• Title/Summary/Keyword: Pin-by-pin

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Production of Fibrinolytic Enzyme and Peptides from Alkaline Fermentation of Soybean Curd Residue by Bacillus firmus NA-1 (Bacillus firmus NA-1 균주를 이용한 비지로부터 혈전분해능효소 및 펩타이드 생산)

  • Oh, Soo-Myung;Seo, Ji-Hyun;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.6
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    • pp.904-909
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    • 2005
  • To produce functional food ingredient from the soybean curd residue (SCR), alkaline fermentation was performed with SCR from cold processed (D-SCR) or hot processed (P-SCR) tofu. The solid- state fermentation was performed by Bacillus firmus NA-1 at $42^{\circ}C$. The fermentation of heat-treated D- SCR resulted in higher production of peptides and fibrinolytic enzyme compared with D-SCR without heating. The P-SCR showed higher production of peptides, fibrinolytic enzyme, indicating alkaline pH after fermentation for 18 hr. When the moisture content of P-SCR was reduced to $60\%$, the production of peptides and fibrinolytic enzyme were enhanced. The P-SCR fortified with $10\%$ MFS (micronized full-fat soy flour) showed higher fibrinolytic enzyme activity and consistency index by fermentation of Bacillus firmus NA-1 Furthermore, the P- SCR fortified with $20\%$ MFS indicated relatively higher peptide content, fibrinolytic enzyme activity and enhanced flavor. By increasing the addition of MFS, the peptide content of fermented P-SCR was increased significantly, but fibrinolytic enzyme was slightly decreased.

Evaluation for Applying Low-Cost Mobile Laser System to Earth Volume Calculation at Construction Site (건설현장에서의 토공량산출을 위한 저가형 모바일 레이저시스템의 적용성 평가)

  • Kim, In Seop
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.30 no.6_1
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    • pp.539-547
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    • 2012
  • Volume survey for earthwork is conducted periodically at every month at large scaled construction sites for request payment for the construction. Currently, the earthwork volume is calculated on the overlapped drawing for two cross sections which were created by new and previous surveying data observed by GPS and TS. However the survey is required lots of time and expenses as it is a pin-point surveying method thus surveyors intend to get data only for the heavy undulated topographic features chosen by himself to save time and expenses. Consequently, it causes poor results due to low dense measurement as well as it's not matched with the results by another surveyors. As a result, the conflicts related in earthwork volume calculation often arises among the owner, contractors and sub contractors. In this study, we developed a method to get surveying data for fast and accurate volume calculation using 3D Mobile Laser System and conducted an application test. Also we carried out experiments for topographic survey using MLS to judge for the application additionally. The results showed that we could reduce a time for cross section survey from 48 hours to 2 hours and collected high dense data which have 0.2m interval instead of 3m interval compare with existing method. Also we could make a map which has high accuracy within 10cm in horizontal through topographic survey using MLS.

Physicochemical Properties of a Biopolymer Flocculant Produced from Bacillus subtilis PUL-A (Bacillus subtilis PUL-A로부터 생산된 Biopolymer 응집제의 물리화학적 특성)

  • Ryu, Mi-Jin;Jang, Eun-Kyung;Lee, Sam-Pin
    • Microbiology and Biotechnology Letters
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    • v.35 no.3
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    • pp.203-209
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    • 2007
  • Soybean milk cake (SMC) was used for the solid-state fermentation by Bacillus subtilis PUL-A isolated from soybean milk cake. In the presence of 5% glutamate the maximum production of biopolymer (59.9 g/kg) was performed by fermentation at $42^{\circ}C$ for 24 hr. The recovered biopolymer was consisted of 87% $\gamma$-polyglutamic acid with molecular weight of $1.3{\times}10^6$ dalton and other biopolymer. The biopolymer solution showed the great decrease in consistency below pH 6.0, regardless of the molecular weight of PGA. Biopolymer solution has a typical pseudoplastic flow behavior and yield stress. The consistency of biopolymer solution was greatly decreased by increasing heating time and temperature in acidic condition compared to the alkaline condition. In kaolin clay suspension, the flocculating activity of biopolymer was the highest value with 15 mg/L biopolymer and 4.5 mM $CaCl_2$, but decreased greatly with $FeCl_3$. The flocculating activity of biopolymer was maximum at pH5, but decreased drastically by heating at $60{\sim}100^{\circ}C$. In particular, biopolymer with native PGA showed the efficient flocculating activity compared to that of modified biopolymer containing low molecular weight of PGA.

