• Korean Journal of Environmental Agriculture
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• v.31 no.2
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• pp.122-128
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• 2012

BACKGROUND: Mine drainage from metal mining districts is a well-recognized source of environmental contamination. Oxidation of metal sulfides in mines, mine dumps and tailing impoundments produces acidic, metal-rich waters that can contaminate the local surface water and soil. METHODS AND RESULTS: This experiment was carried out to investigate the pollution assessment of heavy metal on the water quality of mine drainage, paddy soils and sediment in lower watershed affected by mine drainage of the Sambo mine. The average concentrations of dissolved Cd (0.018~0.035 mg/L) in mine drainage discharged from the main waste rock dumps(WRD) was higher than the water quality standards (0.01 mg/L) for agricultural water in Korea. Also, the average concentrations of dissolved Zn, Fe and Mn were higher than those of recommended maximum concentrations (Zn 2.0, Fe 5.0, Mn 0.2 mg/L) of trace metal in irrigation water proposed by FAO (1994). The average contents of Pb and Zn in paddy soils was higher than those of standard level for soil contamination(Pb 200, Zn 300 mg/kg) in agricultural soil by Soil Environmental Conservation Act in Korea. Also, the concentrations of Cd, Pb and Zn in sediment were higher than those of standard level for soil contamination (Cd 10, Pb 400, Zn 600 mg/L) in waterway soil by Soil Environmental Conservation Act in Korea. The enrichment factor (EFc) of heavy metals in stream sediments were in the order as Cd>Pb>Zn> As>Cu>Cr>Ni. Also, the geoaccumulation index (Igeo) of heavy metals in stream sediments were in the order as Zn>Cd>Pb>Cu>As>Cr>Ni, specially, the geoaccumulation index (Igeo) of Zn (Igeo 3.1~6.2) were relatively higher than that of other metals in sediment. CONCLUSION(s): The results indicate that stream water and sediment were affected by mine drainage discharged from the Sambo mine at least to a distance of 1 km downstream (SN-1, SN-2) of the mine water discharge point.

• Korean Journal of Ecology and Environment
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• v.49 no.1
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• pp.31-41
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• 2016

Deutzia paniculata is an endemic species to the Korean Peninsula. Despite of importance for conservation, the population structure and habitat characteristics of D. paniculata have not been determined yet. We analyzed the ecological characteristics of the species based on the literature review and field survey. Field survey was conducted on May to October 2014 during which 11 quadrats of size $15{\times}15m$ were studied in six regions. Each of the quadrats were further divided into $5{\times}5m$ small quadrats and population characteristics were recorded. The population and habitat characteristics were analyzed, including species abundance (density and coverage), demographic attributes (flowering rates and fruiting plants), vegetation (structure, species composition), light availability (transmitted light and canopy openness) and soil characteristics (temperature and humidity). We found that D. paniculata mainly distributed in Gyeongsangdo (including Taebaek in Gangwondo) along a broad elevational range of 290~959 m (mean: 493 m) above sea level. In preferred habitat the species grows within the slope range of $7^{\circ}$ and $35^{\circ}$ with the average of $16^{\circ}$. D. paniculata was generally distributed on talus deposits and low adjacent slopes. The average number of individual plants per small quadrat was 12.5 with the mean density $0.5stems\;m^{-2}$. The vegetative reproduction was frequent in D. paniculata and mean flowering rate was as low as 15%. Altogether 138 taxa were found in whole observation area with the dominant tree species mainly spring ephemerals, such as Cornus controversa (importance value: 25.5%) and Fraxinus rhynchophylla (importance value: 15.8%). Although, C. controversa usually grows on steep slopes and F. rhynchophylla mostly distributed at high-altitudes, however, both species distributed in disturbed environments and among talus deposits. Thus based on our results, we concluded that D. paniculata is a disturbance-prone species, primarily existing in habitats subjected to natural disturbances, such as floods. The species occurs less at anthropogenically disturbed sites, thus there is no apparent threat to the populations and habitat of D. paniculata.

