed group, there was no difference in the weight of the internal organs between the WT and KO mice, but in the pupae-fed group, liver weight was decreased and spleen weight was increased compared with that of KO mice. The amounts/plural/amounts of Melainabacteria, Chloroflexi, and Armatimonadetes were specifically upregulated by pupae, and this upregulation was weakened or eliminated by PD-1H depletion. Some bacteria with high abundance in the feed-fed KO mice, such as Deferribacteres, Melainabacteria, Acidobacteria, Bacteroidetes, Spirochaetes and Verrucomicrobia, were decreased in pupae-fed KO mice, and Proteobacteria and Deinococcus were specifically enriched in pupae-fed KO mice. Bacteroidetes, Firmicutes and Akkermansia were associated with weight loss in the pupae-fed group while Lachnospiraceae and Anaerobiospirillum were related glucose metabolism and energy consumption. Based on high-throughput sequencing, we discovered that some gut bacteria specifically regulated the metabolism of a high-protein diet, and PD-1H deficiency improved life quality and sustained blood glucose. Moreover, PD-1H responses to high-protein diet through modulating the type and quantity of gut bacteria. These findings provide evidence about the association among gut microbiota, T cell activation (for PD-1H depletion) and high-protein diet metabolism, have important theoretical significance for nutrition and health research.

• Journal of Microbiology and Biotechnology
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• 제32권3호
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• pp.294-301
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• 2022

In our greenhouse experiment, soil heat treatment groups (50, 80, and 121℃) significantly promoted growth and disease suppression of Panax notoginseng in consecutively cultivated soil (CCS) samples (p < 0.01), and 80℃ worked better than 50℃ and 121℃ (p < 0.01). Furthermore, we found that heat treatment at 80℃ changes the microbial diversity in CCS, and the inhibition ratios of culturable microorganisms, such as fungi and actinomycetes, were nearly 100%. However, the heat-tolerant bacterial community was preserved. The 16S rRNA gene and internal transcribed spacer (ITS) sequencing analyses indicated that the soil heat treatment had a greater effect on the Chao1 index and Shannon's diversity index of bacteria than fungi, and the relative abundances of Firmicutes and Proteobacteria were significantly higher than without heating (80 and 121℃, p < 0.05). Soil probiotic bacteria, such as Bacillus (67%), Sporosarcina (9%), Paenibacillus (6%), Paenisporosarcina (6%), and Cohnella (4%), remained in the soil after the 80℃ and 121℃ heat treatments. Although steam increased the relative abundances of most of the heat-tolerant microbes before sowing, richness and diversity gradually recovered to the level of CCS, regardless of fungi or bacteria, after replanting. Thus, we added heat-tolerant microbes (such as Bacillus) after steaming, which reduced the relative abundance of pathogens, recruited antagonistic bacteria, and provided a long-term protective effect compared to the steaming and Bacillus alone (p < 0.05). Taken together, the current study provides novel insight into sustainable agriculture in a consecutively cultivated system.

• Nuclear Engineering and Technology
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• 제54권7호
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• pp.2728-2735
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• 2022

The research on the flow and heat transfer characteristics of lead bismuth(LBE) is significant for the thermal-hydraulic calculation, safety analysis and practical application of lead-based fast reactors(LFR). In this paper, a new CFD model is proposed to solve the thermal-hydraulic analysis of LBE. The model includes two parts: turbulent model and turbulent Prandtl, which are the important factors for LBE. In order to find the best model, the experiment data and design of 19-pin hexagonal rod bundle with spacer grid, undertaken at the Karlsruhe Liquid Metal Laboratory (KALLA) are used for CFD calculation. Furthermore, the turbulent model includes SST k - 𝜔 and k - 𝜀; the turbulent Prandtl includes Cheng-Tak and constant (Pr_t =1.5,2.0,2.5,3.0). Among them, the combination between SST k - 𝜔 and Cheng-Tak is more suitable for the experiment. But in the low Pe region, the deviation between the experiment data and CFD result is too much. The reason may be the inlet-effect and when Pe is in a low level, the number of molecular thermal diffusion occupies an absolute advantage, and the buoyancy will enhance. In order to test and verify versatility of the model, the NCCL performed by the Nuclear Thermal-hydraulic Laboratory (Nuthel) of Xi'an Jiao tong University is used for CFD to calculate. This paper provides two verification examples for the new universal model.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.52-52
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• 2001

Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic reaction follows a ping-pong mechanism in which the enzyme is transiently phosphorylated on a histidine residue conserved in all nucleoside diphosphate kinases. Beside their role in nucleotide synthesis, these enzymes present additional functions, possibly independent of catalysis, in processes such as differentiation, cell growth, tumor progression, metastasis and development. To clone murine nm23-M5, several expressed sequence tags (ESTs) of the GenBank data base, selected according to their homology to nm23-H5 cDNA, reconstituted a complete open reading frame (GenBank AF222750). To test whether murine NDPKs (1, 2, 3, 4, 5, and 6) can inhibit Bax-mediated toxicity in yeast, co-transformation was performed respectively. The yeast S.cerevisiae was transformed with a copy expression plasmid containing the histidine selection marker and expressing murine Bax under the control of a galactose-inducible promoter. Several clones were selected and found to be growth inhibited when Bax expression was induced with galactose. A representative clone was transformed again with a copy expression plasmid containing the tryptophane selection marker and expressing either murine Bcl-xL or NDPK under the control of a galactose-inducible promoter. Several subclones of the double-transformants were selected and characterized. The ability of Bcl-xL and NDPKs to suppress Bax-mediated toxicity was determined by growing yeast cells overnight in galactose media and spot-testing on galactose plates starting with an equal number of yeast cells as determined by taking the OD$_{600}$. Ten-fold serial dilutions were used in the spot-test. Plates were grown at 3$0^{\circ}C$ for 2-3 days. All murine NDPKs suppressed Bax dependent apoptosis. Futher study will be peformed whether Bax-toxicity inhibition was caused by NDP kinase activity or additional function.n.

• Journal of Ginseng Research
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• 제47권2호
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• pp.199-209
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• 2023

Background: Due to the interrupted blood supply in cerebral ischemic stroke (CIS), ischemic and hypoxia results in neuronal depolarization, insufficient NAD+, excessive levels of ROS, mitochondrial damages, and energy metabolism disorders, which triggers the ischemic cascades. Currently, improvement of mitochondrial functions and energy metabolism is as a vital therapeutic target and clinical strategy. Hence, it is greatly crucial to look for neuroprotective natural agents with mitochondria protection actions and explore the mediated targets for treating CIS. In the previous study, notoginseng leaf triterpenes (PNGL) from Panax notoginseng stems and leaves was demonstrated to have neuroprotective effects against cerebral ischemia/reperfusion injury. However, the potential mechanisms have been not completely elaborate. Methods: The model of middle cerebral artery occlusion and reperfusion (MCAO/R) was adopted to verify the neuroprotective effects and potential pharmacology mechanisms of PNGL in vivo. Antioxidant markers were evaluated by kit detection. Mitochondrial function was evaluated by ATP content measurement, ATPase, NAD and NADH kits. And the transmission electron microscopy (TEM) and pathological staining (H&E and Nissl) were used to detect cerebral morphological changes and mitochondrial structural damages. Western blotting, ELISA and immunofluorescence assay were utilized to explore the mitochondrial protection effects and its related mechanisms in vivo. Results: In vivo, treatment with PNGL markedly reduced excessive oxidative stress, inhibited mitochondrial injury, alleviated energy metabolism dysfunction, decreased neuronal loss and apoptosis, and thus notedly raised neuronal survival under ischemia and hypoxia. Meanwhile, PNGL significantly increased the expression of nicotinamide phosphoribosyltransferase (NAMPT) in the ischemic regions, and regulated its related downstream SIRT1/2/3-MnSOD/PGC-1α pathways. Conclusion: The study finds that the mitochondrial protective effects of PNGL are associated with the NAMPT-SIRT1/2/3-MnSOD/PGC-1α signal pathways. PNGL, as a novel candidate drug, has great application prospects for preventing and treating ischemic stroke.

