• Title/Summary/Keyword: Pick-Up

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Effect of Early Pregnant Heifer as Donor on the Ovum Pick-Up Derived Oocyte Aspiration and Embryo Production (초기 임신우의 공란우 활용이 초음파 유도 난자 채취 및 수정란 생산에 미치는 영향)

  • Jin, Jong-In;Kwon, Tae-Hyeon;Choi, Byeong-Hyun;Kim, Sung-Soo;Jo, Hyun-Tea;Bang, Jae-Il;Kim, Sam-Chul;Cho, Kyu-Woan;Lee, Jung-Gyu;Kong, Il-Keun
    • Journal of Embryo Transfer
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    • v.26 no.1
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    • pp.19-25
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    • 2011
  • This study was carried out to evaluate the effect of early pregnant cow as donor for Ovum Pick-Up (OPU) derived oocyte aspiration and embryo production in Holstein heifers. Four non-pregnant and 2 pregnant Holstein heifers were used as donor and then carried out total 17 OPU session for 10 weeks (2 times per week). Recovered cumulus-oocyte-complexes (COCs) were classified into 4 grade by oocyte cytoplasm and cumulus cells and matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml FSH and 1 mg/ml estradiol in 5% $CO_2$ and over 99% humidity for 24 h. After 24 h co-incubation with post-thaw sperm, the presumed zygotes were cultured in CR1aa medium with 4 mg/ml BSA for 3 days and then changed CR1aa medium with 10% of FBS for another 3~4 days. The Mean number of aspirated follicles and collected oocytes in the early stage pregnant and non-pregnant heifers were $13.0{\pm}4.3$ and $10.6{\pm}3.9$, $5.4{\pm}3.4$ and $7.7{\pm}3.6$ per session, respectively. Rate of collected oocyte from aspirated follicles were 59.2% and 50.5%, respectively. The average number of good quality oocytes (Grade I and II) in the early stage pregnant and non-pregnant heifers was $3.7{\pm}2.7$ and $4.9{\pm}2.6$ (Mean${\pm}$SD). Cleavage and blastocyst developmental rates in Grade I and II were 22.2% and 25.5%, and then $1.7{\pm}0.9$ and $1.4{\pm}1.1$ blastocyst per session, respectively. In conclusion, OPU technology can be used in early stage pregnant and non-pregnant heifers without any problem and so applied OPU derived embryo production to maximize the ability of genetically valuable females.

Transforming Test Data of an Impact to a Crash Cushion into the Data of Different Impact Condition (충격흡수시설에 대한 특정 충돌시험데이터의 확대해석)

  • Jang, Dae Young;Ko, Man Gi;Joo, Jae Woong;Kim, Dong Sung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.4A
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    • pp.197-206
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    • 2012
  • It is found the first case of broad interpretation of the crash analysis in MASH (Manual for Assessing Safety Hardware, AASHTO, 2009) which is the guideline of roadside safety features in United States. They introduced the procedure of calculating 1,500 kg sedan safety index from the 2,270 kg pick-up truck crash test for crash cushion. First, following MASH's method, calculate 0.9 ton vehicle crash data and safety index using 1.3 ton vehicle crash test data and compare with actual 0.9 ton vehicle crash test data. results show that actual test data and the data calculated by MASH's method have great difference. Second, analyse the cause and develop new method. Proposed method can estimate not only the lighter vehicle (0.9 ton) crash data from the heavier vehicle (1.3 ton) crash test but also heavier vehicle (1.3 ton) data from lighter vehicle (0.9 ton) test. This method is superior to MASH's method and has stronger theoretical foundation. This paper proves the efficiency and the accuracy of new broad interpretation method using crash test data and investigates the principle.