Evaluation of Rheological and Functional Properties of Roasted Soybean Flour and Mixed Cereals Fermented by Bacillus sp. (고초균을 이용한 볶은 콩과 곡류 혼합 발효물의 물성 및 기능성 평가)

  • Son, Se-Jin;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.3
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    • pp.450-457
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    • 2011
  • Roasted soybean flour (RSF) and mixed cereals were fermented by the solid state fermentation using Bacillus subtilis HA to optimize the production of biologically active compounds. The RFS fermented with 52.7% moisture content showed higher production with protease activity of 42.6 unit/g and 10% mucilage content after fermentation for 24 hr. Tyrosine content and protease activity after 48 hr fermentation time were the highest values with 445.5 mg% and 55.1 unit/g, respectively. However, the wholesome fermented RSF (FRSF) was obtained by fermentation for 24 hr because of the production of unpleasant flavors after fermentation for 48 hr. The RSF fermented with various types and contents of cereals has no effects on tyrosine content and protease activity. However, the addition of brown rice significantly increased mucilage content, especially indicating 24.55% at the addition of 80% (w/w). For addition of barley, fibrinolytic activity was increased to 11.82 unit/g by the fortification of 60% barley. It is concluded that biologically active compounds including fibrinolytic activity and mucilage content in FRSF were dependent upon the type and content of various cereals.

Effects of Various Polysaccharides on the Physicochemical Properties of the Dextran Culture Containing Carrot Juice Residue Obtained from Submerged Culture Using Leuconostoc citreum S5 (다당류 첨가에 따른 당근박 함유 Leuconostoc citreum S5 발효물의 물성변화)

  • Son, Min-Jung;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.3
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    • pp.352-358
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    • 2009
  • The physicochemical properties of dextran culture fermented by Leuconostoc citreum S5 were evaluated by the addition of various polysaccharides and heat-treatment. The consistency of dextran culture increased with the addition of carrot juice residue (CJR) in submerged culture, resulting in the highest consistency of 150 $Pa{\cdot}s^n$ and viable cell counts of $2.36{\times}10^9$ CFU/mL at 20% level of CJR. The dextran culture showed the pseudoplastic behavior and its consistency was greatly increased with the addition of various polysaccharides at 2% level. Addition of glucomannan indicated the highest consistency of 1275 $Pa{\cdot}s^n$ and their heat-treatment resulted in the increase of consistency except for glucomannan. After heat-treatment, the fermented dextran culture containing CJR fortified with gellan gum and carrageenan showed great change in rheology, indicating the highest consistency and hardness value resulted in the great increase of elastic and viscous moduli. The dynamic viscoelastic properties of dextran culture were greatly increased by the heat-treatment after fortification of various polysaccharides. Thus, the consistency and viable cell counts of dextran culture were increased with the addition of CJR. The rheological properties of dextran culture were manipulated by the fortification of various polysaccharides and heat-treatment.

Developing an Early Leakage Detection System for Thermal Power Plant Boiler Tubes by Using Acoustic Emission Technology (음향방출법을 이용한 발전용 보일러 튜브 미세누설 조기 탐지 시스템 개발 및 성능 검증)

  • Lee, Sang Bum;Roh, Seon Man
    • Journal of the Korean Society for Nondestructive Testing
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    • v.36 no.3
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    • pp.181-187
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    • 2016
  • A thermal power plant has a heat exchanger tube to collect and convert the heat generated from the high temperature and pressure steam to energy, but the tubes are arranged in a complex manner. In the event that a leakage occurs in any of these tubes, the high-pressure steam leaks out and may cause the neighboring tubes to rupture. This leakage can finally stop power generation, and hence there is a dire need to establish a suitable technology capable of detecting tube leaks at an early stage even before it occurs. As shown in this paper, by applying acoustic emission (AE) technology in existing boiler tube leak detection equipment (BTLD), we developed a system that detects these leakages early enough and generates an alarm at an early stage to necessitate action; the developed system works better that the existing system used to detect fine leakages. We verified the usability of the system in a 560MW-class thermal power plant boiler by conducting leak tests by simulating leakages from a variety of hole sizes (ⵁ2, ⵁ5, ⵁ10 mm). Results show that while the existing fine leakage detection system does not detect fine leakages of ⵁ2 mm and ⵁ5 mm, the newly developed system could detect leakages early enough and generate an alarm at an early stage, and it is possible to increase the signal to more than 18 dB.

Simulation of Vehicle-Structure Dynamic Interaction by Displacement Constraint Equations and Stabilized Penalty Method (변위제한조건식과 안정화된 Penalty방법에 의한 차량 주행에 따른 구조물의 동적상호작용 해석기법)

  • Chung, Keun Young;Lee, Sung Uk;Min, Kyung Ju
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.4D
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    • pp.671-678
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    • 2006
  • In this study, to describe vehicle-structure dynamic interaction phenomena with 1/4 vehicle model, nonlinear Hertzian contact spring and nonlinear contact damper are adopted. The external loads acting on 1/4 vehicle model are selfweight of vehicle and geometry information of running surface. The constraint equation on contact surface is implemented by the Penalty method with stabilization and the reaction from constraint violation. To describe pitching motion of various vehicles two types of the displacement constraint equations are exerted to connect between car bodies and between bogie frames, i.e., the rigid body connection and the rigid body connection with pin, respectively. For the time integration of dynamic equations of vehicles and structure Newmark time integration scheme is adopted. To reduce the error caused by inadequate time step size, adaptive time-stepping technique is also adopted. Thus, it is expected that more versatile dynamic interaction phenomena can be described by this approach and it can be applied to various railway dynamic problems with low computational cost.