• Journal of Korean Neurosurgical Society
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• 제66권3호
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• pp.332-339
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• 2023

Objective : The present study aimed to investigate the clinical characteristics of electrolyte imbalance in patients with moderate to severe traumatic brain injury (TBI) who underwent craniotomy and its influence on prognosis. Methods : A total of 156 patients with moderate to severe TBI were prospectively collected from June 2019 to June 2021. All patients underwent craniotomy and intracranial pressure (ICP) monitoring. We aimed to explore the clinical characteristics of electrolyte disturbance and to analyze the influence of electrolyte disturbance on prognosis. Results : A total of 156 patients with moderate and severe TBI were included. There were 57 cases of hypernatremia, accounting for 36.538%, with the average level of 155.788±7.686 mmol/L, which occurred 2.2±0.3 days after injury. There were 25 cases of hyponatremia, accounting for 16.026%, with the average level of 131.204±3.708 mmol/L, which occurred 10.2±3.3 days after injury. There were three cases of hyperkalemia, accounting for 1.923%, with the average level of 7.140±1.297 mmol/L, which occurred 5.3±0.2 days after injury. There were 75 cases of hypokalemia, accounting for 48.077%, with the average level of 3.071±0.302 mmol/L, which occurred 1.8±0.6 days after injury. There were 105 cases of hypocalcemia, accounting for 67.308%, with the average level of 1.846±0.104 mmol/L, which occurred 1.6±0.2 days after injury. There were 17 cases of hypermagnesemia, accounting for 10.897%, with the average level of 1.213±0.426 mmol/L, which occurred 1.8±0.5 days after injury. There were 99 cases of hypomagnesemia, accounting for 63.462%, with the average level of 0.652±0.061 mmol/L, which occurred 1.3±0.4 days after injury. Univariate regression analysis revealed that age, Glasgow coma scale (GCS) score at admission, pupil changes, ICP, hypernatremia, hypocalcemia, hypernatremia combined with hypocalcemia, epilepsy, cerebral infarction, severe hypoproteinemia were statistically abnormal (p<0.05), while gender, hyponatremia, potassium, magnesium, intracranial infection, pneumonia, allogeneic blood transfusion, hypertension, diabetes, abnormal liver function, and abnormal renal function were not statistically significant (p>0.05). After adjusting gender, age, GCS, pupil changes, ICP, epilepsy, cerebral infarction, severe hypoproteinemia, multivariate logistic regression analysis revealed that hypernatremia or hypocalcemia was not statistically significant, while hypernatremia combined with hypocalcemia was statistically significant (p<0.05). Conclusion : The incidence of hypocalcemia was the highest, followed by hypomagnesemia, hypokalemia, hypernatremia, hyponatremia and hypermagnesemia. Hypocalcemia, hypomagnesemia, and hypokalemia generally occurred in the early post-TBI period, hypernatremia occurred in the peak period of ICP, and hyponatremia mostly occurred in the late period after decreased ICP. Hypernatremia combined with hypocalcemia was associated with prognosis.

• 한국엔터테인먼트산업학회논문지
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• 제13권3호
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• pp.231-248
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• 2019

본 연구는 인지자극 활동프로그램을 적용하여 일상생활 및 지역사회 활동은 가능하지만, 지속적인 인지기능의 저하를 겪고 있는 노인의 인지기능, 자기효능감, 우울에 미치는 영향을 알아보고자 하였다. 본 연구는 일상생활 및 지역사회 활동은 가능하지만, 지속적인 인지기능의 저하를 겪고 있는 노인의 특성 및 인지수준에 따른 하위영역별, 난이도별의 다양한 활동을 통해 활동과제에 대한 수행력과 적응력을 증진시킴으로써 인지기능의 하위영역을 체계적으로 향상시키어 치매로의 전환을 예방하고자 하였다. 뿐만 아니라 감각자극을 촉진하는 활동과 상황적·관계적으로 의미가 있는 사건 또는 추억에 대해 자신의 경험이나 생각을 성공적으로 표현함으로써 스스로의 능력에 대한 긍정적인 인식 및 태도를 갖게 하여 자기효능감을 높였다. 그룹활동을 통해서는 자신의 생각과 감정을 표현함은 물론 타인에 대한 공감, 이해, 배려 등을 나눔으로써 우울을 감소시켰다. 따라서 본 연구의 인지자극 활동프로그램은 인지 기능의 저하를 겪고 있는 노인의 임상적 중재로서의 가능성을 확인하였기에, 조기치매의 예방적 차원에서 활용 가능한 프로그램으로 제안하는 바이다.