Accuracy of Frozen Section Analysis of Sentinel Lymph Nodes for the Detection of Asian Breast Cancer Micrometastasis - Experience from Pakistan

  • Hashmi, Atif Ali;Faridi, Naveen;Khurshid, Amna;Naqvi, Hanna;Malik, Babar;Malik, Faisal Riaz;Fida, Zubaida;Mujtuba, Shafaq
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.4
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    • pp.2657-2662
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    • 2013
  • Background: Intraoperative sentinel lymph node biopsy has now become the standard of care for patients with clinically node negative breast cancer for diagnosis and also in order to determine the need for immediate axillary clearance. Several large scale studies confirmed the diagnostic reliability of this method. However, micrometastases are frequently missed on frozen sections. Recent studies showed that both disease free interval and overall survival are significantly affected by the presence of micrometastatic disease. The aim of this study was to determine the sensitivity and specificity of intraoperative frozen section analysis of sentinel lymph nodes (SLNs) for the detection of breast cancer micrometastasis and to evaluate the status of non-sentinel lymph nodes (non-SLNs) in those patients subjected to further axillary sampling. Materials and Methods: We performed a retrospective study on 154 patients who underwent SLN biopsy from January 2008 till October 2011. The SLNs were sectioned at 2 mm intervals and submitted entirely for frozen sections. Three levels of each section submitted are examined and the results were compared with further levels on paraffin sections. Results: Overall 40% of patients (62/154) were found to be SLN positive on final (paraffin section) histology, out of which 44 demonstrated macrometastases (>2mm) and 18 micrometastases (<2mm). The overall sensitivity and specificity of frozen section analysis of SLN for the detection of macrometastasis was found to be 100% while those for micrometastasis were 33.3% and 100%, respectively. Moreover 20% of patients who had micrometastases in SLN had positive non-SLNs on final histology. Conclusions: Frozen section analysis of SLNs lacks sufficient accuracy to rule out micrometastasis by current protocols. Therefore these need to be revised in order to pick up micrometastasis which appears to have clinical significance. We suggest that this can be achieved by examining more step sections of blocks.

Correlation between Pediatric Balance Scale and Gait Parameter in Children with Spastic Diplegic Cerebral Palsy (경직성 양하지 뇌성마비 아동의 소아균형검사와 보행변수 간의 상관관계)

  • Ko, Myung-Sook;Park, So-Yeon;Lee, Nam-Gi
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.10 no.4
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    • pp.251-257
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    • 2016
  • The Pediatric Balance Scale (PBS) was balance measurement equipment for school-age children with mild to moderate motor impairments. The aims of this study are to examine the correlation between PBS and spatiotemporal gait parameter and to identify the walking function with cerebral palsy through balance scale. The PBS consists of 14 items such as sitting of standing, standing to sitting, transfers, standing unsupported, standing on one foot, turning 360 degrees, turing to look behind, etc., and the spatiotemporal parameters include walking speed, stride length, step length, step width, cadence, double-limb support. All subjects were independently ambulatory children with spastic diplegic cerebral palsy, and they were assessed on PBS and spatiotemporal gait parameters by an experienced pediatric physical therapist. Pearson's correlation coefficient was used to assess the correlation between PBS and spatiotemporal gait parameters, and the level of significance was set at ${\alpha}$ = 0.05. Total score of PBS(r=.49~.58), standing to sitting(r=.48~.60), turning to look behind(r=.47~.53), and pick up object(r=.52~.69) were positively correlated with walking speed, stride length, step length, and cadence. Most items of the PBS were negatively correlated with double-limb support(r=-.48~-.92). These findings suggest that the pediatric balance scale can be applied to estimate gait function level for children with spastic diplegic cerebral palsy.

