• YAKHAK HOEJI
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• v.41 no.1
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• pp.105-110
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• 1997

Two protein-polysaccharide fractions, GLA and GLB, respectively, were prepared from the pileus of the fully grown carpophores and the tips of the growing carpophores of Ganod erma lucidum. At a dose of 100mg/kg/day ip, GLA and GLB inhibited the growth of sarcoma 180 solid tumor in ICR mice by 56.3% and 81.8%, respectively. In a flow cytometric (FCM) analysis, GLA and GLB enhanced the formation of lymphoblasts of BALB/c, splenic leukocytes at a concentration of 100 ${\mu}$g/ml, by 38.3% and 61, 3%, respectively. When ip injected into ICR mice, GLB exerted anti-leukopenic effect against cyclophospamide (75mg/kg, ip) in that the leukocyte counts of the peripheral blood of the normal and the cyclophosphamide-treated mice. respectively. was (11.1 ${\pm}$ 3.8) ${\times}$ 10$^3$ and (4.0 ${\pm}$ 1.8) ${\times}$ 10$^3$, while the GLB-cyclophosphamide treated mice showed a leukocyte count of (10.8 ${\pm}$ 5.1) ${\times}$ 10$^3$ CELLS/${\mu}$l. These results suggest that GLB is a promising candidate for an effective, cancer immunotherapeutic agent.

• Mycobiology
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• v.39 no.4
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• pp.272-277
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• 2011

Chemical mutagenesis of basidiospores of Hypsizygus marmoreus generated new mushroom strains. The basidospores were treated with methanesulfonate methylester, an alkylating agent, to yield 400 mutant monokaryotic mycelia. Twenty fast-growing mycelia were selected and mated each other by hyphal fusion. Fifty out of the 190 matings were successful (mating rate of 26.3%), judged by the formation of clamp connections. The mutant dikaryons were cultivated to investigate their morphological and cultivation characteristics. Mutant strains No. 3 and No. 5 showed 10% and 6% increase in fruiting body production, respectively. Eight mutant strains showed delayed and reduced primordia formation, resulting in the reduced production yield with prolonged cultivation period. The number of the fruiting bodies of mutant No. 31, which displayed reduced primordial formation, was only 15, compared to the parental number of 65. Another interesting phenotype was a fruiting body with a flattened stipe and pileus. Dikaryons generated by mating with the mutant spore No. 14 produced flat fruiting bodies. Further molecular biological studies will provide details of the mechanism. This work shows that the chemical mutagenesis approach is highly utilizable in the development of mushroom strains as well as in the generation of resources for molecular genetic studies.

• Mycobiology
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• v.43 no.3
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• pp.327-332
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• 2015

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. The translated amino acid sequence of Fvpal shares a high identity (66%) with that of ectomycorrhizal fungus Tricholoma matsutake. Distinctively, the Fvpal expression in the mycelium was higher in minimal medium supplemented with L-tyrosine than with other aromatic amino acids. During cultivation of the mushroom on sawdust medium, Fvpal expression in the fruit body correspondingly increased as the mushroom grew. In the fruiting body, Fvpal was expressed more in the stipe than in the pileus. These results suggest that F. velutipes PAL activity differs in the different organs of the mushroom. Overall, this is first report to show that the PAL gene expression is associated with mushroom growth in fungi.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.25-28
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• 2010

New shiitake(Lentinula edodes) strain "Sanlim No. 7" was produced by Di-mon hybridization method. Bed-log cultivation of Sanlim No. 7 was performed. Fruit-body production was most at autumn and it is suitable to use as fresh shiitake rather than dried one. The fruit body is hemispherical shape, brown coloured and diameter of pileus is ca. 47 mm. Row of gill is straight, and its width is narrow. Optimum temperature of fruit-body formation was $11{\sim}23^{\circ}C$, and the fruiting is sporadic. The total amount of fruit-body production during 4 years(one generation) was ca. 1.4 kg/log.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.197-198
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• 2010

The morphological and ecological characteristics of Russula eburneoareolata Hongo is reported first time in Korea. The fungus was collected from the mixed forest of Fagaceae in the Gyeryongsan Mountain National Park. The pileus is champagne to ivory, convex then expanded and depressed in the center afterward, and 4 to 9 cm broad. Stipe is 3 to 6 cm long, 12 to 20mm thick and tapering below. Spores are $7-8{\times}6-7\;{\mu}m$, subspherical oval and warty with some connecting lines. Basidia are about $35-40{\times}10-11\;{\mu}m$ and four-spore bearing type.