• Korean Journal of Ecology and Environment
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• v.49 no.1
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• pp.11-21
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• 2016

Cymbidium kanran Makino is being threatened in its own habitats due illegal collecting and habitat changes by vegetation growth along historical landuse change. In this study, we established habitat restoration model for conservation of C. kanran based on ecological diagnosis. Through exploration to Jeju Island in 2014 and 2015, we identified 27 unknown habitats of C. kanran and in there, abiotic variables and vegetation structure and composition were quantified. Altitudinal distribution of C. kanran was between 200 m~700 m a.s.l. and compared to distribution in 2004, Area of Occupation (AOO) decreased at 82%. Specific habitat affinity was not observed by evenly found in mountain slope and valley and summergreen and evergreen broadleaved forests, but likely more abundant in valley habitats with higher soil and ambient moisture. Total of 96 individual of C. kanran was observed with an average density of $942.6individuals\;ha^{-1}$. The plants showed relatively short leaf length (average=$10.7cm{\pm}1.1cm$) and small number of pseudo bulbs ($1.2{\pm}0.2$). Flowering and fruiting individuals were not observed in field. C. kanran was classified into endangered plant species as CR (Critically Endangered) category by IUCN criteria. Phenotypic plasticity of C. kanran was likely support to sustain in more shaded habitat environment and recent habatat changes to closed canopy and low light availability may exhibit negatively effects to C. kanran's life history. Restoring C. kanran habitat should create open environment as grassland and low woody species density.

• Korean Journal of Ecology and Environment
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• v.49 no.1
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• pp.51-61
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• 2016

This study was carried out to assess the carbon budget of two urban parks and one natural park and to prepare the plan for improving the ecological functions of the park including carbon sink. Net Ecosystem Production (NEP) of those study sites was calculated from the relationship between Net Primary Production (NPP) and soil respiration of each study site. To understand carbon budget of the whole area designated as the park, carbon budget of the urban park was analyzed by classifying the vegetated and the non-vegetated zones. NEP of the Nohae and the Sanggye parks calculated by reflecting areal size that the non-vegetated zones occupy were shown in -1.0 and $0.6\;ton\;C\;ha^{-1}yr^{-1}$, respectively. On the other hand, NEP of Mt. Bulam natural park as a reference site was in $2.3\;ton\;C\;ha^{-1}yr^{-1}$. Based on the result, the Nohae park was assessed as carbon source rather than carbon sink. On the other hand, the Sanggye park was classified as carbon sink but the role was poor compared with natural park. The result is, first of all, due to lower NPP of the vegetation introduced for the parks compared with natural vegetation. The other reason is due to wide arrangement of non-vegetated zone. To solve those problems and thereby to create the urban park with high ecological quality, selection of plant species suitable for the ecological characteristic of the park, their arrangement imitated natural vegetation, and ecological zoning were recommended.

• Economic and Environmental Geology
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• v.48 no.6
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• pp.509-523
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• 2015

This study introduces newly implemented geological well logs database for Jeju public water wells, built for a research project focusing on integrated hydrogeology database of Jeju Island. A detailed analysis of the existing 1,200 Jeju Island geological logs for the public wells developed since 1970 revealed six major indications to be improved for their use in Jeju geological logs DB construction: (1) lack of uniformity in rock name classification, (2) poor definitions of pyroclastic deposits and sand and gravel layers, (3) lack of well borehole aquifer information, (4) lack of information on well screen installation in many water wells, (5) differences by person in geological logging descriptions. A new Jeju geological logs DB enabling standardized input and output formats has been implemented to overcome the above indications by reestablishing the names of Jeju volcanic and sedimentary rocks and utilizing a commercial, database-based input structured, geological log program. The newly designed database structure in geological log program enables users to store a large number of geology, well drilling, and test data at the standardized DB input structure. Also, well borehole groundwater and aquifer test data can be easily added without modifying the existing database structure. Thus, the newly implemented geological logs DB could be a standardized DB for a large number of Jeju existing public wells and new wells to be developed in the future at Jeju Island. Also, the new geological logs DB will be a basis for ongoing project 'Developing GIS-based integrated interpretation system for Jeju Island hydrogeology'.

• Korean Journal of Digital Imaging in Medicine
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• v.16 no.1
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• pp.13-19
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• 2014