MATERIALS AND METHODS FOR TEACHING INTONATION

  • Ashby, Michael
    • Proceedings of the KSPS conference
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    • 1997.07a
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    • pp.228-229
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    • 1997
  • 1 Intonation is important. It cannot be ignored. To convince students of the importance of intonation, we can use sentences with two very different interpretations according to intonation. Example: "I thought it would rain" with a fallon "rain" means it did not rain, but with a fall on "thought" and a rise on "rain" it means that it did rain. 2 Although complex, intonation is structured. For both teacher and student, the big job of tackling intonation is made simpler by remembering that intonation can be analysed into systems and units. There are three main systems in English intonation: Tonality (division into phrases) Tonicity (selection of accented syllables) Tone (the choice of pitch movements) Examples: Tonality: My brother who lives in London is a doctor. Tonicity: Hello. How ARE you. Hello. How are YOU. Tone: Ways to say "Thank you" 3 In deciding what to teach, we must distinguish what is universal from what is specifically English. This is where contrastive studies of intonation are very valuable. Usually, for instance, division into phrases (tonality) works in broadly similar ways across languages. Some uses of pitch are also similar across languages - for example, very high pitch may signal excitement or urgency. 4 Although most people think that intonation is mainly about pitch (the tone system), actually accent placement (tonicity) is probably the single most important aspect of English intonation. This is because it is connected with information focus, and the effects on interpretation are very clear-cut. Example: They asked for coffee, so I made them coffee. (The second occurrence of "coffee" must not be accented). 5 Ear-training is the beginning of intonation training in the VeL approach. First, students learn to identify fall vs rise vs fall-rise. To begin with, single words are used, then phrases and sentences. When learning tones, the fIrst words used should have unstressed syllables after the stressed syllable (Saturday) to make the pitch movement clearer. 6 In production drills, the fIrst thing is to establish simple neutral patterns. There should be no drama or really special meanings. Simple drills can be used to teach important patterns: Example: A: Peter likes football B: Yes JOHN likes football TOO A: Mary rides a bike B: Yes JENny rides a bike TOO 7 The teacher must be systematic and let learners KNOW what they are learning. It is no good using new patterns and hoping that students will "pick them up" without noticing. 8 Visual feedback of fundamental frequency with a computer display can help students learn correct patterns. The teacher can use the display to demonstrate patterns, or students can practise by themselves, imitating recorded models.

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Survey on distribution and vegetation environment of Tricholoma matsutake in Kyonggi province, Korea (경기도지역 송이버섯 분포 및 식생환경조사)

  • Ha, Tai-Moon;Kim, Young-Ho;Chi, Jeong-Hyun;Ju, Young-Cheuol;Kim, Hee-Dong;Sung, Jae-Mo
    • Journal of Mushroom
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    • v.2 no.4
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    • pp.175-183
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    • 2004
  • We carried out survey of a pine mushroom picking district and its vegetation environment for increase and maintainment of its production in Kyonggi province. The district which we could pick up pine mushroom among the nineteen surveyed district were sixteen such as Geoleun ri Gangcheon myeon Yeoju gun Kyonggi do, etc. and total yields were 470~600 kg in Kyonggi province. The temperature of atmosphere and earth in Yeoju and Bonghwa were droped to $19^{\circ}C$ at 5~7th september and maintained from $12^{\circ}C{\sim}18^{\circ}C$ during the a pine mushroom picking period. The density of a pine tree per $100m^2$ was 5.1~27.1 trees, the years of it was 15~50years old and height was 4~11 meter. The pine tree in a pine mushroom picking district was competed with oak trees, azaleas, lacquers, etc. The depth of organic materials accumulated on the pine mushroom mycelium layer was 4.5 cm in Yeoju. The soil texture of a pine mushroom picking district was almost sandy, soil pH was 4.3~5.1 and organic material content was 3.91~8.28%.