• Journal of Practical Agriculture & Fisheries Research
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• v.13 no.1
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• pp.185-202
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• 2011

As a result of survey the dealings amounts of agricultural products in the public management wholesale market from 1997 to 2009 for 13 years, that makes composted of 54,706 tons in 1997, 130,345 tons in 2003 by increasing 238%, that is resulted of about 20% increase every year. As a result of survey the dealings money of agricultural products in the public management wholesale market from 1997 to 2009 for 13 years, that makes composted of 168,074 million won in 1997, 323,232 million won in 2003 by increasing 192%, that is resulted of about 16% increase every year. It has corelation with dealings amounts and money each other, also appeals to transition without discrimination, but making high amounts than money. It is divided into excellent, good and poor products according to freshness, pileus size, color and stem length to forwarding method field survey in farm produce wholesale market.

• Journal of Mushroom
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• v.2 no.4
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• pp.218-221
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• 2004

Jinmi oyster mushroom(Pleurotus ostreatus) were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2003. This oyster mushroom were bred and cultivated one after mating single spores collected from K3-2 and ASI2018-249. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around $25{\sim}30^{\circ}C$ and that for the pinheading and growth of fruitbody was around $15{\pm}1^{\circ}C$. Around 22 days at $20^{\circ}C$ were required for incubation of Jinmi and the yield was shown high by 145.9g/bottle.

• Journal of Mushroom
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• v.8 no.4
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• pp.157-160
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• 2010

Agaricus blazei Murill is a important medicinal mushroom for a powerful immune system builder and tonic. Currently, it is known about a new disease phenomenon that appears to be occurring on a number of mushroom farms. We described a straightforward approach in which molecular methods was used to survey the presence of potentially endo- and epiphytic bacteria infected with the Agaricus blazei. The 16S rDNA was amplified with universal eubacterial primers directly from pure cultures of Agaricus blazei mycelium and fruit body. The 16S rDNA sequences were almost identical (96 to 97% similarity), and phylogenetic analysis placed them in a single unique rRNA branch belong to the uncultural bacterium phylogroup. PCR detection of uncultural bacterium in the malformed tissues of Agaricus blazei were carried out by using 16S rRNA sequenced specific probe. It was strongly amplified at the malformed pileus region of fruit body and also spore print was impossible.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.94-99
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• 2016

Since development of the first Korean button mushroom cultivar 'Sae-Ah' in 2010, mushroom farmers have demanded an improved cultivar. Although a new cultivar, 'Saejeong', was developed in 2011, this cultivar has thus far been difficult to cultivate in typical mushroom farms. To solve this problem, another new cultivar, 'Saedo', was developed in 2012. This new cultivar was selected by genetic analysis from crossing the heterokaryon line A175 and the homokaryon line ASI1346-15. The heterokaryon A175 was a crossed line between strains ASI1038-211 and ASI1346-20. The mycelium of 'Saedo' showed good growth on compost dextrose agar at $25^{\circ}C$. The morphological traits such as the pileus (cap) and stipe of the 'Saedo' cultivar were thicker than those of the reference control 'Saejeong', and the production yield was increased by 1.89-fold compared to that of the reference control. A field experiment of the 'Saedo' cultivar has been ongoing since 2013. The yield of the 'Saedo' cultivar was found to be substantially higher than that of the reference control during the $2^{nd}$ flush.

• Journal of Mushroom
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• v.4 no.3
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• pp.106-110
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• 2006

A new commercial strain 'Jinhwang' of Pholiota adiposa was developed by hyphal anastomosis. It was developed with crossing between monokaryotic strains derived from ASI 24001 and ASI 24004. The optimum temperature of mycelial growth was $25^{\circ}C$ and that of fruiting body development was $15{\sim}18^{\circ}C$. The color of pileus was yellowish brown and many scales and gelatinous sheath were on cap. Average number of individuals a bottle was 26 and its stipe was thick. P. adiposa can be produced by box cultivation using cotton waste.