Purpose: FLAIR image is beneficial for the diagnosis of various bran diseases including ischemic CVS, brain tumors and infections. However the border between the legion of brain metastasis and surrounding edema may not be clear. Therefore, this study aims to investigate the practical benefits of delayed imaging by comparing the image from a patient with brain metastasis before a contrast enhancement and the image 10 minutes after a contrast enhancement. Materials and methods: Of the 92 people who underwent MRI brain metastases in suspected patients 13 people in three patients there is no video to target the 37 people confirmed cases, and motion artifacts brain metastases in our hospital June-December 2013, 18 people measurement position except for the three incorrect patient (male: 11 people, female: 7 people, average age: 60 years) in the target, test equipment, 3.0T MR System (ACHIEVA Release, Philips, I was 8ChannelSENSE Head Coil use Best, and the Netherlands). TR 11000 ms, TE 125 ms, TI2800 ms, Slice Thickness 5 mm, gap 5 mm, is a Slice number 21, the parameters of the 3D FFE, T2 FLAIR variable that was used to test, TR 8.1 ms, TE 3.7 ms, Slice number 240 I set to. The experiment was conducted by acquiring the FLAIR prior to contrast enhancement (heretofore referred to as Pre FLAIR), and acquiring the 3D FFE CE five minutes after the contrast enhancement, and recomposing the images in an axial plane of S/T 3mm, G 0mm (heretofore referred to as MPR TRA CE). Using the FLAIR 10 minutes after the contrast enhancement (heretofore referred to as Post FLAIR) and Pi-View, a retrospective study was conducted. Using MRIcro on the image of a patient confirmed for his diagnosis, the images before and after the contrast media, as well as the CNR and SNR of the MPR TRA CE images of the lesion and the site absent of lesion were compared and analyzed using a one-way analysis of variance. Results: CNR for Pre FLAIR and Post FLAIR were 34.35 and 60.13, respectively, with MPR TRA CE at 23.77 showing no significant difference (p<0.050). Post-experiment analysis shows a difference between Pre FLAIR and Post FLAIR in terms of CNR (p<0.050), but no difference in CNR between Post FLAIR and MPR TRA CE (p>0.050), indicating that the contrast media had an effect only on Pre FLAIR and Post FLAIR. The SNR for the normal site Pre FLAIR was 106.43, and for the lesion site 140.79. Post FLAIR for the normal site was 107.79, and for the lesion site 167.91. MPR TRA CE for the normal site was 140.23 and for the lesion site 183.19, showing significant difference (p<0.050), and post-experiment analysis shows that there was a difference in SNR only on the lesion sites for Pre FLAIR and Post FLAIR (p<0.050). There was no difference in SNR between the normal site and lesion site for Post FLAIR and MPR TRA CE, indicating no effect from the contrast media (p>0.050). Conclusions: This experiment shows that Post FLAIR has a higher contrast than Pre FLAIR, and a higher SNR for lesions, It was not not statistically significant and MPR TRA CE but CNR came out high. Inspection of post-contrast which is used in a high magnetic field is frequently used images of 3D T1 but, since the signal of the contrast medium and the blood flow is included, this method can be diagnostic accuracy is reduced, it is believed that when used in combination with Post FLAIR, and that can provide video information added to the diagnosis of brain metastases.

• Journal of Embryo Transfer
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• v.21 no.3
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• pp.263-271
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• 2006

The purpose of this study was undertaken to compare ability of frozen-thawed sperm characteristics between two strains (miniature pig and Duroc). The semen was collected by gloved-hand method into a pre-warmed ($37^{\circ}C$) thermos bottle. The semen was diluted with same volume extender and added to LEY solution for freezing. The diluted semen was placed in 0.5 ml straws, and freezing was initiated by exposing the straws to liquid nitrogen ($LN_2$) vapours for 10 min before placing them into $LN_2$ for cryopreservation. The frozen-semen straw were thawed at 20, 37 and $50^{\circ}C$ for 1 min, 45 sec and 10 sec within water-bath. The semen sample were evaluated at 0, 3, 6, 9, and 12 h after incubation at $37^{\circ}C$ for analysis of sperm ability. Abnormality of spermatozoa in miniature pig was significantly (p<0.05) higher than that in Duroc at 0, 9 and 12 h of post-thawing incubation after frozen-thawing. The percentage of F-patterned spermatozoa in miniature pig was significantly (p<0.05) lower, while the percentage of AR (acrosome reacted spermatozoa) pattern was higher in the miniature than in the Duroc. On the other hand, there was no significant difference in the viability of spermatozoa thawed at different temperature ($20^{\circ}C\;and\;37^{\circ}C$) between two species, but the viability in miniature pig was higher (p<0.05) than in Duroc when sperm was thawed at $50^{\circ}C$. In conclusion, this study suggest that suitable freezing method for miniature pig semen is required for increasing post-thawing viability and fertilization capacity.