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Underwater Noise Measurements on the Immersed Hydrofoil of High-Speed Vessel (고속 선박의 몰수된 hydrofoil에서 수중 소음 계측)

  • Park, Ji-Yong;Lee, Keun-Hwa;Seong, Woo-Jae
    • The Journal of the Acoustical Society of Korea
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    • v.30 no.1
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    • pp.9-16
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    • 2011
  • When a hydrofoil ship plies at high speed, there exist possibilities of collision with ocean mammals dwelling near the surface. An active sonar located within the immersed hydrofoil structure that provides the lift for the vessel, can be used for early warning of their presence. The proper functioning of the active sonar system depends on its ability to reject noise and pick up the target signal. In this article, we measured the noise on a hydrofoil of an operating ship with two flush-mounted hydrophones. The measurements were conducted for the purpose of (1) identifying the effect of operating state of machinery likes engine, cooler and generator (2) observing the change of noise depending on the measuring position (3) observing the change of noise with increasing ship speed. To verify our experiment, experiments were performed three times and the measured results are compared with other investigations and they show similarity to each other. The results are analyzed with frequency domain in order to apply to operating active sonar detecting system and focus on high frequency band within sonar's operating frequency region. Through these experiments and analysis, it is expected that we can identify the generated noise around hydrofoil where active sonar is installed and these results lead us to design active sonar that could distinguish target signal from noise more effectively.

The Design of IoT-based Drive Through Service System for Customers in Distribution Stores (대형 유통매장의 고객을 위한 IoT기반 드라이브 스루 서비스 시스템 설계)

  • Min, So-Yeon;Lee, Jong-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.11
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    • pp.151-157
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    • 2017
  • Recently, the retail industry has created efficient store operations, and has differentiated customer service through the future store. The intelligence of these stores is being applied by using technologies such as the Internet of Things (IoT), and the business process is being improved through this. The process also focuses on efficient store operations and service developments to provide customers with shopping convenience. The change in trends in the industry means that domestic distribution has already reached maturity. Even in countries where retail industries are mature, such as the U.S. and Europe, recent trends are moving toward maximizing operational efficiency and customer service. The reason is that many retailers have already reached saturation and survived the competition. This paper is a study of a drive-through service for automation and efficiency in receiving service after ordering by a customer of the distribution store. When ordering a product being purchased by a customer, the product picking process is done in a timely fashion through a picking scheduling agent. When the customer enters the store parking lot, a service supports the entry of information and finding a parking place so the customer can quickly pick up the goods. The proposed service can be applied to a retail store drive-through system, the distribution store's delivery system, the digital picking system, and indoor/outdoor large parking management systems, and it is possible to provide one-dimensional customer service through the application of IoT technology.

A Stimulated Acrosome Reaction Test as a Prognostic Factor in In Vitro Fertilization (체외수정시술시 예후 인자로서 정자 첨체반응 유발검사의 유용성)

  • Kim, Chung-Hoon;Chae, Hee-Dong;Kang, Eun-Hee;Chu, Hyung-Sik;Cheon, Yong-Pil;Kang, Byung-Moon;Chang, Yoon-Seok;Mok, Jung-Eun
    • Clinical and Experimental Reproductive Medicine
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    • v.25 no.3
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    • pp.251-260
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    • 1998
  • It is well known that the clinical test for responsibility of accurate fertilization capacity in male partners is very important to diagnose and treat the infertility. However, it has been reported that the traditional semen analysis cannot accurately predict fertilization and pregnancy potential. The present study was performed to evaluate the acrosomal reaction to ionophore challenge (ARIC) test as a prognostic indicator for fertilization of sperm and oocyte in an in vitro fertilization and embryo transfer (IVF-ET) program. From March 1996 to Februry 1997, 30 couples undergoing IVF program were allocated to this study group. All female partners in the study group were 35 years old or less and their serum level of basal follicle stimulating hormone (FSH) and estradiol $(E_2)$ were normal. All the male partners have normal parameters of semen analysis. The ARIC tests were performed on the day of ovum pick up and in vitro insemination in all the male partners. The controlled ovarian hyperstimulation (COH) using luteal long protocol of gonadotropin releasing hormone (GnRH) agonist was used in all couples for IVF-ET. The acrosomal reaction with $10{\mu}l$ of 10% DMSO was induced spontaneously in $10.1{\pm}9.8%$, and acrosomal reaction with calcium ionophore A 23187 was induced in $27.4{\pm}18.1%$, and the ARIC value was $17.4{\pm}16.2%$. There were no significant correlation between the ARIC value and the fertilization rate ($r^2$=0.044, p=0.268). There were also no significant correlation between the ARIC value and the percentage of the grade I, II embryos ($r^2$=0.046, p=0.261). On the basis of above results, it was suggested that ARIC test might not be a useful prognostic indicator for fertilization in IVF-ET in male partners with normal parameters of conventional semen analysis. We guessed that IVF-ET could be performed to the patients primarily without universal appilcation of ARIC test to all male partenrs, and if fertilization failure occurs, the micro assisted fertilization (MAF) such as intracytoplsmic sperm injection (ICSI) might be used as an alternative mode of treatment with acceptable success rate.

