• 제목/요약/키워드: Pine Needles

검색결과 208건 처리시간 0.032초

Pine Needle Extract Applicable to Topical Treatment for the Prevention of Human Papillomavirus Infection

  • Lee, Hee-Jung;Park, Mina;Choi, HeeJae;Nowakowska, Aleksandra;Moon, Chiung;Kwak, Jong Hwan;Kim, Young Bong
    • Journal of Microbiology and Biotechnology
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    • 제31권1호
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    • pp.137-143
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    • 2021
  • Most cervical cancers are associated with high-risk human papillomavirus (HPV) infection. Currently, cervical cancer treatment entails surgical removal of the lesion, but treatment of infection and preventing tissue damage are issues that still remain to be addressed. Herbal medicine and biological studies have focused on developing antiviral drugs from natural sources. In this study, we analyzed the potential antiviral effects of Pinus densiflora Sieb. et Zucc. leaf extracts against HPV. The pine needle extracts from each organic solvent were analyzed for antiviral activity. The methylene chloride fraction (PN-MC) showed the highest activity against HPV pseudovirus (PV). The PN-MC extract was more effective before, rather than after treatment, and therefore represents a prophylactic intervention. Mice were pre-treated with PN-MC via genital application or oral administration, followed by a genital or subcutaneous challenge with HPV PV, respectively. The HPV challenge results showed that mice treated via genital application exhibited complete protection against HPV. In conclusion, PN-MC represents a potential topical virucide for HPV infection.

솔잎 추출물의 in vitro계 암세포 성장억제효과 (Cytotoxic Effect of the Pine needle extracts)

  • 김은정;정성원;최근표;함승시;강하영
    • 한국식품과학회지
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    • 제30권1호
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    • pp.213-217
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    • 1998
  • 곰솔, 리기다, 잣나무, 적송잎의 에탄올 추출물들은 농도가 증가함에 따라 폐암, 간암, 위암, 유방암 세포에 대한 성장억제율이 증가함을 보여 주였고 각각의 디에틸에테르, 클로로포름, 에틸 아세테이트, 부탄올 및 수층 분획물 또한 농도 증가에 따라 성장 억제율이 증가하였다. 그러나 0.25 mg/mL 처리시 각각의 비교 결과 잣나무의 디에틸에테르 분획물은 위암세포에 대해서는 억제효과가 보여지지 않았다. 현미경의 관찰하에서 암세포의 변화는 세포막의 경계가 흐트러지는 사멸 현상을 보였다.

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iNOS 발현 검출을 위한 in vitro 시스템의 확립 및 적송잎 추출물에 의한 저해효과 검증 (Establishment of In vitro Detection System for iNOS Expression and the Verification of Suppressive Effect by Pine Needle Extract)

  • 김남영;장혜지;이동근;장민경;이승우;전명제;김미향;김성구;이상현
    • KSBB Journal
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    • 제26권2호
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    • pp.172-176
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    • 2011
  • This study was aimed to verify suppressive effect of pine-needle extract on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression. In order to evaluate suppressive effect on iNOS expression, RAW 264.7 cells were stably transfected using an iNOS promoterluciferase reporter plasmid yielding RAW 264.7/pGL2-NeomiNOS_ pro11 cells. Established in vitro detection system revealed to diminish LPS-induced iNOS expression by 0.1~500 ${\mu}g/mL$ of saponin at the concentration-dependant manner. Pine needle extract also diminished LPS-induced iNOS expression to 92 and 88% at 500 and 50 ${\mu}g/mL$, respectively. These results suggest that the in vitro detection system developed here could be useful for the verification of suppressive materials on iNOS expression and pine needle extract could be used for the development of functional foods.