Cholesterol Improvement Synergistic Effects of Fermented Soybean Grits Caused by Added with Mung Bean in vitro (녹두 첨가로 인한 탈지대두 Grits(Defatted Soybean Grits) 발효물의 in vitro 상에서의 콜레스테롤 개선능 상승효과)

  • Lee, Sung-Gyu;Kim, Hyun-Jeong;Yu, Mi-Hee;Lee, Eun-Ju;Lee, Sam-Pin;Lee, In-Seon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.7
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    • pp.947-952
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    • 2010
  • This study was performed to investigate cholesterol improvement of fermented defatted soybean grits (FD) and FD added with 2.5, 5, 10% mung bean (FDM). The FD and FDM were prepared by the solid state fermentation using Bacillus subtilis NUC1 at $40^{\circ}C$ for 24 hr. More than 70% cholesterol adsorption of FD and FDM groups was shown. Particularly, FDM added with 2.5% mung bean (2.5% FDM) showed highest cholesterol adsorption by 90% among FD and FDM groups. 2.5% FDM showed 42% inhibition effect on HMG-CoA reductase, and significantly decreased the intracellular cholesterol contents in HepG2 cells. Apolipoprotein AI, CIII improvement effects of FD and FDM group in HepG2 cells showed most effects in the 2.5% FDM. The results suggest that FDM added with 2.5% mung bean may be beneficial to the prevention of hypercholesterol.

Relationship among Plasma Homocysteine, Folate, Vitamin $B_{12}$ and Nutrient Intake and Neurocognitive Function in the Elderly (노인의 혈중 호모시스테인, 엽산, 비타민 $B_{12}$ 수준 및 영양소 섭취 상태와 신경인지기능과의 관련성)

  • Kim, Hee-Jung;Kim, Hye-Sook;Kim, Ki-Nam;Kim, Ggot-Pin;Son, Jung-In;Kim, Seong-Yoon;Chang, Nam-Soo
    • Journal of Nutrition and Health
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    • v.44 no.6
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    • pp.498-506
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    • 2011
  • This study examined the relationship among plasma homocysteine, folate, and vitamin $B_{12}$ levels and neurocognitive function in 118 community-dwelling elderly subjects (mean age, $75.1{\pm}6.7$ years). The Mini-Mental State Examination (MMSE-KC) was used to screen and assess neurocognitive function in the participants. Dietary intake data including the use of dietary supplements were obtained using the 24-hour recall method by well-trained interviewers. Plasma folate and vitamin $B_{12}$ concentrations were analyzed by radioimmunoassay, and homocysteine was assessed by a high performance liquid chromatography-fluorescence method. The proportions of participants with suboptimal levels of plasma folate (< 3 ng/mL), vitamin $B_{12}$ (< 221 pmol/mL), and homocysteine (> $15{\mu}mol/L$) were 16.1%, 5.9%, and 21.2%, respectively. A multiple regression analysis showed that plasma homocysteine was negatively associated with plasma folate and vitamin $B_{12}$ levels. The MMSE-KC test scores were significantly associated with plasma homocysteine and folate, but not with vitamin $B_{12}$, after adjusting for age, gender, body mass index, living with spouse, education, current smoking, energy intake, and chronic diseases such as hypertension, diabetes, thyroid disease, dyslipidemia, stroke, and cardiovascular disease. A general linear model adjusted for covariates revealed that MMSE-KC test scores increased from the lowest to the highest quartiles of vitamin $B_1$, vitamin $B_2$, vitamin $B_6$, vitamin $B_{12}$, and vitamin C intake (p for trend = 0.012, 0.039, 0.014, 0.046, 0.026, respectively). These results indicate that the problem of folate inadequacy and hyperhomocysteinemia are highly prevalent among community-dwelling elderly people and that dietary intake of the B vitamins and vitamin C is positively associated with cognitive function scores.

Physicochemical and Antioxidant Properties of Red Ginseng Marc Fermented by Bacillus subtilis HA with Mugwort Powder Addition (고초균 발효에 의한 홍삼박 발효물에 쑥 분말 첨가에 따른 물리화학적 및 항산화적 특성)

  • Jung, Hye-Won;Kim, Ji-Eun;Seo, Ji-Hyun;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.9
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    • pp.1391-1398
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    • 2010
  • Red ginseng marc (RGM) was fermented by the solid-state fermentation using Bacillus subtilis HA to produce biologically active compounds. The red ginseng marc fermented without mugwort possessed higher mucilage content (11.5%) and proteolytic activity (277.5 unit/g). The RGM fermented with 3% mugwort showed lower production of mucilage and protease activity whereas higher tyrosine content (581.3 mg%) and consistency index ($8.8\;Pa{\cdot}s^n$). The mucilage produced from fermented RGM contained $\gamma$-PGA with 1,100 kDa of molecular weight, and its yield was 15.9 g/kg. 70% ethanol extract from the RGM fermented with 3% mugwort had the highest DPPH radical scavenging effect ($IC_{50}$ value of 0.57 mg/mL), and the water extract showed the highest ABTS radical scavenging effect, indicating $IC_{50}$ value of 1.24 mg/mL. Overall, the RGM fermented by B. subtilis HA with mugwort contained various biologically active compounds having antioxidant effects.