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제강분진을 이용한 침출수의 화학적 산화처리

  • 장윤영;강정우;정재현;배범한;박규홍;장윤석
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2001.04a
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    • pp.107-110
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    • 2001
  • 제철소에서 매년 대량 발생되어 주로 매립처분되고 있는 제강분진의 재활용 방안으로서, 폐수처리분야에 널리 사용되고 있는 펜톤산화공정의 반응촉매원인 Fe 공급원으로서 제강분 진의 활용 가능성에 대한 연구를 수행하였다. 본 연구에서는 포항제철소에서 제철부산물로 발생되는 제강분진을 전처리 없이 산화촉매로 사용하여 김포 수도권 매립지의 침출수 처리 공정에서 펜톤산화조에 유입되는 원수를 대상으로 과산화수소에 의한 산화처리 실험을 수행하였다. 반응은 회분식으로 수행하였으며, 일반적으로 알려진 펜톤산화반응의 주요 반응조 건인 운전 pH, 과산화수소 주입량 및 분할주입, 제강분진의 주입량 등의 변화에 따른 각 조 건별 시간에 따른 반응결과를 알아보았다. 또한 기존의 Fe 공급원으로 사용되고 있는 FeSO$_4$와 처리성능 및 적용조건에 대한 비교 실험도 수행하였다. 침출수 수질변화는 TOC (Total Organic Carbon) analyzer를 사용하여 측정한 TOC값으로 나타냈으며, pH controller 와 정량펌프를 사용하여 HCl과 NaOH주입을 통해 반응기간동안 일정 pH를 유지하였다. 본 연구결과, 최적 pH 조건인 4에서 최대 75% TOC 제거율을 나타내었으며, 대부분의 반응은 30분 이내에 이루어졌다. 주어진 실험조건에서 FeSO$_4$와 비교하여 반응속도와 처리효율에서 향상된 결과를 나타내었으며 반응 후 응집침전실험에서도 보다 높은 처리효과를 얻을 수 있었다. 결론적으로, 과산화수소/제강분진 시스템을 이용한 화학적 산화처리방법은 경제성과 처리성능에서 기존의 펜톤산화공정의 대체방안으로서 향 후 적용가능성이 높을 것으로 기대된다.g, 200 mg/kg, 300 mg/kg의 순서로 함량이 점차 감소하는 결과를 얻을 수 있었다. 이상의 결과를 종합하여 볼 때 가자 메탄올추출물은 PQ 유도독성을 신장 및 폐조직에서 효과적으로 경감시키는 것으로 나타났다.ted retailers ("sellers") must accept end-of-life items returned to them by the consumers. At the local level, Taipei City implements a pay-as-you-throw program, whereby citizens pay waste collection and treatment fees through the purchase of special trash bags approved by the Taipei City Government. However. recyclables that are separated by citizens are collected free-of-charge by the City. Taichung City and Kaohsiung City, on the other hand, enforce mandatory sorting schemes, whereby citizens face penalties if they don't separate recyclables from the trash before pick-up. These programs have resulted in a significant reduction in municipal waste. Per capita waste collected per day has dropped from 1.143

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