조선시대(朝鮮時代) 술에 관한 분석적(分析的) 고찰(考察) -조선중기(朝鮮中期) 1600 년대(年代)를 중심(中心)으로- (A Study on Wine of Yi Dynasty in 1600)

  • 최종희;이효지
    • 한국식생활문화학회지
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    • 제2권1호
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    • pp.17-24
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    • 1987
  • As people know how to brew a wine from fruits and cereals, they continued to develope various wines good to their taste. Korean wines are also ones made from cereals and they have long been eager to improve the delicate taste. They used to drink Takju, raw rice wine, made from nonglutinous rice and Nuruk, a kind of yeast starter. During Koryo Dynasty, Soju a liquor was imported from Won(the Chinese dynasty). Nowadays this traditional folk wine, which had been developed variously and drunk all over the country, is decreasing year after year. The purpose of this study was to review on the wines ; its kinds, raw materials, brewing method, manufacturing utensils, measuring units and devices and the terms for wine making based on 20 documents published in 1600, in the middle of Yi dynesty. The results of review were as follows. 1. There were 121 kinds of wines at that time in Korea. 2. Among the raw materials for wines, major materials were glutinous rice, nonglutinous rice, wheat flour, wheat, mung bean, and black soybean. And minor materials were pepper corn, Lycium chinenisis, cinnamon, pine needles, pine nuts, jujube, mugwort leaves, lotus leaves, pine corn, pine bud, chrysanthemum, pine flowers, honey, Acanthopanox seoultenses, bamboo-root, marrowbone of blak cow, sweet flag, Ciprus noblis, Saurea lappa, honey suckle, Tricho santhes, azalea, the leaves of the paper mulberry, and bark of chungum tree. 3. There were several kinds of wines such as a wine without using Nuruk, a wine made from glutinous rice, nonglutinous rice, or glutinous and nonglutinous rice with flour. 4. There were several brewing methods for wines such as a wine boiled with ring rice cake, a wine brewed with loaves of rice cake, a wine brewed with hard boiled rice, a wine brewed with rice gruel, and a wine brewed with powdered rice gruel. 5. There were 23 kinds of utensils including measuring devices for weight and volume.

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구미 불산 누출사고로 인한 주변지역 환경영향권 설정에 관한 연구 (Defining Area of Damage of 2012 Hydrofluoric Acid Spill Accident in Gumi, Korea)

  • 고도현;김정수;최경호
    • 한국환경보건학회지
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    • 제40권1호
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    • pp.27-37
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    • 2014
  • Objectives: On September 27, 2012, leakage of anhydrous hydrofluoric acid occurred in a chemical plant in the Gumi National Industrial Complex. Following the accident, local factory workers and residents complained of abnormal health conditions. In addition, visual discolorations were widely observed in crops and trees in surrounding areas. The main objectives of the present study were to identify the area that was affected by the spill using data obtained from plants, soil, and water samples after the accident. Methods: Fluoride concentrations were analyzed in pine tree needles, soil, nearby streams, ponds and reservoirs collected from an area within a radius of three kilometers from the plant where the leak occurred. Fluoride concentrations in the air at the time of leakage were then estimated from fluoride concentrations that were measured in the pine tree needles. A Kriged map was developed to describe the spatial distribution of hydrofluoric acid at the time of the leakage and was compared with the area designated as a Special Disaster Zone by the government. Results: The Special Disaster Zone did not include all the affected area that was estimated by the Kriged map. Analytical results of the environmental samples also supported this discrepancy. Conclusion: Using plants, atmospheric concentrations of fluoride at the time of the leakage could be estimated. For the area that was identified as affected, further public health risk assessment and environmental risk assessment should be considered. Also, in the absence of air monitoring at the time of leakage, studies employing plants may be conducted in order to better understand the spatial extent and severity of the contamination.

솔잎에서 분리된 항산화 물질인 4-hydroxy-5methyl-3[2H]-furanone의 멜라닌 생성 억제작용 (Anti-melanogenesis effect of 4-hydroxy-5-methyl-3[2H]-furanone, an antioxidant isolated from pine needles)