• Korean Journal of Environmental Agriculture
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• v.24 no.3
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• pp.222-231
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• 2005

Most of the tailings have been left without any management in abandoned metalliferous mines and have become the main source of heavy metal contamination of agricultural soils and crops in the these areas. To compare of environmental assessment of heavy metals in tailings derived from various 25-metalliferous mines in Korea, 3 different analysis methods such as water soluble, 0.1 M-HCl extractable, and total acid digestion method (aqua regia) were used. The chemical composition of water soluble in mine tailing were in the order ${SO_4}^{2-}>Ca^{2+}>Mn^{2+},\;Na^+,\;Al^{3+}>Mg^{2+},\;Fe^{3+}>Cl^-$. Specially, pH, EC, ${SO_4}^{2-},\;and\;Ca^{2+}$ concentrations in tailing varied considerably among the different mines. The average total concentrations of Cd, Cu, Pb, Zn, and As in tailing were 31.8, 708, 4,961, 2,275 and 3,235 mg/kg, respectively. Specially, the contents of Cd, Zn and As were higher than those of countermeasure values for soil contamination (Cd : 4, Zn : 700 and As : 15 mg/kg in soil) by Soil Environmental Conservation Act in Korea. The rates of water soluble heavy metals to total contents in tailings were in the order Cd > Zn > Cu > Pb > As. The rates of 0.1M-HCl extractable Cd, Cu, Pb, Zn, and As (1M-HCl) to total content were 17.4, 10.2, 6.5, 6.8 and 11.4% respectively. The enrichment factor of heavy metals in tailings were in the order As > Pb > Cd > Cu > Zn. The pollution index in tailing Au-Ag mine tailing were higher than those of other mine tailing. As a results of enrichment factor and pollution index for heavy metal contaminations in mine tailing of metalliferous mines, the main contaminants are mine waste materials including tailings.

• Journal of Life Science
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• v.27 no.2
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• pp.225-232
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• 2017

This study aimed to evaluate several biological activities of Pharbitis nil and to isolate an anticancer agent from its methanol extract. Pharbitis nil seeds were extracted with methanol (PNM). Then, PNM was fractionated into solvent layers such as ethyl acetate fraction (PNE), butanol fraction (PNB), and water fraction (PNW). The biological activities of the fractions were analyzed for tyrosinase inhibition, lipase inhibition, DPPH-free radical scavenging, and cell growth inhibition. PNM showed strong growth inhibition of prostate cancer PC-3 cells. PNM was subjected to Diaion HP-20 and eluted stepwise with 50%, 80%, and 100% methanol. Then, for activity-guided fraction, each fraction was analyzed for growth inhibition of prostate cancer PC-3 cells by using an MTT assay. Because the 100% fraction showed significantly strong inhibitory activity, the fraction was further separated in the reverse phase C18, which was eluted with 80% and 90% methanol. The 90% fraction was further subjected to Sephadex LH-20 using a mobile solvent of 100% methanol. Finally, the compound PN was partially purified for HPLC analysis. PN showed cell growth inhibitory activity and induced the apoptosis and cell cycle arrest of prostate cancer PC-3 cells, as measured by flow cytometry. The results together suggest that Pharbitis nil possesses various biological activities, especially the inhibitory activity for the proliferation of prostate cancer PC-3 cells, suggesting the possibility of its use as an anticancer agent.

• Clinical and Experimental Reproductive Medicine
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• v.23 no.3
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• pp.303-310
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• 1996

The objective of this study was to examine the effect of embryos development following IVF of in vitro-matured porcine oocytes treated with epidermal growth factor (EGF). When cumulus-enclosed oocytes were incubated in TCM 199 medium supplemented with (1) control group, (2) 10 ng/ml EGF, (3) 10${\mu}g$ml FSH and 10% FBS, or (4) 10 ng/ml EGF, 10 ${\mu}g$/ml FSH, and 10% FBS for 42 hr, the late developmental rates on NCSU (0.4% BSA) medium after fertilization were higher in (3) and (4) groups (13.4, 18.3%) than in (2) group (5.2%, p < 0.005), but (2) group is significantly higher than the development to blastocyst of oocytes of (1) group (1.2%). Also, when the cell number of total, ICM, and TE of those blastocysts at 6 day produced in vitro was investigated by double staining (PI and bisbenzimide), total cell number of (4) group (58.80${\pm}$ 11.90) was higher than that of (2) and (3) groups (42.17${\pm}$9.97, 49.07${\pm}$9.77, P < 0.05). ICM cell number of blastocysts of (4) group (11.69${\pm}$5.56) was higher than that of (2) and (3) groups (5.00${\pm}$4.24, 6.77${\pm}$4. 92, P < 0.05). Furthermore, the proportion of ICM in (4) group (19.0${\pm}$1.6) was higher than that in (2) and (3) groups (11.1${\pm}$3.0, 12. 7${\pm}$2.1). These results suggested that in vitromatured porcine oocytes treated with EGF alone can be developed to blastocyst, but high proportion on the development to blastocyst and number of total cell and ICM in blastocyst can be obtained when supplemented with additional FSH and FBS.