• 공연문화연구
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• 제19호
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• pp.317-346
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• 2009

숭의묘는 유비를 주향으로 하고 관우와 장비를 배향한 것 외에도 제갈량·조운·마초·황충·왕보·주창·조루·관평 이상 8인을 종향한 사당이다. 배향 인물 중 관우가 포함되어 있어 관왕묘의 하나로 인식되고 논의되었다. 이는 기존에 있던 관왕묘인 동묘, 남묘, 북묘에 이어 '서묘(西廟)'라고도 불린 명칭에서도 확인된다. 물론 관우라는 인물을 모셨다는 공통분모 때문에 관왕묘와 숭의묘의 관련성을 배제할 수는 없다. 그렇지만 숭의묘는 관왕묘와 차별되는 면모가 있다. 숭의묘는 배향인물의 측면에서 관왕묘보다 격이 높았고 아악·아악악현·육일무가 수반되어 사용되는 악무(樂舞)의 종류가 현격히 달랐다. 러일전쟁 발발 직후 1904년 4월 27일에 첫 봉안의식이 행해진 후 1908년 7월에 폐지되기까지 숭의묘제례가 행해진 기간은 불과 4년 밖에 안 되었기 때문에 비교적 덜 알려져 있었다. 게다가 숭의묘 터를 조선총독부에서 고아원과 맹아원으로 사용해 버리는 바람에 그 묘역까지 철저히 훼손되어 숭의묘의 기억을 되돌릴 공간마저 잃어버렸다. 그러나 숭의묘제례는 조선의 전통적인 제례용 악무(樂舞)의 형태를 두루 갖추어 중사(中祀)격으로 행례된 중요한 국가제례의 하나였다. 또한 고종이 황제로 즉위한 후 대한제국의 군사력을 강화하려는 강병책의 일환으로 구상된 제례로, 칭제(稱帝) 이후 환구제례와 더불어 새롭게 만들어졌으며, 대한제국 최후의 국가사전이라는 측면에서 큰 의의가 있다.

• 문화기술의 융합
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• 제9권1호
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• pp.161-168
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• 2023

최근 중국과 대만관계가 위기를 맞으며 미중관계 및 동북아 지역 구도도 크게 흔들리고 있기에 우리는 양안관계의 변화에 주목해야 한다. 이 연구는 제20차 당대회의 '정치 보고'와 지도부 인사에 대한 내용 분석을 중심으로 시진핑의 대만정책을 분석함으로써 양안관계가 당대회 이후 어떻게 변화할 것인지 전망하고자 한다. 연구결과는 다음과같다. 첫째, 당대회 폐막 후 발표한 당헌 수정안에 무력침공의 가능성을 명기했듯이, 시진핑은 통일에 방점을 둔 강경한 대만정책을 펼칠 것이다. 둘째, '정치 보고'의 제2장, 11장, 13장, 14장 내용과 외교·안보 지도부 인사를 분석할 때 향후 미국과의 전략적 경쟁은 계속 심화될 것이다. 이에 따라 양안관계도 상당 기간 불안정성을 보일 것으로 예상된다. 셋째, 대만이 '일국양제'라는 통일방안을 거부하고 탈중국화 경향이 강화되고 있는 상황에서 앞으로 기존의 '반국가분열법'을 세부적으로 강화하는 법제화나 구체적인 시행령이 제정될 가능성이 있다. 넷째, 대만문제에 간섭하는 외부세력과 대만독립 세력에게는 강경한 대응을 할 것이고, 통일에 우호적인 국민당과 대만인들에게는 유인책을 구사하는 등 강온 양면전략이 함께 구사될 것으로 전망된다.