  • 부용출;전체옥
    • 대한화장품학회지
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    • 제20권1호
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    • pp.1-13
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    • 1994
  • 솔잎으로부터 프리 라디칼 소거 작용이 있는 물질을 분리하고, 여러 기기 분석 결과에 근거하여 4-hydroxy-5-methyl-3[2H]-furanone (HMF) 으로 동정하였다. 이 물질이 1, 1-diphenyl-2-picrylhydrazyl 프리 라디칼에 대한 소거 작용이 공지의 항산화 물질인 a-tocopherol, ascorbic acid와 유사하였다. HMF는 흰쥐 간 microsome분획에서 Fe(II)/ascorbate에 의해 유도된 지질 과산화를 억제하였으며, 배양 fibroblast 세포에서 자외선에 대한 보호효과를 나타내었다. 이 물질은 또한 tyrosine의 효소적 산화와 Dopa의 자동 산화를 억제하였을 뿐만 아니라 배양 murine melanoma 세포에서도 강력한 멜라닌 생성 억제 작용을 보였다. 피부 세포에서의 멜라닌 생성이 산화적 스트레스에 의해 유발되고 또 효소, 비효소적인 산화 반응을 통해 진행된다고 볼 때, HMF는 이러한 각 단계에서 항산화제로 작용하여 궁극적으로 세포에서의 멜라닌 생성을 막는 것으로 추론되었다.

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방위(方位) 및 수관부위별(樹冠部位別)에 의한 해송 침엽(針葉) 수지구수(樹脂溝數)의 변이(變異) (Variation of Resin Canal Numbers of Black Pine (Pinus thunbergii Parl.) Needles by Tree age and Topophysis of Crown)

  • 이강영;임경빈
    • 한국산림과학회지
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    • 제22권1호
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    • pp.67-70
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    • 1974
  • 수령별(樹齡別) 수관(樹冠)에 착생(着生)된 부위(部位)와 방법(方法)에 따른 해송 침엽(針葉)의 수지구수(樹脂溝數)를 알고자 실험(實驗)을 수행(遂行)하였든바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 수령별(樹齡別) 평균(平均) 수지구수(樹脂溝數)는 5년생이후(年生以後)에서는 증가(增加)되고 10년생이후(年生以後) 부터는 차이(差異)를 보이지 않는다. 2. 엽장(葉長)의 수지구수(樹脂溝數)는 2~9의 Range를 보였고 그 이상(以上)은 발견(發見)되지 못하였다. 3. 방위(方位) 및 수관(樹冠) 부위별(部位別)에 의한 수지구수(樹脂溝數)를 분산분석(分散分析)한 결과(結果) 유의차(有意差)는 없었으며 5년생(年生)의 것은 개체간(個體間)에 유의성(有意性)을 보였으며 10년생(年生)은 낮은 유의성(有意性)을 보였다. 이상(以上)의 결과(結果)로 보아 해송 침엽(針葉)의 수지구수(樹脂溝數)는 방위(方位) 및 착생부위(着生部位)에 의한 차이(差異)는 없는 것으로 추정(推定)된다.

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Adsorption characteristics of NH4-N by biochar derived from pine needles

  • Kang, Yun-Gu;Lee, Jun-Young;Chun, Jin-Hyuk;Lee, Jae-Han;Yun, Yeo-Uk;Oh, Taek-Keun
    • 농업과학연구
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    • 제48권3호
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    • pp.589-596
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    • 2021
  • Nitrogen applied to soil is highly prone to leaching and volatilization leading to gaseous emissions of nitrous oxide (N2O) and ammonia (NH3) which are of great environmental concern. Usage of biochar to reduce the discharge of nitrogen to the environment has attracted much interest in the recent past. Biochar is produced by pyrolyzing various biomasses under oxygen-limited conditions. Biochar is a carbonized material with high adsorptive powers for not only plant nutrients but also heavy metals. The objective of this study was to investigate the adsorption characteristics of NH4-N onto biochar made from pine needles. The biochar was produced at various pyrolysis temperatures including 300, 400 and 500℃ and holding times of 30 and 120 minutes. The Langmuir isotherm was used to evaluate the adsorption test results. The chemical properties of the biochar varied with the pyrolysis conditions. In particular, the pH, EC and total carbon content increased with the increasing pyrolysis conditions. The rate of adsorption of NH4-N by the biochar decreased with the increasing pyrolysis conditions. Of these conditions, biochar that was pyrolyzed at 300℃ for 30 minutes showed the highest adsorption rate of approximately 0.071 mg·g-1. Thus, the use of biochar pyrolyzed at low temperatures with a short holding time can most efficiently reduce ammonia emissions from agricultural land.

Pinus thunbergii×P. massoniana F1 교잡종(交雜種) 유묘(幼苗)의 특성(特性) (Characteristics of Pinus thunbergii×P. massoniana F1 Hybrid Seedlings)

  • 박문한;전계상;윤양
    • 한국산림과학회지
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    • 제69권1호
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    • pp.51-55
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    • 1985
  • Pinus thunbergii를 교배모수(交配母樹)로 하고 대만(台灣)으로부터 도입(導入)된 P. massoniana 화분(花粉)으로 인공교배(人工交配)한 교잡종(交雜種)과 그의 모수(母樹) 풍매차대(風媒次代)들에 대(對)한 충실종자생산(充實種子生産), 생장(生長), 외부형태학적(外部形態學的) 특성(特性) 및 Phenol 물질함량(物質含量)을 조사(調査)하였다. 1) 구과당(毬果當) 충실종자(充實種子) 생산량(生産量)은 교잡종(交雜種)이 평균(平均) 2.3립(粒)으로 풍매(風媒) 26립(粒)에 비(比)하여 저조(低調)하였다. 2) 유묘시(幼苗時) 묘고생장(苗高生長)은 교잡종(交雜種)이 풍매차대(風媒次代)에 비(比)해 평균(平均) 151%로 우수(優秀)하였으며 120~208%의 변이(變異)를 나타냈다. 3) 침엽(針葉)의 외부형태학적(外部形態學的) 특성(特性)은 교잡종(交雜種)이 풍매차대묘(風媒次代苗)에 비(比)하여 침엽(針葉)이 부드럽고, 연(軟)하고, 길며, 연록색(軟綠色)을 나타내고 해부형태(解剖形態)에 있어서는 수지구(樹脂溝)의 위치(位置)가 P. thunbergii 풍매차대(風媒次代)는 중위(中位)인데 비(比)하여 교잡종(交雜種)들은 중위(中位)와 외위(外位)가 동시(同時)에 나타났다. 4) Phenol 물질분석(物質分析)에 있어서는 교잡종(交雜種)과 P. thunbergii 풍매차대간(風媒次代間)에 뚜렷한 차이(差異)가 없었으나 Unknown 4와 6의 물질(物質)이 교잡종(交雜種)에만 나타난 사실(事實)은 특이(特異)하나 이것이 화분수(花粉樹)에서 유래(由來)된 것인지의 여부(與否)는 좀 더 구명(究明)되어야 할 과제(課題)이다.

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식품보존에 이용된 식물의 천연보존료 함유량 연구 (A study of the levels of natural preservatives in wild plants)

  • 백경아;강현각;신명희;박종진;김종대;박성민;이미영;임지순
    • 한국식품저장유통학회지
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    • 제21권4호
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    • pp.529-535
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    • 2014
  • 본 연구는 전통적으로 방부 또는 살균효과가 있어 떡 등에 첨가되거나 저장 및 포장에 이용되었던 자생식물 15종 21건에 대하여 데하이드로초산(sodium dehydroacetate), 소르빈산(sorbic acid), 안식향산(benzoic acid), 파라옥시안 식향산메틸(methyl p-hydroxybenzoate), 파라옥시안식향산에틸(ethyl p-hydroxybenzoate), 파라옥시안식향산프로필(propyl p-hydroxybenzoate), 파라옥시안식향산이소프로필(isopropyl p-hydroxybenzoate), 파라옥시안식향산이소부틸(isobutyl p-hydroxybenzoate), 파라옥시안식향산부틸(butyl p-hydroxybenzoate), 프로피온산(propionic acid)의 함유량을 분석하였다. 솔잎, 망개잎, 대나무잎 등 15종에 대한 천연유래 보존료 함유량을 조사한 결과 솔잎, 대나무잎, 칡잎, 모시잎, 쑥, 잣나무잎 등 6종에서 안식향산, 소르빈산, 프로피온산을 확인할 수 있었다. 안식향산은 대나무잎, 모시잎, 솔잎, 쑥, 칡잎, 잣나무잎에서 8.201~21.839 mg/kg, 소르빈산은 대나무잎과 칡잎에서 5.630~24.995 mg/kg, 프로피온산은 모시잎에서 61.324~62.726 mg/kg이 검출되었다. 조사 식물 중 망개잎, 토란잎, 조릿대, 연잎, 원추리, 구절초, 떡갈나무잎, 산초잎, 감잎 등 9종에서는 천연보존성분이 검출되지 않았다.